Search tips
Search criteria 


Logo of jclinpathJournal of Clinical PathologyVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
J Clin Pathol. 1997 February; 50(2): 153–156.
PMCID: PMC499742

Comparative analysis of biotin intranuclear inclusions of gestational endometrium using the APAAP, ABC and the PAP immunodetection systems.


AIMS/BACKGROUND: Intranuclear inclusions have been observed in gestational endometrial glands. Although resembling Herpes simplex virus infected cells, these nuclei have been shown to contain endogenous biotin. Hence, immunohistochemical systems using the avidin-biotin complex will result in cross reaction and false positivity. This study investigates a series of 10 gestational endometria (formalin fixed and paraffin wax embedded) with intranuclear inclusions using three different immunodetection systems with primary anti-biotin. RESULTS: Both the alkaline phosphatase anti-alkaline phosphatase (APAAP) and peroxidase anti-peroxidase (PAP) systems confirmed that the intranuclear inclusions were endogenous biotin. The streptavidin biotin complex (StreptABC) system produced a positive reaction in both test sections and controls (omitting primary anti-biotin) in the presence of prior blocking with free avidin and biotin. In addition, aberrant immunoreactivity was observed in adjacent nuclei/cytoplasm of endometrial glands and decidualised stroma in the negative control of the PAP system. This was subsequently eliminated using microwave pretreatment prior to immunohistochemistry. CONCLUSION: The use of either the APAAP or PAP immunodetection systems (the latter with microwave pretreatment) is recommended for any immunohistochemical or non-isotopic in situ hybridisation investigation undertaken on gestational endometria.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Wood GS, Warnke R. Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems. J Histochem Cytochem. 1981 Oct;29(10):1196–1204. [PubMed]
  • Yokoyama S, Kashima K, Inoue S, Daa T, Nakayama I, Moriuchi A. Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium. Am J Clin Pathol. 1993 Jan;99(1):13–17. [PubMed]
  • Sickel JZ, di Sant'Agnese PA. Anomalous immunostaining of 'optically clear' nuclei in gestational endometrium. A potential pitfall in the diagnosis of pregnancy-related herpesvirus infection. Arch Pathol Lab Med. 1994 Aug;118(8):831–833. [PubMed]
  • Wright CA, Haffajee Z, van Iddekinge B, Cooper K. Detection of herpes simplex virus DNA in spontaneous abortions from HIV-positive women using non-isotopic in situ hybridization. J Pathol. 1995 Aug;176(4):399–402. [PubMed]
  • DAKSHINAMURTI K, MISTRY SP. Tissue and intracellular distribution of biotin-C-1400H in rats and chicks. J Biol Chem. 1963 Jan;238:294–296. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group