PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jidLink to Publisher's site
 
J Infect Dis. 2016 September 15; 214(6): 925–934.
Published online 2016 May 13. doi:  10.1093/infdis/jiw201
PMCID: PMC4996146

Streptococcus oralis and Candida albicans Synergistically Activate μ-Calpain to Degrade E-cadherin From Oral Epithelial Junctions

Abstract

Streptococcus oralis forms robust mucosal biofilms with Candida albicans that have increased pathogenic potential. In this study, using oral epithelial cultures, organotypic oral mucosal constructs, and a mouse model of oral infection, we demonstrated that S. oralis augmented C. albicans invasion through epithelial junctions. C. albicans and S. oralis decreased epithelial E-cadherin levels by synergistically increasing µ-calpain, a proteolytic enzyme that targets E-cadherin. In the mouse coinfection model this was accompanied by increased fungal kidney dissemination. Coinfection with a secreted aspartyl protease (sap) mutant sap2456 and S. oralis increased μ-calpain and triggered mucosal invasion and systemic dissemination, suggesting that fungal protease activity is not required for invasion during coinfection. We conclude that C. albicans and S. oralis synergize to activate host enzymes that cleave epithelial junction proteins and increase fungal invasion.

Keywords: S. oralis, C. albicans, calpain, synergy, E-cadherin, oral mucosa

Candida albicans is a core component of the human oral mycobiome in health [1, 2]. Most oral fungal infections are superficial and caused by C. albicans under host-permissive conditions [3]. In severe immunosuppression C. albicans can invade epithelial barriers and reach the bloodstream, resulting in life-threatening systemic infections [4]. C. albicans uses 2 major mechanisms to compromise epithelial barriers: induced endocytosis and active penetration [5, 6]. Active penetration includes intracellular penetration and paracellular invasion through cell junctions [5, 7, 8]. Previous studies have shown that proteolytic degradation of E-cadherin in adherens junctions compromises epithelial barrier function and promotes C. albicans invasion [7, 9].

As most oral infections, the pathoecology of oropharyngeal candidiasis is complex and may involve bacteria such as streptococci, which are abundant colonizers in health and coexist with fungi in oral biofilms [3, 10]. C. albicans and oral streptococci interact via several molecular mechanisms, resulting in increased biofilm growth and pathogenic synergy [11, 12]. For example, using mucosal tissue constructs we showed that C. albicans promoted Streptococcus oralis biofilm growth, which in turn increased invasion of the oral and esophageal mucosa by C. albicans [13]. Furthermore, we demonstrated that coinfection of mice with S. oralis and C. albicans augmented oral thrush lesions and mucosal neutrophilic infiltration [14].

Similar to C. albicans paracellular invasion, increased neutrophil transmigration requires disassembly of epithelial cell junctions. In airway mucosa, disassembly of epithelial junctions for polymorphonuclear leukocyte migration is controlled by activation of μ-calpain (calpain 1) and m-calpain (calpain 2) [15]. These are calcium ion–dependent cysteine proteases involved in many cell processes [16], and their activities are strictly regulated in vivo. Activated calpain 1 and/or calpain 2 cleave E-cadherin and occludin in cell junctions to promote recruitment of polymorphonuclear leukocytes into the airways [15]. Because E-cadherin degradation is involved in neutrophil transmigration and C. albicans tissue invasion, and both processes are increased during coinfection, we hypothesized that C. albicans and S. oralis synergize to activate calpain and degrade E-cadherin. We provide evidence for the first time that this type of host modulation is mediated by synergy between 2 major representatives of the core bacterial and fungal oral microbiota.

MATERIALS AND METHODS

Strains and Growth Conditions

C. albicans SC5314 is a bloodstream isolate [17] that forms robust oral biofilms [10]. C. albicans Δsap2456 (M31) and reference strain CAI4-URA3 were described in reference [18]. C. albicans was maintained in yeast extract peptone dextrose agar and grown in yeast extract peptone dextrose broth, aerobically, at room temperature, on a shaker. S. oralis 34, S. oralis 108, Streptococcus gordonii Challis CH1, and Streptococcus mitis (American Type Culture Collection 49456) were grown in brain heart infusion medium (Oxoid), under aerobic, static conditions at 37°C and 5% carbon dioxide. To prepare nonviable cells, suspensions of yeast cells (108/mL) or bacterial cells (109/mL) were produced from overnight broth cultures or subcultures and heated at 95°C for 10 minutes or treated with 100 mmol/L thimerosal or 10% formalin for 1 hour, followed by extensive washing with phosphate-buffered saline [8, 19].

Mouse Oral Infection

The mice used were 6–12-week-old female C57BL/6 mice, purchased from Jackson Laboratories (animal protocol 100358-0215). The C. albicansS. oralis mouse coinfection model has been described in detail elsewhere [14]. Briefly, mice were immunosuppressed with cortisone acetate (225 mg/kg, administered subcutaneously), on the first and third days after infection. A cotton pellet, saturated with 100 µL of microbial suspension (yeast cells [6 × 108/mL] and/or bacteria [2.5 × 109/mL]), was placed subglossally, in mice under anesthesia for 2 hours. Fresh suspensions of microorganisms were added daily in drinking water. Mice were killed on day 4 or 5 after inoculation.

RNA Extraction and Reverse-Transcription Quantitative Polymerase Chain Reaction

The mouse tongues were homogenized using a POLYTRON homogenizer, and the supernatants were beat by zirconia beads (Ambion). RNA was purified using the QIAgen RNeasy Mini Kit. RNA concentrations and quality were determined using a NanoDrop device (Thermo Scientific). Complementary DNA was synthesized with SuperScript III CellsDirect cDNA Synthesis kits (Invitrogen). Reverse-transcription quantitative polymerase chain reaction was performed with a Bio-Rad CFX96 cycler and the IQTM SYBR Green Supermix kit (Bio-Rad). Primer sequences for mouse genes were from PrimerBank [20, 21]. Sequences for fungal genes are shown in Supplementary Table 1.

Infection of Oral Mucosal Organotypic Constructs

Oral mucosal analogues that mimic keratinized (gingival) or nonkeratinized (floor of the mouth) human oral mucosa have been described elsewhere [22]. Briefly, the constructs consist of TERT-2–immortalized human oral keratinocytes (gingival, OKG4; floor of the mouth, OKF6) seeded on collagen type I–embedded fibroblasts (3T3). Tissues are airlifted for epithelial differentiation and stratification. Tissues were inoculated with 106 cells of C. albicans, 107 cells of streptococci, or a combination and incubated at 37°C in a 5% carbon dioxide incubator for 16 hours. Some tissues were pretreated overnight with calpeptin (40 µmmol/L; Millipore), a calpain inhibitor. In some experiments, recombinant human calpain 1 protein (Calbiochem; 40 µg/mL) was used with Candida.

Immunofluorescence Imaging

Immunofluorescence staining was described elsewhere [10, 13, 14]. Briefly, tissue sections or monolayers of epithelial cells (OKF6 or Madin-Darby canine kidney) were stained with anti-E-cadherin polyclonal antibody (BD Biosciences), rabbit anti–calpain 1 polyclonal antibody (Abcam), mouse anti–calpain 1 monoclonal antibody (Abcam), or the appropriate isotype control, followed by a fluorescein isothiocyanate– or tetramethylrhodamine isothiocyanate–labeled secondary antibody. Epithelial nuclei were visualized using Hoechst 33258 or Syto9 stain (Invitrogen). To visualize epithelial nuclei, cytoplasmic actin, and C. albicans simultaneously, Hoechst 33258 (or Syto9), tetramethylrhodamine isothiocyanate–Phaloidin (Sigma), and a fluorescein isothiocyanate–labeled anti-Candida antibody (Meridian Life Science) were used, respectively. S. oralis and S. mitis were visualized by means of fluorescence in situ hybridization with the Streptococcus-specific oligonucleotide probe STR405, conjugated to Alexa 546 [12]. Some tissues were visualized with a Zeiss LSM 780 confocal scanning laser microscope equipped with an argon (488- and 543-nm) laser, using a water immersion C-Apochromat ×40 objective. Other fluorescence images were captured using a Zeiss Axio Imager M1 microscope, using a ×20 objective. Candida cells penetrating into epithelial cells were quantified using the software BioImageXD [23]. The total fungal invasion area and surface biomass were quantified using the IMARIS 7.0 software package [24].

Western Blotting

Tongues were homogenized in lysis buffer [13]. To prepare epithelial monolayer lysates, 4 × 105 OKF6/TERT-2 cells per well were seeded in 6-well plates in complete keratinocyte serum-free medium (Invitrogen) and incubated overnight. The next day, cells were challenged with 4 × 105 heat-killed C. albicans, 4 × 106 heat-killed S. oralis, or a combination, in unsupplemented keratinocyte serum-free medium. Cell lysates were prepared after 48 hours. Total protein was quantified with Pierce BCA Protein Assay (Fisher Scientific), and 40 µg of protein was loaded per lane. After electrophoresis, transfer and blocking, membranes were incubated with goat anti-mouse (R&D) or anti-human E-cadherin (BD Biosciences) or rabbit anti–calpain 1 (Abcam). Glyceraldehyde-3-phosphate dehydrogenase or β-actin was used as an internal loading control. Proteins were detected with Western blot chemiluminescence reagents (Bio-Rad), according to the manufacturer's instructions. The signal density was measured with ImageJ software (National Institutes of Health; https://imagej.nih.gov/ij/).

Calpain Activity Assay

OKF6/TERT-2 cells were seeded on 24-well plates (105 cells per well) overnight, and the next day cells were challenged with 105 heat-killed, formalin-fixed or thimerosal-killed C. albicans, 106 heat-killed S. oralis, or their combination. T-butoxy-carbonyl-L-leucyl-L-methionine amide (20 µmol/L; Molecular Probes), a calpain-specific fluorogenic substrate, was added, and fluorescence was observed microscopically. To quantify fluorescence in a microplate reader, cells were lysed in 200-µL water/well. In some wells, calpeptin (40 µmol/L) or MDL-28179 (50 µmmol/L; Santa Cruz Biotech), a cell-permeable calpain-selective inhibitor, was added 30 minutes before microbial cells as a negative control. Under these conditions, inhibitors were not toxic to epithelial cells, as tested using a lactate dehydrogenase cytotoxicity assay (not shown).

Release of Soluble E-cadherin From Cell Junctions

OKF6/TERT-2 cells were incubated for 30 hours with heat-killed organisms as above, and supernatants were assayed for soluble E-cadherin with enzyme-linked immunosorbent assay, following the manufacturer's instructions (R&D). In some wells, cells were pretreated with calpain inhibitors for 30 minutes to test whether E-cadherin release was calpain mediated. E-cadherin release was also quantified in subnatants collected from mucosal constructs.

Statistical Analyses

Data were analyzed for statistically significant differences with Student t test, using GraphPad Prism software (version 6). Statistical significance for all tests was set at P < .05.

RESULTS

Oral Mucosal Invasion by C. albicans During Coinfection

Previous work showed that S. oralis augments invasion of C. albicans SC5314 into the submucosal compartment of stratified, nonkeratinized oral and esophageal mucosal constructs, formed by TERT-2–immortalized keratinocytes [13, 24]. To extend these findings, we tested whether constructs formed by OKG4/TERT-2 cells, representing the keratinized gingival mucosa, are also more permissive to C. albicans invasion in the presence of S. oralis. As seen in Figure Figure11A, when inoculated alone, C. albicans forms a biofilm on the mucosal surface with a portion of the biofilm organisms invading the multilayer construct. However, when coinoculated with S. oralis 34 (Figure (Figure11A) or S. oralis 108 (not shown), the entire C. albicans biomass was observed invading through the mucosal layers into the submucosal compartment. When inoculated alone, neither S. oralis strain formed a biofilm on mucosal analogues, consistent with previous findings [13, 24].

Figure 1.
Streptococcus oralis increases Candida albicans oral mucosal invasion in vitro and in vivo. A, B, Mucosal analogues (A) or tongue tissues (B) infected with S. oralis 34 (So), C. albicans SC5314 (Ca) or both in combination (CaSo). Mucosal analogues were ...

To examine Candida invasion in vivo, we examined the mouse dorsal tongue surface. When each organism was inoculated alone, 4 days after infection Candida remained on the surface of the keratin layer (arrows in Figure Figure11B). However, when coinoculated with S. oralis, C. albicans formed robust biofilms, and hyphal organisms invaded more deeply into the mucosal layers. Interestingly, a large number of hyphae were observed within intercellular spaces (arrows). Compared with monoinfected mice, coinfected mice had higher C. albicans colony-forming unit counts in tongue tissues and greater systemic spread into the kidneys at 4 and 5 days, respectively (Figure (Figure11C). Consistent with previous work [14], S. oralis was not recovered from tongues, unless the mice were coinoculated with C. albicans. Despite high recovery rates in coinfected mice, S. oralis did not invade into the mucosa or spread into the kidneys (Figure (Figure11B and and11C).

Oral Mucosal E-cadherin Protein Levels in Coinfection

To begin to elucidate the mechanism of fungal invasion, we infected oral mucosal organotypic constructs with the 2 organisms and, using a triple immunofluorescence stain, we traced the position of invading hyphae within the stratified epithelium. Using confocal microscopy, we noticed that C. albicans hyphae invaded the mucosal compartment predominantly through intercellular spaces (Figure (Figure22A). In coinfected tissues, only 5.3% of C. albicans cells that had invaded the mucosal compartment colocalized with actin with the remaining cells invading paracellularly, compared with 20.5% of the cells in monoinfection (Figure (Figure22B). This suggested that coinfection with S. oralis promoted paracellular Candida invasion. Paracellular invasion by C. albicans requires disassembly of epithelial junction proteins [7, 25]. Thus, we asked whether E-cadherin was reduced in the presence of the 2 organisms. E-cadherin signal in mucosal constructs infected with S. oralis only was indistinguishable from uninfected tissues (Figure (Figure22B). Tissues infected with C. albicans exhibited localized loss of E-cadherin, probably owing to proteolysis mediated by fungal aspartyl proteases [7]. Interestingly, when infected with both organisms, the E-cadherin signal was significantly reduced throughout the epithelial layers, suggesting a new mechanism of E-cadherin modulation (Figure (Figure22B).

Figure 2.
Oral mucosal E-cadherin is significantly reduced in vitro in coinfection. A, Confocal image of an oral mucosal construct infected with both organisms. Tissues were stained with a fluorescein isothiocyanate–labeled anti-Candida antibody (green ...

We then asked whether costimulation with C. albicans and S. oralis can decrease cell-associated E-cadherin protein in a confluent cell monolayer. To prevent E-cadherin degradation mediated by fungal proteases, we used heat-killed organisms. As shown in Figure Figure22C, bacterial or fungal challenge alone did not significantly affect E-cadherin, whereas the combination of C. albicans and S. oralis triggered a significant reduction. To confirm that E-cadherin was released from cell junctions, soluble E-cadherin was quantified by enzyme-linked immunosorbent assay in culture supernatants. Results showed that S. oralis alone increased soluble E-cadherin release by 41%, C. albicans alone by 57.7% and the combination of the 2 organisms by 104.5% over basal, unstimulated levels (Figure (Figure22D). Importantly, the calpain-selective inhibitor MDL-28179 completely inhibited the release of soluble E-cadherin triggered by these organisms.

Similarly, in mice receiving combined oral inoculation with C. albicans and S. oralis E-cadherin was almost completely absent in intercellular junctions of the tongue mucosa (Figure (Figure33A), and this was confirmed by Western blot analysis of tongue lysates (Figure (Figure33B). To rule out the possibility that E-cadherin messenger RNA transcription was reduced owing to increased tissue destruction, we performed reverse-transcription quantitative polymerase chain reaction and found that E-cadherin messenger RNA expression was not significantly altered in any of the infection groups, compared with uninfected mice (Figure (Figure33C). Together, these data suggested that C. albicans and S. oralis may manipulate the epithelial cell response to weaken the mucosal barrier via reduction of E-cadherin in intercellular junctions and that this process is calpain mediated.

Figure 3.
Oral mucosal E-cadherin is significantly reduced in coinfected mice. A, Overlay images of tongue tissue sections stained for E-cadherin (green) and counterstained with nucleic acid stain Hoechst 33258 (blue). Mice were infected for 4 days. Bars represent ...

Calpain Protein Activity in Oral Epithelium During Coinfection

Certain bacteria can reduce E-cadherin protein levels in intercellular junctions by activating calpain 1 and 2 proteins in airway or gastric epithelial cells [15, 26]. We thus reasoned that costimulation of oral epithelial cells with C. albicans and S. oralis may increase calpain protein and/or activity levels. To test this hypothesis, we first challenged epithelial (OKF6 and Madin-Darby canine kidney) monolayers with heat-killed C. albicans and S. oralis and observed cell fluorescence after adding a cell-permeable fluorogenic calpain substrate. A low basal level of calpain activity was present in oral epithelial cells, which increased somewhat with either organism alone (Figure (Figure44A and Supplementary Figure 1). However, simultaneous challenge with both organisms triggered a significant increase in calpain fluorescence. To confirm microscopic observations, we quantified calpain fluorescence intensity using a fluorescence reader. The 2 organisms synergistically increased calpain activity, indicated by the significant increase in fluorescence intensity over the sum of fluorescence with each microorganism alone (Figure (Figure44B). Calpain activity triggered by Candida and S. oralis did not differ significantly between thimerosal- or formalin-treated yeast and hyphae (Supplementary Figure 2). Interestingly, the combination of C. albicans with the phylogenetically related organisms S. gordonii or S. mitis did not synergistically increase calpain activity. Consistent with this observation, when tested in organotypic constructs, S. mitis did not significantly increase the invasive potential of Candida (Supplementary Figure 3).

Figure 4.
Candida albicans and Streptococcus oralis synergistically increase calpain protein activity in oral epithelium. A, Fluorescence images of epithelial cells (OKF6/TERT-2) stained with a cell-permeable fluorogenic calpain substrate (blue), anti-E-cadherin ...

To explore whether calpain 1 was involved in this process we immunoblotted calpain 1 using an antibody that can detect both the full-length and the cleaved, active forms of the enzyme. As seen in Figure Figure44C, challenge of oral epithelial monolayers with the 2 organisms in combination mostly increased levels of the active 75-kDa form of calpain 1, whereas each organism alone had a lower effect.

To extend these observations in mucosal tissues, organotypic constructs and mouse tissues were stained for calpain 1 and E-cadherin protein expression using immunofluorescence. As shown in Figure Figure55A, when infected with both microorganisms, the calpain 1 signal was significantly intensified at the superficial mucosal layers where the E-cadherin signal was reduced, compared with monoinfection. In mice, monoinfection triggered weak calpain 1 fluorescence throughout the entire mucosa. In coinfection the intraepithelial calpain 1 fluorescence was stronger, especially in areas in contact with microbial biofilms (Figure (Figure55B). These results were corroborated by Western blot analysis, which showed increased calpain 1 in coinfected mice (Figure (Figure55C). The 75-kDa band, corresponding to the active protein, was not detectable in mouse tongue lysates because its concentration per cell is very low in vivo and varies according to tissue or organ type [27, 28]. Taken together, these data indicate that during oral infection C. albicans and S. oralis synergize to trigger a significant host epithelial modulation via increase in calpain 1.

Figure 5.
Calpain 1 is increased in mucosal constructs and tongue tissues during coinfection. A, Immunofluorescence stain for calpain 1 (red) and E-cadherin (green) in OKF6/TERT-2 mucosal constructs after 16 hours of infection. Arrows indicate increased red and ...

Role of Secreted Aspartyl Proteases in Increased Fungal Invasion During Coinfection

Previous work demonstrated that secreted aspartyl proteinases, particularly Sap5, are involved in the proteolytic degradation of E-cadherin during Candida paracellular invasion [7]. Because S. oralis increased C. albicans burden in mouse tongues, we reasoned that sap gene expression may also be elevated in coinfected mice, thus contributing to mucosal invasion. We screened expression of all Candida protease genes (sap1-10) and found that sap2 and sap4–6 gene expression was increased in coinfection relative to monoinfection (Figure (Figure66A). To examine the role of these proteases in mucosal invasion we used a sap2,4,5,6 mutant, with or without S. oralis, to infect oral mucosal organotypic constructs and mice. As expected from previous findings [7], the mutant formed invasion-deficient biofilms on mucosal constructs. However, in the presence of S. oralis this mutant invaded past the basal epithelial layer into the submucosa (Figure (Figure66B). To clarify the mechanism of invasion, we examined calpain 1 and E-cadherin expression in the mucosal constructs using double immunofluorescence staining. As expected, mucosal constructs coinfected with S. oralis and the Δsap2456 strain showed increased calpain 1 and decreased E-cadherin immunofluorescence, compared with the mutant alone (Figure (Figure66C), suggesting that invasion is mediated by calpain 1.

Figure 6.
Fungal secreted aspartyl proteases are not required for invasion during coinfection. A, Candida Sap1–10 gene messenger RNA levels were detected in mouse tongue tissues by reverse-transcription quantitative polymerase chain reaction. Results represent ...

Oral fungal burdens in mice infected with the Δsap2456 mutant (with or without S. oralis) were attenuated at day 4 after infection compared with the reference strain (not shown), consistent with other reports [29]. However, high numbers of the Δsap2456 mutant were recovered from the tongues and kidneys of all mice coinfected with S. oralis, at days 4 and 5 after infection, respectively, demonstrating that proteases Sap 2,4–6 are not required for fungal colonization, invasion and systemic dissemination when mice are coinfected with streptococci (Figure (Figure66D).

Role of Epithelial Calpain Activity in Increased Mucosal Fungal Invasion

E-cadherin is cleaved by exogenously added calpain 1, and endogenous calpain activity can be inhibited by the tissue-permeable inhibitor calpeptin [30]. We therefore first tested whether, when added to C. albicans–infected mucosal constructs, calpain 1 could promote E-cadherin release and fungal invasion. In Candida-infected constructs calpain 1 triggered E-cadherin release similar to C. albicansS. oralis–coinfected tissues (Supplementary Figure 4). Because strain CAI4-URA3 was highly invasive on its own, the additional effect of exogenous calpain 1 on invasion was small (Figure (Figure77A and Supplementary Figure 5). However, calpain 1 triggered a massive invasion of the invasion-deficient Δsap2456 strain into the mucosal constructs. Next, we questioned whether the calpain inhibitor calpeptin can reduce fungal invasion in C. albicansS. oralis–coinfected constructs. Calpeptin significantly reduced invasion of both reference and Δsap2456 strains in the presence of S. oralis, consistent with the significant reduction of E-cadherin release from coinfected tissues (Figure (Figure77B and Supplementary Figure 4). Taken together, these results confirmed that calpain activity is involved in C. albicans mucosal invasion during coinfection.

Figure 7.
Candida albicans mucosal invasion during coinfection is enhanced by calpain 1 and inhibited by calpeptin. A, Overlay images of mucosal analogue tissue sections stained with anti-Candida antibody (green), counterstained with nucleic acid stain Hoechst ...

DISCUSSION

Our results demonstrate that when C. albicans and S. oralis cocolonize the oral mucosa in high numbers they exploit epithelial calpain activity to compromise the integrity of the mucosal barrier. Although this is a common mechanism of epithelial barrier breach among certain pathogenic bacteria and parasites [3134], this is the first report of interkingdom synergy between 2 commensal organisms, which can promote invasive, opportunistic infection.

C. albicans invades oral epithelial cells by 2 major mechanisms: induced endocytosis and active penetration [5, 8, 19]. Active penetration requires fungal viability and includes penetration into or between epithelial cells [5, 7, 19]. In an oral carcinoma culture, active penetration between cells was shown to be a rare event [8, 19]. In contrast, in stratified organotypic cultures, which more faithfully mimic the oral mucosa, both processes can occur: penetration into superficial, nonkeratinized epithelial cells and between cells in the deeper epithelial strata [7, 22, 24 and current study]. In monoinfected mucosal analogues approximately 80% of C. albicans cells invaded the deeper strata paracellularly, compared with 95% of cells forming biofilm communities with S. oralis. The latter is a result of E-cadherin dissolution mediated by both fungal proteases [7] and host calpain activity, as shown in this study. Although it is hard to quantify the relative contribution of host and fungal proteases in this process in vivo, based on our experimental evidence, both mechanisms are likely to play a role.

Calpains are cysteine proteases with activities strictly regulated by calcium under physiological conditions [27]. Thus, it is plausible that costimulation with C. albicans and S. oralis increases intracellular calcium flux [15], which enhances translocation of calpain to cell membranes [31]. Toll-like receptor 2 (TLR2) signaling can regulate calcium flux to control calpain activity [15, 26]. Our earlier studies showed that TLR2 expression is significantly up-regulated in the oral mucosa of mice coinfected with C. albicans and S. oralis [14]; thus, it is plausible that TLR2 signaling is involved in calpain activation during coinfection.

S. oralis coaggregation receptor polysaccharides (RPS), adhesin receptors for biofilm organisms, trigger TLR2 expression in endothelial cells. However high RPS concentrations are needed to trigger these responses [35]. S. oralis does not form robust oral mucosal biofilms unless C. albicans is present [13, 14, 24]. It is thus possible that by promoting S. oralis biofilm growth C. albicans increases the availability of RPS for epithelial TLR2 signaling that also requires Candida costimulation. RPS exist on most strains of S. oralis (including strain 34 used in most of our experiments [36]), but only on certain strains of S. gordonii and S. mitis [37]. In addition, there are structural differences among the 3 oral streptococcal species in the RPS disaccharide motifs responsible for biological activity [37], which may explain species-specific epithelial responses.

In conclusion we showed that a fungal and a bacterial member of the core human oral microbiota can synergize to compromise the integrity of the oral mucosal barrier by activating epithelial calpain 1. Candida can take advantage of the disassembly of intercellular junctions to invade deeper into the oroesophageal mucosa and disseminate systemically. Although this route of dissemination was shown to occur in immunocompromised mice, it is still unknown whether this process occurs in humans.

Supplementary Data

Supplementary materials are available at http://jid.oxfordjournals.org. Consisting of data provided by the author to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the author, so questions or comments should be addressed to the author.

Supplementary Data:

Notes

Acknowledgments. Thanks to the following for their kind gifts: D. Sanglard for Candida albicans Δsap2456 (M31) and reference strain CAI4-URA3, P. E. Kolenbrander for Streptococcus oralis 34, L. Tao for S. oralis 108, and J. Tanzer for Streptococcus gordonii Challis CH1.

Financial support. This work was supported by the US Public Health Service through the National Institute of Dental and Craniofacial Research (grant RO1 DE013986).

Potential conflicts of interest. All authors: No potential conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

References

1. Ghannoum MA, Jurevic RJ, Mukherjee PK et al. Characterization of the oral fungal microbiome (mycobiome) in healthy individuals. Plos Pathog 2010; 6:e1000713. [PMC free article] [PubMed]
2. Dupuy AK, David MS, Li L et al. Redefining the human oral mycobiome with improved practices in amplicon-based taxonomy: discovery of Malassezia as a prominent commensal. Plos One 2014; 9:e90899. [PMC free article] [PubMed]
3. Rautemaa R, Ramage G Oral candidosis—clinical challenges of a biofilm disease. Crit Rev Microbiol 2011; 37:328–36. [PubMed]
4. Mavor AL, Thewes S, Hube B Systemic fungal infections caused by Candida species: epidemiology, infection process and virulence attributes. Curr Drug Targets 2005; 6:863–74. [PubMed]
5. Zhu W, Filler SG Interactions of Candida albicans with epithelial cells. Cell Microbiol 2010; 12:273–82. [PMC free article] [PubMed]
6. Park H, Myers CL, Sheppard DC et al. Role of the fungal Ras-protein kinase A pathway in governing epithelial cell interactions during oropharyngeal candidiasis. Cell Microbiol 2005; 7:499–510. [PubMed]
7. Villar CC, Kashleva H, Nobile CJ, Mitchell AP, Dongari-Bagtzoglou A Mucosal tissue invasion by Candida albicans is associated with E-cadherin degradation, mediated by transcription factor Rim101p and protease Sap5p. Infect Immun 2007; 75:2126–35. [PMC free article] [PubMed]
8. Wachtler B, Citiulo F, Jablonowski N et al. Candida albicans-epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process. Plos One 2012; 7:e36952. [PMC free article] [PubMed]
9. Frank CF, Hostetter MK Cleavage of E-cadherin: a mechanism for disruption of the intestinal epithelial barrier by Candida albicans. Transl Res 2007; 149:211–22. [PubMed]
10. Dongari-Bagtzoglou A, Kashleva H, Dwivedi P, Diaz P, Vasilakos J Characterization of mucosal Candida albicans Biofilms. Plos One 2009; 4:e7967. [PMC free article] [PubMed]
11. Xu H, Jenkinson HF, Dongari-Bagtzoglou A Innocent until proven guilty: mechanisms and roles of Streptococcus-Candida interactions in oral health and disease. Mol Oral Microbiol 2014; 29:99–116. [PMC free article] [PubMed]
12. Falsetta ML, Klein MI, Colonne PM et al. Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo. Infect Immun 2014; 82:1968–81. [PMC free article] [PubMed]
13. Diaz PI, Xie Z, Sobue T et al. Synergistic interaction between Candida albicans and commensal oral streptococci in a novel in vitro mucosal model. Infect Immun 2012; 80:620–32. [PMC free article] [PubMed]
14. Xu H, Sobue T, Thompson A et al. Streptococcal co-infection augments Candida pathogenicity by amplifying the mucosal inflammatory response. Cell Microbiol 2014; 16:214–31. [PMC free article] [PubMed]
15. Chun J, Prince A TLR2-induced calpain cleavage of epithelial junctional proteins facilitates leukocyte transmigration. Cell Host Microbe 2009; 5:47–58. [PMC free article] [PubMed]
16. Moretti D, Del Bello B, Allavena G, Maellaro E Calpains and cancer: friends or enemies? Arch Biochem Biophys 2014; 564:26–36. [PubMed]
17. Gillum AM, Tsay EY, Kirsch DR Isolation of the Candida albicans gene for orotidine-5’-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet 1984; 198:179–82. [PubMed]
18. Sanglard D, Hube B, Monod M, Odds FC, Gow NA A triple deletion of the secreted aspartyl proteinase genes SAP4, SAP5, and SAP6 of Candida albicans causes attenuated virulence. Infect Immun 1997; 65:3539–46. [PMC free article] [PubMed]
19. Dalle F, Wachtler B, L'Ollivier C et al. Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes. Cell Microbiol 2010; 12:248–71. [PubMed]
20. Wang X, Spandidos A, Wang H, Seed B PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update. Nucleic Acids Res 2012; 40:D1144–9. [PMC free article] [PubMed]
21. Spandidos A, Wang X, Wang H, Seed B PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 2010; 38:D792–9. [PMC free article] [PubMed]
22. Dongari-Bagtzoglou A, Kashleva H Development of a highly reproducible three-dimensional organotypic model of the oral mucosa. Nat Protoc 2006; 1:2012–8. [PMC free article] [PubMed]
23. Kankaanpaa P, Paavolainen L, Tiitta S et al. BioImageXD: an open, general-purpose and high-throughput image-processing platform. Nat Methods 2012; 9:683–9. [PubMed]
24. Bertolini MM, Xu H, Sobue T, Nobile CJ, Cury A, Dongari-Bagtzoglou A Candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes. Mol Oral Microbiol 2015; 30:307–22. [PMC free article] [PubMed]
25. Rollenhagen C, Wollert T, Langford GM, Sundstrom P Stimulation of cell motility and expression of late markers of differentiation in human oral keratinocytes by Candida albicans. Cell Microbiol 2009; 11:946–66. [PubMed]
26. O'Connor PM, Lapointe TK, Jackson S, Beck PL, Jones NL, Buret AG Helicobacter pylori activates calpain via Toll-like receptor 2 to disrupt adherens junctions in human gastric epithelial cells. Infect Immun 2011; 79:3887–94. [PMC free article] [PubMed]
27. Liu J, Liu MC, Wang KK Calpain in the CNS: from synaptic function to neurotoxicity. Sci Signal 2008; 1:re1. [PubMed]
28. Sloane JA, Hinman JD, Lubonia M, Hollander W, Abraham CR Age-dependent myelin degeneration and proteolysis of oligodendrocyte proteins is associated with the activation of calpain-1 in the rhesus monkey. J Neurochem 2003; 84:157–68. [PubMed]
29. Kumar R, Saraswat D, Tati S, Edgerton M Novel aggregation properties of Candida albicans secreted aspartyl proteinase Sap6 mediate virulence in oral candidiasis. Infect Immun 2015; 83:2614–26. [PMC free article] [PubMed]
30. Rios-Doria J, Day KC, Kuefer R et al. The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells. J Biol Chem 2003; 278:1372–9. [PubMed]
31. Sumitomo T, Nakata M, Higashino M et al. Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier. J Biol Chem 2011; 286:2750–61. [PMC free article] [PubMed]
32. Soong G, Martin FJ, Chun J, Cohen TS, Ahn DS, Prince A Staphylococcus aureus protein A mediates invasion across airway epithelial cells through activation of RhoA GTPase signaling and proteolytic activity. J Biol Chem 2011; 286:35891–8. [PMC free article] [PubMed]
33. Perez-Cordon G, Nie W, Schmidt D, Tzipori S, Feng H Involvement of host calpain in the invasion of Cryptosporidium parvum. Microbes Infect 2011; 13:103–7. [PMC free article] [PubMed]
34. Tenenbaum T, Matalon D, Adam R et al. Dexamethasone prevents alteration of tight junction-associated proteins and barrier function in porcine choroid plexus epithelial cells after infection with Streptococcus suis in vitro. Brain Res 2008; 1229:1–17. [PubMed]
35. de Toledo A, Nagata E, Yoshida Y, Oho T Streptococcus oralis coaggregation receptor polysaccharides induce inflammatory responses in human aortic endothelial cells. Mol Oral Microbiol 2012; 27:295–307. [PubMed]
36. Cisar J, Sandberg A, Reddy P, Abeygunawardana C, Bush C Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral Actinomyces and streptococcal lectins. Infect Immun 1997; 65:5035–41. [PMC free article] [PubMed]
37. Yoshida Y, Yang J, Nagano K, Yoshimura F, Cisar J Cell surface coaggregation receptor polysaccharide of oral streptococci. J Oral Biosci 2014; 56:125–30.

Articles from The Journal of Infectious Diseases are provided here courtesy of Oxford University Press