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A visible-light (470 nm wavelength) sensitive Type II photoinitiator system is developed for bulk Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions in crosslinked networks. The accelerated photopolymerization eliminates UV-mediated azide decomposition allowing for the formation of defect-free glassy networks which exhibit a narrow glass transition temperature.
Bulk photo-CuAAC crosslinked networks are rapidly polymerized using blue light (470 nm) sensitive novel photoinitiator system mitigating defects from azide decomposition. The high modulus network films reach high conversion under short irradiation time with narrow glass transition region.
The Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) reaction is one of the most widely implemented ‘click’ reactions1. The wide range of conditions in which the bio-orthogonal CuAAC reaction can be applied makes it immensely useful for an array of chemical2 and material3–4 research application such as hydrogels5–7, bioconjugation8–9, surface modifications10–11, and dendrimers12–13. Typically, the reaction proceeds through the in situ generation of catalytic Cu(I) from Cu(II) using a reducing agent such as sodium ascorbate14–16. In the last decade, several researchers have also generated Cu(I) using a photo-chemical route for a range of small molecule17–18 and polymerization19 applications. In the photo-CuAAC reaction, copper (II) is reduced through a reaction with a radical species produced via irradiation of either a Norrish Type I (α-cleavage) or Norrish Type II (electron transfer) photoinitiator20–22. The generation of the Cu(I) catalyst using light enables temporal control; moreover, despite the potential of Cu(I) to diffuse through the system the spatial control is also observed likely owing to Cu(I) interactions with the triazole product19, 23.
Several favourable attributes of the photo-CuAAC reaction in neat (solventless) polymerizations of multifunctional azide and alkyne monomers are diminished by the formation of defects (bubbles). These defects formed upon polymerization under UV light compromise the structural integrity of the film. It is well recognized in the literature that the azide functional group exhibits susceptibility to photodecomposition, particularly under ultraviolet (UV) light exposure, forming a reactive nitrene species and emitting nitrogen gas24–27. For example, aromatic azides are used in light-initiated azide-amine bioconjugation reactions, where azides photodecompose to form a reactive nitrene followed by ring expansion and reaction with the amine substrate28–29. While the observation of bubble formation is notably absent in the literature, we herein report that bubbles do indeed form during the photopolymerization of a neat (solventless) crosslinked polymer. The formation and extent of bubbles depend on a number of experimental conditions, including the wavelength and intensity of light, sample thickness, and roughness of the sample preparing surface (presence of nucleation sites); however, the generation of nitrogen bubbles can be prevented entirely through the use of longer wavelength light in conjunction with an appropriately absorbance-matched photoinitiating system.
Inspired by the widely used blue-light activated methacrylate-based dental restorative resin system30–31, we introduce an accelerated photo-CuAAC bulk polymerization system to obtain glassy crosslinked networks that are defect (bubble) free. The developed blue-light sensitive Type II photosensitizing system includes optimized concentrations of camphorquinone and a hydrogen-donating tertiary amine as an accelerator to afford polymer networks that are not only completely defect-free under all experimental conditions but also show rapid polymerization. This methodology to obtain bulk photo-CuAAC network additionally takes advantage of ‘dark polymerization’, wherein the network continues to crosslink after irradiation is ceased, owing to the persistence of catalytic Cu(I) species32–33. The photo-CuAAC-based polymerization maintains a step-growth molecular weight evolution producing characteristic high functional group conversions with a narrow glass transition region.
Typically, bulk photopolymerized CuAAC network systems have been initiated with UV wavelength light (365 nm)34 or near-UV wavelengths (405 nm)35 using an appropriate absorbance-matched photoinitiator species. As discussed, continuous UV irradiation at these wavelengths can lead to the photo-decomposition of azide functional groups present on the monomers. While azide photodecomposition is a side reaction compared to the azide-alkyne crosslinking reaction, bubbles are clearly observed in 0.5 mm thick films formed using monomers 1 and 2 (see Fig. 1 A). Correspondingly, the CuAAC resins irradiated with near UV i.e. 405 nm wavelength light contain qualitatively fewer bubbles but still compromising the specimen.
For samples irradiated with 365 and 405 nm light, we have noted several experimental conditions that affect bubble formation in the photo-CuAAC network system shown in Fig. 1 B. First, increasing light intensity and dose, which is necessary to have complete conversion in the depth of thick samples, increases the amount and size of bubbles in the system. Specifically, fewer bubbles were observed in thinner samples irradiated over a smaller timescale. Second, the surface roughness on which the CuAAC reaction is performed appears to affect bubble formation, presumably by providing additional nucleation sites. Nevertheless, the wavelength of light remains the main experimental condition that controls bubble formation (i.e., shorter wavelengths lead to increased bubble formation).
To support our hypothesis that azide photo-decomposition is the source of the observed bubble defects, UV-Vis spectroscopy of the azide monomer (monomer 1) (1mM in N,N-Dimethylformamide) was performed under irradiation with UV-light (365 nm) and blue-light (470 nm) at an intensity of 40mW/cm2. Fig. 2 A. shows the decrease in absorbance in the peak associated with azide at 290 nm for only monomer 1 in DMF irradiated using 365 nm UV light. The nitrogen gas produced was further verified using gas chromatography (GC) mass spectroscopy (see Figure S1 in ESI†). In contrast, the azide absorbance remains unchanged under 470nm blue-light confirming the stability of azide monomer under longer wavelength of similar intensity.
Wavelength selection is hence critical to avoid bubble formation during bulk photopolymerization of an azide and alkyne based resin. Camphorquinone (5; CQ), a photosensitizing cyclic ketone, is a well-known visible light photoinitiator with peak absorption of 469 nm36 and is used in photopolymerization of methacrylate monomer-based dental composites37. CQ alone has been shown to photoinitiate CuAAC using a mercury lamp equipped with a 400–500 nm band filter for small molecules by Tasdelen et al.38 and in bulk polymerization by Song et al.33 at moderately elevated temperatures (35 °C). Under blue-light during photopolymerization of vinyl monomers, the use of a co-initiator amine species in conjunction with CQ has been utilized as a kinetic accelerator39. Motivated by this approach, we developed a novel CQ-amine photoinitiation system for accelerated bulk photo-CuAAC polymerization networks.
The CQ-tertiary amine is a Norrish Type II photoinitiation system and follows a well-known two stage initiation process. Briefly, 470nm wavelength light excites the CQ to a singlet state during the n-σ* transition. After an inter-system crossing (ISC) from the singlet to the triplet state, CQ interacts via electron transfer with the tertiary amine to form an excited complex (exciplex), which then extracts hydrogen from the amine to form a radical on the α-C atom of the tertiary amine40–41. The stability of this radical is critical in the H transfer process37, 39, 42. Unique to the photo-CuAAC reaction scheme, the radical on the amine then reduces Cu(II) to Cu(I) which in turn catalyzes the CuAAC reaction portrayed in Fig. 3 A.
The accelerated photopolymerization kinetics were monitored in real-time by tracking decrease in the area of the alkyne peak (6540-6460 cm−1) using near-IR (NIR) spectroscopy while simultaneously irradiating the sample (Fig. 3 B). The inclusion of a tertiary amine in the CuAAC formulation yields a rapid alkyne functional group conversion of ≈80% after 15 minutes. The amine was added to monomers in 1:1 CQ:amine ratio (w/w) (i.e., 0.35 wt% of 5 and 0.35 wt% of 6 of the total monomer weight in the CuAAC formulation) and polymerized under 470 nm filtered light of intensity 40 mW cm−2 to yield a bubble free network. The formulations not containing the accelerating amine exhibit only 5% conversion at equal irradiation intensity and time; it is possible that the excited CQ could abstract an H-atom from the PMDETA (Cu-ligand)18, 43 or from the urethane group on monomer 133, 44. The control sample with no CQ and no amine shows negligible conversion throughout. The CQ:amine ratio used is optimized at various light intensities to ensure minimum leachable amine in the glassy network (see Figure S2 in ESI†). The effect of light intensity, relative amounts of CQ and amine, and kinetics of three different amines structure are also studied in detail (see Figure S3 – S5 in ESI†).
One of the main attributes of photo-CuAAC reactions is their ability to continue reaction after the initiating light has ceased (i.e., dark-polymerization)19, 32–33. In Fig. 4. with controlled time of illumination (5, 10, 15, and 40 minutes); the conversion of the polymerization is tracked. The rate and extent of the dark polymerization depends on initial time of visible light illumination converting the corresponding amount of Cu(II) to Cu(I). The kinetics of the polymerization are similar at all irradiation times, depicting its efficiency in forming a stable Cu(I) catalyst from the Type II photoinitiation system and retaining the dark polymerization behaviour that is characteristic of photo-CuAAC systems. Not only can the sample be irradiated at short time intervals while maintaining similar kinetic results, but the overall reaction also reaches high conversions in a short amount of time (>75% conversion in less than 10 minutes). The dark polymerization behaviour is seen with different amine structures in photo-CuAAC networks and upon tracking the reaction for 12 hours, the networks reaches about 98% conversion due to the stability of the Cu(I) catalyst in the network (see Figure S6 in ESI†).
The mechanical properties of the glassy, defect-free photo-CuAAC network sample (image in Fig. 5 A), photopolymerized under ambient conditions are of prime importance. These properties are analysed using a Dynamic Mechanical Analyser (DMA) (shown in Fig. 5 B). The narrow Tan Delta peak indicates the homogenous crosslinking structure formed during the step growth polymerization. The glass transition temperature (Tg) of 67 °C and the storage modulus of 2.2 GPa at room temperature makes photo-CuAAC conducive for glassy film applications. Furthermore, it is consistent with reported bulk photo-CuAAC network systems from the same monomers with other photoinitiation systems45.
In conclusion, a novel 470 nm (blue-light) initiation system is developed for photo-CuAAC bulk polymerizations. This readily integrable new method provide significant advantages over previous systems. First, azide decomposition is prevented and hence the polymer networks are defect-free. Second, the kinetics of photo-CuAAC are accelerated (>75% conversion in 10 minutes) at room temperature and optimized for a Type II photoinitiator system using camphorquinone in conjunction with a tertiary amine. Third, the network retains the characteristics that are desirable with photo-CuAAC systems, including dark polymerization after photoinitiation is ceased, high moduli, and narrow glass transition temperatures.
We acknowledge the financial support from NIH-NIDCR (U01 DE023774) and NIH-COBRE (1P30 GM110758). We thank Marco Dunwell, Jeffrey Heyes, and Prof. Bingjun Xu for help with gas-chromatography instrument and Dr. Chen Guo, Prof. April Kloxin for help with UV-Vis spectroscopy instrument.
†Electronic Supplementary Information (ESI) available: Gas-chromatography analysis of defect from monomer 1 under irradiation. The effect on kinetics from photo-initiator concentration optimization, light intensity, three different amine structure and overnight conversion showing dark polymerization.