PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jclinpathJournal of Clinical PathologyVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
 
J Clin Pathol. 1983 December; 36(12): 1357–1361.
PMCID: PMC498568

Selective Kirchner medium in the culture of specimens other than sputum for mycobacteria.

Abstract

Over a two-year period, 2949 non-sputum specimens were cultured on two slopes of Löwenstein-Jensen (LJ) medium, two bottles of Kirchner liquid medium, made selective by adding polymyxin B, carbenicillin, trimethoprim and amphotericin B, and a selective 7H11 agar slope. Pus and CSF were inoculated into this set without prior treatment, but other specimens were decontaminated with sulphuric acid. Tissues and fluids were also inoculated without decontamination into additional selective media. The use of the selective media as well as the LJ slopes increased the yield of specimens with cultures of tubercle bacilli from 34 to 53 and decreased specimens with all media contaminated from 34 to 2. Results almost as good, 52 positive specimens and five totally contaminated, would have been obtained by the addition of a single selective Kirchner bottle to the two LJ slopes, and this is recommended for routine use.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (723K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allen BW, Darrell JH. Extrapulmonary tuberculosis: a potential source of laboratory-acquired infection. J Clin Pathol. 1981 Apr;34(4):404–407. [PMC free article] [PubMed]
  • Marks J. Ending the routine guinea-pig test. Tubercle. 1972 Mar;53(1):31–34. [PubMed]
  • Mitchison DA, Allen BW, Lambert RA. Selective media in the isolation of tubercle bacilli from tissues. J Clin Pathol. 1973 Apr;26(4):250–252. [PMC free article] [PubMed]
  • Mitchison DA, Allen BW, Carrol L, Dickinson JM, Aber VR. A selective oleic acid albumin agar medium for tubercle bacilli. J Med Microbiol. 1972 May;5(2):165–175. [PubMed]
  • Mitchison A, Aber VR. Culture of specimens other than sputum for Mycobacteria. J Clin Pathol. 1974 Nov;27(11):883–887. [PMC free article] [PubMed]
  • Allen BW, Mitchison DA, Darbyshire J, Chew WW, Gabriel M. Examination of operation specimens from patients with spinal tuberculosis for tubercle bacilli. J Clin Pathol. 1983 Jun;36(6):662–666. [PMC free article] [PubMed]
  • Canetti G, Fox W, Khomenko A, Mahler HT, Menon NK, Mitchison DA, Rist N, Smelev NA. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ. 1969;41(1):21–43. [PubMed]
  • McClatchy JK, Waggoner RF, Kanes W, Cernich MS, Bolton TL. Isolation of mycobacteria from clinical specimens by use of selective 7H11 medium. Am J Clin Pathol. 1976 Mar;65(3):412–415. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Group