Search tips
Search criteria 


Logo of jclinpathJournal of Clinical PathologyVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
J Clin Pathol. 1992 August; 45(8): 684–688.
PMCID: PMC495144

Adenovirus infection of the large bowel in HIV positive patients.


AIMS: To describe the microscopic appearance of adenovirus infection in the large bowel of human immunodeficiency virus (HIV) positive patients with diarrhoea. METHODS: Large bowel biopsy specimens from 10 HIV positive patients, eight of whom were also infected with other gastrointestinal pathogens, with diarrhoea were examined, together with six small bowel biopsy specimens from the same group of patients. Eight of the patients had AIDS. The biopsy specimens were examined by light microscopy performed on haematoxylin and eosin stained and immunoperoxidase preparations, the latter using a commercially available antibody (Serotec MCA 489). Confirmation was obtained with electron microscopy. RESULTS: The morphological appearance of cells infected with adenovirus showed characteristic nuclear and cellular changes, although the inflammatory reaction was non-specific. Immunoperoxidase staining for adenovirus was sensitive and specific, and the presence of viral inclusions consistent with adenovirus was confirmed by electron microscopy. CONCLUSIONS: The light microscopic features of adenovirus infection are distinctive and immunocytochemistry with a commercially available antibody is a sensitive and specific means of confirming the diagnosis. Further studies of the role of adenovirus in causing diarrhoea in these patients are indicated.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Francis ND, Boylston AW, Roberts AH, Parkin JM, Pinching AJ. Cytomegalovirus infection in gastrointestinal tracts of patients infected with HIV-1 or AIDS. J Clin Pathol. 1989 Oct;42(10):1055–1064. [PMC free article] [PubMed]
  • Janoff EN, Orenstein JM, Manischewitz JF, Smith PD. Adenovirus colitis in the acquired immunodeficiency syndrome. Gastroenterology. 1991 Apr;100(4):976–979. [PubMed]
  • Krilov LR, Rubin LG, Frogel M, Gloster E, Ni K, Kaplan M, Lipson SM. Disseminated adenovirus infection with hepatic necrosis in patients with human immunodeficiency virus infection and other immunodeficiency states. Rev Infect Dis. 1990 Mar-Apr;12(2):303–307. [PubMed]
  • Varki NM, Bhuta S, Drake T, Porter DD. Adenovirus hepatitis in two successive liver transplants in a child. Arch Pathol Lab Med. 1990 Jan;114(1):106–109. [PubMed]
  • Landry ML, Fong CK, Neddermann K, Solomon L, Hsiung GD. Disseminated adenovirus infection in an immunocompromised host. Pitfalls in diagnosis. Am J Med. 1987 Sep;83(3):555–559. [PubMed]
  • Uhnoo I, Wadell G, Svensson L, Johansson ME. Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol. 1984 Sep;20(3):365–372. [PMC free article] [PubMed]
  • Gottlieb MS, Schroff R, Schanker HM, Weisman JD, Fan PT, Wolf RA, Saxon A. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med. 1981 Dec 10;305(24):1425–1431. [PubMed]
  • Siegal FP, Lopez C, Hammer GS, Brown AE, Kornfeld SJ, Gold J, Hassett J, Hirschman SZ, Cunningham-Rundles C, Adelsberg BR, et al. Severe acquired immunodeficiency in male homosexuals, manifested by chronic perianal ulcerative herpes simplex lesions. N Engl J Med. 1981 Dec 10;305(24):1439–1444. [PubMed]
  • de Jong PJ, Valderrama G, Spigland I, Horwitz MS. Adenovirus isolates from urine of patients with acquired immunodeficiency syndrome. Lancet. 1983 Jun 11;1(8337):1293–1296. [PubMed]
  • Hierholzer JC, Wigand R, Anderson LJ, Adrian T, Gold JW. Adenoviruses from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (types 43-47). J Infect Dis. 1988 Oct;158(4):804–813. [PubMed]
  • Connolly GM, Shanson D, Hawkins DA, Webster JN, Gazzard BG. Non-cryptosporidial diarrhoea in human immunodeficiency virus (HIV) infected patients. Gut. 1989 Feb;30(2):195–200. [PMC free article] [PubMed]
  • Connolly GM, Dryden MS, Shanson DC, Gazzard BG. Cryptosporidial diarrhoea in AIDS and its treatment. Gut. 1988 May;29(5):593–597. [PMC free article] [PubMed]
  • Rolston KV, Rodriguez S, Hernandez M, Bodey GP. Diarrhea in patients infected with the human immunodeficiency virus. Am J Med. 1989 Jan;86(1):137–138. [PubMed]
  • Laughon BE, Druckman DA, Vernon A, Quinn TC, Polk BF, Modlin JF, Yolken RH, Bartlett JG. Prevalence of enteric pathogens in homosexual men with and without acquired immunodeficiency syndrome. Gastroenterology. 1988 Apr;94(4):984–993. [PubMed]
  • Kaljot KT, Ling JP, Gold JW, Laughon BE, Bartlett JG, Kotler DP, Oshiro LS, Greenberg HB. Prevalence of acute enteric viral pathogens in acquired immunodeficiency syndrome patients with diarrhea. Gastroenterology. 1989 Oct;97(4):1031–1032. [PubMed]
  • Cunningham AL, Grohman GS, Harkness J, Law C, Marriott D, Tindall B, Cooper DA. Gastrointestinal viral infections in homosexual men who were symptomatic and seropositive for human immunodeficiency virus. J Infect Dis. 1988 Aug;158(2):386–391. [PubMed]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group