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Br J Pharmacol. 2016 April; 173(7): 1208–1218.
Published online 2016 March 1. doi:  10.1111/bph.13420
PMCID: PMC4941126

Eact, a small molecule activator of TMEM16A, activates TRPV1 and elicits pain‐ and itch‐related behaviours

Abstract

Background and Purpose

TMEM16A, also known as anoctamin 1 channel, is a member of the Ca2 +‐activated chloride channels family and serves as a heat sensor in the primary nociceptors. Eact is a recently discovered small molecule activator of the TMEM16A channel. Here, we asked if Eact produces pain‐ and itch‐related responses in vivo and investigated the cellular and molecular basis of Eact‐elicited responses in dorsal root ganglia (DRG) neurons.

Experimental Approach

We employed behavioural testing combined with pharmacological inhibition and genetic ablation approaches to identify transient receptor potential vanilloid 1 (TRPV1) as the prominent mediator for Eact‐evoked itch‐ or pain‐related responses. We investigated the effects of Eact on TRPV1 and TMEM16A channels expressed in HEK293T cells and in DRG neurons isolated from wild type and Trpv1 / mice using Ca2 + imaging and patch‐clamp recordings. We also used site‐directed mutagenesis to determine the molecular basis of Eact activation of TRPV1.

Key Results

Administration of Eact elicited both itch‐ and pain‐related behaviours. Unexpectedly, the Eact‐elicited behavioural responses were dependent on the function of TRPV1, as shown by pharmacological inhibition and genetic ablation studies. Eact activated membrane currents and increased intracellular free Ca2 + in both TRPV1‐expressing HEK293T cells and isolated DRG neurons in a TRPV1‐dependent manner. Eact activation of the TRPV1 channel was severely attenuated by mutations disrupting the capsaicin‐binding sites.

Conclusions and Implications

Our results suggest that Eact activates primary sensory nociceptors and produces both pain and itch responses mainly through direct activation of TRPV1 channels.

Abbreviations

DRG
dorsal root ganglia
Eact
[3,4,5‐trimethoxy‐N‐(2‐methoxyethyl)‐N‐(4‐phenyl‐2‐thiazolyl)benzamide]
TG
trigeminal ganglion
WT
wild type

Table of Links

TARGETS
Voltage‐gated ion channels a Other ion channels b
TRPA1 TMEM16A
TRPM8 Enzymes c
TRPV1 Collagenase 2 (MMP8)
TRPV4 Papain

These Tables list key protein targets and ligands in this article which are hyperlinked to corresponding entries in http://www.guidetopharmacology.org, the common portal for data from the IUPHAR/BPS Guide to PHARMACOLOGY (Pawson et al., 2014) and are permanently archived in the Concise Guide to PHARMACOLOGY 2015/16 (a,b,cAlexander et al., 2015a, 2015b, 2015c).

Introduction

The transmembrane protein 16 A (TMEM16A) channel, also known as anoctamin 1, is a recently identified Ca2 +‐activated chloride channel (CaCC) with eight transmembrane domains (Schroeder et al., 2008). TMEM16A has many important physiological functions, for example, it regulates chloride secretion from exocrine glands and epithelial cells, smooth muscle contraction and pace making activity of interstitial cells of Cajal in the gut (Huang et al., 2009; Iqbal et al., 2012; Mroz and Keely, 2012; Singh et al., 2014). TMEM16A also regulates the excitability of many types of neurons, including neurons in the spiral ganglion (Zhang et al., 2015), trigeminal ganglion (TG) (Kanazawa and Matsumoto, 2014) and dorsal root ganglion (DRG) (Cho et al., 2012), in which TMEM16A serves as a molecular sensor detecting noxious thermal stimuli.

Given the importance of TMEM16A in numerous physiological and pathological conditions, much attention has recently been devoted to the development of pharmacological agents with either agonistic or antagonistic action on TMEM16A. Identification of selective TMEM16A activators is not only critical to dissecting the physiological function of TMEM16A in native tissues and studying biophysical properties of TMEM16A, but also potentially for providing a novel therapeutic intervention for cystic fibrosis and other related diseases by stimulating TMEM16A ‐mediated chloride secretion. Eact is one of the small molecule TMEM16A activators that was discovered by a cell‐based high throughput screening of ≈110 000 compounds (Namkung et al., 2011). Consistent with it being a potent TMEM16A activator, Eact directly opens TMEM16A without increasing intracellular Ca2 + ([Ca2 +]i), and stimulates chloride secretion from human airway epithelial cells and submucosal gland (Namkung et al., 2011). Eact also causes contraction in mouse intestinal strips, activates chloride current in rat arterial myocytes (Burris et al., 2015) and inhibits oestradiol production in cultured mouse granulosa cells (Sun et al., 2014).

The transient receptor potential vanilloid 1 (TRPV1) channel is a major marker of nociceptive primary sensory neurons in the DRG, TG and vagal sensory ganglia (Ahern, 2003; Caterina et al., 1999; Lukacs et al., 2013). TRPV1 is activated by noxious heat, tissue acidosis, endogenous ligands such as anandamide and numerous exogenous compounds including capsaicin (Ross, 2003; Tominaga et al., 1998; Watanabe et al., 2003). TRPV1 also plays a crucial role in the development and maintenance of thermal hyperlagesia elicited by tissue inflammation or nerve injury (Caterina et al., 2000; Davis et al., 2000). More recently, TRPV1 was shown to mediate the histamine‐dependent scratching response (Imamachi et al., 2009).

Interestingly, both TMEM16A and TRPV1 are not only involved in detecting thermal pain, but are also highly co‐localized in a subset of small‐diameter DRG neurons (Cho et al., 2012), suggesting the potential for functional coupling between these two channels. Therefore, pharmacological activation or inhibition of TMEM16A might affect the functions of TRPV1‐expressing neurons. Moreover, in the present study, we found that Eact unexpectedly activated TRPV1 channels through a direct interaction with the capsaicin binding site. In vivo, Eact was found to produce both itch‐ and pain‐related behaviours in a TRPV1‐dependent manner.

Methods

Animals

Animal studies are reported in compliance with the ARRIVE guidelines (Kilkenny et al., 2010; McGrath and Lilley, 2015). Both wild type (WT) and congenic Trpv1 / mice on the C57BL/6 J background were obtained from Jackson Laboratories. All mice were housed under a 12 h light/dark cycle with food and water provided ad libitum. All experiments were performed in accordance with the guidelines of the National Institutes of Health and the International Association for the Study of Pain, and were approved by the Animal Studies Committee at Washington University School of Medicine.

HEK293T cell culture and transfection

HEK293T cells were grown as a monolayer using passage numbers less than 30 and maintained in DMEM (Life Technologies, Carlsbad, CA, USA), supplemented with 10% FBS (Life Technologies), 100 U mL−1 penicillin, and 100 μg mL−1 streptomycin in a humidified incubator at 37°C with 5% CO2. The cells were transiently transfected with a cDNA for mouse TRPV1 (mTRPV1), individual mTRPV1 mutants, human TRPA1 (hTRPA1), hTRPM8, rat TRPV4 (rTRPV4) or mTMEM16A using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) with a ratio of 0.8:2. Following transfection, the cells were maintained in DMEM at 37°C for 24 h before use. All TRPV1 mutants were made using the QuikChange II XL Mutagenesis Kit (Agilent Technologies Inc. Santa Clara, CA, USA) according to the manufacturer's directions. All mutations were confirmed by DNA sequencing.

siRNA knockdown of TMEM16A

HEK293T cells plated in 48‐well plates were transfected with either 200 nM TMEM16A siRNA (HSS123904; 5′‐AAG UUA GUG AGG UAG GCU GGG AAC C‐3′, Life Technologies) or 200 nM scrambled siRNA control (12935300; 5′‐GGU UCC CAG CCU ACC UCA CUA ACU U‐3′, Life Technologies) using Lipofectamine 2000 (Life Technologies) at a 20:2 ratio (pmol siRNA: μL Lipofectamine 2000); mTRPV1 was co‐transfected with siRNA or scrambled siRNA control; 48 h later, cells were plated onto round coverslips and incubated for an additional 24 h. Knockdown of TMEM16A expression was assayed by Western blot.

Western blot

HEK293T cells were pelleted, lysed with cell lysis buffer (25 mM Tris–HCl, pH 7.6, 150 mM NaCl, 0.1% SDS, 1 mM each, PMSF, NaF, NaVO3, 1 μg mL−1 each, leupeptin, pepstatin, aprotinin), followed by centrifugation at 12 000 g for 20 min. The protein concentration of the supernatant was determined by BCA Protein Assay Kit (Pierce, Rockford, IL, USA); 120 μg protein was boiled for 3 min at 100°C with an appropriate volume of 5× SDS‐PAGE sample loading buffer (250 mM Tris–HCl, pH 6.8, 5% 2‐mercaptoethanol, 10% SDS, 0.5% bromophenol blue, 50% glycerol). Samples were loaded into each lane of a 12% SDS‐PAGE gel. The membrane was blocked by 5% BSA in TBS‐T (50 mM Tris–HCl, pH 7.6, 140 mM NaCl, 0.1% Tween 20) at 4°C overnight and then incubated with primary and secondary antibodies diluted in blocking solution at room temperature for 3 h. Blots were developed in ECL (Pierce, Rockford, IL, USA) solution for 3 min and exposed onto a Kodak X‐OMAT AR film (Eastman Kodak, Rochester, NY, USA) for 3 min. The antibodies used were rabbit anti‐TMEM16A (ab84115) (1:1000, Abcam, Cambridge, MA, USA), HRP‐anti‐rabbit secondary antibody (1:1000, Santa Cruz, CA, USA). Mouse HRP‐anti‐β‐actin (1:5000, Santa Cruz, CA, USA) was used as a loading control. Densitometry analysis of TMEM16A bands and β‐actin bands were performed using Image J software (GeneGnome, Syngene, Frederick, MD, USA). The same size square was drawn around each band to measure the density after the background near that band was subtracted. TMEM16A levels were normalized against β‐actin levels and expressed as fold increase.

Isolation and short‐term culture of mouse DRG neurons

Animals were anaesthetized with isoflurane followed by cervical dislocation. The spinal column was removed and placed in ice‐cold HBSS; laminectomies were performed to dissect out bilateral DRG. Neurons were acutely dissociated and maintained as described previously (Yin et al., 2013, 2014). In brief, after removal of connective tissues, DRG were transferred to 1 mL Ca2 +/Mg2 +‐free HBSS containing 2 μL saturated NaHCO3, 0.35 mg l‐cysteine, and 20 U papain (Worthington) and incubated at 37°C for 10 min. DRGs were spun down, the supernatant was removed and 1 mL Ca2 +/Mg2 +‐free HBSS containing 4 mg collagenase type II and 1.25 mg dispase type II (all from Sigma‐Aldrich) was added and incubated at 37°C for another 10 min. After digestion, neurons were pelleted, suspended in the neurobasal medium containing 2% B‐27 supplement, 1% l‐glutamine, 100 U mL−1 penicillin plus 100 μg mL−1 streptomycin, and 50 ng mL−1 nerve growth factor, plated on a 12 mm coverslip coated with poly‐l‐lysine (10 μg mL−1) and cultured under a humidified atmosphere of 5% CO2/95% air at 37°C for 18–24 h before use.

Live cell Ca2+ imaging

Cultured DRG neurons and HEK293T cells expressing TRP channels were loaded with 4 μM Fura‐2 AM (Life Technologies) in the culture medium at 37°C for 60 min. Cells were then washed three times and incubated in HBSS at room temperature for 30 min before use. Fluorescence at 340 and 380 nm excitation wavelengths was recorded on an inverted Nikon Ti‐E microscope equipped with 340, 360 and 380 nm excitation filter wheels using NIS‐elements imaging software (Nikon Instruments Inc., Melville, NY, USA). Fura‐2 ratios (F340/F380) reflect changes in [Ca2 +]i upon stimulation. Values were obtained from 100 to 250 cells in time‐lapse images from each coverslip. Threshold of activation was defined as 3 SDs above the average (~20% above the baseline).

Patch‐clamp recordings

Whole‐cell and single‐channel patch‐clamp recordings were performed using an Axon 700B amplifier (Molecular Devices, Sunnyvale, CA, USA) at room temperature (22–24°C) on the stage of an inverted phase‐contrast microscope equipped with a filter set for GFP visualization. Pipettes pulled from borosilicate glass (BF 150‐86‐10; Sutter Instrument, Novato, CA, USA) with a Sutter P‐1000 pipette puller had resistances of 2–4 and 8–10 MΩ for whole‐cell and single‐channel recordings, respectively, when filled with pipette solution containing 140 mM CsCl, 2 mM EGTA and 10 mM HEPES with pH 7.3 and 315 mOsm L−1 osmolarity. Symmetrical solutions with the same components as that in the pipette solution were used for single‐channel recordings. The Clfree pipette solution contained 140 mM caesium acetate, 2 mM EGTA and 10 mM HEPES with pH 7.3 and 315 mOsm L−1 osmolarity. A Ca2 +‐free extracellular solution was used for whole‐cell recording to avoid Ca2 +‐dependent desensitization of TRPV1; this solution contained 140 mM NaCl, 5 mM KCl, 0.5 mM EGTA, 1 mM MgCl2, 10 mM glucose and 10 mM HEPES (pH was adjusted to 7.4 with NaOH, and the osmolarity was adjusted to ≈340 mOsm L−1 with sucrose). The whole‐cell membrane currents were recorded using voltage ramps from −100 to +100 mV for 500 ms at a holding potential of 0 mV. Data were acquired using clampex 10.4 software (Molecular Devices). Currents were filtered at 2 kHz and digitized at 10 kHz. Data were analysed and plotted using Clampfit 10 (Molecular Devices). Single‐channel events were identified on the basis of the half‐amplitude threshold‐crossing criteria. Open probability was determined from idealized traces as the ratio of the sum of all open durations to the total trace duration using Clampfit 10.

Nocifensive response

Intraplantar injection of Eact was used to induce nociceptive responses as described previously (Caterina et al., 2000). Immediately after injection, mice were placed inside a Plexiglas chamber. Total time spent licking and lifting the injected hind paw was measured from video recordings (5 min). AMG9810 was administered i.p. 30 min before intraplantar injection of Eact. Control mice were injected with an equal volume of the vehicle (0.9% saline +1% DMSO +0.1% Tween 80).

Thermal pain test

Paw withdrawal latencies in response to radiant heat were measured using the Hargreaves apparatus (IITC Life Science Inc) (Caterina et al., 2000). Briefly, each mouse was placed individually in a clear Plexiglas chamber (8 × 8 × 12 cm) and acclimatized to it for at least 1 h before testing. Right hind paws of mice were injected intraplantarly with 20 μL vehicle (0.9% saline +1% DMSO +0.1% Tween 80) with or without chemicals. For assessment of thermal nociception, right hind paw withdrawal latencies were measured before (0 min) and 15, 30, 60, 90 and 120 min after injections. The infrared intensity was adjusted to obtain basal paw withdrawal latencies of 10 to 15 s. An automatic 20 s cut‐off was used to prevent tissue damage.

Scratching behaviour

Mouse scratching responses were measured as described previously (Sun and Chen, 2007; Sun et al., 2009). Briefly, mice were placed individually in transparent cages for at least 30 min before assays and received Eact or vehicle control by i.d. injections into the rostral back. The number of hind limb scratching bouts directed to the injection site over a 30 min period was calculated.

All mice were randomized to experimental groups. All behavioural tests were videotaped from a side angle, and behavioural assessments were carried out by observers blind to the treatments or genotypes of the animals.

Statistics

The data and statistical analysis comply with the recommendations on experimental design and analysis in pharmacology (Curtis et al., 2015).

The concentration‐response curve of Eact‐activated outward currents at +60 mV was fitted with the logistic equation: Y = Ymin + (Ymax − Ymin)/(1 + 10^[(logEC50 − X) × Hill slope]), where Y is the response at a given concentration, Ymax and Ymin are the maximum and minimum responses, X is the logarithmic value of the concentration and Hill slope is the slope factor of the curve. EC50 is the concentration that gives a response halfway between Ymax and Ymin. All data are presented as mean ± SEM for n independent observations. Student's t‐test was used to analyse statistical significance between two groups. anova and repeated measures tests were used to test hypotheses about effects in multiple groups occurring over time. P < 0.05 was considered significantly different.

Chemicals

Capsaicin, GSK1016790A, AMG9810 and T16Ainh‐A01 were purchased from Sigma‐Aldrich (St. Louis, MO, USA ); Eact was from Tocris (Ellisville, MO, USA); allyl isothiocyanate was from ACROS (Geel, Belgium); menthol was from MP Biomedicals (Santa Ana, CA, USA); papain and collagenase (type 2) were from Worthington (Lakewood, NJ, USA).

Results

Eact elicits both itch‐ and pain‐related behaviours in a TRPV1‐dependent manner

TMEM16A is activated by increased intracellular free Ca2 + ([Ca2 +]i) evoked by activation of many GPCRs including the histamine H1 receptor and endothelin ETA receptor, which are important itch mediators (Cho et al., 2012; Luo et al., 2015; Yang et al., 2008). Furthermore, TMEM16A is also reported to mediate the bradykinin‐induced nocifensive response and is required for generating nociceptive behaviours in mouse models of thermal pain (Jin et al., 2013; Liu et al., 2010). We therefore asked if the TMEM16A activator Eact could also evoke itch‐ and pain‐related behaviours in vivo. Indeed, i.d. injection of Eact into the rostral back of mice produced an intense scratching response (Figure 1A). Moreover, injection of Eact into hind paws of mice immediately elicited a nocifensive response, suggesting that Eact produces both acute pain and itch sensations (Figure 1B). Furthermore, paw injection of Eact evoked a robust and sustained thermal hypersensitivity lasting for at least 60 min (Figure 1C). Surprisingly, when Eact was co‐applied with a selective TMEM16A inhibitor T16Ainh‐A01 (A01) (Davis et al., 2013), the Eact‐elicited itch‐related and pain‐related behaviours were only partially reversed (Supporting Information Fig. S1), suggesting the existence of TMEM16A‐independent targets for Eact.

Figure 1
Genetic ablation or pharmacological blockade of TRPV1 function severely attenuates or abolishes Eact‐induced itch‐ and pain‐related behaviours. (A) I.d. injection of 50 μL Eact (4.67 mM) into the rostral ...

The Eact‐induced itch‐ and pain‐related behaviours are reminiscent of the TRPV1‐mediated histamine‐dependent itch, capsaicin‐induced nocifensive response and thermal hyperalgesia under inflammatory conditions. We thus speculated that TRPV1 might be involved in Eact‐induced sensory hypersensitivity. To address this possibility, we used a selective TRPV1 antagonist, AMG9810, which substantially inhibits capsaicin‐induced nocifensive response and Freund's complete adjuvant‐induced thermal hyperalgesia (Gavva et al., 2005; Ro et al., 2009). When AMG9810 (50 mg kg−1, i.p. injection) was applied 30 min before Eact administration, it severely attenuated Eact‐induced scratching, nocifensive response and thermal hypersensitivity (Figure 1). Even more surprisingly, genetic ablation of the TRPV1 function completely abolished Eact‐elicited pain‐related and itch‐related behaviours, suggesting that TRPV1 might be the prominent receptor for Eact‐induced sensory hypersensitivity in vivo (Figure 1).

Eact activates recombinant TRPV1 channels expressed in heterologous cells

There are a few possibilities for the involvement of TRPV1 in Eact‐elicited sensory hypersensitivity, for instance, TRPV1 could act as a mediator of the TMEM16A signalling complex as recent studies showed that TMEM16A functionally interacts with TRP channels including TRPV1 and TRPV4 (Takayama et al., 2014; Takayama et al., 2015), and we also found that co‐expression of TMEM16A with TRPV1 could enhance capsaicin‐activated currents in HEK293T cells (Supporting Information Fig. S2). The other possibility is that Eact could directly activate TRPV1. We tested this possibility in mTRPV1‐expressing HEK293T cells using live cell Ca2 + imaging and whole‐cell patch‐clamp recordings. Indeed, Eact (at 100 μM) evoked a substantial increase in [Ca2 +]i in cells expressing mTRPV1, which were also activated by the selective TRPV1 agonist capsaicin (500 nM) (Figure 2A). In marked contrast, Eact had no effect on HEK293 cells transiently transfected with other thermally activated TRP channels (ThermoTRPs) including human TRPA1, human TRPM8 and rat TRPV4, suggesting that Eact selectively activates TRPV1 among the ThermoTRPs tested.

Figure 2
Eact activates recombinant TRPV1. (A) The representative trace on top shows that Eact (100 μM) evoked an increase in [Ca2 +]i in a HEK293T cell transiently transfected with mTRPV1, which also responded to capsaicin (Cap: 500 nM). ...

Consistent with the results from the Ca2 + imaging studies, Eact also activated membrane currents in mTRPV1‐expressing HEK293T cells in a concentration‐dependent manner with an EC50 of 11.6 ± 2.5 μM (Figure 2B–D). The current traces in response to a voltage ramp (−100 to +100 mV) for Eact were similar to that activated by capsaicin or camphor (Figure 2B and C) (Caterina et al., 1997; Xu et al., 2005). Although HEK293T cells express endogenous TMEM16A (Sala‐Rabanal et al., 2015), knockdown of TMEM16A by small interference RNA (siRNA) did not affect Eact activation of mTRPV1 in HEK293T cells (Supporting Information Fig. S3), suggesting that activation of TRPV1 by Eact does not require endogenously expressed TMEM16A in the HEK293T cells. The maximal response evoked by 100 μM Eact was 69.4 ± 0.9% of the response evoked by 3 μM capsaicin (the current density of 100 μM Eact was 404.0 ± 24.4 pA pF‐1, compared with 581.8 ± 33.9 pA pF‐1 by 3 μM capsaicin) (Figure 2D), indicating Eact has a lower efficacy than capsaicin to activate TRPV1. Furthermore, Eact increased single‐channel activities in inside‐out patches excised from the TRPV1‐expressing HEK293T cells (Figure 2E), suggesting that Eact directly activates recombinant TRPV1 in the absence of intracellular signalling molecules.

Molecular basis of Eact activation of TRPV1

The TRPV1 channel is an allosterically regulated modular protein and activators like capsaicin, protons and noxious temperature stimuli activate TRPV1 through distinct protein domains (Aneiros et al., 2011). To investigate the amino acid residues involved in Eact activation of TRPV1, we made TRPV1 mutants in which activation by temperature (N629K, N653T/N654T), proton (E601Q and E649Q) or capsaicin (R115A, Y512A, S513Y, Y512A/S513Y, M548L and T551A) was severely impaired. We obtained concentration‐response curves of Eact‐activated whole‐cell currents and compared EC50 values of WT and mutant TRPV1 constructs individually transfected in HEK293T cells. We found that Eact‐activated currents were severely attenuated in the S513Y, Y512A/S513Y mutants in which the Eact EC50 values were increased by at least sixfold when compared with WT TRPV1 (Figure 3B and Table 1). Additionally, EC50 values for Eact in mutants S513A, M548L and T550A were also increased, although not as high as that in the S513Y, Y512A/S513Y mutants (Figure 3B and Table 1). In contrast, TRPV1 mutants with impaired proton or heat activation had comparable Eact EC50 values as the WT TRPV1 (Figure 3A and Table 1). These results suggest that the domains that participated in capsaicin activation of TRPV1 are also required for the interaction between Eact and TRPV1.

Figure 3
Structural requirements for Eact activation of TRPV1. (A) Concentration‐response curves of Eact‐activated outward currents at +60 mV in WT and TRPV1 mutants with disrupted domains for proton or heat activation of TRPV1. (B) Concentration‐response ...
Table 1
EC50 values and Hill coefficients of Eact‐activated response in WT and TRPV1 mutants

Eact activates endogenous TRPV1 expressed by DRG neurons

To further address whether Eact could activate native TRPV1 channels, we examined the effect of Eact on membrane currents and [Ca2 +]i responses in dissociated mouse DRG neurons. Administration of Eact (100 μM) activated a large outwardly rectifying current in cultured small‐diameter WT DRG neurons (Figure 4A and B). The Eact‐activated current was substantially reduced by co‐application of TRPV1 inhibitors AMG9810 (0.1 μM) and capsazepine (10 μM), and was absent from the Trpv1 / DRG neurons (Figure 4, Supporting Information Fig. S4). Moreover, co‐application of a selective TMEM16A inhibitor A01 did not significantly affect the Eact‐activated current in WT DRG neurons (Supporting Information Fig. S5). Furthermore, the Eact‐activated current persisted in WT DRG neurons recorded with a Clfree pipette solution (Supporting Information Fig. S6), suggesting that the Eact‐activated current is not carried by chloride. These results suggest that TRPV1 is a Eact receptor in cultured DRG neurons, and activation of TRPV1 by Eact does not require TMEM16A.

Figure 4
Pharmacological inhibition and genetic ablation of TRPV1 function inhibits Eact‐activated membrane currents in DRG neurons. (A) Representative current traces show that Eact (100 μM) activated an outward current at +60 mV ...

Additionally, consistent with the Ca2 + imaging results from the mTRPV1‐expressing HEK293T cells, bath application of Eact (100 μM) alone elicited a robust [Ca2 +]i response in 32.7 ± 2.2% of DRG neurons tested, all of which responded to capsaicin (Figure 5A and B). Interestingly, capsaicin (500 nM) increased [Ca2 +]i in a larger population of DRG neurons (51.8 ± 5.1%) than that activated by Eact, suggesting that not all TRPV1‐expressing neurons responded to Eact (Figure 5C). As expected, pharmacological inhibition by AMG9810 (0.1 μM) or genetic ablation of the TRPV1 function almost completely abolished the Eact‐evoked [Ca2 +]i responses, while allyl isothiocyanate still elicited comparable [Ca2 +]i responses in both wt. and TRPV1‐deficient DRG neurons (Figure 5B and C). Collectively, these results suggest that TRPV1 is the primary mediator of Eact‐induced acute excitatory responses in the primary sensory neurons.

Figure 5
Eact evokes [Ca2 +]i responses in a subset of DRG neurons in a TRPV1‐dependent manner. (A) Representative Fura‐2 ratiometric images of cultured DRG neurons show that Eact‐evoked [Ca2 +]i response in a subset of DRG neurons from ...

Discussion

Our study provided the first evidence that Eact, a selective activator of TMEM16A, directly activates the TRPV1 channels in sensory nociceptors and produces itch, acute nociception and thermal hypersensitivity. Eact interacts with the cytosolic amino acid residues required for capsaicin activation of TRPV1. These results suggest that Eact is a potent activator of the itch‐ and pain‐initiating TRPV1 channel, although this compound had been shown to be selective for TMEM16A.

Our data show that Eact induced a robust [Ca2 +]i response in HEK293T cells transiently transfected with TRPV1, but not TRPA1, TRPM8 or TRPV4. The effect of Eact on the mTRPV1‐expressing HEK293T cells was not affected by siRNA knockdown of the endogenous TMEM16A. Additionally, Eact activated single channel currents in the inside‐out membrane patches isolated from the mTRPV1‐expressing HEK293T cells, suggesting that this agonistic action of Eact is most likely resulting from a direct effect of the drug on TRPV1 but not through releasing intracellular second messengers. Moreover, Eact also activated membrane currents and evoked a [Ca2 +]i response in DRG neurons through TRPV1 as supported by both pharmacological inhibition and genetic ablation studies.

TRPV1 is a critical molecular sensor for noxious heat and a key contributor to inflammatory thermal hyperalgesia (Caterina et al., 2000; Davis et al., 2000; Tominaga et al., 1998). Although some studies have reported that mice lacking TRPV1 display heat avoidance behaviours, TRPV1‐deficient neurons still respond to heat stimulation (Basbaum et al., 2009; Woodbury et al., 2004), suggesting the presence of additional heat sensors. TMEM16A was recently identified as a heat‐sensitive channel that was activated by temperatures greater than 44°C (Cho et al., 2012). Moreover, nearly 78% of TMEM16A‐immunoreactive small‐diameter DRG neurons express TRPV1 (Cho et al., 2012). RNAi knockdown or genetic deletion of TMEM16A from DRG neurons also substantially attenuated thermal hyperalgesia in both inflammatory and neuropathic pain models (Lee et al., 2014; Liu et al., 2010). Moreover, a recent study also showed that Eact activated DRG neurons and induced pain response, both of which were suppressed by the TMEM16A inhibitor A01 (Deba and Bessac, 2015). Indeed, we also found that co‐expression of TMEM16A potentiated capsaicin‐activated currents in mTRPV1‐expressing HEK293T cells, suggesting that Eact activation of TMEM16A might also contribute to Eact‐elicited sensory hypersensitivity. On the other hand, although the selective TMEM16A inhibitor A01 was able to partially suppress Eact‐elicited pain‐ and itch‐related behaviours in vivo, it did not significantly suppress the Eact‐activated whole‐cell membrane current in DRG neurons. Furthermore, we could not record the Eact‐activated current in the Trpv1 / DRG neurons. One explanation for our findings is that TRPV1 function is required for TMEM16A‐mediated responses in the primary nociceptors. Indeed, TRPV1 and TMEM16A have been shown to interact physically and TRPV1‐mediated Ca2 + influx subsequently activates TMEM16A (Takayama et al., 2015). Moreover, TRPV4 is also found to functionally interact with TMEM16A to modulate water transport in the choroid plexus (Takayama et al., 2014). Therefore, our results do not argue against the existence of functional TMEM16A in the primary sensory neurons but support the hypothesis that Eact activation of TRPV1 is an upstream event of TMEM16A activation in the same DRG neurons. Thus, although A01 could not suppress Eact activation of TRPV1, it might be able to inhibit downstream events mediated by TMEM16A.

Although different experimental settings, cell culture conditions, and differences between HEK293T cells and DRG neurons might partly account for the discrepancy of Eact responses in DRG and heterologous cells between these two studies (Deba and Bessac, 2015), our results using TRPV1 knockout mice clearly demonstrated that Eact‐induced excitation and sensory hypersensitivity require functional TRPV1 channels, suggesting that Eact is not an ideal tool to dissect the function of TMEM16A in the primary sensory neurons because it could directly activate TRPV1 in the same cells. Because TRPV1 is a dominant initiator of pain and itch in the primary sensory neurons, Eact activation of TRPV1 could override TMEM16A‐mediated Eact responses. Future studies using TMEM16A knockout mice will be required to provide a clear conclusion.

TRPV1 is also a critical itch receptor downstream of histamine H1 receptor signalling (Imamachi et al., 2009). Previous studies also showed that itch‐causing substances, such as ET‐1 and histamine evoked inward chloride currents in the mTMEM16A‐expressing HEK293 cells (Cho et al., 2012; Yang et al., 2008). Furthermore, the blockers of sodium–potassium–chloride co‐transporter‐1, which accumulates Cl inside of cells, reduced histamine‐induced itch and flare in humans (Willis et al., 2004), suggesting that TMEM16A may be critically involved in itch initiation in sensory neurons. However, although we found that i.d. injection of Eact into the rostral back could induce itch‐related behaviours, these scratching responses were almost abolished by pharmacological inhibition or genetic ablation of the TRPV1 function. Although A01 also partially suppressed the Eact‐elicited scratching, as discussed above, the exact role of TMEM16A in itch signalling transmission warrants further studies using the TMEM16A knockouts.

Taken together, our data indicate that the TMEM16A activator Eact activates both native and recombinant TRPV1 channels. This new finding should lead to a more careful interpretation of the effects of Eact on the excitability of neurons that are known to express TRPV1, for instance, primary nociceptors from the DRG or TG. Furthermore, the agonistic action of Eact on TRPV1 should also be taken into consideration for interpretation of the results related to TMEM16A‐mediated pain‐ and itch‐related behaviours if Eact is used as a pharmacological tool. Moreover, the sensory hypersensitivity elicited by Eact and potentially other structurally‐related Ca2 +‐activated chloride channel activators might present an unwanted side effect for their applications to treat cystic fibrosis and related diseases.

Author contributions

S.L., J.F., J.L. and P.Y. performed the research; S.L. and H.H. designed the research; S.L. and J.F. analysed the data; S.L., T.J.B. and H.H. wrote the paper.

Conflict of interest

The authors declare no conflicts of interest.

Declaration of transparency and scientific rigour

This Declaration acknowledges that this paper adheres to the principles for transparent reporting and scientific rigour of preclinical research recommended by funding agencies, publishers and other organisations engaged with supporting research.

Supporting information

Figure S1 Pharmacological blockade of TMEM16A function attenuated Eact‐induced itch‐ and pain‐related behaviors. (A) Co‐application of T16Ainh‐A01 (A01, 10 μg) suppressed the scratching response elicited by intradermal injection of 50 μl Eact (4.67 mM) into the rostral back. Please note that 10 μg is the highest dose for A01 used in vivo (Pineda‐Farias et al., 2015). (B) Co‐application of T16Ainh‐A01 (A01, 10 μg) suppressed the flinching and licking behaviors produced by intraplantar injection of 20 μl of Eact (4.67 mM). (C) Time course of thermal hypersensitivity in animals treated with Eact (intraplantar, 20 μl, 4.67 mM). Co‐applied A01 (0.48 M) attenuated Eact‐induced decrease of paw withdrawal latency. *p<0.05, **p<0.01, ***p<0.001 Eact group versus vehicle group; +p<0.05, ++p<0.01 Eact group versus Eact + A01 group; #p<0.05, ##p<0.01 Eact + A01 group versus A01 group; n = 7‐8 mice per group. A01 = T16Ainh‐A01.

Figure S2 TMEM16A increases capsaicin response when co‐expressed with mTRPV1 in HEK293T cells. (A) and (B) Representative traces show that capsaicin (0.3 μM) activated an outward current at +60 mV and an inward current at ‐60 mV in HEK293T cells expressing mTRPV1 alone (A) or co‐expressing mTRPV1 and m TMEM16A (B). (C) Comparison of the peak capsaicin current densities between cells expressing mTRPV1 alone and cells co‐expressing mTRPV1 and m TMEM16A (*p<0.05, n = 7‐8 cells per group).

Figure S3 siRNA knockdown of TMEM16A did not affect Eact‐activated membrane currents in mTRPV1‐expressing HEK293T cells. (A) Immunoblotting results show that transfection with small interfering RNA (siRNA, 200 nM) against TMEM16A, but not scrambled siRNA control (Scr. siRNA, 200 nM), markedly reduced TMEM16A protein expression in the mTRPV1‐expressing HEK293T cells. ***p<0.001; n=3 per group. (B) Representative Eact (10 μM)‐activated current traces in response to voltage ramp from ‐100 mV to +100 mV in mTRPV1‐expressing HEK293T cell transfected with either scrambled siRNA control or siRNA against TMEM16A. (C) Quantification of Eact (10 μM)‐activated current responses in mTRPV1‐expressing HEK293T transfected with scrambled siRNA control or siRNA against TMEM16A. Cells were voltage‐clamped at ‐60 mV (NS: no significant difference, n = 6 per group).

Figure S4 The TRPV1 inhibitor capsazepine nearly abolished Eact‐activated membrane currents in cultured DRG neurons. (A) Representative current traces show that Eact (100 μM) activated an outward current at +60 mV and an inward current at ‐60 mV in a wt DRG neuron. The whole‐cell currents evoked by Eact were substantially suppressed by capsazepine (10 μM). The inhibitory effect of capsazepine was partially reversible after washout. (B) Current‐voltage relationship of Eact‐activated currents taken at the time points specified in A. (C) Quantification of averaged Eact‐activated currents with and without capsazepine in DRG neurons voltage‐clamped at ‐60 mV. ***P < 0.001, NS: no significant difference, n = 10.

Figure S5 A01, the selective inhibitor of TMEM16A, did not inhibit Eact‐activated membrane currents in DRG neurons. (A) Representative current traces show that Eact (100 μM) activated an outward current at +60 mV and an inward current at ‐60 mV in a wild type DRG neuron. The whole‐cell currents evoked by Eact were slightly suppressed by A01 (10 μM). (B) Current‐voltage relationship of Eact‐activated current taken at the time points specified in A. (C) Quantification of Eact current densities in DRG neurons voltage‐clamped at ‐60 mV. There was no significant difference among Eact responses (n = 9). NS: no significant difference, A01 = T16Ainh‐A01.

Figure S6 Eact‐activated membrane currents in cultured sensory neurons are not Cl currents. (A) Representative current traces show that Eact (100 μM) activated an outward current at +60 mV and an inward current at ‐60 mV in a wt DRG neuron recorded with a Cl‐free pipette solution. (B) Current‐voltage relationship of Eact‐activated currents taken at the time points specified in A. (C) Comparison of the peak Eact‐activated current densities in DRG neurons with 140 mM Cl (High [Cl]i) pipette solution and 0 mM Cl (Free [Cl]i) pipette solution voltage‐clamped at ‐60 mV (NS: no significant difference, n = 9‐10 per group).

Supporting info item

Acknowledgements

This work was supported partly by grants from the National Institutes of Health, R01RGM101218 and R01DK103901 (to H. H.), and R01HL119813 (to T. J. B.), The Center for the Study of Itch of Department of Anesthesiology at Washington University School of Medicine to H. H. and China Scholarship funding to S. L.

Notes

Liu S., Feng J., Luo J., Yang P., Brett T. J., and Hu H. (2016) Eact, a small molecule activator of TMEM16A, activates TRPV1 and elicits pain‐ and itch‐related behaviours. British Journal of Pharmacology, 173: 1208–1218. doi: 10.1111/bph.13420.

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