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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Ann N Y Acad Sci. Author manuscript; available in PMC 2017 April 1.
Published in final edited form as:
PMCID: PMC4938003
NIHMSID: NIHMS753607

Niche heterogeneity in the bone marrow

Abstract

In adult mammals, hematopoietic stem cells (HSCs) are defined by their abilities to self-renew and to differentiate to form all blood cell lineages. These rare multipotent cells occupy specific locations in the bone marrow microenvironment. The specific microenvironment regulating HSCs, commonly referred to as the niche, comprises multiple cell types whose exact contributions are under active investigation. Understanding cellular cross talk involving HSCs in the bone marrow microenvironment is of fundamental importance for harnessing therapies against benign and malignant blood diseases. In this review, we summarize and evaluate recent advances in our understanding of niche heterogeneity and its influence on HSC function.

Keywords: BM, microenvironment, niche, stem cells

Introduction

Hematopoietic stem cells (HSCs) represent a rare subpopulation of hematopoietic cells, mostly residing in the bone marrow (BM),1,2 that reign at the top of the hematopoietic hierarchy. These cells are endowed with regulated quiescence3,4 and a capacity for long-lived self-renewal that preserves the multipotency of mother cells and sustains the generation of all blood cell types throughout life.510 Their capacity to rebuild the entire adult hematopoietic system makes HSCs invaluable for the treatment of multiple hematopoietic disorders. For several decades, the therapeutic potential of HSCs has been applied to regenerate the hematopoietic system via BM transplantation procedures.11 Understanding the signaling mechanisms that determine HSC fate will be crucial for the success of clinical applications. Recent investigations using genetically modified mouse models suggest that decisions of HSC self-renewal and multilineage differentiation are dependent on the interaction with the surrounding microenvironment, also termed niche.1214 Thus, proquiescence, prodifferentiation or pro-self-renewal microenvironments define HSC fate.15,16 Experimental evidence has shown that deregulation of those microenvironmental regulatory mechanisms plays a key pathogenic role in a variety of hematopoietic diseases.5 In recent years, several cell types have been identified as potential niche-supporting cells for HSCs, regulating HSC activity by supplying various cytokines and retention factors. Here, we present an overview of the current knowledge on the variety of BM cell types and their effects on HSCs.

Heterogeneity of HSCs and niches

HSCs represent a functionally heterogeneous cell population in their degree of self-renewal,17,18 life span,1921 and differentiation capabilities.22 Self-renewal heterogeneity is manifested by distinct capacities of long-term (LT-HSC), intermediate-term (IT-HSC), and short-term repopulating HSCs (ST-HSC),23 which have been distinguished by differential abilities to engraft in vivo into irradiated hosts and to maintain multilineage hematopoiesis for extended time periods and/or by serial transplantation.24 A separate challenge for isolating HSC subpopulations is that they may represent a continuum of states of the same cell, which may be challenging to isolate, although a few markers have been shown to define distinct properties, such as CD150 for LT-HSCs,25,26 integrin α2 (CD49b) for IT-HSCs,27 or platelet integrin CD41 (also known as Itga2b) for myeloid-biased adult HSCs.28

Single purified HSCs exhibit large fluctuations in their contributions to myeloid and lymphoid lineages.19 Subsequent studies demonstrated distinct biases of HSCs, with consistent preponderance to generate lymphoid or myeloid cells.2931 Interestingly, on the basis of Hoechst dye–efflux capability, myeloid- and lymphoid-biased HSC subsets differ in their responsiveness to TGF-ß1. This cytokine induces proliferation of myeloid-biased HSCs while inhibiting proliferation of lymphoid-biased HSCs.32 More recently, a platelet-biased HSC subset was identified through the use of a von Willebrand factor (vWF)–EGFP mouse system.33 High expression of vWF, a blood glycoprotein mediating platelet aggregation, was reported in HSC-enriched BM cells.26 Transplantation of vWF–EGFP+ and vWF–EGFP cells from the CD150+CD48CD34 KSL fraction of adult BM achieved long-term hematopoietic reconstitution in recipient mice. This study also suggested that vWF+ HSCs are primed toward the megakaryocytic lineage.33

Very little is known about the extrinsic regulation of HSC subpopulations. For instance, it is unclear whether the niches for lymphoid-, myeloid-, or megakaryocyte-biased HSCs differ. The functional heterogeneity of HSCs points to the potential for matching heterogeneity in the microenvironmental influences that support the function and behavior of these HSC subsets. The reader is referred to excellent reviews that discuss these HSC subsets in detail.3436 The remainder of the review will focus on niche heterogeneity.

The anatomy of the BM may shed light on the specific microenvironments where HSCs may reside and are regulated. BM is found within the central cavities of long and axial bones. The trabecular regions of the metaphysis have been shown to be the preferred site of HSC homing compared to the epiphysis or diaphysis.37 The inner surface of the bone cavities is covered by an endosteal lining consisting of osteoblasts, osteoclasts, and a single layer of flat bone-lining cells supported by a thin layer of reticular connective tissue. The endosteal region may present a greater concentration of free calcium ions from continuous bone remodeling.38,39 Several studies have noted that HSCs tend to localize peripherally near the bone surface rather than in the central medullary regions.38,40,41 Moreover, it has been suggested that HSCs at the endosteal location have greater self-renewal capacity than those in the central marrow cavity,42 suggesting the presence of a distinct microenvironment in this region. However, other recent analyses have suggested that HSCs may be randomly distributed in the BM.43 Interestingly, aged HSCs localize to sites further away from the endosteum compared with young HSCs,44 suggesting that HSC location is affected by aging.

The BM is served by numerous blood vessels of various sizes entering it through the cortical bone via nutrient canals.45 Lymphatic drainage is absent in the BM.46 The blood supplies of the bone and BM are interconnected through an endosteal network of vessels. Arteries give rise to a multitude of small, thin-walled arterioles that extend outwardly toward the cortical bone and sinusoids that pervade the central and endosteal marrow. Arterioles are small resistance vessels that, unlike other vessels in the BM, are wrapped circumferentially by one or more layers of smooth muscle cells.47,48 They are preferentially located close to the endosteal region of the BM.41 Nerve bundles follow the arterioles, with branches serving smooth muscle cells or terminating in the hematopoietic tissue among hematopoietic cells.49 The sinusoids form a permeable barrier for the passage of mature blood cells into the circulation. In contrast to arterioles, sinusoids are evenly distributed through the whole BM cavity and are not innervated. Both BM arteriolar and sinusoidal endothelial cells are surrounded by perivascular cells.50

Besides blood vessels and nerves, the BM tissue consists of a variety of cellular subtypes among hematopoietic and non-hematopoietic cells. Hematopoietic cells are most likely not randomly arranged but demonstrate a specific organization within the tissue.51 For instance, erythropoiesis takes place in distinct anatomical units (erythroblastic islands),5254 and a subset of HSCs is located near megakaryocytes.55,56

A niche supporting HSCs identified in close proximity to blood vessels in the adult BM has been called the perivascular niche.25,41 The perivascular niche itself is heterogeneous and contains distinct cell types. A recent study showed that dormant (quiescent) HSCs reside specifically in the proximity of arterioles rather than sinusoids, proposing that there are separate, spatially distinct perivascular niches for quiescent and proliferating HSCs in the BM.41

During development, perivascular hematopoietic niches have been also described to be present in other organs, like the placenta57 and spleen.25 Additionally, a recent report showed that HSCs expand around fetal liver portal vessels, suggesting that blood vessels provide an adaptive niche, serving hematopoiesis at multiple developmental stages of mammalian life.58

Heterogeneity of cells of the niche

Non-hematopoietic types

Osteoblasts

Cells of the osteoblastic lineage have been proposed to function as critical modulators of HSCs in the BM.59 In vitro culture experiments have suggested that osteoblastic cell lines can expand the number of HSCs two- to fourfold.60 In addition, when co-transplanted with HSCs, osteoblasts can increase the engraftment rate.61 Osteoblasts produce a wide array of growth factors and cytokines, important for HSCs maturation.60,62 Osteopontin, an osteoblast-secreted protein, participates in HSC location and is a negative regulator of their proliferation.63,64 Furthermore, angiopoietin-1 expressed by osteoblasts has been suggested to regulate HSC numbers through the activation of Tie-2 signaling pathway,65 while Jagged 1, a Notch receptor ligand, supports an increase in HSC numbers.66 Mutant mice with conditional deletion of BMP receptor type IA or genetic alteration to produce osteolineage-specific activated PTH led to abnormal bone formation with increased osteoblast numbers that correlated with increased HSC numbers in the BM.66 Genetic depletion of osteolineage cells by the use of GCV in transgenic mice expressing herpesvirus thymidine kinase gene under the control of a constitutive 2.3-kb fragment of the rat α(1) type I collagen promoter leads to BM hypocellularity and extramedullary hematopoiesis within the spleen and liver.67 After GCV is withdrawn, osteoblasts reappear in the bone compartment, together with a decrease in extramedullary and a recovery of medullary hematopoiesis.67

Since constitutive promoter expression may not be specific to mature osteoblasts but could be expressed up to the MSC stage,68 specificities with careful lineage tracing to assess transgenic expression and recombination are needed to define osteolineage cells. Indeed, adipo-osteogenic progenitors69 and Nestin+ cells70 express the osteogenic proteins Runx2 and Osterix. In keeping with this idea, osteoblastic expansion does not always promote HSC expansion in the BM.71 Strontium, which inhibits both osteoclast resorbing activity and osteoblast bone-forming activity,72 did not increase the number or frequency of HSCs when administered in doses higher than normally detected in the body.71 Osteocytes, derived from osteoblasts that became embedded within the bone matrix, appear to have an inhibitory effect on HSC support.73 More recent studies have shown that cells of the osteoblastic lineage may support lymphopoiesis. Elimination of osteoblasts in Col2.3-TK transgenic mice depleted pre-pro-B and pro-B cells from the BM74 without affecting the HSCs.75 Depleting CXCL12 from osteoblasts in Col2.3-cre mice leads to loss of lymphoid progenitors in the BM without affecting the number of HSCs.76 Thus, the exact function of osteoblasts in hematopoiesis remains unclear; most recent studies indicate that they are dispensable for HSC maintenance and more important to lymphoid progenitors.

Endothelial cells

The endothelium forms the inner cellular lining of blood vessels.77 Because most HSCs were found to be associated with blood vessels, endothelial cells were suggested to play important roles in HSC maintenance in sinusoids.78 An intact vasculature is necessary for HSC recovery and hematopoietic reconstitution following total body irradiation and BM transplantation.7981 Several studies described the expansion of HSCs on an endothelial cell feeder system in vitro.8286 In addition, BM endothelial cells have been proposed to produce soluble factors that have the ability to promote HSCs self-renewal and regeneration in vivo.80,81,8790

HSCs are found in close contact with endothelial cells at all developmental stages. Endothelial cells harvested from embryonic tissues have been shown to support the expansion of adult HSC cells in vitro.91,92 On the contrary, endothelial cells isolated from adult non-hematopoietic organs do not display an HSC-supportive activity in vitro.93 These observations indicate tissue-specific functional characteristics of endothelial cells.

Deletion of glycoprotein 130 from endothelial and hematopoietic cells with Tie2-cre mice resulted in BM dysfunction and severe anemia by adulthood. Hematopoietic defects remained after transplantation of wild-type BM into irradiated glycoprotein 130–deficient mice, while normal hematopoiesis was reconstituted after transplantation of glycoprotein 130–deficient BM into irradiated wild-type mice, indicating that glycoprotein 130 expression on endothelial cells rather than hematopoietic cells influenced hematopoiesis.94 Deletion of Scf in the same Tie2-cre mice led to reduced HSC frequencies with diminished repopulation capacities after BM transplantation, suggesting that SCF derived from endothelial cells contributes to HSC maintenance.95 Additionally, despite the fact that depletion of CXCL12 from endothelial cells using the same mouse model had no effect on the number of HSCs, it resulted in a slight decrease of long-term repopulating activity.96

Endothelial cells are heterogeneous in their morphology, gene expression, antigen composition, distribution, and function. Endothelial cell phenotypes vary between different tissues, as well as between different segments of the vasculature within the same tissue.97,98 It remains unknown, for instance, whether arteriolar and sinusoidal endothelial cells differ. Elucidating their molecular differences in the BM may reveal novel concepts about these cells’ role in the HSC niche.98

Pericytes

Pericytes have long projections that encircle the blood vessel wall in almost all tissues.99106 They are defined by their anatomical locations in combination with several molecular markers, such as platelet-derived growth factor receptor β (PDGFRβ), CD146, neuron-glial 2 (NG2), αSMA, desmin, and Nestin. However, marker expression is highly dependent on the tissue type, and could often be affected by the pathologic state of the organ.99

Pericytes have been suggested to influence HSCs in both mice and humans.70,95,107 Using Nestin–GFP transgenic mice, Nestin+ perivascular cells have been defined as central components of the HSC niche, regulating HSCs via the expression of C-X-C motif chemokine 12 (CXCL12) and stem cell factor (SCF)70, 108. In vivo ablation of those cells in the BM significantly reduces the number of HSCs.70 Cells located in a pericytic position close to the vasculature enriched in CXCL12 have been described and named CXCL-12 abundant reticular (CAR) cells.50 The transcription factor forkhead box C1 (FoxC1) in CAR cells has recently been identified as essential for maintenance of HSCs in vivo.109 Selective ablation of CAR cells in the BM leads to reduction of HSCs.69 Additionally, PDGFRα+Sca-1+ (PαS) cells,110 located in the arterial perivascular space,111 and leptin receptor–expressing pericytes have been suggested to regulate HSC maintenance in the BM.95,96 These cells exhibit common characteristics in that leptin receptor+ stromal cells overlap with CAR cells76 and represent a large subset (nearly 90%) of Nestin–GFP+ cells in the BM. Nestin–GFP+ cells also overlap with PαS cells, although most PαS cells are associated with the bone itself (most marrow Nestin–GFP+ cells do not express Sca-1).108

There are at least two varieties of BM pericytes according to their location in the blood vessels: arteriolar and sinusoidal.41 Sinusoids are structurally different from arterioles in that they are lined by a single layer of endothelium, while arterioles are thicker-walled blood vessels.112 Arteriolar and sinusoidal pericytes can be separated in Nestin–GFP transgenic mice according to Nestin-GFP transgene expression level.41 Sinusoidal pericytes are more numerous and reticular in shape. They express lower levels of the Nestin–GFP transgene, whereas rare arteriolar Nestin–GFPbright pericytes exhibit a classic pericyte morphology. Additionally, arteriolar pericytes express the pericytic marker NG2 proteoglycan, but do not express leptin receptor; while sinusoidal pericytes express leptin receptor, but lack NG2 expression.41 The embryonic origin and the developmental relationships of BM pericyte subpopulations have yet to be elucidated. Interestingly, an evaluation of the cell cycle status demonstrated that arteriolar pericytes are largely quiescent.41 Tridimensional imaging of the adult mouse BM has revealed that the majority of dormant HSCs are situated close to arterioles; and genetic depletion of arteriolar pericytes resulted in migration of HSCs away from the arterioles, switching them into non-quiescent status.41 This suggests that arteriolar pericytes promote HSC dormancy, essential for HSC maintenance in the BM.41 Nevertheless, the molecular mechanisms by which arteriolar pericytes regulate HSC quiescence remain to be clearly defined.

Adipocytes

It has been suggested that BM fat is unlike white and brown fat of other tissues.113115 Adipocytes appear dispersed within the BM, instead of being grouped into lobules, and are smaller than visceral and subcutaneous adipocytes.116,117 Although older studies suggested that adipocytes were passive occupants of the BM,118 simply filling the spaces after trabecular bone loss, a regulatory role in the BM has emerged with recent studies.113,118,119 A specific marker for BM adipocytes has not yet been determined, but there are several differentially expressed genes in BM adipocytes compared to subcutaneous and epididymal adipocytes.120,121 A reduction of adipocyte numbers with increased size has been noted with disease and aging in the BM.113,119,122125 This also has been associated with a decrease in cellularity126 and induction of myeloid-biased differentiation in HSCs.1 Nevertheless, no direct link between an increase in adipose tissue and HSC myeloid bias during aging has been established.

Similar to adipocytes from other tissues, BM adipocytes are able to secrete cytokines, fatty acids, and hormones, and have a potential to influence the function of other neighboring cells in the BM microenvironment via paracrine mechanisms.119,124,127130 The details of those mechanisms are still being unraveled. For instance, in vitro, BM adipocytes secrete more leptin than subcutaneous adipocytes.131 Some investigators have suggested that this is attributable to an important role of leptin in myelopoiesis,132 where, in the diet-induced obesity mouse model, hematopoiesis is enhanced due the high leptin levels in the BM.130 However, in vitro studies differ on their conclusions about the effect of adipocytes on HSCs. While some studies show that adipocyte negative regulation can balance the positive effects of other cellular components of the BM niche on HSCs,133 others suggest that adipocytes can support HSC growth and differentiation in vitro.134,135 However, alterations of adipocyte number and volume in vivo have been reported to have no effect on HSCs.134,135

A great number of studies indicate that adipocytes inhibit HSCs function as well as hematopoietic reconstitution.136138 Lipid-filled BM adipocytes have been connected to the repression of growth and differentiation of HSCs136,139 and considered negative regulators of the hematopoietic niche.69,140 Hematopoietic recovery is improved following chemotherapy in mice with chemically inhibited adipogenesis.141 In mouse tail vertebrae, where BM cavities are densely filled with adipocytes, the few HSCs detected are largely quiescent.136 The adipocyte suppressive function has been attributed to the reduced production of granulocyte colony-stimulating factors (GM-CSF and G-CSF) and to the increased secretion of lipocalin-2 and neuropilin.136,142,143 Interestingly, at the same time that adipocytes prevent HSC expansion, they seem to positively affect HSCs via secretion of adiponectin and TNFα,144,145 playing a role in preserving the hematopoietic stem cell pool.136 Whether the role of BM adipocytes in the HSC niche differs under various physiological conditions, including aging or obesity, remains unclear.

Schwann cells

Schwann cells are the principal glial cells of the peripheral nervous system. In the BM, they are present in their unmyelinated form associated with sympathetic and sensory nerve fibers.70,146,147 Until recently, unmyelinated Schwann cells received relatively little scientific attention, with only a poor understanding having developed of their function outside the nervous system. Schwann cells maintain the ability to revert to an immature phenotype in response to injury and disease and, by doing so, they can then re-enter the cell cycle, proliferate, and affect the microenvironment in which they are located.148,149 Schwann cells were shown to produce several cytokines,150 and to express cytokine receptors as well.151

Recent studies have suggested that BM Schwann cells regulate the hibernation and activation of HSCs.152 In that study, using immunohistochemistry, the expression of active TGFβ was proposed to be restricted to BM Schwann cells.152 BM denervation reduced the number of cells producing active TGFβ, leading to a loss of HSCs from the BM.152 However, denervation not only affects Schwann cells; for instance, β-adrenergic signals from the sympathetic nervous system have been shown to regulate enforced49 and circadian HSC egress.153 Moreover, in addition to Schwann cells, other cells, including megakaryocytes, produce TGFβ in the BM.154 TGFβ1 derived from megakaryocytes has been shown to maintain HSC quiescence.56 Most of BM innervation—and Schwann cell ensheathing of those nerve fibers—runs along arterioles in the BM.155 As arterioles contain multiple cell types, the identity of the cell promoting HSC quiescence remains unknown. Interestingly, both pericytes and Schwann cells express NG2 proteoglycan and may contribute to the arteriolar HSC niche.41,156 It will be interesting to ascertain whether BM Schwann cells differ from Schwann cells from other tissues. Successful isolation of BM Schwann cells may enable the discovery of novel niche factors possibly expressed by those cells.

Nerves

Signals from the sympathetic nervous system have been identified as regulatory components of the HSC niche.13,157,158 Sympathetic nerves produce catecholamines, which are delivered to the BM microenvironment by the blood circulation or by secretion from the nerve endings acting in paracrine signaling.159 Sympathectomy achieved by treating mice with the neurotoxin 6-hydroxydopamine does not affect HSCs number,49,153,160,161 but it impairs mobilization in response to G-CSF.49 Under steady-state conditions, HSCs egress from the BM to enter the blood circulation predominantly in the morning in rodents (night in humans),153 and migrate to the tissues during the night in rodents (day in humans).162165 Adrenergic signaling reduces CXCL12 expression in the BM.153,166 Moreover, recent evidence shows that chemotherapy-induced injury of sympathetic nerves in the BM prevents hematopoietic recovery, suggesting that treatment with neuroprotective drugs during chemotherapy would preserve HSC function in the BM niche.161 Induction of sympathetic neuropathy by malignant cells has recently been demonstrated.167,168

HSCs express catecholaminergic receptors, suggesting that they are able to directly respond to signals from the sympathetic nervous system.169 Treatment of HSCs with dopamine agonists enhances colony formation in vitro, albeit only in the presence of G-CSF.169 Pretreatment of HSCs with dopamine agonists enhances their ability to engraft.169 Additionally, norepinephrine treatment of HSCs also enhances both colony-formation capabilities in vitro and engraftment in vivo.159,169 Additionally, the sympathetic nervous system adjacent to the dorsal aorta plays an important role in HSC specification during development.170 Overall, these studies suggest a contribution of the sympathetic nervous system in regulating the HSC niche.

Hematopoietic types

Macrophages

Macrophages play diverse roles in the bone and marrow. At the sites of bone remodeling, they are anatomically juxtaposed with endosteal osteoblasts and participate in bone mineralization.171173 Radio-resistant macrophages protect the HSC pool from exhaustion by producing prostaglandin E2 after irradiation.174 BM resident macrophages are defined based on differential expression of several molecular markers, such as Gr-1, F4/80, CD115, and CD169.175 Their numbers are reduced during G-CSF–induced HSC mobilization, and, following their loss, HSCs egress to the peripheral circulation.174,176

Macrophages promote HSC retention in the BM by regulating the expression of CXCL12 by Nestin–GFP+ MSCs via a soluble factor secreted by CD169+ macrophages.54 Recent studies have suggested that this factor was oncostatin M.177 Thus, macrophages and sympathetic nerves exert the opposite action to the niche, forming a regulatory loop.54 HSC retention in the BM and the spleen relies at least partially on a ligand for VCAM-1, integrin VLA-4,178180 which is expressed by macrophages. A recent study described that macrophages are important players in splenic HSC retention, as depleting macrophages using inducible diphtheria toxin receptor expression at the CD169 locus mice or silencing VCAM-1 in macrophages caused release of HSCs from the spleen.181 Additionally, a separate subpopulation of macrophages expressing high levels of α-smooth muscle actin and cyclooxygenase 2 was recently identified. This rare macrophage population synthesizes prostaglandin E2, which increases CXCL12 expression in Nestin-GFP+ MSCs182 and CXCR4 expression on HSCs,183 thus improving the survival and maintenance of HSCs in the BM. Moreover, macrophages have been suggested to regulate HSC egress from the BM after phagocytosis of aged neutrophils.184

Interestingly, macrophages are also key mediators of the neuroprotective effect of neuropeptide Y, and thus contribute to HSC survival in the BM. Neuropeptide Y regulates homeostasis in several tissues through Y receptors.185187 A recent study shows that neuropeptide Y deficiency impairs HSC survival and BM regeneration. Furthermore, pharmacological elevation of neuropeptide Y prevented the deficits, while neuropeptide Y injection into mice lacking the Y1 receptor specifically in macrophages did not rescue BM dysfunction.188

Macrophages are also involved in both steady-state and stress-induced erythropoiesis. In vivo macrophage depletion leads to a decreased number of erythroblasts in the BM and induces anemia with characteristics of iron-deficiency anemia, which is not corrected by iron supplementation. Macrophage depletion also delays erythropoietic recovery following acute blood loss, myeloablation, or challenge with hemoglobin-oxidizing phenylhydrazine (PHZ).53,54 Interestingly, polycythemia vera, which is characterized by elevated erythropoiesis, can be improved via macrophage ablation. Macrophage depletion reduces hematocrit and red blood cell counts in a mouse model of polycythemia vera driven by the JAK2V617F mutation.54 These studies suggest that macrophages are critical components of erythroid maturation in the steady state, as well as during erythropoietic rescue after stress and disease. It remains unknown how erythroblastic island macrophages differ functionally from other BM resident macrophages. Erythroblastic island macrophages thought to be very large (with diameter exceeding 15 μm) express F4/80 and do not express Mac1;189 however, a unique molecular marker for erythroblastic island macrophages remains to be discovered. Overall, these findings suggest that macrophages are key components of the BM niche promoting the maintenance and retention of HSCs.

Osteoclasts

Osteoclasts originate from hematopoietic progenitors via mononuclear phagocytes.190 They are the only cell type capable of bone resorption in the human body, allowing the renewal of the skeleton but also opening space in the BM for hematopoietic cells. Osteoclasts have been suggested to contribute to HSC release via enzyme secretion, enhancing mobilization.191 On the other hand, it has been hypothesized that osteoclastic bone resorption releases calcium, increasing its concentration at the endosteal region, which attracts and retains HSCs that express calcium-sensing receptors in the BM.39 Bone resorption also produces active TGFβ, which can act on HSCs.192 Osteoclast inhibition by bisphosphonates causes a reduction in the number of HSCs and delays hematopoietic recovery.193 Using a mouse model with loss of osteoclast activity, osteoclasts have been shown to regulate mesenchymal cell differentiation and HSC maintenance.194

Megakaryocytes

A subset of HSCs is located in close proximity to megakaryocytes in the BM.55,195,196 Several mouse models with increased number of megakaryocytes have also demonstrated increased bone mass.197 Thus, some studies have suggested a complex interaction between megakaryocytes, HSCs, and the osteolineage within the BM. Accordingly, after BM radioablation, host megakaryocytes are recruited to the endosteum, where osteoblasts undergo rapid expansion in response to the secretion of megakaryocyte-derived mesenchymal growth factors, such as platelet-derived growth factor-β (PDGF-β), to promote HSC engraftment and hematopoietic reconstitution after BM transplantation.198 The migration of megakaryocytes to the endosteum is thought to depend on thrombopoietin signaling, since the inhibition of c-Mpl reduces megakaryocyte migration after radioablative conditioning.198

More recent findings have identified a direct HSC regulation by megakaryocytes in steady-state hematopoiesis.55,56,195 Ablation of megakaryocytes reduces HSC engraftment and proliferation.195,198 Thrombopoietin administration to megakaryocyte-depleted mice restores the number of quiescent HSCs,195 suggesting that thrombopoietin may contribute to regulation of HSCs by megakaryocytes. Megakaryocytes produce high levels of TGFβ, which regulate HSCs.199 Conditional deletion of Tgfb1 in megakaryocytes increases HSC activation and proliferation in young mice.56 In addition, TGFβ injection into megakaryocyte-depleted mice restores HSC quiescence.56 Under homeostatic conditions, megakaryocytes maintain HSC quiescence through TGFβ signaling; while under stress megakaryocytes promote HSC expansion via FGF-1 production.56 CXCL4, which is produced by megakaryocytes, negatively regulates HSC proliferation, reduces HSC numbers, and decreases engraftment.55 An increase in HSC number, proliferation, and repopulating activity was observed in CXCL4 knockout mice.55 As megakaryocytes produce multiple cytokines (e.g., thrombopoietin, TGFβ, and CXCL4), it is possible that their effect on HSCs results from the balance of all those and probably more molecules. Interestingly, megakaryocytes physically associate with approximately 20% of HSCs in the BM.55 It remains to be studied whether the function of those HSCs differs from that of the rest of the HSCs in the BM. Overall, these observations confirm that megakaryocytes serve as HSC-derived niche cells directly regulating HSC function.

Lymphocytes

Lymphocytes, essential for both cell-mediated and antibody-mediated immunity, are widely distributed throughout the BM parenchyma and make up a major fraction of total BM mononuclear cells.200 Lymphocytes have been suggested to influence hematopoiesis, potentially through direct cellular interactions with the HSCs.201 Natural killer cells have been suggested to play a negative role in HSC differentiation.202 Experiments in adult mice harboring a null mutation in the common γ chain indicated that cytokines secreted by activated T cells in the BM modulate normal hematopoiesis.203 Even though, in the absence of activated BM, CD4+ T cell HSCs can give rise to committed myeloid progenitors, these progenitors exhibit impaired ability to complete their differentiation program and give rise to mature cells.204 After adoptive transfer of CD4+ T cells, this defective myeloid differentiation is restored in T cell–deficient mice, suggesting that CD4+ T cells are essential to the maintenance of basal hematopoiesis in the BM.204

Regulatory T cells make up one third of all CD4+ T cells in the BM.205,206 Depletion experiments and co-transfer of BM with regulatory T cells indicated that these cells suppress colony formation and myeloid differentiation of HSCs.207 Moreover, FoxP3+ regulatory T cells colocalize with HSCs in the endosteal surface in the calvarial and trabecular BM, and this colocalization is lost after depletion of regulatory T cells.208 However, whether this interaction is biologically relevant to the homeostatic HSC maintenance is still unclear. Furthermore, regulatory T cells have been suggested to provide an immune-privileged niche in the BM, protecting HSCs from immune attacks.208

Neutrophils

Neutrophils are the most abundant subpopulation of leukocytes, with a BM reserve estimated to be 6 × 1011 in humans and 12 × 107 in mice.209,210 Neutrophils have short circulating half-life (6–8 h), after which they quickly migrate to tissues where they perform their functions.209 Serine proteases derived from neutrophils are capable of cleaving several cytokines and receptors essential for HSC retention in vitro, including CXCL12,211 CXCR4,212 VCAM-1,213 c-Kit,214 and SCF,215 suggesting that activated neutrophils create a proteolytic microenvironment that may contribute to HSC release from the BM. However, it was shown that, in mice lacking these proteases, G-CSF–induced HSC mobilization proceeds normally,216 suggesting that serine proteases are not essential for BM egress.

G-CSF induces neutrophil expansion in the BM, which may lead to MSC and osteoblast apoptosis and reductions in the expression of factors that are responsible for HSC retention in the BM.217 However, neutrophil number in the BM does not necessarily correlate with HSC mobilization.176 Another study showed that depleting circulating neutrophils increased CXCL12-expressing stromal cells number and CXCL12 protein levels, which resulted in enhanced retention of HSCs in the BM.184 Those effects are lost in mice in which neutrophils do not express CXCR4 and lack tropism to the BM, indicating that these effects may be exerted locally in the BM.184,218 Ablation of BM macrophages reverses the niche-modulating functions of neutrophils, indicating that the effect of neutrophils on the hematopoietic niche is dependent on macrophages. Together, these data explains how the daily clearance of aged neutrophils in the BM generates signals that affect HSCs in the BM niches.184

Conclusions and perspectives

The studies discussed in this review illustrate the contributions of multiple cell populations within the BM microenvironment to the complex regulation of HSC function. The use of sophisticated genetic tools has demonstrated that changes to the niche composition can have profound effects on HSC behavior. Recombination-based technology provides powerful means to interrogate the cellular and molecular components of niches. However, it is completely dependent on the promoter specificity and activity driving Cre, which must be characterized extensively before reaching conclusions about cell specificity or origins. Future clarification of the interactions between HSCs and their microenvironments during embryonic development may lead to improved methods to exploit the clinical potential of HSCs. Procedures to induce HSC self-renewal will benefit from this knowledge. Our increased understanding of healthy HSC niches should foster studies on the altered HSC niches in BM disorders. Targeting the niche itself is an attractive potential possibility for the treatment of hematologic disorders. The balance of extrinsic influences from the supportive niche may also vary under different physiological conditions. Newborn, adult, and aged HSCs have different physiological demands. The exploration of how the BM microenvironment ages will reveal essential information for the treatment of age-related BM illnesses. Likewise, understanding how the niche controls HSC function during stress situations, such as infections, radiotherapy, and chemotherapy, is needed. Recent studies suggest that BM endothelial cells play crucial roles in HSC recovery following radiation injury.89,219 A big challenge for the future will be to translate animal research into humans. Improving the availability of human tissue samples will be essential to reach this goal. Enormous advancement has been accomplished in our understanding of the importance and the complexity of the BM microenvironment to HSC function and to the health of the organism as a whole. The best is yet to come.

Figure 1
Diagram illustrating the quiescent arteriolar and the active sinusoidal HSC niches during BM homeostasis. The BM microenvironment hosts various hematopoietic and non-hematopoietic cell types, including macrophages, megakaryocytes, lymphocytes, neutrophils, ...

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