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Sci Rep. 2016; 6: 29007.
Published online 2016 July 1. doi:  10.1038/srep29007
PMCID: PMC4929501

Effects of neonatal stress on gamma oscillations in hippocampus

Abstract

Chronic early life stress increases adult risk for depression, bipolar disorder and schizophrenia, illnesses characterized by aberrant functions of cognition and memory. We asked whether chronic early life stress disrupts maturation of gamma oscillations, on which these functions depend. Lifelong impairment of the stress response results from separation of rat pups from the dam for three hours per day during a critical period of hippocampal development (PNDs 2–14). Parvalbumin-expressing interneurons, including the basket cell network which is fundamental to gamma oscillations, are reduced in number in post mortem studies of bipolar disorder and schizophrenia, and in chronically-stressed adult rats. To determine effects of chronic early life stress on gamma oscillations, we separated pups from dams once each day on PNDs 2–14 and recorded in vitro at PNDs 15–21. In control pups, separated for 15 minutes per day, gamma power had highly significant correlations with both age (p = 0.0022) and weight (p = 0.0024); gamma in pups separated for 180 minutes per day was not correlated with either factor. ANCOVA indicated significant differences between the groups in both measures. These findings indicate that chronic early life stress can disrupt maturation of the gamma oscillation network.

Chronic early-life stress has been shown in both animal and human studies to have a profound and lifelong impact on physical and psychological health, as correlated with increased rates of depression, schizophrenia, bipolar and anxiety-related disorders, alcohol and drug abuse and suicide, as well as cognitive and memory-related impairments1,2. Early-life adversity has been shown to impair in a virtually irreversible manner the negative-feedback system that normally returns circulating corticosteroids to basal levels in the wake of a stressor; the result is neurotoxic levels of exposure to corticosteroids beginning in the developing nervous system and continuing throughout the lifetime.

A well-established rearing protocol known as HMS180, which models chronic early-life adversity, has shown that separation of rat pups from their dam for three hours per day during a critical period of brain development, postnatal days 2 through 14, induces lifelong impairment of the stress response as a result of premature and excessive exposure to corticosteroids, with physiological and behavioral effects resembling those seen as a result of early-life trauma, neglect and abuse in human subjects3.

Postmortem studies of subjects with schizophrenia and bipolar disorder4,5, as well as studies of rats subjected to chronic stress in adulthood6,7, show significantly reduced numbers of parvalbumin-immunoreactive interneurons relative to controls. Approximately 60% of the parvalbumin-immunoreactive interneuron population in hippocampus is comprised of basket cells, interneurons which provide powerful perisomatic inhibition to pyramidal and granule cells; the rapid, synchronous firing of parvalbumin-expressing basket cells induces the rhythmic inhibitory postsynaptic potentials fundamental to gamma oscillations8,9. Gamma oscillations are intrinsic to cognition, perception and memory10,11 and aberrant in patients with schizophrenia and bipolar disorder12,13, illnesses thought to be linked to neurodevelopmental sequela in those genetically predisposed14,15, and for which risk is greatly increased by a history of chronic neglect, abuse or trauma in early life1.

In rat pups, PNDs 2 through 14 comprise the “stress hypo-response period” (SHRP), during which the stress response is, in effect, “offline”: under any less than extreme conditions, during the SHRP pups will mount little or no hormonal response to such aversive stimuli as surgery, handling, electric shock or extremes of temperature16. The dramatic increases in levels of adrenocorticotropin hormone and corticosterone that occur in response to a stressor before and after this phase are absent, and basal levels of circulating corticosterone fall markedly. Sapolsky and Meaney16 proposed that the SHRP is an evolutionary adaptation that serves to protect the nascent central nervous system from the catabolic effects on neural development of excessive exposure to glucocorticoids.

Sufficiently severe stress, however, such as that produced by a single 24-hour separation from the dam, can “break through” the SHRP to cause a significant rise in plasma corticosteroids. The negative-feedback system that turns off the release of glucocorticoids in the healthy adult is not fully functional until puberty17 so any exposure to glucocorticoids during the SHRP is extended, and the maturing feedback function commensurately impaired. While PVBCs in adult animals are known to be adversely affected by chronic stress, these cells may be especially vulnerable to developmental insult during the SHRP via chronic exposure to elevated levels of glucocorticoids, as well as during the protracted period of development that follows, given that they are still morphologically and electrophysiologically immature until PND2518.

Development of the neuroendocrine stress response is largely under maternal control in the rat: frequent licking and grooming of neonates by the dam is necessary for proper maturation of the hypothalamic-pituitary-adrenal axis. Chronic stress induced by low levels of licking and grooming during the critical period of HPA development induces epigenetic changes at a promoter region of the glucocorticoid receptor gene on or around postnatal day 8, resulting in the permanent down-regulation of glucocorticoid receptor expression in hippocampus19,20. As a result, the negative fast feedback signal is progressively degraded, so that following activation of the stress axis, glucocorticoids continue to be released for an extended time. Even after short-term stress, both adult and juvenile animals show altered dendritic morphology in pyramidal cells of the hippocampus and medial prefrontal cortex21, atrophy of pyramidal apical dendrites in hippocampus22 and decreased expression of synaptic spines23. Subsequently, lifelong exposure to elevated levels of glucocorticoids has been shown to have increasingly profound neurotoxic effects, including acceleration of normal age-related reduction of the glucocorticoid receptor population in hippocampus24, impairment of adult neurogenesis25, and acceleration of age-related loss of hippocampal volume due to dendritic atrophy and remodeling26.

Characteristically fast- spiking and non-accommodating, parvalbumin-expressing basket cells (PVBCs) form dense axonal arbors exclusively around the soma and proximal dendrites of pyramidal and granule cells, largely determining their output. Facilitating synchronization of PVBCs is that as a population they are networked via both chemical synapses and gap junctions. Widely thought to be the “timekeepers” of cortical network oscillations8, their precisely synchronized firing induces the inhibitory post-synaptic currents from pyramidal cells which constitute the field potential in cholinergically-induced gamma oscillations27.

While development of hippocampal pyramidal cells is largely complete at PND21, PVBCs require four to five postnatal weeks to become fully mature in their biophysical properties18. As the morphology, connectivity and intrinsic firing properties of PVBCs develop from slow- to fast-spiking, gamma oscillations increase in amplitude and frequency28. Duration of the action potential decreases by nearly half between PND5 and PND25, as does the decay constant of inhibitory post-synaptic currents, while the threshold for initiation of firing and maximal firing frequency increase. At maturity, each PVBC contacts about 60 other PVBCs and about 1,500 granule or pyramidal cells29.

Gamma oscillations have been recorded and described in vitro and in vivo in rodents of all ages, in a number of brain structures. However, the progressive maturation of hippocampal gamma oscillations, approximately spanning postnatal days 12 to 25 in rat pups, which occurs in parallel with and as a function of the morphological and electrophysiologcal maturation of PVBCs18, has not to our knowledge been documented with data points taken at time frames appropriate to the rapid changes occurring during this period, either in vivo or in vitro. In vitro studies of hippocampal gamma oscillations have in fact typically pooled data from animals between 14 and 22 days old27,30, in part, at least, because of the relative ease and consistency with which oscillations can be induced and maintained in the acute slice preparation during that phase, although in vivo studies using awake behaving, mature animals demonstrate that gamma oscillations continue to occur in the adult. The practice of pooling of data from animals which are still morphologically and electrophysiologically immature assumes that there are no significant age-related differences between 14- and 22-day old pups or between those pups and adults which would need to be considered in interpreting experimental results. This assumption is in conflict with what we now know about the electrophysiological maturation of cellular networks in hippocampus.

Impairment of parvalbumin-expressing interneuron networks in hippocampus or prefrontal cortex, by such mechanisms as increased asynchronous GABA release or loss of NMDA receptors31, disrupts gamma oscillations. Aberrant network activity caused by impairment of PVBC activity is widely thought to underlie the disturbances in mood, cognition and memory associated with mental illnesses32,33. PV+ interneurons have been shown to be reduced in prefrontal cortex and hippocampus in postmortem studies of human patients diagnosed with schizophrenia, major depression and bipolar disorder4,5, illnesses in which abnormal gamma oscillations are also observed34,35.

In linking impairment of PVBC networks in hippocampus and neocortex to psychiatric illness, questions still remain as to whether changes in this interneuron population precede the onset of psychiatric illness, or are a result of psychiatric illness or psychotropic medications, or are possibly a direct etiological factor in illness development. Studies of postmortem tissue14,15 indicated that the extent of reduction in the number of parvalbumin-immunoreactive cells in schizophrenia subjects was not correlated with age of the subject, duration or severity of their illness, their antipsychotic drug treatment history or incidence of drug abuse. These findings imply that the impairment of PVBC network function does not progress with the course or severity of the illness and is not a consequence of the use of psychotropic substances. These authors and others have proposed a neurodevelopmental origin for the deficit in function of parvalbumin-expressing interneurons, noting the relatively late postnatal onset of parvalbumin expression in contrast to other major neuronal calcium-binding proteins and that the parvalbumin-expressing interneuron population is the last to mature.

Given the sensitivity to perturbation of neuronal development during the SHRP, we asked whether gamma oscillations in rat pups would be affected by repeated episodes of maternal separation and concomitant premature exposure to glucocorticoids, and if so, whether that effect would be mediated by compromised development of the PVBC network.

Slice electrophysiology was utilized for this study because the slice preparation provides very tightly controllable and reproducible conditions ideal for this type of study; such variables as recording temperature and composition of the extracellular milieu are uniform for each experiment. Especially important to our study is that input to the slice is limited to that which is administered in the same manner each time in the form of pharmacological or electrophysiological manipulation, criteria impossible to achieve with in vivo designs. Isolated from the oscillatory activity of other brain regions, the variables affecting gamma activity can be defined in a simplified paradigm, and modified as needed. For these reasons, slice preparations have been very important in studies of the instrumental role of perisomatic inhibition by PVBCs in neuronal oscillations36,37,38,39.

Carbachol, an agonist of both muscarinic and nicotinic acetylcholinergic receptors, was chosen to induce gamma oscillations because it specifically mimics in vivo acetylcholine release from the dense cholinergic projection to the hippocampus from the medial septum/diagonal band of Broca40,41. In addition, carbachol produces oscillations having several of the most important features of gamma oscillations in vivo, including low frequency pyramidal cell firing which is phase-locked to the oscillation36. Carbachol has been preferentially employed to induce hippocampal gamma oscillations in vitro by many prominent investigators in this field27,30, including those whose methods have been cited in the present work, facilitating comparison of data from numerous labs.

As previously described42, each litter of pups was exposed to one of two rearing conditions from PNDs 2 through 14, inclusive: handling with separation from the dam for 15 minutes per day (HMS15) or handling with separation for 180 minutes per day (HMS180). After PND14, pups and dams in both groups were undisturbed except for standard institutional animal facility handling. In vitro electrophysiological studies were conducted on PNDs 15 through 21 on acute brain sections of dorsal hippocampus, using bath-applied carbachol (12.5 μM), a cholinergic receptor agonist, to induce gamma oscillations (Figs 1 and and2).2). Recordings were made from the pyramidal cell layer of CA3, distal to the fornix. Immunohistological staining of hippocampal tissue for parvalbumin (Fig. 3) was performed at the same longitudinal level of hippocampus.

Figure 1
Local field potentials become synchronous and oscillatory following introduction of AchR agonist.
Figure 2
Changes in power and frequency of oscillatory activity over time.
Figure 3
Maturation of PV+ interneuron network in dorsal hippocampus.

Methods and Experimental Design

All experiments were approved by the Animal Care and Use Committee at SUNY Downstate Medical Center and were in compliance with the guidelines of the institutional ethical code.

Culling and rearing of animals

Each group of pups was born in-house from three timed-pregnant Sprague-Dawley dams (Charles River, Kingston, NY). Dams were housed individually in adjacent polycarbonate cages with ad libitum access to food and water on a 12 hours: 12 hours light: dark cycle (lights on at 7:00 AM) and gave birth within close temporal proximity. The day of birth is referred to here as Postnatal Day 0 (PND 0). On PND1, 10–12 males and two females were placed with an unrelated dam; females were included in the litter to avoid single-sex litter effects36.

As previously described42, each foster litter was exposed to one of two rearing conditions from PNDs 2 through 14, inclusive: handling with separation from the dam for 15 minutes per day (HMS15) or handling with separation for 180 minutes per day (HMS180). For separation periods, pups were moved to an adjacent room and placed in a temperature- and humidity-controlled incubator (Lyon Technologies/Marsh Farms, Chula Vista, CA, Animal Intensive Care Unit with Digital Display). Separations were done at varying times during the light cycle (typically between 11am and 4pm) in order to increase stress on both dams and pups by limiting predictability. Other than the duration of separations from the dam, both HMS groups were treated in the same manner. After PND14, pups and dams in both groups were undisturbed except for standard institutional animal facility handling.

Electrophysiology

HMS-reared males aged 15–21 days were anesthetized with evaporating isoflurane and decapitated with a guillotine. The brain was quickly removed and submerged in ice-cold cutting solution (~4 °C) containing the following (in mM): sucrose, 252; KCl, 3; NaHCO3, 26; CaCl2, 1; MgSO4, 5; NaH2PO4, 1.25; glucose, 10, prepared with ultra pure water and bubbled with 95% O2/5% CO2 (carbogen gas)36. The brain was halved through the midline for use in electrophysiology and immunohistochemistry.

For electrophysiology studies, 400-μM thick slices of dorsal hippocampus were made using a Vibratome Series 3000 Plus (Ted Pella, Redding, CA). Slices were placed for ≥60 minutes on nylon netting submerged in room temperature extracellular solution of the following composition (in mM): NaCl, 126; KCl, 3; NaHCO3, 26; CaCl2, 1; MgSO4, 5; NaH2PO4, 1.25; glucose, 10, prepared with ultra pure water and bubbled with 95% O2/5% CO2 (carbogen gas)36.

Electrophysiology experiments were conducted using an Axon Multiclamp 700 A amplifier and an Axon Digidata 1332 A Digitizer (both, Molecular Devices, Sunnyvale, CA). Axon pClamp version 9.2 software (Molecular Devices) was used for data acquisition. Input was low pass filtered at 2 kHz and high pass filtered at 0.10 kHz prior to digitization, and sampled online at 6.667 kHz.

Slices were visualized using a Nikon Eclipse E600FN upright compound microscope with a 4X objective, Cool Snap ES2 High-Performance Interline CCD Camera and Nikon NIS Elements image acquisition software. Oscillations were visualized in real time on a spectrum analyzer using a DSO-2100 digital storage oscilloscope and software (Link Instruments, Fairfield, NJ) on a Gateway Solo 1400 notebook computer.

Recordings were made in a commercial submerged-type slice chamber (Warner Instruments Model RL-27L, Warner Instruments, Hamden, CT) using a Burleigh Piezoelectric micromanipulator (Thorlabs, Newton, NJ). Solution was maintained at 29.8–31.5 °C by an inline heater (Warner TC324B Temperature Controller, Warner Instruments, Hamden, CT), with a gravity-driven extracellular solution flow rate of 3.5–5.5 mL/min36.

Recording electrodes (3.5–5 MΩ) were pulled from thin-walled borosilicate glass capillaries, filled with the extracellular solution described above36 and placed in dorsal CA3, in the pyramidal cell layer and distal to the fimbria. Baseline recordings of local field potentials, three to seven minutes in duration, were made prior to addition of 12.5 μM carbachol (carbamoylcholine chloride) to the extracellular solution supply. Oscillations continued for as long as recording continued, which on some occasions exceeded three hours; oscillations could be rapidly terminated by 1 μM atropine, a competitive muscarinic receptor antagonist.

All chemicals and drugs for electrophysiology were purchased from Sigma-Aldrich except magnesium sulfate hydrate, purchased from Fluka Biochemika (Buchs, Switzerland), and sucrose, purchased from Fisher Scientific.

Data collection

Electrophysiological recordings were analyzed off-line using custom scripts with commercial software (Matlab 7.10, The MathWorks Inc., Natick, MA, 2000). In order to exclude harmonics of the gamma oscillations from power analysis, the bandwidth was filtered offline to a range of 20 to 40 Hz. Fundamental frequencies recorded during all experiments were within the 20 to 40 Hz band width prior to filtering. Analyses of gamma oscillations were in all cases performed on the 20-minute interval between 30 minutes and 50 minutes from the start of the recording. One brain slice was analyzed from each rat pup.

The gamma power data of one HMS180 pup was not used because it was 5.5 standard deviations above the mean of all HMS180 pups recorded in this study and 6 standard deviations above the mean of all HMS15 pups recorded in this study, exceeding our criteria of ≥2.5 standard deviations for determination of an outlier. Gamma oscillation frequency data from this pup, although within criteria, was also excluded as representative of the same recording. Naive rats of similar age and under the similar experimental conditions have produced oscillations of comparable gamma power in our lab (unpublished data) so we do not believe that investigator or equipment error accounted for this result.

Immunohistochemistry

The remaining half of the brain was submerged in a solution of paraformaldehyde (4% in PBS, Boston BioProducts, Ashland, MA) at 4 °C for 24 to 48 hours, stored in phosphate-buffered saline (PBS) at 4 °C, then sliced horizontally at a 40-μM thickness on a Leica VT 1200S Vibratome (Leica Microsystems, Buffalo Grove, IL). Sections used for immunohistochemistry were from the same longitudinal level of the hippocampus as those used for electrophysiology.

Sections were stored in PBS at 4 °C, washed three times for five minutes in fresh PBS, incubated for 60 minutes in 1% H2O2 in PBS, then washed three times for five minutes in PBS with 0.05% Tween-20 (PBST) (Acros Organics, Geel, Belgium). Sections were incubated for 60 minutes in 3% normal donkey serum (Jackson ImmunoResearch, West Grove, PA) and 0.5% Triton X-100 (Acros Organics, Geel, Belgium) in PBS, then for one hour at 4 °C with a monoclonal mouse anti-parvalbumin antibody specific to the calcium-bound form (Millipore MAB1572, dilution 1:3000) diluted in 3% normal donkey serum and 0.5% Triton X-100 in PBS. Sections were washed with PBST four times for five minutes, then incubated at room temperature for two hours with a biotinylated, rat-adsorbed horse anti-mouse IgG antibody (Abcam BA2001, dilution 1:100) diluted in 3% normal goat serum and 0.5% Triton X-100 in PBS and washed with PBST four times for five minutes.

Sections were then incubated with an avidin-biotin enzyme complex (ABC Vectastain Elite Standard Kit, Vector Laboratories, Burlingame, CA) for one hour and washed with PBST four times for five minutes each. DAB (3,3′-diaminobenzidine) (DAB-Plus Reagent Set, Novex, Life Sciences/Invitrogen, Grand Island, NY) was prepared with distilled H2O according to manufacturers’ directions, briefly centrifuged and pushed through a 22-μm filter by syringe. Sections were then incubated in filtered DAB for three to five minutes, washed in PBST and PBS three times for five minutes and then in distilled H2O for five minutes before being immersed in PBST for mounting on charged glass slides (Fisherbrand Superfrost Plus Microscope slides). ProLong Gold Antifade Reagent (Molecular Probes/Life Technologies) was applied to sections before coverslips were placed.

Whole-slide imaging was performed by New York University’s Office of Collaborative Science Experimental Pathology Histology Core Lab (http://ocs.med.nyu.edu/histopathology/contact-us) on a Leica SCN400F scanner at 40X magnification at a resolution of 0.250 microns per pixel. Native format image files were re-saved for use with ImageJ in tiff format with Aperio ImageScope v12.1.0.5029 (Leica Microsystems Inc., Buffalo Grove, IL).

Data collection

Boundaries of hippocampal subfields were defined on each section using guides from van Strien 200943. The Cell Counter plug-in feature in ImageJ was used to mark and tally cells manually identified by the investigator.

Data from each subfield of sections taken from the same animal were averaged. SEMs were calculated as the standard error of the mean of sections from the same pup. “Total PV+ cells” is the number of parvalbumin-labeled cells per hippocampal section, averaged over multiple sections from the same pup.

Immunohistological data was analyzed using Matlab 7.10 and ImageJ 1.49 g (Rasband, W.S., ImageJ, U.S. National Institutes of Health, Bethesda, Maryland, http://imagej.nih.gov/ij/, 1997–2012).

Statistical analysis

Data was normally distributed. Comparison of statistical models for analysis of HMS15 data indicated that it best fit a linear model, except for analyses involving gamma power, which best fit a semi-log model, with the X axis on a linear scale and the Y axis (gamma power) on a log scale. For this reason, gamma power data for both groups was transformed by using the square root of gamma power for all figures and analyses, by which both figures and analyses preferentially fit linear models. Parametric statistical analyses utilizing linear regression methods (Pearson correlation, multiple regression analysis and ANCOVA) were therefore employed throughout.

Figures were produced using Aperio ImageScope v12.1.0.5029, Matlab 7.10, Adobe Photoshop and Adobe Illustrator software versions CS5.5 and CC 2015.

Results

Maturation of gamma as predicted by age and weight

In agreement with previous studies of the electrophysiological maturation of fast-spiking interneurons in hippocampus in the neonate18, the power of gamma oscillations increased with age in the brief maternal separation (HMS15) group, with a highly significant coefficient of determination between those factors (R2 = 0.4280, p = 0.0024, n = 19) (Fig. 4a). In contrast, for the extended maternal separation (HMS180) pups, age would explain less than 2% of the variance in gamma power (R2 = 0.0171, p = 0.6049, n = 18). Analysis of covariance (ANCOVA) showed that the relationships between age and gamma power were significantly different in HMS15 versus HMS180 pups (p = 0.0342).

Figure 4
Correlations between measures of development and gamma oscillation power or frequency differ by rearing condition (brief vs. extended maternal separations).

The strength of the correlation between age and gamma power increased markedly across time in HMS15 pups. Between PNDs 15 and 18, the magnitude of gamma power in both HMS15 and HMS180 pups was not related to the factor of age (HMS15: R2 = 0.0198, p = 0.6996, n = 10; HMS180: R2 = 0.0880, p = 0.4045, n = 10). However, between PNDs 19 and 21, age predicted over 50% of the variance in gamma power in HMS15 pups (R2 = 0.5630, p = 0.0199, n = 9). ANCOVA showed that in HMS15 pups, the correlation between age and gamma power between postnatal days 15 and 18 differed significantly from that seen between postnatal days 19 and 21 (p = 0.0112). For HMS180 pups, the relationship between age and gamma power at PNDs 19 to 21 was relatively unchanged (R2 = 0.1209, p = 0.3979, n = 8).

Weight was a predictor of change in gamma power for HMS15 pups, explaining over 40% of the variance (R2 = 0.4321, p = 0.0022, n = 19) (Fig. 4b), in contrast to HMS180 animals, R2 = 0.0222 (p = 0.5553, n = 18), where no correlation was observed. ANCOVA indicated that HMS15 and HMS180 pups differed significantly in their respective correlations between weight and gamma power (p = 0.0388).

The strength of the correlation between weight and gamma power changed in both groups as they increased in size. At or below the median weight of 36 grams, the groups had similarly nominal and non-significant relationships between weight and gamma power (HMS15: R2 = 0.1285, p = 0.2797, n = 9; HMS180: R2 = 0.2604, p = 0.1604, n = 9). But in HMS15 pups weighing >36 grams, weight was a very strong and highly significant predictor of over 80% of gamma power variance (R2 = 0.8201, p = 0.0019, n = 10). ANCOVA showed that in HMS15 pups, the correlation between weight and gamma power between pups weighing ≤36 grams differed significantly from that in pups weighing >36 grams (p = 0.0388). For HMS180 pups >36 grams, the correlation between weight and gamma power (R2 = 0.0388, p = 0.6125, n = 9) was notably weaker than for pups at ≤36 grams.

HMS15 pups showed a significant correlation between gamma oscillation frequency and gamma power (R2 = 0.3255, p = 0.0107, n = 19) (Fig. 4c). In contrast, HMS180 pups showed virtually no relationship between those factors (R2 = 0.0008, p = 0.9125, n = 18).

Multiple regression analysis revealed a highly significant interaction between the factors of weight and gamma oscillation frequency in predicting gamma power in HMS15 pups, such that over 60% of the variance in gamma power could be explained by these two factors (multiple R2 = 0.6154, p = 0.0003, n = 19). Strong and significant interaction between age and oscillation frequency accounted for over 40% of gamma variance in HMS15 pups (multiple R2 = 0.4315, p = 0.0055, n = 19). In contrast, interaction between weight and oscillation frequency did not predict gamma power variance in HMS180 pups (multiple R2 = 0.0175, p = 0.8705, n = 18). Similarly weak interaction was seen in HMS180 pups between age and frequency (multiple R2 = 0.0149, p = 0.8705, n = 18).

Age was a weak predictor of gamma frequency in both HMS15 pups (R2 = 0.1840, p = 0.0666, n = 19), and HMS180 pups (R2 = 0.0578, p = 0.3369, n = 18) (Fig. 4d). Correlations between weight and frequency were also nominal for both groups (HMS15: R2 = 0.0789, p = 0.2428, n = 19; HMS180: R2 =  = 0.0704, p = 0.2876, n = 18).

Growth of PV+ interneuron population as predicted by weight

Correlations between age and the total number of parvalbumin-labeled cells per pup were moderate and significant for HMS15 pups (R2 = 0.2880, p = 0.0218, n = 18) but weak for HMS180 pups (R2 = 0.1480, p = 0.0846, n = 21) (Fig. 5a). Correlations in HMS groups between the number of parvalbumin-labeled cells and age also differed in CA2/3, with a moderate but significant correlation in HMS15 pups (R2 = 0.2626, p = 0.0297, n = 18) and nominal correlation in HMS180 pups (R2 = 0.1329, p = 0.1042, n = 21) (Fig. 5c). No significant correlations were observed in either of the groups between age and the number of parvalbumin-labeled cells in dentate gyrus (HMS15: R2 = 0.0757, p = 0.2692, n = 18; HMS180: R2 = 0.0412, p = 0.3777, n = 21) (Fig. 5b) or CA1 (HMS15: R2 = 0.1431, p = 0.1217, n = 18; HMS180: R2 = 0.1561, p = 0.0763, n = 21) (Fig. 5d).

Figure 5
Effect of rearing condition (brief vs. extended maternal separations) on relationships between age (a–d) or weight (e–h) and number of PV+ cells in hippocampus.

Weight was a much stronger explanatory factor, predicting the total number of labeled cells (Fig. 5e: HMS15: R2 = 0.4805, p = 0.0053, n = 18; HMS180: R2 = 0.2911, p = 0.0117, n = 21), the number of stained cells in CA2/3 (Fig. 5g, HMS15: R2 = 0.3638, p = 0.0081, n = 18; HMS180: R2 = 0.2274, p = 0.0288, n = 21) and in CA1 (Fig. 5h, HMS15: R2 = 0.2244, p = 0.0437, n = 18; HMS180: R2 = 0.2301, p = 0.0228, n = 21) in a strong and significant manner for both HMS groups. In dentate gyrus, HMS15 pups showed a modest correlation between weight and number of parvalbumin-stained cells (R2 = 0.2220, p = 0.0484, n = 18), while HMS180 pups showed a weaker and non-significant correlation (R2 = 0.1424, p = 0.0917, n = 21) (Fig. 5f).

Maturation of gamma as predicted by subfields’ PV+ populations

The number of PV+ cells in CA1 was a strong predictor of gamma power in HMS15 pups (R2 = 0.4654, p = 0.0207, n = 11) but not in HMS180 pups (R2 = 0.1168, p = 0.2317, n = 14) (Fig. 6d). A significant relationship was also seen in HMS15 pups between gamma power and total number of parvalbumin-stained cells per pup (R2 = 0.4130, p = 0.0330, n = 11; HMS180: R2 = 0.1284, p = 0.2084, n = 14) (Fig. 6a). No other strong relationships between gamma power and number of stained cells were observed. Correlations were non-significant in both groups in dentate gyrus (Fig. 6b, HMS15: R2 = 0.2816, p = 0.0931, n = 11; HMS180: R2 = 0.1266, p = 0.2118, n = 14) and CA2/3 (Fig. 6c, HMS15: R2 = 0.2106, p = 0.1557, n = 11; HMS180: R2 = 0.0455, p = 0.4639, n = 14).

Figure 6
Subfield-specific effect of rearing condition (brief vs. extended maternal separations) on relationship between number of PV+ cells and gamma oscillation power (a–d) or frequency (e–h).

The relationship between the total number of parvalbumin-stained cells per pup and gamma frequency was non-significant for both HMS groups (Fig. 6e, HMS15: R2 = 0.2974, p = 0.0830, n = 11; HMS180: R2 = 0.1777, p = 0.1334, n = 14). In HMS15 pups, however, the correlation between gamma frequency and number of immunostained cells specifically in dentate gyrus was very strong and highly significant (Fig. 6f, R2 = 0.6761, p = 0.0019, n = 11; HMS180 R2 = 0.0706, p = 0.3609, n = 14). The number of stained cells was not predictive of gamma frequency in either group in CA2/3 (Fig. 6g, HMS15: R2 = 0.0880, p = 0.3766, n = 11; HMS180: R2 = 0.1630, p = 0.1519, n = 14) or in CA1 (Fig. 6h, HMS15: R2 = 0.1069, p = 0.3255, n = 11; HMS180: R2 = 0.0572, p = 0.4120, n = 14; CA2/3: R2 = 0.1630, p = 0.1519, n = 14).

The relationship between age and weight was not differentially affected by HMS15 and HMS180 rearing (HMS15: R2 = 0.7273, n = 26, HMS180: R2 = 0.8517, n = 26, both p < 0.0001, figures not shown). No differences were noted in pups’ or dams’ appearance or behavior.

HMS180 rearing affects temporal progress of electrophysiological maturation

Physiological development over time has been taken into account in our statistical analyses by looking at correlations of gamma power, gamma frequency and number of parvalbumin-immunopositive cells with age or weight. However, it is interesting to note that when described by simple averages of a seven-day period, very little difference was seen between the groups (Table 1). Pooling of the data by group across a seven day period, irregardless of age or weight, indicates that the duration of maternal separations, either 15 or 180 minutes, did not differentially affect the pups. Differences between the groups only emerge when the electrophysiological data is placed into context as a description of a developmental process, in conjunction with a critical variable representing progressive measures of maturity —herein represented by age or weight —and data points plotted at appropriately spaced intervals, i.e., 24 hours. Outside of this context, aberrance in the developing gamma oscillation network in HMS180 pups compared to the HMS15 pups, as shown by the preceding findings, is effectively masked.

Table 1
Effects of rearing condition (brief vs extended maternal separations) on 7-day averages of somatic and electrophysiological maturation.

Discussion

Our findings indicate that neonatal maturation of hippocampal gamma oscillations is profoundly affected by chronic stress in the form of extended separations from the dam. Gamma oscillation power and frequency were significantly correlated with both age and weight in HMS15 pups but not in HMS180 pups. These strong correlations in HMS15 pups emerged only at later stages of maturation within the seven days of our study: at higher weights (>36 grams versus ≤36 grams) or later age (postnatal days 19 to 21 versus postnatal days 15 to 18); correlations were nominal in both groups prior to that, and in HMS180 pups remained so. This suggests that HMS180 rearing interferes with a critical phase of electrophysiological network maturation which is normally initiated during the third week of postnatal life.

PVBCs are last cells in the hippocampus to reach adult form and thus extremely vulnerable to the catabolic effects of premature exposure to glucocorticoids during the maternal separations phase of our study, PNDs 2 through 14; excessive exposure to glucocorticoids continues in HMS180 pups even after the stress hypo-response period closes. Developmental defects thus may rise at multiple levels, and HMS180 rearing may specifically affect gamma power and frequency in many ways. The complex arbor-like morphology of parvalbumin-expressing basket cells requires four to five weeks to complete, for example, as does the consequent electrophysiological maturation of the PVBC network. Aberrant calcium buffering at the axon terminal due to abnormalities in expression levels of parvalbumin would cause major changes in the firing and recovery properties of these cells, as would changes in passive membrane properties or synaptic connectivity as a function of anomalous cellular morphology. Relevant to the age of pups in our study as well is that increased oscillation frequency capability is in large part a function of changes in subunit expression both pre-and post-synaptically. The Kv3.2 subunit of the voltage-gated potassium channel, found predominantly on GABAergic fast-firing cells including parvalbumin-expressing interneurons44, facilitates recovery of sodium channels from inactivation and reduces the duration of after-hyperpolarization. By minimizing time in the refractory state, potassium channels having the Kv3.2 subunit facilitate high-frequency firing including gamma oscillations45. In hippocampus, Kv3.2 expression is seen predominantly in the pyramidal cell layer of CA1 and CA2/3 and at high levels in the hilar region of dentate gyrus45. Expression reaches adult levels by PND2144. In addition, the alpha-1 subunit of the GABA-A receptor is enriched at the postsynaptic side of synapses between parvalbumin-expressing basket cells, facilitating precisely synchronized activity in the basket cell network. The presence of this subunit in GABA-A receptors has been shown to facilitate faster activation and deactivation of those receptors in mature rats46. In immature rat hippocampus, alpha-1 subunits are present only in small number, but their expression increases greatly during the second and third weeks of postnatal life47. Increased expression of these subunits is also associated with developmental maturation of parvalbumin-expressing basket cell kinetics. Thus the irregular progression of gamma power expression seen in HMS180 pups may represent impairments of a variety of critical-period-dependent maturation processes.

Of the parvalbumin-immunopositive population seen in the HMS180s, cells that are not morphologically, neurochemically or electrophysiologically sound may not survive. This would align our findings with post-mortem studies that have found a deficit in parvalbumin-immunoreactivity in hippocampus and cortex in adults in the wake of schizophrenia, bipolar disorder or chronic stress4,5. Aberrant cells are more likely to atrophy and die off, or they may even change phenotype, as resulted from neonatal oxidative stress in a schizophrenia model, which in a recent study caused parvalbumin expression in GFP-labeled PVBCs to fall below detectable levels by or before the age of five weeks48. Similarly, work with post-mortem tissue from adult schizophrenic patients, in which PVBCs were identified by traits other than parvalbumin-immunoreactivity49, showed no difference from controls in the number of cells or density of GABAergic presynapatic PVBC axon terminals but did reveal significantly lower levels of parvalbumin mRNA. What has not been established with regard to schizophrenia or bipolar disorder is at what point in the lifetime these changes in PVBCs occur. Chronic early-life stress as modeled in this study is known to be a significant predictor of increased risk for both illnesses1,2,3,50,51,52.

Studies using the HMS180 protocol employed here have shown that HMS15-reared pups and adults are comparable in their behavior, cognitive abilities and neuroendocrine functions to naive animal facility-reared animals53,54. Results of our study show that during critical periods of neural development, up to and perhaps including the juvenile phase, factors which are potentially correlated with age and/or weight should be assessed before any degree of “homogeneity” in the group is assumed. Our findings suggest that the developmental trajectory of gamma oscillation power in naive pups is sufficiently steep between PNDs 15 and 21 that “pooling” their electrophysiological data could produce misleading results. This would be borne out not only by our findings but also by studies which have documented the electrophysiological and morphological maturation process of parvalbumin-expressing basket cells at time points between PNDs 6 and 25, illustrating the extraordinary transformation of these cells from “slow to fast signaling devices” within this very brief time18.

We conclude that HMS180 rearing, an animal model for the effects in humans of chronic early-life abuse, trauma or neglect, disrupts the developmental trajectory of the parvalbumin-expressing basket cell network and consequently impairs maturation of gamma oscillation activity in hippocampus. Impaired maturation of gamma oscillations may be a fundamental link between cognitive and memory deficits associated with chronic life stress and the etiologies of schizophrenia and bipolar disorder, which are thought to have bases in aberrant neurodevelopment, and for which those with histories of chronic early-life stress are at significantly increased risk.

Additional Information

How to cite this article: Dricks, S. Effects of neonatal stress on gamma oscillations in hippocampus. Sci. Rep. 6, 29007; doi: 10.1038/srep29007 (2016).

References

  • McEwen B. S. Protection and damage from acute and chronic stress: allostasis and allostatic overload and relevance to the pathophysiology of psychiatric disorders. Annals of the New York Academy of Sciences 1032, 1–7 (2004). [PubMed]
  • Heim C., Newport D. J., Mletzko T., Miller A. H. & Nemeroff C. B. The link between childhood trauma and depression: insights from HPA axis studies in humans. Psychoneuroendocrinology 33, 693–710 (2008). [PubMed]
  • Sanchez M. M. et al. . Effects of sex and early maternal abuse on adrenocorticotropin hormone and cortisol responses to the corticotropin-releasing hormone challenge during the first 3 years of life in group-living rhesus monkeys. Dev. Psychopathol. 22, 45–53 (2010). [PMC free article] [PubMed]
  • Knable M. B. et al. . Molecular abnormalities of the hippocampus in severe psychiatric illness: postmortem findings from the Stanley Neuropathology Consortium. Mol Psychiatry 9, 609–20–544 (2004). [PubMed]
  • Sakai T. et al. . Changes in density of calcium-binding-protein-immunoreactive GABAergic neurons in prefrontal cortex in schizophrenia and bipolar disorder. Neuropathology 28, 143–150 (2008). [PubMed]
  • Czéh B. et al. . Chronic stress decreases the number of parvalbumin-immunoreactive interneurons in the hippocampus: prevention by treatment with a substance P receptor (NK1) antagonist. Neuropsychopharmacology 30, 67–79 (2005). [PubMed]
  • Hu W., Zhang M., Czéh B., Flügge G. & Zhang W. Stress impairs GABAergic network function in the hippocampus by activating nongenomic glucocorticoid receptors and affecting the integrity of the parvalbumin-expressing neuronal network. Neuropsychopharmacology 35, 1693–1707 (2010). [PMC free article] [PubMed]
  • Mann E. O. & Paulsen O. Mechanisms underlying gamma (‘40 Hz’) network oscillations in the hippocampus–a mini-review. Prog Biophys Mol Biol. 87, 67–76 (2005). [PubMed]
  • Hajos N. & Paulsen O. Network mechanisms of gamma oscillations in the CA3 region of the hippocampus. Neural Netw. 22, 1113–1119 (2009). [PubMed]
  • Kahana M. J. The cognitive correlates of human brain oscillations. Journal of Neuroscience 26, 1669–1672 (2006). [PubMed]
  • Jensen O., Kaiser J. & Lachaux J.-P. Human gamma-frequency oscillations associated with attention and memory. Trends Neurosci. 30, 317–324 (2007). [PubMed]
  • Lewis D. A., Hashimoto T. & Volk D. W. Cortical inhibitory neurons and schizophrenia. Nat Rev Neurosci. 6, 312–324 (2005). [PubMed]
  • Volman V., Behrens M. M. & Sejnowski T. J. Downregulation of parvalbumin at cortical GABA synapses reduces network gamma oscillatory activity. Journal of Neuroscience 31, 18137–18148 (2011). [PMC free article] [PubMed]
  • Zhang Z. J. & Reynolds G. P. A selective decrease in the relative density of parvalbumin-immunoreactive neurons in the hippocampus in schizophrenia. Schizophr. Res. 55, 1–10 (2002). [PubMed]
  • Reynolds G. P., Zhang Z. J. & Beasley C. L. Neurochemical correlates of cortical GABAergic deficits in schizophrenia: selective losses of calcium binding protein immunoreactivity. Brain Res Bull 55, 579–584 (2001). [PubMed]
  • Sapolsky R. M. & Meaney M. J. Maturation of the adrenocortical stress response: neuroendocrine control mechanisms and the stress hyporesponsive period. Brain Research 396, 64–76 (1986). [PubMed]
  • Walker C. D., Perrin M., Vale W. & Rivier C. Ontogeny of the Stress Response in the Rat: Role of the Pituitary and the Hypothalamus*. Endocrinology 118, 1445–1451 (1986). [PubMed]
  • Doischer D. et al. . Postnatal differentiation of basket cells from slow to fast signaling devices. J Neurosci. 28, 12956–12968 (2008). [PubMed]
  • Fish E. W. et al. . Epigenetic programming of stress responses through variations in maternal care. Annals of the New York Academy of Sciences 1036, 167–180 (2004). [PubMed]
  • Szyf M., Weaver I. C. G., Champagne F. A., Diorio J. & Meaney M. J. Maternal programming of steroid receptor expression and phenotype through DNA methylation in the rat. Front Neuroendocrinol0. 26, 139–162 (2005). [PubMed]
  • McEwen B. S. Glucocorticoids, depression, and mood disorders: structural remodeling in the brain. Metabolism 54, 20–23 (2005). [PubMed]
  • McEwen B. S. & Magarinos A. Stress and hippocampal plasticity: implications for the pathophysiology of affective disorders. Hum Psychopharmacol. 16, S7–S19 (2001). [PubMed]
  • Silva-Gómez A. Decreased dendritic spine density on prefrontal cortical and hippocampal pyramidal neurons in postweaning social isolation rats. Brain Research 983, 128–136 (2003). [PubMed]
  • Hibberd C., Yau J. L. & Seckl J. R. Glucocorticoids and the ageing hippocampus. Journal of anatomy 197, 553–562 (2002). [PubMed]
  • Xi G. et al. . Fluoxetine attenuates the inhibitory effect of glucocorticoid hormones on neurogenesis in vitro via a two-pore domain potassium channel, TREK-1. Psychopharmacology 214, 747–759 (2011). [PubMed]
  • McEwen B. S. Sex, stress and the hippocampus: allostasis, allostatic load and the aging process. Neurobiol Aging 23, 921–939 (2002). [PubMed]
  • Gulyás A. I. et al. . Parvalbumin-containing fast-spiking basket cells generate the field potential oscillations induced by cholinergic receptor activation in the hippocampus. Journal of Neuroscience 30, 15134–15145 (2010). [PMC free article] [PubMed]
  • Lahtinen H. et al. . Postnatal development of rat hippocampal gamma rhythm in vivo. J Neurophysiol 88, 1469–1474 (2002). [PubMed]
  • Sik A., Penttonen M., Ylinen A. & Buzsaki G. Hippocampal CA1 interneurons: an in vivo intracellular labeling study. J Neurosci. 15, 6651–6665 (1995). [PubMed]
  • Oren I., Mann E. O., Paulsen O. & Hajos N. Synaptic currents in anatomically identified CA3 neurons during hippocampal gamma oscillations in vitro. J Neurosci. 26, 9923–9934 (2006). [PubMed]
  • Carlén M. et al. . A critical role for NMDA receptors in parvalbumin interneurons for gamma rhythm induction and behavior. Mol Psychiatry 17, 537–548 (2012). [PMC free article] [PubMed]
  • Nakazawa K. et al. . GABAergic interneuron origin of schizophrenia pathophysiology. Neuropharmacology 62, 1574–1583 (2012). [PMC free article] [PubMed]
  • Light G. A. et al. . Gamma band oscillations reveal neural network cortical coherence dysfunction in schizophrenia patients. Biol Psychiatry 60, 1231–1240 (2006). [PubMed]
  • Özerdem A., Güntekin B., Atagün I., Turp B. & Başar E. Reduced long distance gamma (28–48 Hz) coherence in euthymic patients with bipolar disorder. J Affect Disord 132, 325–332 (2011). [PubMed]
  • Liu T.-Y. et al. . Different patterns of abnormal gamma oscillatory activity in unipolar and bipolar disorder patients during an implicit emotion task. Neuropsychologia 50, 1514–1520 (2012). [PubMed]
  • Hajos N. et al. . Spike timing of distinct types of GABAergic interneuron during hippocampal gamma oscillations in vitro. J Neurosci. 24, 9127–9137 (2004). [PubMed]
  • Pálhalmi J., Paulsen O., Freund T. F. & Hajos N. Distinct properties of carbachol- and DHPG-induced network oscillations in hippocampal slices. Neuropharmacology 47, 381–389 (2004). [PubMed]
  • Hajos N. et al. . Maintaining network activity in submerged hippocampal slices: importance of oxygen supply. Eur J Neurosci. 29, 319–327 (2009). [PMC free article] [PubMed]
  • Oren I., Hajos N. & Paulsen O. Identification of the current generator underlying cholinergically induced gamma frequency field potential oscillations in the hippocampal CA3 region. J Physiol. 588, 785–797 (2010). [PubMed]
  • Williams J. H. & Kauer J. A. Properties of carbachol-induced oscillatory activity in rat hippocampus. J Neurophysiol. 78, 2631–2640 (1997). [PubMed]
  • Teles-Grilo Ruivo L. M. & Mellor J. R. Cholinergic modulation of hippocampal network function. Front. Syna. Neurosci. 5, 2 (2013). [PMC free article] [PubMed]
  • Ladd C. O., Huot R. L., Thrivikraman K. V., Nemeroff C. B. & Plotsky P. M. Long-term adaptations in glucocorticoid receptor and mineralocorticoid receptor mRNA and negative feedback on the hypothalamo-pituitary-adrenal axis following neonatal maternal separation. Biol Psychiatry 55, 367–375 (2004). [PubMed]
  • van Strien N. M., Cappaert N. L. M. & Witter M. P. The anatomy of memory: an interactive overview of the parahippocampal-hippocampal network. Nat Rev Neurosci. 10, 272–282 (2009). [PubMed]
  • Tansey E. P., Chow A., Rudy B. & McBain C. J. Developmental expression of potassium-channel subunit Kv3.2 within subpopulations of mouse hippocampal inhibitory interneurons. Hippocampus 12, 137–148 (2002). [PubMed]
  • Atzori M. et al. . H2 histamine receptor-phosphorylation of Kv3.2 modulates interneuron fast spiking. Nat Neurosci. 3, 791–798 (2000). [PubMed]
  • Lavoie A. M., Tingey J. J., Harrison N. L., Pritchett D. B. & Twyman R. E. Activation and deactivation rates of recombinant GABA(A) receptor channels are dependent on alpha-subunit isoform. Biophys. J. 73, 2518–2526 (1997). [PubMed]
  • Laurie D. J., Wisden W. & Seeburg P. H. The distribution of thirteen GABAA receptor subunit mRNAs in the rat brain. III. Embryonic and postnatal development. J Neurosci. 12, 4151–4172 (1992). [PubMed]
  • Powell S. B., Sejnowski T. J. & Behrens M. M. Behavioral and neurochemical consequences of cortical oxidative stress on parvalbumin-interneuron maturation in rodent models of schizophrenia. Neuropharmacology 62, 1322–1331 (2012). [PMC free article] [PubMed]
  • Glausier J. R., Fish K. N. & Lewis D. A. Altered parvalbumin basket cell inputs in the dorsolateral prefrontal cortex of schizophrenia subjects. Mol Psychiatry 19, 30–36 (2014). [PMC free article] [PubMed]
  • Molnar B. E., Buka S. L. & Kessler R. C. Child sexual abuse and subsequent psychopathology: results from the National Comorbidity Survey. Am J Public Health 91, 753–760 (2001). [PubMed]
  • Heim C. & Nemeroff C. B. The role of childhood trauma in the neurobiology of mood and anxiety disorders: preclinical and clinical studies. Biol Psychiatry 49, 1023–1039 (2001). [PubMed]
  • Wolff N. & Shi J. Childhood and adult trauma experiences of incarcerated persons and their relationship to adult behavioral health problems and treatment. Int J Environ Res Public Health 9, 1908–1926 (2012). [PMC free article] [PubMed]
  • Gutrndn D. A. & Nemerofi, C. B. Neurobiology of Early Life Stress: Rodent Studies. Semin Clin Neuropsychiatry 7, 89–95 (2002). [PubMed]
  • Plotsky P. M. et al. . Long-term consequences of neonatal rearing on central corticotropin-releasing factor systems in adult male rat offspring. Neuropsychopharmacology 30, 2192–2204 (2005). [PubMed]

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