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Genome Announc. 2016 May-Jun; 4(3): e00611-16.
Published online 2016 June 23. doi:  10.1128/genomeA.00611-16
PMCID: PMC4919414

Draft Genome Sequence of Shewanella baltica M1 Isolated from Brackish Surface Water of the Gulf of Gdańsk


Here, we present the 5.168-Mbp draft genome sequence of Shewanella baltica M1, the first Shewanella strain from the Gulf of Gdańsk to have its genome sequenced and annotated. The availability of the genome sequence of strain M1 will promote further global analyses of bacterial stress responses in the unique Gulf of Gdańsk ecosystem.


Shewanella baltica M1 was isolated in summer 2005 from surface water of the Gulf of Gdańsk (54°33′01.06″N, 18°39′45.50″E), a southeastern bay of the Baltic Sea. Water in this unique basin is subject to substantial hydrological and hydrochemical variability, which results from large anthropogenic impact, dynamic interactions of marine and fresh water, and the morphometry of the Gulf of Gdańsk itself (1). For instance, while the average surface salinity of the Gulf of Gdańsk is about 7 PSU, it periodically drops to around 1 PSU in nearshore waters (2). These varied natural and anthropogenic impacts affect the functioning of the unique Gulf of Gdańsk ecosystem (3).

The genus Shewanella comprises a phenotypically diverse group of bacteria with a worldwide distribution (4). S. baltica strains occupy primarily aquatic and sedimentary niches that are chemically stratified on a permanent or seasonal basis (5). They were also shown to be the major contributors—among the H2S-producing species—to the spoilage of iced marine fish (6). To successfully compete in such varied environments and efficiently respond to the niche-specific resources, S. baltica has developed robust respiratory, metabolic, sensing, and regulatory systems (7). Here, we report the draft genome sequence of strain M1, the first Shewanella strain from the Gulf of Gdańsk to have its genome sequenced. The strain was identified based on matrix-assisted laser desorption ionization–time of flight mass spectrometry profiling, confirmed by the whole-genome sequencing reported here.

Whole-genomic DNA was purified with the use of the Sigma GenElute bacterial genomic DNA kit from a fresh overnight culture. Illumina paired-end libraries were prepared with 1 ng of genomic DNA using the Nextera XT kit. A total of 9,591,472 paired-end reads were generated on the Illumina NextSeq500 platform at a read length of 2 × 150 bp. Reads were analyzed and quality-checked using FastQC version 0.11.13 ( Low-quality data were filtered such that, for a pair of paired-end reads, each read had more than 90% of bases with a quality score greater than or equal to Q20. Genome assembly was performed using SPAdes version 3.7.1 (8), combing de novo assembly and manual editing. The final assembly consisted of 91 contigs (>500 bp) totaling 5,167,578 bp, with a GC content of 46.16 mol% and an average genome coverage of 34×. The N50 and N75 contig lengths were 143,280 bp and 76,941 bp, respectively.

The closest whole-genome sequences are those of S. baltica OS625 (85.84% symmetrical identity and 96.75% gapped identity) and S. baltica OS185 (84.94% symmetrical identity and 96.78% gapped identity) (9) among the draft and the complete genome sequences, respectively, in NCBI.

The draft genome of strain M1 was annotated using the Prokaryotic Genome Annotation Pipeline version 3.1 at NCBI (10). The annotation revealed 4,270 predicted protein-coding sequences, 92 tRNA genes, and 14 rRNA operons.

The availability of the genome sequence of S. baltica M1 will facilitate further transcriptome- and proteome-centric approaches to explain how versatile Shewanella strains functionally interact with their habitats.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number LWED00000000. The version described in this paper is the first version, LWED01000000.


Citation Karczewska-Golec J, Strapagiel D, Sadowska M, Szalewska-Pałasz A, Golec P. 2016. Draft genome sequence of Shewanella baltica M1 isolated from brackish surface water of the Gulf of Gdańsk. Genome Announc 4(3):e00611-16. doi:10.1128/genomeA.00611-16.


1. Nowacki J, Jarosz E 1998. The hydrological and hydrochemical division of the surface waters in the gulf of Gdańsk. Oceanologia 40:261–272.
2. Kruk-Dowgiałło L, Szaniawska A 2008. Gulf of Gdańsk and Puck Bay, p 139–165. In Schiewer U (ed), Ecology of Baltic coastal waters, Ecological studies, vol 197. Springer, Berlin.
3. Tomczak MT, Szymanek L, Pastuszak M, Grygiel W, Zalewski M, Gromisz S, Ameryk A, Kownacka J, Psuty I, Kuzebski E, Grzebielec R, Margoński P 2016. Evaluation of trends and changes in the Gulf of Gdańsk ecosystem—an integrated approach. Estuaries Coasts 39:593–604 doi:.10.1007/s12237-015-0026-4 [Cross Ref]
4. Nealson KH, Scott J 2006. Ecophysiology of the genus Shewanella, p. 1133–1151. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (ed), The prokaryotes: a handbook on the biology of bacteria, vol 7, 3rd ed. Springer, New York.
5. Caro-Quintero A, Deng J, Auchtung J, Brettar I, Höfle MG, Klappenbach J, Konstantinidis KT 2011. Unprecedented levels of horizontal gene transfer among spatially co-occurring Shewanella bacteria from the Baltic sea. ISME J 5:131–140. doi:.10.1038/ismej.2010.93 [PMC free article] [PubMed] [Cross Ref]
6. Fonnesbech Vogel BF, Venkateswaran K, Satomi M, Gram L 2005. Identification of Shewanella baltica as the most important H2S-producing species during iced storage of Danish marine fish. Appl Environ Microbiol 71:6689–6697. doi:.10.1128/AEM.71.11.6689-6697.2005 [PMC free article] [PubMed] [Cross Ref]
7. Fredrickson JK, Romine MF, Beliaev AS, Auchtung JM, Driscoll ME, Gardner TS, Nealson KH, Osterman AL, Pinchuk G, Reed JL, Rodionov DA, Rodrigues JLM, Saffarini DA, Serres MH, Spormann AM, Zhulin IB, Tiedje JM 2008. Towards environmental systems biology of Shewanella. Nat Rev Microbiol 6:592–603. doi:.10.1038/nrmicro1947 [PubMed] [Cross Ref]
8. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:.10.1089/cmb.2012.0021 [PMC free article] [PubMed] [Cross Ref]
9. Varghese NJ, Mukherjee S, Ivanova N, Konstantinidis KT, Mavrommatis K, Kyrpides NC, Pati A 2015. Microbial species delineation using whole genome sequences. Nucleic Acids Res 43:6761–6771. doi:.10.1093/nar/gkv657 [PMC free article] [PubMed] [Cross Ref]
10. Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D, Garrity G, Kodira CD, Kyrpides N, Madupu R, Markowitz V, Tatusova T, Thomson N, White O 2008. Toward an online repository of standard operating procedures (SOPs) for (meta)genomic annotation. Omics 12:137–141. doi:.10.1089/omi.2008.0017 [PMC free article] [PubMed] [Cross Ref]

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