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Conceived and designed the experiments: OSR CBe SC SG SR. Performed the experiments: YFG JR CBu GW SR. Analyzed the data: YFG JR GW SR. Contributed reagents/materials/analysis tools: JR GW. Wrote the paper: SG SR.
Andes virus (ANDV) is a human-pathogenic hantavirus. Hantaviruses presumably initiate their mRNA synthesis by using cap structures derived from host cell mRNAs, a mechanism called cap-snatching. A signature for a cap-snatching endonuclease is present in the N terminus of hantavirus L proteins. In this study, we aimed to solve the atomic structure of the ANDV endonuclease and characterize its biochemical features. However, the wild-type protein was refractory to expression in Escherichia coli, presumably due to toxic enzyme activity. To circumvent this problem, we introduced attenuating mutations in the domain that were previously shown to enhance L protein expression in mammalian cells. Using this approach, 13 mutant proteins encompassing ANDV L protein residues 1–200 were successfully expressed and purified. Protein stability and nuclease activity of the mutants was analyzed and the crystal structure of one mutant was solved to a resolution of 2.4 Å. Shape in solution was determined by small angle X-ray scattering. The ANDV endonuclease showed structural similarities to related enzymes of orthobunya-, arena-, and orthomyxoviruses, but also differences such as elongated shape and positively charged patches surrounding the active site. The enzyme was dependent on manganese, which is bound to the active site, most efficiently cleaved single-stranded RNA substrates, did not cleave DNA, and could be inhibited by known endonuclease inhibitors. The atomic structure in conjunction with stability and activity data for the 13 mutant enzymes facilitated inference of structure–function relationships in the protein. In conclusion, we solved the structure of a hantavirus cap-snatching endonuclease, elucidated its catalytic properties, and present a highly active mutant form, which allows for inhibitor screening.
Hantaviruses may cause severe disease in humans, either hemorrhagic fever or cardiopulmonary syndrome. Both conditions are associated with high lethality. Vaccines and effective treatments approved for application in humans are not available. The L protein plays a central role in hantavirus replication and represents a key target for antiviral drug development. However, the protein is difficult to study, as it can hardly be expressed in cells, and therefore structure and function are largely unknown. We succeeded to express and purify the N-terminal domain of ANDV, a South American hantavirus, in mutant forms. The atomic structure of the domain was solved by X-ray crystallography. The structural data in conjunction with our enzymatic activity data demonstrate that the N-terminal part of ANDV L protein harbors an endonuclease that cleaves RNA, presumably to “steal” cap structures from cellular mRNA for virus transcription. The investigation of a range of mutant enzymes revealed the detailed role of amino acid residues within the endonuclease. Importantly, using a known inhibitor of viral endonucleases we demonstrate the potential of the ANDV enzyme as an antiviral target. The discovery of a highly active mutant form of the endonuclease, which can be expressed at high level, will facilitate drug discovery programs for hantaviruses.
Andes virus (ANDV) belongs to the genus Hantavirus within the family Bunyaviridae. Hantaviruses can be pathogenic to humans and are distributed worldwide [1–3]. Their natural hosts are various rodent species . ANDV is endemic in Argentina and Chile and its main reservoir host is the long-tailed pygmy rice rat (Oligoryzomys longicaudatus) . The virus causes hantavirus cardiopulmonary syndrome (HCPS) that is associated with a case fatality of up to 40%. Related HCPS-causing hantaviruses are found throughout the Americas. However, in contrast to other hantaviruses, ANDV can be transmitted from human to human [6, 7].
The hantavirus genome consists of three RNA segments: L (large), M (medium), and S (small) . The L segment encodes the 250-kDa L protein, which contains an RNA-dependent RNA polymerase (RdRp) domain in the center as indicated by sequence homology . The L protein is likely to possess additional enzymatic functions involved in transcription and replication of the RNA genome. The M segment contains the gene for the glycoprotein precursor, which is cleaved co-translationally into the envelope proteins Gn and Gc. The S segment encodes the nucleoprotein, which encapsidates the viral genome. Several hantaviruses encode a second gene product in the S segment, the non-structural protein NSs. This small protein seems to play a role in modulating the immune response of the host cell [10, 11].
The 5’ ends of hantavirus mRNAs contain a stretch of heterologous nucleotides suggesting that the virus uses a mechanism called ‘cap-snatching’ to initiate transcription of its mRNAs [12, 13]. Cap-snatching involves two steps. First, the 5’ end of a cellular mRNA is bound by a viral cap-binding protein and secondly, the cap is cleaved off several nucleotides downstream by a viral endonuclease. The capped RNA fragment is used as a primer to initiate transcription of the viral mRNA by the RdRp. The viral strategy of stealing the 5’ cap from host cell mRNA was first described for influenza A virus (IAV; family Orthomyxoviridae) . Here, the cap-binding and endonuclease domains reside in the PB2 and PA proteins, respectively [15–19]. In other segmented negative strand RNA viruses, the endonuclease resides in the very N terminus of the L protein, as shown for La Crosse virus (LACV; genus Orthobunyavirus, family Bunyaviridae), Lassa virus, and lymphocytic choriomeningitis virus (both family Arenaviridae) [20–23]. An endonuclease motif (H-PD-D/E-K) is also found in the N terminus of hantavirus L proteins, although there is no experimental evidence for a functional enzyme. The endonuclease is a potential target for antiviral therapy, as cap-snatching is an essential step in virus replication [24–35].
Investigation of L protein of ANDV and other hantaviruses has been challenging, because the protein is difficult to express in heterologous systems [36, 37]. We have previously shown that this phenotype is determined at post-transcriptional level and that mutations in the putative endonuclease can rescue L protein expression in mammalian cells . Both suggests that a strong catalytic activity of the ANDV endonuclease prevents high-level expression by down regulating the level of its own and host cell mRNAs.
The aim of the current study was to provide proof for the existence of a cap-snatching endonuclease in ANDV L protein using a structural and biochemical approach. We took advantage of the previously characterized mutations enhancing the L protein level in mammalian cells to facilitate expression of the endonuclease domain in bacteria.
For structural and biochemical characterization of the putative endonuclease of ANDV, we aimed to express the N terminus of L protein in E. coli. To this end, plasmids for expression of the N-terminal 163, 179, 191, 194, 197, 200, 211, 214, and 228 amino acid residues of wild-type ANDV L protein were generated using E. coli cloning strain DH5α. To facilitate purification, the expression constructs contained an N-terminal His-tag or His-tag plus maltose-binding protein (MBP), respectively. However, we encountered difficulties in expressing the L protein fragments in bacteria, as observed previously in mammalian cells [36, 37]. Proteins with less than 200 residues were found exclusively in inclusion bodies. Transformation of expression strains of E. coli with constructs containing 200 or more residues failed repeatedly, suggesting that background expression of soluble wild-type endonuclease is already toxic to the cells. To circumvent this problem, we took advantage of 15 amino acid exchanges that enhanced L protein expression in mammalian cells, presumably because they attenuate the enzymatic activity of the endonuclease . All 15 mutations were individually introduced into the plasmids for expression of L protein amino acid residues 1–200 fused to an N-terminal His-tag (ANDV L1–200) and the mutated constructs were tested for expression in E. coli BL21 cells. The results are summarized in Table 1. Two mutants (L1–200 Y32V and D37A) showed a phenotype like the wild-type protein, i.e. no bacterial growth was observed. The remaining 13 mutants (L1–200 R35H, H36R, D40E, I43A, K44A, N50A, P96A, D97E, N98A, E110A, K124A, K127A, and N167A) were successfully expressed with varying yield and purified via nickel affinity and size exclusion chromatography (S1 Fig). Noticeable slower growth of expressing E. coli was observed for L1–200 N167A. A thermofluor assay  was used to evaluate the stability of the mutant proteins under various conditions. The ANDV L1–200 mutants were most stable at high salt concentrations and low pH (S2 Fig), as expected from their calculated isoelectric point of approximately 7.5. Therefore, we used pH 5.5 and 1 M NaCl for protein purification. All mutants eluted from the size exclusion column according to their molecular mass, indicating they form monomers in solution.
ANDV L1–200 K127A was chosen for structural studies, as this mutant was the most stable mutant, which retained the capacity to bind to manganese ions—a co-factor of nucleases—along with a residual enzymatic activity (for details, see section on thermal stability below). The mutant was expressed as His-tag fusion of variable lengths (200, 211, 214, and 228 L protein residues) and subjected to crystallization trials. We succeeded to crystallize ANDV L1–200 K127A in the presence of Mn2+ after cleavage of the His-tag. The crystal structure was solved in space group P4212 by molecular replacement using coordinates for residues 32–162 from the endonuclease of Hantaan virus (, co-submission), allowing for the building of a contiguous polypeptide chain for one molecule in the asymmetric unit and refinement to a resolution of 2.4 Å (see S1 Table, 5HSB.pdb). Clear electron density was visible for the complete structure with the exception of the loop between the first two α-helices (residues 13–20), where the signal was weak. The structure clearly shows a Mn2+ ion bound to the active site (Fig 1A), as verified by an anomalous signal from data collected at 1.77 Å.
The crystal structure of ANDV L1–200 K127A and the comparison to the cap-snatching endonucleases of LACV, LCMV, and IAV [15, 19, 21–23] provides clear evidence for the N terminus of ANDV L protein being an endonuclease (Fig 1B). ANDV L1–200 has an overall structure similar to the other viral endonucleases with the highest similarity to its closest relative LACV. Considering the almost lacking sequence homology between the four proteins—except the catalytic H-PD-D/E-K motif—the structural homology is surprisingly high: RMSD values between the structural core (orange) of ANDV endonuclease and LACV, LCMV and IAV endonucleases are 1.7 Å, 1.9 Å and 2.2 Å, respectively (as given by DALI . The central β-sheet with the long α-helix αd that runs parallel to the β-sheet forms the core of the protein and provides the crucial active site residues in all four endonucleases (Fig 1A and 1B, shown in orange). Further structural elements are helical, although number, length, as well as position of the α-helices vary significantly between the structures. In all endonucleases, the active site is located in a groove between two lobes. One lobe is formed by a helix bundle that consists of two to three α-helices from the N terminus and at least one long α-helix (starting around residue 160) from the C-terminal end of the domain (Fig 1A and 1B, shown in green). The interaction between the last residues of the C-terminal helix and the first residues of the second helix stabilizes the 3-helix bundle (αa, αb, αf) and provides a plausible explanation why constructs with less than 200 residues were misfolded and thus insoluble. The other lobe contains less conserved structural elements consisting of two to three small helices (Fig 1A and 1B, shown in yellow). ANDV features a short additional α-helix αe between strand βc and the C-terminal helix αf, which is not present in LACV (Fig 1C).
One of the obvious differences between the endonuclease of ANDV and the other viruses are the overall dimensions of the domain: ANDV L1–200 is long and flat with approximate outer dimensions of 70 Å × 45 Å × 30 Å, whereas the other structures are more compact, in particular that of the distantly related IAV. The outer dimensions of the protein in the crystal fit with the dimensions in solution as determined by small angle X-ray scattering, indicating that the overall shape was not affected by crystallization artifacts (Figs (Figs1D1D and S3).
Most of the surface of ANDV L1–200 is formed by the helix bundle composed of the N- and C-terminal helices αa+b and αe+f, and the three small helices αc, αc’ and αc” (Fig 1A and 1B). These helices differ between the endonucleases not only in their conformation and orientation, but also in their amino acid composition. Fig 2A shows how this leads to a significant alteration in surface charge distribution compared to the other enzymes. The ANDV endonuclease has large positively charged patches surrounding the active site groove, which are not or only to a lesser extent present in LACV and LCMV or even replaced by negatively charged areas. The more positively charged surface may increase the binding affinity of the enzyme to its substrate, i.e. negatively charged mRNA.
A closer look at the active site of the ANDV endonuclease (Fig 2C) in comparison to LACV and IAV demonstrates the structural similarity of the essential catalytic residues. The side chains of the conserved H-PD-D/E-K motif superimpose well (Fig 2B). The manganese bound to the active site also superimposes with one of the two metal ions present in the other structures. Sequence comparisons suggested that the active site of the ANDV endonuclease is more closely related to IAV than LACV [20, 37]. Indeed, Lys124 of ANDV superimposes with Lys134 of IAV, whereas LACV has a threonine at this position and the equivalent lysine (Lys94) is provided by a different part of the protein.
Close to the active site, electron density for a sulfate and a glycerol molecule is visible in the structure (Fig 2D). The glycerol ligand is coordinated by the conserved residues Tyr32 and Arg35 (Fig 2E), both of which have previously been proposed to regulate the endonuclease activity of the L protein .
With the crystal structure of the ANDV cap-snatching endonuclease at hand, we may infer a more precise role for the 15 amino acid residues that have been implicated in the activity of the endonuclease in mammalian cells  (Fig 3). The residues can be allocated to five different groups according to their presumed role: (1) catalytic residues (His36, Asp97, and Glu110), (2) active site residues not essential for activity (Asp37, Pro96, Lys124, and Lys127), (3) residues involved in RNA binding in the vicinity of the active site (Tyr32 and Arg35), (4) residues forming stabilizing contacts close to the active site (Asp40, Ile43, Lys44, Asn50, and Asn98), and (5) residues stabilizing helix αe (Asn167). The hypothetical role also considers biochemical and thermal stability data (Table 1 and sections below).
His36, Asp97, and Glu110 (group 1) are the central catalytic residues coordinating the manganese ion (Figs (Figs2C2C and and3).3). Asp37 and Pro96 (group 2) are relevant for positioning the catalytic residues His36 and Asp97, respectively. Lys124 and Lys127 (group 2), which emerge from helix αd, may position the RNA substrate in the active site. Tyr32 and Arg35 (group 3) are located in helix αb and bind a glycerol solvent molecule in the structure. Their position at the entrance of the catalytic site and capacity to bind hydroxyl groups let us to speculate that these residues may also be involved in binding the negatively charged RNA substrate. Asp40, Lys44, Asn50, and Asn98 (group 4) form hydrogen bonds with each other and main chain atoms to stabilize and position the loop that contains the catalytic Asp97. Ile43 forms hydrophobic interactions between the end of helix αb and several hydrophobic side chains both from the β-sheet and from helix αe. Group 5 contains only Asn167. It is located in the small helix αe, which is not present in the LACV endonuclease (Fig 1D) and stabilizes this helix via hydrogen bonds with main chain atoms of strand βc.
The thermal stability of the purified proteins without and with manganese was measured by thermofluor assay  in analogy to previously described experiments with the IAV and LACV cap-snatching endonucleases [16, 20]. Melting temperature (Tm) as a measure of stability varies from 34.2°C for the least stable L1–200 I43A mutant to 49.2°C for the most stable L1–200 K124A mutant (Fig 4A and 4B). For most mutants, the Tm increased by approximately 7°C in the presence of 4 mM manganese, presumably due to binding of the metal in the catalytic site. Consistent with this explanation, mutation of catalytic residues directly involved in metal coordination (His36, Asp97, and Glu110) abolishes this stabilizing effect. Interestingly, increasing the manganese concentration to 16 mM led to a substantial additional Tm increase of up to 8°C for some mutants, suggesting binding of another metal ion by the active site (Fig 4A and 4B). In contrast to manganese, magnesium ions showed a much weaker effect (Fig 4A and 4B).
Enzymatic activity was measured in a ribonuclease assay. A 5’-radioactively labeled 27mer single stranded (ss) RNA molecule was used as substrate, and the level of activity was measured by substrate degradation. None of the mutants was active in the absence of divalent ions. Addition of manganese rescued activity for some mutants (Fig 5A), while magnesium had no enhancing effect at all. Therefore, all further assays were performed in the presence of manganese. As expected, active site mutants L1–200 H36R, D97E, and E110A were inactive despite addition of manganese (Fig 5A and 5B). Mutation of Arg35, Lys124, and Lys127, still allowed for very low residual activity. Mutation of Asn50 and Pro96 stabilizing the active site loop also strongly reduced endonuclease activity. Mutations affecting stability of the tertiary structure (D40A, I43A, K44A, N98A, and N167A) allowed for intermediate to high activity. We could not detect an intermediate cleavage product of specific length (S4 Fig). The most active mutant was L1–200 N167A, which was therefore chosen for further biochemical experimentation. Thermal stability and enzymatic activity data are summarized in Table 1.
To investigate substrate specificity of the ANDV endonuclease, we used three mutants with a range of enzymatic activity: L1–200 N167A with high activity, L1–200 K44A with intermediate activity, and L1–200 D97E as a negative control. First, four different substrates were tested: a 27mer ssRNA that can form secondary structures (predicted hairpin Tm = 77°C), the same sequence as double stranded (ds) RNA, and the corresponding ssDNA and dsDNA molecules (Fig 5C). ANDV L1–200 N167A and K44A were most active on ssRNA, though they can also less efficiently cleave dsRNA. None of the mutants was active on ssDNA or dsDNA, indicating that the enzyme is a ribonuclease. Furthermore, we compared the ribonuclease activity on ssRNA with different sequence, structure, and length (Fig 5D). Comparison of the 27mer RNA with hairpin-like structure vs. a 27mer unstructured poly(A) RNA reveals preference for the unstructured substrate. In addition, longer RNAs are degraded faster than shorter RNAs. The negative control (L1–200 D97E) did not show background degradation, demonstrating that our purification procedure removes potentially contaminating bacterial nucleases.
The metal-dependent cap-snatching endonuclease is an attractive target for antiviral drugs against segmented negative strand RNA viruses and promising compounds have been described for influenza [24–35]. To proof suitability of the ANDV endonuclease expressed in this study for screening of potential inhibitors, we first measured the effect of the classical influenza virus endonuclease inhibitor 2,4-dioxo-4-phenylbutanoic acid (DPBA)  on thermal stability of ANDV L1–200 mutants H36R, K44A, N50A, D97E, E110A, K125A, K127A, and N167A in the presence of 16 mM MnCl2. All mutants that showed a stabilization of > 8°C in the presence of manganese, also showed a further increase in the melting temperature upon addition of DPBA (Fig 6A), indicating that DPBA binds to and stabilizes the manganese–active site complex. In agreement with this conclusion, mutation of residues His36, Asp97 and Glu110, which are involved in coordination of at least one manganese ion, abolishes the stabilizing effect of DPBA.
To prove that DPBA not just binds to the active site of ANDV L1–200, but also inhibits the ribonuclease activity, we incubated the most active mutant ANDV L1–200 N167A with Mn2+ and DPBA for 15 min prior to the addition of the RNA substrate. Fig 6B and 6C show a clear dose-dependent inhibition of the enzymatic activity comparable to IAV and LACV [16, 20].
This study provides structural and biochemical evidence for the existence of an endonuclease in the N terminus of ANDV L protein. In agreement with previous studies on expression of L protein in mammalian cells [36, 37], we have not been able to express the wild-type endonuclease domain in bacterial cells. Therefore, we took advantage of a range of mutations to facilitate expression of the domain. By using this approach, we expressed large amounts of various mutants and eventually solved the crystal structure of the ANDV L1–200 K127A mutant. The first structure of a hantavirus cap-snatching endonuclease reveals similarities to the related enzymes from LACV, LCMV, and IAV [16, 18, 20–23]. Besides the active site residues the sequence is hardly conserved. However there are obvious common structural features present: all cap-snatching endonucleases consist of two lobes with the conserved active site buried in a cavity in between. However, compared to the other enzymes, ANDV has positively charged patches surrounding the active site. The positively charged surface may increase the affinity of the enzyme for negatively charged RNA substrates. Whether or not this feature plays a role in the apparently strong endonuclease activity upon recombinant expression remains to be determined.
The structure in conjunction with the thermal stability and enzymatic activity data of the attenuated mutants allow us to speculate on the contribution of individual residues for enzymatic activity, substrate binding, and overall stability of the tertiary structure (Table 1). The data demonstrate that residues His36, Asp97, and Glu110 coordinate the manganese ion and are essential for the catalytic process. All three residues act in a cooperative manner, as removal of one residue is sufficient to prevent binding of both metal ions and inactivate the enzyme. The two lysine residues at positions 124 and 127 in the active site may stabilize the attacking hydroxide nucleophile during catalysis and/or bind to the RNA substrate, as speculated previously for IAV and LACV [16, 20]. Mutation of both lysines does not affect the stabilizing effect of DPBA, suggesting they play no essential role in metal binding. The two residues Tyr32 and Arg35, which coordinate a glycerol ligand in the crystal structure, may also be involved in substrate binding. Arg35 seems to be of particular relevance, as it is conserved in all bunyavirus L proteins and the L1–200 R35H mutant is largely defective, although it clearly binds manganese in the thermal stability experiments. Tyr32 seems to be less important, as the L1–200 Y32V mutant behaves like the wild-type protein and is toxic to E. coli cells.
Interestingly, several attenuating mutations (D40E, I43A, K44A, N50A, N98A, and N167A) seem to slightly destabilize the protein, which might affect the enzymatic activity of the enzyme. The corresponding wild-type residues are involved in hydrogen bonds with other side or main chain atoms or in hydrophobic interactions with neighboring secondary structure elements. Consistent with the structural data, most of these mutants are characterized by low temperature stability and high residual enzymatic activity. Residue Asn167 is exceptional as it is located distant to the active site. Via hydrogen bonds with β-sheet βc it positions a small helix (αe) that is not present in the otherwise similar LACV protein. Consistent with the peripheral location of Asn167, the corresponding mutant is the least attenuated. Its wild-type like features include the highest enzymatic activity and a reduced growth rate of the expressing E. coli cells. Given the strong activity of an attenuated version of the enzyme on various RNA templates, it is plausible that the wild-type enzyme activity is too toxic for high-level expression in pro- and eukaryotic cells. Hantaviruses must have an intricate mechanism to control the activity of the endonuclease in infected cells or to keep the concentration of the L protein at a sub-toxic level.
The biochemical data also revealed a clear co-factor and substrate specificity of the ANDV endonuclease. Consistent with its presumed function as a cap-snatching enzyme, it preferred ssRNA over dsRNA substrates and was inactive on ssDNA or dsDNA. RNA length and secondary structure seem to influence substrate binding. Dependence of enzymatic activity from manganese rather than magnesium has also been observed for the endonucleases of the other segmented negative strand viruses [16, 20, 21]. However, in contrast to LACV and IAV with two metal ions in the active site [16, 20], the ANDV crystal structure contains only a single manganese ion (equivalent to Mn1) , while the LCMV structure does not contain any ions in the active site. The reason for this discrepancy may be explained by our crystallization conditions and thermal stability data. At 2 mM manganese, the active site in the crystals contained a single ion. However, we observed an additional increase in thermal stability when increasing the manganese concentration from 4 mM to 16 mM, suggesting the binding of an additional ion (equivalent to Mn2) at higher manganese concentration. This is in agreement with findings by Reguera et al. 2016  for related endonucleases showing a high binding affinity for the first manganese ion (Mn1), but low affinity for the second manganese ion (Mn2). It is reasonable to propose that ANDV and other hantaviruses share the same two-metal dependent catalytic mechanism with LACV and IAV .
This study also has implications for development of antiviral drugs to treat hantavirus infections. The influenza virus endonuclease has been used as a target in antiviral drug discovery programs and several specific inhibitors have been found [24–35]. Likewise, the ANDV endonuclease is an attractive target for antiviral drug development. The experiments with the known endonuclease inhibitor DPBA  provide proof-of-concept that the ribonuclease activity of the enzyme is amenable to compound screening. The highly active ANDV L1–200 N167A mutant is the most promising enzyme candidate for this purpose.
The cDNA of fragments encoding residues 1–163, 1–179, 1–191, 1–194, 1–197, 1–200, 1–211, 1–214 and 1–228 from the L protein of ANDV (strain Chile 9717869, GenBank accession no. AF291704) were amplified by PCR using the pCITE-ANDV-L plasmid  as a template. Virus sequences were cloned into pOPIN-F (N-terminal His-tag–3C protease cleavage site) and pOPIN-M (N-terminal His-tag–maltose binding protein–3C protease cleavage site) . L protein mutants (Y32V, R35H, H36R, D37A, D40E, I43A, K44A, N50A, P96A, D97E, N98A, E110A, K124A, K127A and N167A) were generated via a classical two-step PCR mutagenesis approach as described previously  and the resulting fragments cloned into pOPIN-F. For cloning, E. coli strain DH5α was used. The sequence of the inserts was confirmed by sequencing.
Proteins were expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17°C overnight using LB medium and 0.5 mM isopropyl-β-D-thiogalactopyranosid for induction. Cells were pelleted, resuspended in lysis buffer containing 50 mM Tris-HCl pH 7.3, 300 mM NaCl, 10 mM imidazole, 10% glycerol, 10 mM MnCl2, and 1 mM phenylmethylsulfonyl fluorid and disrupted by sonication. The soluble material was loaded onto a Ni-NTA affinity column, washed with 10 column volumes of lysis buffer containing 50 mM imidazole, and eluted with 5 volumes of lysis buffer containing 500 mM imidazole. The His-tag of eluted protein was cleaved off using glutathione S-transferase-tagged 3C protease at 4°C overnight during dialysis against lysis buffer. The untagged proteins were further purified by size exclusion chromatography using a Superdex 200 column in 50 mM sodium citrate pH 5.5, 1 M NaCl, 5% glycerol. Purified proteins were concentrated using centrifugal devices, flash frozen in liquid nitrogen, and stored in aliquots at –20°C.
ANDV L1–200 K127A (10 mg/ml) was crystallized in the presence of 2 mM MnCl2, 100 mM sodium acetate pH 5.0, 27.5% polyethylene glycol 3350, and 500 mM (NH4)2SO4 by sitting drop vapor diffusion. Crystals were flash frozen in liquid nitrogen with 8% butanediol as cryo protectant and diffraction data were collected to 2.4 Å at beamline ID23-1 at the European Synchrotron Radiation Facility, Grenoble, with a wavelength of 0.98 Å and 1.77 Å for respectively native and anomalous data. Datasets were processed with iMosflm . The crystal structure was solved in space group P4212 by molecular replacement using residues 32–162 from the endonuclease from Hantaan virus  and PHASER , and refined by iterative cycles of manual model building in Coot  and computational optimization with PHENIX . TLS refinement was performed during the final stages of refinement  with the entire molecule as rigid group. Data collection and refinement statistics are shown in the crystallographic table in the supplemental information. Structural data were visualized with Pymol and Chimera . Electrostatic surfaces were calculated using PDB2PQR and APBS [49, 50]. The coordinates have been deposited to the PDB (5HSB.pdb).
Small angle X-ray scattering (SAXS) data for 1, 2, and 5 mg/ml of ANDV L1–200 K127A in 2 mM MnCl2, 50 mM Tris-HCl pH 7.3, 250 mM NaCl, 5% glycerol were collected at the SAXS beamline P12 at the PETRA III storage ring of the Deutsches Elektronen-Synchrotron, Hamburg . Using a PILATUS 2M pixel detector at 3.1 m sample distance and 10 keV energy (λ = 1.24 Å), a momentum transfer range of 0.01 Å–1 < s < 0.45 Å–1 was covered (s = 4π sinθ/λ, where 2θ is the scattering angle). Data were analyzed using the ATSAS 2.6 package . The forward scattering I(0) and the radius of gyration Rg were extracted from the Guinier approximation calculated with the AutoRG function within PRIMUS . GNOM  provided the pair distribution function P(r) of the particle and the maximum size Dmax. Ab initio reconstructions were generated with the program DAMMIF . Ten independent DAMMIF runs were superimposed by SUPCOMB  and averaged using the program DAMAVER .
Nuclease activity was measured by incubating 1 μM ANDV L1–200 protein with 0.1 μM 32P-labeled single stranded (ss) or double stranded (ds) 27mer RNA or DNA substrate (5’-GAU/TGAU/TGCU/TAU/TCACCGCGCU/TCGU/TCGU/TC-3’) or 40mer ssRNA substrate (5´-GAUGAUGCUAUCACCGCGCUCGUCGUCGAUGAUGCUAUCA-3’) in 50 mM Tris-HCl pH 7.3, 250 mM NaCl, 5% glycerol, 0.25 U/μl RNasin (Promega) in the absence or presence of 2 mM MnCl2 at 37°C for 1 or 2 h. RNA was annealed by heating to 98°C for 2 minutes, followed by a slow cooling over 2 hours to room temperature. A 27 or 40mer poly(A) ssRNA was used as unstructured substrate. In selected experiments, 2,4-dioxo-4-phenylbutanoic acid (DPBA) was added to the assay. The reaction was stopped by adding 2 × loading buffer (95% formamide, 18 mM ethylenediaminetetraacetic acid (EDTA), 0.025% sodium dodecyl sulfate, xylene cyanol, and bromophenol blue) and heating the samples to 98°C for 5 min. The reaction products were separated by 8 M urea, 20% polyacrylamide, Tris-borate-EDTA gel electrophoresis and visualized by phosphor screen autoradiography using a Typhoon scanner (GE Healthcare). Intensity of the signals was quantified by ImageJ software .
The stability of ANDV L1–200 protein was measured by thermofluor assay at a protein concentration of 10 μM in 100 mM Tris pH 7.0, 250 mM NaCl, 5% glycerol complemented with either 10 mM EDTA, 4 mM MnCl2, 16 mM MnCl2, 16 mM MgCl2 or 16 mM MnCl2 plus 100 μM DPBA as described .
Following purification, 1 μg of protein was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and stained with Coomassie. The His-tag of ANDV L1–200 K127A had been cleaved off for crystallization experiments. All other proteins feature an N-terminal His-tag.
The stability of the purified protein was tested in thermofluor assay under various conditions. The protein is most stable at lower pH and higher salt concentrations.
Experimental scattering curves (black dots) are compared with theoretical scattering curves for the ANDV crystal structure (black line), the LACV crystal structure (blue line), the LCMV crystal structure (green line) and the IAV crystal structure (orange line). χ2 –values of fits are given for each structure, curves were calculated with CRYSOL .
Degradation of unstructured polyA RNA by ANDV L1-200 N167A shows no accumulation of larger intermediate RNA products with specific lengths.
We thank Sven Falke and Markus Perbandt for help at X-ray sources and the beamline staff at P12 (PETRA III) and ID23-1 (ESRF) for their support. We thank Maria Rosenthal and Patrick Heinemann for discussions.
YFG received funding from Comisión Nacional de Investigación Científica y Tecnológica, CONICYT (21110444 and BCH2014-7571) www.conicyt.cl. This work was also funded by grants from the Deutsche Forschungsgemeinschaft (DFG): RE 3712/1-1 to CB and SR and GRK1721 to GW, www.dfg.de/en. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
The crystal structure is available from the pdb (accession number 5HSB.pdb).