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Medicine (Baltimore). 2016 May; 95(19): e3640.
Published online 2016 May 13. doi:  10.1097/MD.0000000000003640
PMCID: PMC4902523

Effectiveness of Essence of Chicken in Improving Cognitive Function in Young People Under Work-Related Stress

A Randomized Double-Blind Trial
Monitoring Editor: Gokhan Cuce.


Work-related stress (WS) can result in considerable and extensive changes in physiological and psychological performance. WS beyond the optimal levels induces anxiety, confusion, exhaustion, and burnout. Chronic WS affects neurocognitive performance, particularly attention and visuospatial memory. Essence of chicken (EC) has been reported to improve neurocognitive function after mental stress.

To investigate the beneficial effects of EC in improving neurocognitive performance under WS, we conducted a randomized, double blind trial. Total 102 young workers in New Taipei City with high WS, evaluated using the Individual Subjective Perception Job Stress Scale scores (>36 for job leaders and 33 for nonleaders) were recruited. Fifty-one participants received 70 mL of EC and 51 received a placebo daily for 2 weeks. Blood tests and neurocognitive assessment were performed before treatment, at the end of treatment, and 2 weeks after treatment.

EC improved the performance of participants with high depression scores in the form-color associative memory test, used for assessing short-term memory. Although creatinine and glutamic-pyruvic transaminase (GPT) levels increased in week 2, but the levels returned to the baseline in week 4. Blood urea nitrogen (BUN) levels decreased in week 4.

EC significantly improved short-term memory in participants with high WS and concomitant depressive mood, although it slightly increased GPT and creatinine levels and reduced BUN levels. The long-term treatment effects of EC warrant further investigation.


The perception of a stimulus as overwhelming causes stress, which in turn elicits a measurable response resulting in a transformed state.1 Stress is usually caused by pressure or demand that affects a person's coping ability. Work-related stress (WS) usually occurs because of imbalance between the demands of a job and the resources and capabilities of the worker to meet these demands.2 WS can result in significant and extensive changes in physiological and psychological performance. Although stress can improve job efficiency, WS beyond the optimal levels induces anxiety, confusion, exhaustion, and burnout.36 Chronic WS affects neurocognitive performance, particularly attention and visuospatial memory, in which the frontal–medial temporal cortex network is involved. A previous study proposed decline in neurocognitive functions to be associated with low quality of life and a reduced adrenocorticotropic hormone (ACTH) response to corticotrophin-releasing hormone.7 In addition, chronic WS reduced brain volumes of the anterior cingulate cortex, dorsolateral prefrontal cortex, caudate, and putamen.8 Few reports have documented that stress might alter the immunity status. Levels of monocyte-produced antiinflammatory cytokine IL-10 increased in burnout syndrome, a severe consequence of WS.9,10 In a study on nurses working in an emergency department, the secretion rates of salivary IgA and lysozyme significantly decreased with an increase in WS levels.11

Essence of chicken (EC) is a liquid nutritional supplement prepared by cooking whole chickens for several hours at a high temperature. In traditional Chinese medicine, EC is used for strengthening the musculoskeletal system, spleen, and stomach and for enhancing vigor. EC contains minerals, vitamins, trace elements, indispensable amino acids, and nutrients such as carnosine and anserine.12,13 EC has been extensively studied and is known to affect multiple organ systems (immune,14 cardiovascular, and renal systems15) and physiological processes (erythropoiesis16 and circadian rhythm, specifically jet lag17) and reverses chemotherapy-induced bone marrow suppression.18 In previous reports, EC exerted antistress and antifatigue effects by regulating the cortisol levels and activating the brain histaminergic system.1921 In stressed animals, EC alleviated stress-induced fatigue by preventing stress-mediated dysfunction caused by elevated insulin levels and increasing lipase activity and glycogen synthesis.22 Many clinical studies have showed the beneficial effects of EC on maintaining and enhancing neurocognitive performance, particularly attention and short-term memory under stressful conditions.20,23 The antistress effects of EC were attributed to enhancement of cortisol levels.20 On the basis of previous research, we hypothesize that EC can serve as a neurocognitive enhancer and antistress food, promote resilience and resistance to stress, and maintain neurocognitive functions. However, well-designed clinical trials, particularly randomized, double blind trials, for testing the effects of EC are lacking. Therefore, the aim of the current study was to investigate the beneficial effects of EC in improving neurocognitive performance under WS in a randomized, double blind trial.


Study Design

This study had a randomized, double blind trial design and was approved by the Joint Institutional Review Board of Taipei Medical University. The study was registered with the registration number NCT02166931. The participants and investigators were both blinded to the participant groups. Participants who met the inclusion criteria were randomly administered BRAND essence of chicken (BEC) or a placebo daily (70 mL) for 2 weeks after enrollment (Figure (Figure1).1). Neurocognitive assessment and blood tests were performed at week 0 (before treatment), week 2 (at the end of treatment), and week 4 (2 weeks after treatment).

Summary of the randomized, double blind trial.

Participants and WS Evaluation

All potential participants were evaluated using Individual Subjective Perception Job Stress Scale (ISPJSS) scores before randomization. The ISPJSS is a 20-item self-report questionnaire adapted from the Occupational Stress Scale created by the Institute of Occupational Safety and Health of Taiwan. The Cronbach alpha of this questionnaire is 0.96, and the coefficient of internal consistency is 0.87. The participants answered all items on a 4-point fully labeled Likert scale (0 = not much; 3 = very much), and their responses were summed to obtain a total score.24 The inclusion criteria were: an age of 20 to 65 years with at least 9 years of education and provision of written informed consent, no chronic systemic diseases treated using medication, a score >36 for job leaders and 33 for nonleaders on the ISPJSS,24 and no current use of medication that affects the mental state or neurocognitive functions. The exclusion criteria were: existing or previous psychiatric disease (bipolar disorder and schizophrenia), neurological disease (alcoholic encephalopathy and epilepsy), cerebrovascular disease, Parkinson disease, or other neurodegenerative disease, the use of any medication, such as antianxiety and sedative drugs, that would affect the mental state during the trial, and a history of allergy to chicken. For understanding the emotional status of the participants at the baseline, the Beck depression inventory (BDI) and Beck anxiety inventory (BAI) were implemented.

EC and Placebo

BEC and the placebo had a similar appearance and taste. BEC (70 mL), provided by Cerebos Pacific Ltd, Singapore, contains 83 mg of protein and peptide, 3.1 mg of free amino acids, 0.8 mg of hexose, 0.4 mg of fat, and 3 mg of caramel.25 It also contains the active dipeptides carnosine (b-alanyl-l-histidine) and anserine (b-alanyl-l-methyl-l-histidine).15,22 The placebo (70 mL) contained 83 mg of milk casein and 3 mg of caramel and thus had a protein content, caloric content, and color similar to those of the BEC.

Neurocognitive Function Assessment

Clinical neuropsychologists blinded to the participant groups performed neurological assessments. The neurocognitive functions of the participants were assessed using a neuropsychological test battery comprising the form-color associative memory test, continuous performance test, word list learning test, digit cancellation test, digit span subtest of the Wechsler Memory Scale (WMS-III), letter number sequencing subtest of the WMS-III, paced auditory serial addition test, and digit vigilance test. Neurocognitive functions such as sustained attention, vigilance, working memory, executive functions (mental flexibility and control, attention division and disinhibition), and short-term memory (registration, delayed recall, and retention) were assessed (Table (Table11).

Neurocognitive Functions Surveyed in This Study and the Respective Tests

Blood Tests

Fasting glucose, renal function (blood urea nitrogen [BUN], creatinine), liver function (glutamate oxaloacetate transaminase, glutamic-pyruvic transaminase [GPT]), ACTH, cortisol, and melatonin levels, and the ratio of classification determinant 4+ cells/classification determinant 8+ cells (CD4+/CD8+) were measured in the peripheral blood of the participants at approximately 8:00 am at each visit.

Statistical Analysis

Demographic characteristics and baseline biochemistry profiles were compared between the BEC and control groups, by using the Student t test for continuous variables and the Chi-square test for categorical variables. Generalized estimation equation models were used for comparing neurocognitive function and biochemistry profiles between the groups. To account for multiple comparisons, we calculated the false discovery rate using the Benjamini–Hochberg procedure.26 All statistical analyses were performed using SAS Version 9.4 (SAS Institute Inc., Cary, NC) considering 2-sided probabilities.


The demographic information of the participants is listed in Table Table2.2. All 102 participants, 51 in each group, completed the trial and no adverse events were noted. No differences in gender, education, and ISPJSS, BDI, and BAI scores were observed between the groups. The mean age of the participants in the BEC group (34.1 ± 4.4 years, mean ± standard deviation) was significantly higher than that in the control group (32.2 ± 4.2 years). There are no differences between groups in all the blood tests except melatonin in the baseline. The mean melatonin concentration of BEC group, 16.9 ± 12.9 pg/mL, is higher than that of control group, 12.6 ± 6.1 pg/mL, P < 0.05. Table Table33 lists the outcomes of neurocognitive function assessments in the 2 groups. No significant changes in the neurocognitive test results were observed in the 2 groups after multiple testing. Table Table44 illustrates changes in biochemical values in the BEC and control groups. No differences were observed in the blood tests after treatment compared with those at the baseline or between the 2 groups.

Demographics and Baseline Anxiety, Depression Profiles of the Participants
Neurocognitive Function Survey of the Participants
Biochemistry Profiles at Weeks 0, 2, and 4

To understand the effects of BEC on the neurocognitive functions in participants with high mood disturbance, we analyzed participants with high anxiety scores in the BAI (total score > 25) or high depression scores in the BDI (score > 28). Among the participants with high anxiety scores, 9 received BEC and 14 received the placebo. In the BEC group, the mean age was 34.1 ± 5.0 years and the female/male ratio was 7/2; in the control group, the mean age was 31.7 ± 4.8 years and the female/male ratio was 9/5. The baseline values of all form-color associative memory tests were similar between BEC and control groups. Figure Figure22 reveals that performance in trial 1 of the form-color associative memory test borderline significantly improved in the BEC group among the participants with high anxiety scores after multiple test correction (P = 0.0954).

Mean changes in the form-color associative memory test from week 0 to week 4 in participants with high anxiety scores.

Among the participants with high depression scores, 11 received BEC and 21 received the placebo. In the BEC group, the mean age was 35.2 ± 4.9 years and the female/male ratio was 7/4; in the control group, the mean age was 32.0 ± 4.0 years and the female/male was 13/8. The baseline values of all form-color associative tests were about the same between these 2 groups. The BEC group exhibited significantly superior performance in trial 1 (P < 0.018), and total score of the form-color associative memory test than the control group after multiple testing (P < 0.018) (Figure (Figure33).

Mean change in the form-color associative memory test from week 0 to week 4 in participants with high depression scores.


WS affects neurocognitive functions, but effective treatments for promoting resilience and resistance to stress-associated effects and maintaining neurocognitive functions are lacking. EC has been widely studied and empirical data suggest that EC is beneficial to multiple organ systems and can improve attention and short-term memory under extremely stressful conditions. In this randomized double-blind trial, we recruited participants with high WS according to ISPJSS scores (>36 for job leaders and 33 for nonleaders). Comprehensive neurocognitive function assessment, blood biochemistry examination, and immunity surveys were implemented at the baseline (week 0), after EC treatment for 2 weeks (week 2), and 2 weeks after the treatment (week 4). To the best of our knowledge, the present study is the 1st randomized double-blind trial for assessing the clinical application of EC.

Multiple neurocognitive function domains, such as attention or vigilance, working memory, immediate and delayed recall of memory, learning effects, retention of memory, and executive functions, were examined in this trial. Almost all neurocognitive functions improved in week 2 and week 4 in both groups. This improvement could be attributed to learning effects attributable to short intervals between the tests (Table (Table3).3). However, participants in the BEC group with high depression scores exhibited greater improvement in trials 1 and 2 of the form-color associative test and total scores than those in the control group did. In addition, participants in the BEC group with high anxiety scores exhibited greater improvement in trial 1 of the form-color associative test than those in the control group did. Therefore, we conclude that BEC did not induce significant improvement of neurocognitive functions when we pooled all participants; however, BEC improved the short-term memory of the participants with high depression scores and slightly improved the short-term memory of the participants with high anxiety scores. The form-color memory associative memory test depends on sound short-term memory functions. Stable concentration, working memory, integrated ability, mental control, and an applicable strategy are other factors evaluated in this test. In trial, the participants had to learn extensive and unrelated information immediately and hence may have felt extremely nervous and anxious, which are called the 1st-trial effects. An anxious participant may face difficulty in managing the 1st-trial effects, resulting in monitor errors. Therefore, it is speculated that BEC might be beneficial for controlling inner anxiety under highly stressful conditions in people with high anxiety scores.

In previous studies, BEC improved cognitive function and attention in a short-term mental fatigue scenario.20,27 Another study reported that BEC improved cognitive functions such as attention and memory in medical students.23 Through near-infrared spectroscopy, BEC was determined to increase oxyhemoglobin concentrations in several regions of the prefrontal areas of the brain, and this area appears to play a fundamental role in short-term memory and working memory. These changes might contribute to the improvement of neurocognitive function.28 However, our study was a short-term study, and the effects of long-term BEC use and the mechanisms through which BEC induces neurocognitive improvement remain unknown. Neurocognitive function improved in participants with WS and concomitant depression in this trial. Thus, whether BEC-induced improvement in neurocognitive function increases work efficiency and reduces errors at the workplace in highly stressed workers warrants further investigation.

BEC did not alter the blood biochemistry profile, including liver function; ACTH, cortisol, melatonin, and blood sugar levels; and immunity (examined according to the CD4+/CD8+ ratio). BEC significantly increased creatinine levels from 0.68 to 0.73 mg/dL, and they returned to 0.70 mg/dL after discontinuation of BEC use. BUN levels decreased in week 4 compared with the baseline data. In an animal study, EC reduced creatinine and BUN levels.15 Increased creatinine levels may be caused by various factors. EC contains arginine and glycine, which increase creatine production in the muscle cells, and creatine can be metabolized to creatinine. Blood GPT levels slightly decreased in week 4. The effects of BEC on increasing GPT, creatinine, and BUN levels have not been reported earlier. As GPT, creatinine, and BUN levels were within normal ranges, the clinical significance and long-term effects of BEC on GPT, BUN, and creatinine remain uncertain.

There are a few limitations in this study. First, the intervals between the neurocognitive tests were short. Learning effects may occur and might interfere with the real effects of BEC, although BEC demonstrated its benefits on neurocognitive improvement in this study, particularly for short-term memory. Second, the precise effects of WS on neurocognitive function were not measured. All participants were recruited according to WS scores; however, the influence of WS on neurocognitive functions might be variable. The number of participants in this trial was relatively high; therefore, individual variation might be neglected. Third, the BEC treatment in this trial was short, and the long-term effects of BEC warrant further investigation. Imbalance was observed in the number of participants with high mood disturbance in the BEC and control groups. This study was a double blind randomized trial; therefore, the results were neither unpredictable nor preventable.

In summary, this randomized double-blind trial of young workers under high work stress revealed that BEC treatment for 2 weeks significantly improved short-term memory in the participants with high depression scores and slightly increased GPT and creatinine levels and reduced BUN levels.


The authors thank Cerebos Pacific Ltd (Singapore) for providing BRAND'S essence of chicken, the placebo, and financial support for this trial. The authors also maintain complete control over the direction and content of the manuscript, Clinical trial registration number: NCT02166931 (


Abbreviations: ACTH = adrenocorticotropic hormone, BAI = Beck anxiety inventory, BDI = Beck depression inventory, BEC = BRAND essence of chicken, BUN = blood urea nitrogen, EC = essence of chicken, GPT = glutamic-pyruvic transaminase, ISPJSS = Individual Subjective Perception Job Stress Scale, PASAT = paced auditory serial addition test, WS = work-related stress.

Y-CH and C-JH contributed equally and H-MW and K-YC contributed equally to this work.

The authors have no conflicts of interest to disclose.


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