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J Vet Intern Med. 2015 Nov-Dec; 29(6): 1502–1509.
Published online 2015 September 29. doi:  10.1111/jvim.13634
PMCID: PMC4895649

Pulmonary Vein‐to‐Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease

Abstract

Background

Early recognition of left‐sided congestive heart failure (CHF) in dogs with degenerative mitral valve disease (DMVD) is important because it influences medical therapy, timing of follow‐up, and outcome.

Hypothesis

Pulmonary vein diameter‐to‐pulmonary artery diameter ratio (PV/PA) measured by echocardiography can predict CHF.

Animals

Ninety‐eight client‐owned dogs, 37 controls, and 61 dogs with DMVD.

Methods

Prospective clinical cohort study. History, physical examination and Doppler‐echocardiography were performed. Dogs were classified as International Small Animal Cardiac Health Council class I, II or III. Congestive heart failure was identified in a subset of 56 dogs based on radiographic findings. The PV/PA was measured in bidimensional (2D) and M‐mode by 2 investigators blinded to the radiologists’ conclusions.

Results

Interobserver coefficients of variation for PV/PA acquisition and measurement were <10%. The PV/PA in control dogs was approximately 1 and increased with class of heart failure. The presence of CHF could be best predicted by measuring PV/PA in 2D echocardiography (cut‐off, 1.7; area under the curve, 0.98; CI, 0.97–0.98; P < .001) with a sensitivity of 96% and a specificity of 91%.

Conclusion and clinical importance

The PV/PA is a simple and reproducible echocardiographic variable that increases with class of heart failure and may help discriminate dogs in CHF from asymptomatic dogs with DMVD. Additional studies are required to determine whether PV/PA might provide additional information in the integrated interpretation of Doppler‐echocardiographic indices of left ventricular filling pressures and could be used for rapid assessment of CHF in dogs in a critical care setting.

Keywords: Canine, Congestion, Diagnosis, Pulmonary vein

Abbreviations

2D
bidimensional
ACEI
angiotensin converting enzyme inhibitor
AUC
area under the curve
Ao
aorta
BW
body weight
CHF
congestive heart failure
CI
confidence interval
CV
coefficient of variation
DMVD
degenerative mitral valve disease
E/A
maximal velocity of early left ventricular diastolic filling/maximal velocity of active filling
EDVI
indexed end‐diastolic volume
IQR
interquartile range
ISACHC
international Small Animal Cardiac Health Council
LA
left atrium
nLVIDd
normalized left ventricular internal diameter in diastole
MM
m‐mode
NT‐proBNP
n‐terminal probrain natriuretic peptide
PA
pulmonary artery
PV
pulmonary vein
PV/PA
pulmonary vein to pulmonary artery ratio
SD
standard deviation
TR
tricuspid regurgitation
VHS
vertebral heart score

Degenerative mitral valve disease (DMVD) is the most common form of acquired heart disease in dogs.1 The course of the disease is highly variable with most dogs spending several years in an asymptomatic state. Approximately one‐third of affected dogs develop congestive heart failure (CHF) and die.2 Early recognition of CHF is important because it enables initiation of conventional therapy in accordance with expert recommendations,3 the avoidance of life‐threatening situations, and limitation of hospitalization costs. Clinical variables, such as resting respiratory rate, can predict cardiac decompensation,4 but no clinical sign is CHF‐specific. Thoracic radiography is considered the clinical “gold standard”, but important interreader variability exists. Diagnosis of CHF should not rely solely on radiographic determination of pulmonary edema.5, 6 The N‐terminal probrain natriuretic peptide (NT‐proBNP) biomarker may contribute to the diagnosis of CHF. The NT‐proBNP concentration correlates with DMVD severity7, 8 and has been shown to help distinguish dogs with respiratory signs caused by CHF from those with respiratory disease.9, 10 However, a wide overlap exists between asymptomatic dogs and dogs in CHF,9, 11, 12 and a small proportion of healthy dogs have high concentrations of NT‐proBNP.13 Furthermore, day‐to‐day14 and interbreed15 variations exist. Doppler echocardiography may confirm DMVD diagnosis and detect dogs in CHF. In dogs with DMVD, peak E to isovolumic relaxation time ratio and diastolic functional class are sensitive markers of CHF.16 However, these measurements require operator expertise. All of these single diagnostic tools have their own limitations, and obtaining a definitive diagnosis often relies on a combination of test results.10, 16 Therefore, the development of an additional, easy‐to‐measure, echocardiographic index might improve early recognition of CHF in dogs.

The pulmonary vein‐to‐pulmonary artery ratio (PV/PA) is a novel echocardiographic variable that might distinguish dogs in CHF from those in a compensated state.17 In dogs with progressive DMVD leading to CHF, we would anticipate that increased pulmonary venous pressure associated with venous enlargement would cause an increase in the PV/PA ratio. Therefore, we first established normal values for PV, PA, and the PV/PA ratio in control dogs of various sizes and determined the correlation between the PV/PA ratio assessed by bidimensional (2D) and M‐mode (MM) echocardiography. Thereafter, we assessed the effect of the class of heart failure on this ratio and determined if PV/PA could be used to identify CHF in dogs with DMVD.

Material and Methods

Dogs

Control and diseased dogs were prospectively recruited (from October 2011 until May 2015) at the Clinical Veterinary Hospital of the University of Liège. Inclusion criteria for affected dogs included the presence of a typical heart murmur and an echocardiographic diagnosis of DMVD, characterized by degenerative changes of the mitral valve leaflets, mitral valve prolapse and the presence of systolic regurgitant flow.18 Control dogs were recruited during routine consultations or belonged to staff members. A thorough history was taken for all dogs before physical examination and Doppler echocardiography. Dogs with evidence of a concomitant disease were excluded. Dogs with DMVD were classified into class I, II, or III according to the International Small Animal Cardiac Health Council (ISACHC) classification system.19 Class I was defined as subclinical heart disease without (IA) or with (IB) evidence of left cardiomegaly. Class II was defined as mild to moderate CHF in the presence of radiographic abnormalities consistent with pulmonary edema. Class III was defined as overt, severe CHF.

Doppler Echocardiography

Transthoracic 2D, MM echocardiography and conventional Doppler echocardiography were performed by 2 trained observers (1 board‐certified cardiologist [KME] and 1 cardiology resident [ACM] using an ultrasound unit1 equipped with 2.2–3.5 and 5.5– 7.5 MHz phased‐array transducers. Dogs were placed in right and left lateral recumbency and a simultaneous 1‐lead electrocardiogram was recorded. Standard right parasternal (long and short axis) and left apical parasternal views were used for data acquisition. Left atrial size was assessed in the right parasternal long axis view using the septal‐to‐caudal dimension of the left atrium (LAmin) and in right parasternal short axis at the level of the aorta (LA). Both measurements were taken at the end of systole and were normalized using aortic diameter (Ao) (LAmin/Ao and LA/Ao, respectively). End‐diastolic left ventricular volume was calculated from the right parasternal long axis view using modified Simpson's rule20 and indexed to body surface area (EDVI). Left ventricular end‐diastolic diameter was measured in MM from a left ventricular short axis view at the level of chordae tendinae and indexed to body weight using the Cornell method (nLVIDd).21 Mitral inflow was recorded using pulsed‐wave Doppler from the left apical parasternal 4‐chamber view (E, A and E/A). The tricuspid valve was systematically evaluated for evidence of regurgitation. If a tricuspid regurgitant (TR) jet was visible, its velocity was recorded and transformed into a pressure gradient according to the modified Bernoulli equation. Pulmonary hypertension was defined as a TR pressure gradient >36 mmHg (TR > 3 m/s). All measurements were performed off‐line by a single trained investigator (KME) blinded to clinical signs and thoracic radiographic scores.

Acquisition and Reproducibility of PV/PA Ratio

For PV/PA measurement, a right parasternal long axis 4‐chamber view was optimized to simultaneously visualize a longitudinal section of the medial PV and the right PA in cross‐section and recorded. Measurements of PV and PA diameters were taken in MM as previously described17 and in 2D. Dimensions were obtained by tracing a line perpendicular to the medial PV and passing through the center of the adjacent right PA. For both measurements, we used the inner edge‐to‐inner edge method at the end of the T wave (Fig (Fig1).1). Three measurements were averaged. Interobserver variability of PV/PA image acquisition and of PV/PA measurement were assessed separately in 10 and 20 dogs, respectively.

Figure 1
Illustration of pulmonary vein diameter (*) and right pulmonary artery diameter (**) measurements in bidimensional (A) and M‐mode (B) using the inner edge to inner edge method.

Thoracic Radiography

Thoracic radiographs (right lateral, left lateral, and dorso‐ventral projections) were taken in a subset of dogs. A board‐certified radiologist (GB) and a radiologist with extensive expertise (ALE) reviewed the images independently. All studies were randomly ordered and radiologists were blinded to the animal's identification, the date of examination, the initial interpretation of images, and the echocardiographic findings. A radiographic composite CHF score was assigned to each patient. This score was based on 3 main criteria: evidence of cardiomegaly, lung pattern, and pulmonary venous congestion. Specifically, cardiomegaly criteria were subdivided as follows: qualitative assessment of the cardiac silhouette, vertebral heart score,22 and presence of left atrial enlargement. A cardiomegaly score of 0 was given when the cardiac silhouette was considered normal; a score of 1 was given when moderate cardiomegaly was present; and, a score of 2 was given when severe cardiomegaly was present. Lung pattern was assessed with a score varying from 0 to 3, with 0 corresponding to a normal lung pattern, 1 to a moderate to severe interstitial pattern, 2 to a localized alveolar pattern, and 3 to a diffuse alveolar pattern. Venous congestion also was assessed with a score between 0 and 2, with 0 corresponding to the absence of venous congestion, 1 for moderate venous congestion (not evident in all pulmonary veins), and 2 for severe venous congestion. A final cumulative score between 0 and 7 was allocated to each dog. The final radiographic assessment also determined if CHF was present or absent. Results of the 2 radiologists were compared and, if there was disagreement with regard to the presence or absence of CHF, images were reviewed by both radiologists together to provide a final consensus.

Statistical Analysis

Statistical analysis was performed using the xlstat2 software. Statistical significance was set at P < .05. Normality was tested using the Shapiro–Wilk test. Continuous data were expressed as median values with interquartile range (IQR, 25th to 75th percentiles). The interobserver variability of image acquisition and measurements was calculated using coefficients of variation (CV) with the formula: CV = standard deviation of measurement/mean of measurement × 100 (%). The PV/PA measurements obtained from 2D and MM were compared using a paired Mann–Whitney log rank test. Nonlinear regression analyses were used to identify and quantify relationships between PV or PA and body weight. Proportions were compared by a chi‐squared test. A Mann–Whitney log rank test was used to compare continuous demographic data between control and affected dogs, and PV/PA between asymptomatic and CHF dogs. Pearson or Spearman correlations were used, according to data distribution, to test the strength of the relationship between PV/PA measured by the 2 methods and other echocardiographic parameters. A Kruskal–Wallis test with Bonferroni's adjustment (corrected P value .005) was used to compare PV/PA among classes of heart failure. Receiver‐operating characteristic (ROC) curve analysis was used to determine the diagnostic accuracy of PV/PA and to define cut‐off values for each prediction.

Results

Dogs

Ninety‐eight dogs were enrolled in the study including 37 controls and 61 dogs with DMVD. In the control group, the most common breeds were crossbreed (7), Jack Russell Terrier (4), Beagle (3), Dachshund (3), West Highland white terrier (3), American Staffordshire (2), Labrador (2), and Yorkshire terrier (2). In dogs with DMVD, the most common breeds were Cavalier King Charles Spaniel (14), crossbreed (7), Jack Russell terrier (5), Bichon Frise (5), Beagle (4), Dachshund (3), Shih‐Tzu (3), Chihuahua (3), Lhasa Apso (2), and Yorkshire terrier (2). Control dogs (6.1 years old; range, 3.9‐9.6) were significantly younger and included more females (21/37) than affected dogs (10 years old; range, 8.6–12.3; P = .001; female, 22/61; P = .045). No significant difference was found for body weight. Demographic data, historical findings, and physical examination results for the DMVD dogs are summarized in Table 1. At presentation, 4 (25%) dogs of class IA and 5 (31%) dogs of class IB were being treated with an angiotensin converting enzyme inhibitors (ACEI). One dog of class IB was also receiving pimobendan (6%). In classes II and III, 13 (65%) and 4 dogs (44%), respectively, had received furosemide before examination. Fifteen (75%) dogs in class II and 4 (44%) dogs in class III were being treated with an ACEI, whereas 10 (50%) and 2 (22%) dogs were receiving pimobendan. Three (15%) dogs in class II and 2 (22%) dogs in class III had received spironolactone.

Table 1
Demographic data, history findings, and the results of physical examination in 61 dogs with degenerative mitral valve disease

PV/PA: Variability and Reference Intervals

Interobserver CV for image acquisition of PV, PA and PV/PA were, respectively, 8, 4 and 8% in 2D and 4, 5 and 7% in MM. Interobserver CV for PV, PA and PV/PA measurements were, respectively, 7, 9 and 9% in 2D 6, 9 and 9% in MM.

In healthy dogs, PV and PA measured in 2D and MM were correlated with body weight; no correlation was found for PV/PA (Fig (Fig2).2). No significant difference was observed between PV/PA obtained in 2D or MM and both methods were highly correlated (r = 0.90; P < .001; Fig Fig3).3). The PV/PA ratio for control dogs was 1.01 (range, 0.95–1.06) in 2D and 1.01 (range, 0.95–1.12) in MM.

Figure 2
Scatter plots of BW to PV diameter measured in 2D (A) and MM (C) and BW to PA diameter measured in 2D (B) and MM (D) in 37 healthy dogs. 2D, bidimensional; BW, Body weight; MM, M‐mode; PA, Pulmonary artery; PV, Pulmonary vein.
Figure 3
Scatter plots of PV/PA ratio obtained in 2D and MM illustrating high correlation between both methods. r = 0.90; P < .001. 2D, bidimensional; MM, M‐mode; PV/PA, pulmonary vein diameter to pulmonary artery diameter.

PV/PA and Class of Heart Failure

The PV/PA ratio increased with class of heart failure (Fig (Fig4).4). The PV/PA ratio was significantly lower in control dogs compared to dogs with DMVD in ISACHC class IB (2D, 1.63; range, 1.42–1.76; MM, 1.60; range, 1.34–1.82), II (2D, 2.36; range, 1.89–2.53; MM, 2.02; range, 1.96–2.35) and III (2D, 2.55; range, 2.24–2.83; MM, 3.07; range, 1.78–3.43; P < .001). The PV/PA also was lower in DMVD dogs in class IA (2D, 1.23; range, 1.14‐ 1.40; MM, 1.15; range, 1.01–1.35) compared to dogs in class II (P < .001) and III (P = .001). Doppler‐echocardiographic variables according to ISACHC class are summarized in Table 2. Regardless of the mode, PV/PA was correlated with other echocardiographic indices of left ventricular filling pressures such as the indexed left atrial and left ventricular sizes and the E/A ratio (Table 3).

Figure 4
Box plot illustrating PV/PA ratio in bi‐dimensional mode (A) and M‐mode (B) according to heart failure classes. The whiskers indicate the range of values. The box contains the 25th–75th centiles. The line within the box indicates ...
Table 2
Echocardiographic variables for 61 dogs and radiographic scores for 43 dogs with different classes of heart failure
Table 3
Correlation between PV/PA and other echocardiographic parameters

PV/PA and Radiographic Score

Thoracic radiographs were available for 13 controls and 43 (70%) dogs with DMVD. A radiographic diagnosis of CHF was made in 39% of dogs with DMVD. The 13 control dogs had no evidence of CHF and had a total radiographic score of 0, 1, or 2. Dogs with a radiographic diagnosis of CHF had higher PV/PA ratios (2D, 2.39; range, 1.89–2.56; MM, 2.16; range, 1.86–2.63) than dogs without CHF on thoracic radiographs (2D, 1.19; range, 1.02–1.41; MM, 1.09; range, 0.96–1.35; P < .001; Fig Fig5).5). The PV/PA was significantly correlated with radiographic composite score (2D, r = 0.66; P < .001; MM, r = 0.65; P < .001).

Figure 5
Median and scatter plots of PV/PA measured in 2D (A) or in MM (B) in 19 dogs with compensated DMVD and in 23 dogs with DMVD and radiographic evidence of CHF. White dots correspond to dogs with evidence of mild to moderate pulmonary hypertension (pressure ...

The receiver operating characteristic (ROC) curve analysis indicated that the PV/PA ratio measured in 2D and MM was useful to distinguish dogs with CHF (diagnosed by radiography) from dogs without CHF (Fig (Fig6).6). Area‐under‐the receiver‐operating characteristic curves (AUC) was 0.98 (CI, 0.97–0.98) for both 2D and MM. A cut‐off value of 1.7 in 2D and 1.6 in MM predicted CHF in DMVD dogs with sensitivity of 96% (CI, 77–100) and 87% (CI, 67–96) and specificity of 91% (CI, 75–97) and 94% (CI, 79–99), respectively.

Figure 6
Receiver‐operating characteristic (ROC) curve illustrating PV/PA ratio measured in 2D (A) and M‐Mode (B) that distinguishes asymptomatic from decompensated dogs with degenerative mitral valve disease. 2D, bidimensional; AUC, Area under ...

Discussion

The diagnosis of CHF in dogs with DMVD relies on a combined interpretation of clinical signs and results of ≥1 diagnostic tools including thoracic radiography, NT‐proBNP or Doppler echocardiography. Pulmonary venous congestion suggests increased pulmonary venous pressure, the trigger factor for development of pulmonary edema. The principal finding of our study is that, PV/PA, a new echocardiographic index of pulmonary venous congestion, increases with heart failure severity in dogs with DMVD. Using a cut‐off value of 1.7 in 2D, PV/PA differentiates dogs with CHF from dogs without CHF with high accuracy using thoracic radiographs as the gold standard.

Interobserver Variability and Normal Values of PV/PA

Assessment of observer variability is an essential step in the evaluation of the diagnostic value of a measurement. The interobserver CV for image acquisition and measurements ranged from 4 to 8% which indicated good to excellent reproducibility. We also found that both measurement methods were highly correlated. In our study, suggested reference values for the PV/PA ratio in healthy dogs were approximately 1 in 2D and MM, based on data from 37 dogs. These reference ranges are in accordance with a previous report describing a mean PV/PA ratio of 1 in MM with SD of 0.11.17 A PV/PA value of 1 might therefore be used in future studies as a reference value for healthy dogs.

Diagnosis of CHF by PV/PA

Increased left ventricular filling pressure is a key feature of left‐sided CHF, with therapeutic and prognostic significance, in dogs with DMVD. However, this hemodynamic variable cannot be directly measured in a noninvasive manner prompting a search for accurate surrogate markers. As DMVD progresses, the left atrium and ventricle gradually enlarge. Subsequently, left atrial pressure, which is dependent on the regurgitant volume, chamber compliance and pulmonary venous pressure, increases. This increase can lead, in a subset of dogs with DMVD, to left‐sided CHF. In dogs with DMVD, left atrial size, as assessed by the ratio of LA/Ao, has been shown to increase with increasing class of heart failure,23 to be associated with decreased survival time2, 7, 24 and to predict CHF.16, 24 A similar scenario might be expected for pulmonary vein size, but this has not been studied in dogs. We anticipate that in the absence of congenital heart disease (left‐to‐right shunt or pulmonic stenosis) and in the absence of pulmonary hypertension, the right pulmonary artery diameter should be relatively stable. Therefore, in uncomplicated DMVD, an increase of PV diameter (and PV/PA) is expected. In this study, PV/PA increased with increasing class of heart failure. Furthermore, dogs identified to be in CHF by thoracic radiography had higher PV/PA values than did asymptomatic dogs. The increase of PV/PA is likely related to pulmonary vein enlargement. This variable had an AUC >0.9 indicating excellent diagnostic performance for CHF detection. A PV/PA value >1.7 in 2D predicted CHF with an accuracy >90%, a sensitivity of 96%, and a specificity of 91%. This variable had an AUC > 0.9, indicating excellent diagnostic performance for CHF detection. Theoretically, the presence of concurrent pulmonary hypertension could contribute to false negative results by decreasing the PV/PA ratio. In our study, the presence of pulmonary hypertension did not interfere with CHF diagnosis performed by echocardiographic assessment of PV/PA. Other factors that might influence PV/PA values include an absence of venous congestion as sometimes is observed in acute CHF caused by chordae tendinae rupture, differences in pulmonary venous tone or compliance, and the presence of an abnormally small or large PA.

Comparison with Other Doppler‐echocardiographic Indices of CHF

Each Doppler‐echocardiographic‐derived surrogate measure for left ventricular filling pressure has its own limitations. Evaluation of filling pressures depends on an integrated interpretation of volume overload remodeling indices as well as indices of left ventricular filling. In our study, PV/PA correlated with left atrial and ventricular size, echocardiographic parameters of volume overload already known to identify CHF.24 We also showed that in dogs with DMVD, PV/PA was correlated with mitral E/A ratio. Transmitral flow patterns may identify diastolic dysfunction and increased left ventricular filling pressures, but these variables are interrelated.25 The diagnostic accuracy of E/A for CHF detection may be limited in dogs with DMVD because of the large influence of volume overload on left ventricular filling. Furthermore, the influence of preload on early diastolic tissue velocity (E′) in mitral regurgitation explains why identification of CHF using the E/E′ ratio is less accurate in DMVD than in dilated cardiomyopathy.16 The ratio between E and the isovolumetric relaxation time has been shown to be the optimal Doppler index for identifying CHF in dogs with DMVD.16 However, these measurements require Doppler mode and a high level of expertise, and can prolong the duration of echocardiographic examination. On the contrary, the PV/PA ratio is relatively simple to obtain by conventional 2D or MM echocardiography.

Limitations

This study has several limitations. Left ventricular filling pressures were not directly measured and thoracic radiography was used as the gold standard to assess the presence or absence of CHF. The sensitivity and specificity of thoracic radiography are unknown and are observer dependent.5, 6 Second, a majority of dogs in CHF were on treatment to decrease venous pressure before their examination, and this could have confounded interpretation of both Doppler‐echocardiographic variables and thoracic radiographs. Third, the relatively small population studied rendered the study underpowered especially for comparison of PV/PA among classes of heart failure. Additional studies evaluating the accuracy of PV/PA to predict CHF in DMVD dogs using the pre‐established cut‐off value are indicated.

Conclusion

Despite these limitations, our study has demonstrated that PV/PA is a simple and reproducible echocardiographic variable that can be obtained by 2D or MM echocardiography. In DMVD dogs, PV/PA increases with increasing stage of heart failure. Using a cut‐off value of 1.7 in 2D, PV/PA permitted accurate discrimination of dogs in CHF from asymptomatic dogs. Additional studies are required to determine if PV/PA might provide additional information in the integrated interpretation of Doppler‐echocardiographic indices of left ventricular filling pressure. This measure also could have potential value in the rapid assessment of CHF in dogs in a critical care setting.

Acknowledgments

Conflict of Interest Declaration: Authors disclose no conflict of interest.

Off‐label Antimicrobial Declaration: Authors declare no off‐label use of antimicrobials.

Footnotes

1Vivid I, General Electric Medical System, Waukesha, WI

2Xlstat 2014.6.01, Addinsoft, Paris, France

References

1. Buchanan JW. Chronic valvular disease (endocardiosis) in dogs. Adv Vet Sci Comp Med 1977;21:75–106. [PubMed]
2. Borgarelli M, Savarino P, Crosara S, et al. Survival characteristics and prognostic variables of dogs with mitral regurgitation attributable to myxomatous valve disease. J Vet Intern Med e 2008;22:120–128. [PubMed]
3. Atkins C, Bonagura J, Ettinger S, et al. Guidelines for the diagnosis and treatment of canine chronic valvular heart disease. J Vet Intern Med 2009;23:1142–1150. [PubMed]
4. Schober KE, Hart TM, Stern JA, et al. Effects of treatment on respiratory rate, serum natriuretic peptide concentration, and Doppler echocardiographic indices of left ventricular filling pressure in dogs with congestive heart failure secondary to degenerative mitral valve disease and dilated cardiomyopathy. J Am Vet Med Assoc 2011;239:468–479. [PubMed]
5. Hansson K, Haggstrom J, Kvart C, et al. Reader performance in radiographic diagnosis of signs of mitral regurgitation in cavalier King Charles spaniels. J Small Anim Pract 2009;50(Suppl. 1):44–53. [PubMed]
6. Henriksson L, Sundin A, Smedby O, et al. Assessment of congestive heart failure in chest radiographs. Observer performance with two common film‐screen systems. Acta Radiol 1990;31:469–471. [PubMed]
7. Serres F, Pouchelon JL, Poujol L, et al. Plasma N‐terminal pro‐B‐type natriuretic peptide concentration helps to predict survival in dogs with symptomatic degenerative mitral valve disease regardless of and in combination with the initial clinical status at admission. J Vet Cardiol 2009;11:103–121. [PubMed]
8. Moesgaard SG, Falk T, Teerlink T, et al. Brain‐natriuretic peptide and cyclic guanosine monophosphate as biomarkers of myxomatous mitral valve disease in dogs. Vet J 2011;189:349–352. [PubMed]
9. Fine DM, DeClue AE, Reinero CR. Evaluation of circulating amino terminal‐pro‐B‐type natriuretic peptide concentration in dogs with respiratory distress attributable to congestive heart failure or primary pulmonary disease. J Am Vet Med Assoc 2008;232:1674–1679. [PubMed]
10. Fox PR, Oyama MA, Hezzell MJ, et al. Relationship of plasma N‐terminal pro‐brain natriuretic peptide concentrations to heart failure classification and cause of respiratory distress in dogs using a 2nd generation ELISA assay. J Vet Intern Med 2015;29:171–179. [PubMed]
11. MacDonald KA, Kittleson MD, Munro C, et al. Brain natriuretic peptide concentration in dogs with heart disease and congestive heart failure. J Vet Intern Med 2003;17:172–177. [PubMed]
12. Tarnow I, Olsen LH, Kvart C, et al. Predictive value of natriuretic peptides in dogs with mitral valve disease. Vet J 2009;180:195–201. [PubMed]
13. Misbach C, Chetboul V, Concordet D, et al. Basal plasma concentrations of N‐terminal pro‐B‐type natriuretic peptide in clinically healthy adult small size dogs: Effect of body weight, age, gender and breed, and reference intervals. Res Vet Sci 2013;95:879–885. [PubMed]
14. Kellihan HB, Oyama MA, Reynolds CA, et al. Weekly variability of plasma and serum NT‐proBNP measurements in normal dogs. J Vet Cardiol 2009;11(Suppl. 1):S93–S97. [PubMed]
15. Sjostrand K, Wess G, Ljungvall I, et al. Breed differences in natriuretic peptides in healthy dogs. J Vet Intern Med 2014;28:451–457. [PubMed]
16. Schober KE, Hart TM, Stern JA, et al. Detection of congestive heart failure in dogs by Doppler echocardiography. J Vet Intern Med 2010;24:1358–1368. [PubMed]
17. Birettoni F, Caivano D, Giorgi ME, et al. A Novel Echocardiographic Index in the Dog: Pulmonary Vein: Pulmonary Artery Diameter. Presented at the 20th Annual ECVIM‐CA congress Toulouse (abstract).
18. Chetboul V, Tissier R. Echocardiographic assessment of canine degenerative mitral valve disease. J Vet Cardiol 2012;14:127–148. [PubMed]
19. Fox PR, Sisson D, Moïse NS Textbook of Canine and Feline Cardiology. Principles and Clinical Practice. 2nd ed Philadelphia: Saunders Company; 1999.
20. Dukes‐McEwan J, et al. Proposed guidelines for the diagnosis of canine idiopathic dilated cardiomyopathy. J Vet Cardiol 2003;5:7–19. [PubMed]
21. Cornell CC, Kittleson MD, Della Torre P, et al. Allometric scaling of M‐mode cardiac measurements in normal adult dogs. J Vet Intern Med 2004;18:311–321. [PubMed]
22. Buchanan JW, Bucheler J. Vertebral scale system to measure canine heart size in radiographs. J Am Vet Med Assoc 1995;206:194–199. [PubMed]
23. Borgarelli M, Haggstrom J. Canine degenerative myxomatous mitral valve disease: Natural history, clinical presentation and therapy. Vet Clin North Am Small Anim Pract 2010;40:651–663. [PubMed]
24. Reynolds CA, Brown DC, Rush JE, et al. Prediction of first onset of congestive heart failure in dogs with degenerative mitral valve disease: The PREDICT cohort study. J Vet Cardiol 2012;14:193–202. [PubMed]
25. Bonagura JD, Schober KE. Can ventricular function be assessed by echocardiography in chronic canine mitral valve disease? J Small Anim Pract 2009;50(Suppl. 1):12–24. [PubMed]

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