Vascular permeability and ICAM-1 expression of lung tissues. (A, B) The contents of total protein (A) and TNF-α (B) in BAL fluids collected from rats in Figure 1 were measured. (C, D) The expression of ICAM-1 in lung tissues were examined by immunohistochemistry (C) and Western blot analysis (D). The density of each band was normalized to GAPDH. *P < 0.05 and **P < 0.001 indicate a significant difference from sham-operated rats. ‡P < 0.05 and ‡‡P < 0.001 indicate a significant reduction from vehicle-treated IAC rats.