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Effects of Telmisartan on Perirenal Adipose Tissue and Kidney Function in Obese Rats Induced by a High-Fat Diet
Li Hao,1 Wang Ya-ping,1 Song Yan,1 Zhang Heng,1 Li Min,1 Suo Meng,1 Gao Lan,1 and Tian Gang1
1Department of Critical Care Medicine, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an Shaanxi, China. Correspondence: Tian Gang (nc.ude.utjx@gnagnait).
Objective: To study the effects of telmisartan on perirenal adipose tissue and renal function in obese rats induced by high-fat diet, and the underlying mechanisms.
Methods: Male Wistar rats fed with high-fat or standard diet for 12 weeks were randomly divided into high-fat diet, high-fat plus telmisartan treatment (8mg/kg·d), standard diet and standard diet plus telmisartan treatment groups (8mg/kg·d, n = 7 per group). After 20 weeks of telmisartan or vehicle treatment, perirenal adipose tissue and renal functions were examined. Glomerular endothelial cells and glomerular mesangial cells were cultured with perirenal adipose tissue-conditioned media, and cell proliferation was assessed by MTT assay.
Results: Compared to the standard diet group, the levels of serum urea nitrogen, creatinine, 24-hour urinary protein were significantly higher in the high-fat diet group (all P < 0.05). High-fat diet also increased the expression of leptin, TNF-α, MCP-1, and IL-6, while decreased PPAR-γ, adipocyte fatty acid binding protein (aP2), and adiponectin expression in perirenal adipose tissues (all P < 0.05). These changes were restored by telmisartan treatment (all P < 0.05). Furthermore, conditioned media from perirenal adipose tissues significantly increased cell proliferation of glomerular endothelial cells and glomerular mesangial cells in the high-fat diet group compared to standard diet group (all P < 0.05), which was prevented by telmisartan treatment (all P < 0.05).
Conclusion: High-fat diet induces dysfunctions of perirenal adipose tissues and the kidney in Wistar rats, which can be prevented by telmisartan treatment. Our study highlights perirenal adipose tissue as a target of telmisartan treatment.