PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of interfaceThe Royal Society PublishingInterfaceAboutBrowse by SubjectAlertsFree Trial
 
J R Soc Interface. 2016 April; 13(117): 20151083.
PMCID: PMC4874429

Microscopic and infrared spectroscopic comparison of the underwater adhesives produced by germlings of the brown seaweed species Durvillaea antarctica and Hormosira banksii

Abstract

Adhesives from marine organisms are often the source of inspiration for the development of glues able to create durable bonds in wet environments. In this work, we investigated the adhesive secretions produced by germlings of two large seaweed species from the South Pacific, Durvillaea antarctica, also named ‘the strongest kelp in the word’, and its close relative Hormosira banksii. The comparative analysis was based on optical and scanning electron microscopy imaging as well as Fourier transform infrared (FTIR) spectroscopy and principal component analysis (PCA). For both species, the egg surface presents peripheral vesicles which are released soon after fertilization to discharge a primary adhesive. This is characterized by peaks representative of carbohydrate molecules. A secondary protein-based adhesive is then secreted in the early developmental stages of the germlings. Energy dispersive X-ray, FTIR and PCA indicate that D. antarctica secretions also contain sulfated moieties, and become cross-linked with time, both conferring strong adhesive and cohesive properties. On the other hand, H. banksii secretions are complemented by the putative adhesive phlorotannins, and are characterized by a simple mechanism in which all constituents are released with the same rate and with no apparent cross-linking. It is also noted that the release of adhesive materials appears to be faster and more copious in D. antarctica than in H. banksii. Overall, this study highlights that both quantity and quality of the adhesives matter in explaining the superior attachment ability of D. antarctica.

Keywords: adhesive, brown algae, microscopy, ATR-FTIR, principal component analysis

1. Introduction

Adhesive formulations and designs inspired from Nature represent the new frontier for the production of smart, green, biocompatible and sustainable adhesives and sealants. Biological adhesives can be exploited in a vast range of industries including defence, naval, biomedical, biosensor and surgical applications [1]. For example, devices mimicking the attachment of geckos are now a reality in climbing robots [2], whereas biological adhesives derived from mussels are under investigation as surgical tissue adhesive [3]. For these reasons, research interest in biological adhesives is increasingly receiving attention from both the academic and the industrial worlds, in the search for both new ‘sticky’ organisms and new applications [4,5].

Nature offers an extensive range of biological species with adhesive capabilities that can be sourced to seek ideas and inspiration. For example, underwater adhesion is very elegantly achieved by a multitude of organisms such as mussels [6], barnacles [7], sandcastle worms [8], sea urchins [9] and starfishes [10], to name a few. All these species are able to firmly adhere in wet environments, either temporary or irreversibly, an achievement that humans still struggle to successfully mimic.

The attachment achieved by brown algae is particularly fascinating. For example, adult plants of the giant kelps Macrocystis pyrifera and Nereocystis luetkeana [11] and the fucoid Durvillaea antarctica [12] can easily be longer than 10 m and weigh more than 50 kg. They are able to produce a strong, underwater, irreversible and long-lasting attachment with a surface able to withstand the severe hydrodynamic drags associated with wave action and tidal currents [13,14]. These features make brown algae especially interesting in the bioadhesion arena. In particular, D. antarctica and Hormosira banksii thrive in the intertidal zone, i.e. in wave-exposed areas where the hydrodynamic drag is highest. These two species are closely related, belonging to the most evolutionally developed species within the class of the Phaeophyceae. They display characteristic dichotomous reproduction, i.e. sexually distinct plants releasing separate male (sperm) and female (eggs) gametes, with consequent production of a zygote upon mating of the two gametes [15]. However, the two plants are distinguished, among other features, by their different location in the intertidal habitat and in their size: while H. banksii is relatively small, with an average size of 40 cm, and mostly present in relatively wave-sheltered locations [16,17], D. antarctica can be as long as 10 m and thrives only in extremely wave-exposed areas [12,18]. This observation leads to the postulate that the two species have different attachment mechanisms. Interestingly, Stevens et al. [19] suggested that D. antarctica might be ‘the strongest kelp in the world’ because of its particularly high attachment strength and the mechanical properties of its thallus. Taylor & Schiel [20] carried out basic adhesion studies on brown algae zygotes to explain ecological differences in gamete dispersal and zygote settlement, demonstrating that attachment of D. antarctica zygotes is greater than that presented by H. banksii even in the early stages of their life cycles.

The use of zygotes for the determination of the adhesion characteristics of seaweed is particularly interesting. While adult seaweeds cling onto surfaces by a combination of mechanical (interlocking with surface asperities in the millimetre scale) and chemical interactions, zygotes must primarily rely on chemically based adhesion. For example, Dimartino et al. [21] employed a laminar flow cell to estimate the adhesion strength of settled zygotes of H. banksii while excluding the mechanical component of their attachment. In addition, even though gametes do not have adhesive abilities, the secretion of bioadhesive components is immediately triggered upon fertilization to maximize survival rates in the harsh natural environment. For these reasons, zygotes represent an ideal system to further study the attachment characteristics of the glue produced by large seaweed.

Different methods have been used to determine the structural characteristics as well as the chemical composition of biological adhesives from seaweed species, including optical [22,23] and electron microscopy (scanning electron microscopy (SEM) [2426], environmental SEM [27] and transmission electron microscopy [23,28,29]), atomic force microscopy [3032], quartz crystal microbalance with dissipation [33,34], zeta potential [35], energy dispersive X-ray (EDX) [29,30], chemical extraction [36,37], enzymatic assays [38,39], staining and labelling techniques [23,24,40] and infrared spectroscopy [29]. The preparation protocols as well as the testing procedure of most methods require specific conditions often harmful to the biological sample tested and in most cases lead to the death of the specimen, hence the information obtained is usually a snapshot of the sample at a certain point in time. Among others, attenuated total reflection–Fourier transform infrared (ATR-FTIR) spectroscopy is an effective technique to study biological adhesives, probing only a thin layer of the sticky materials deposited on the optical ATR element. ATR-FTIR is particularly interesting as it is a non-invasive technique that permits the analysis of biological samples under conditions resembling their natural environment, making it possible to maintain the physiological requirements necessary for cell survival. The opportunity offered by ATR-FTIR to study the production of bioadhesives from complex biological species in real time, in vivo and in situ, has been clearly recognized by two recent reviews by Barlow & Wahl [41] and Petrone [42]. For example, ATR-FTIR has been successfully employed to identify the composition of adhesives from a range of biological species including barnacles [43], bacteria [44], diatoms [30], algae [29] and mussels [45].

In general, brown algal bioadhesives are a complex mixture of different organic compounds, including proteins, carbohydrates, glycoproteins, polyhydroxyphenols and metal ions, mutually interacting through cross-linked bonds as well as electrostatic forces and metal ion bridge complexes [30,4650]. Most of the organic compounds listed above have been associated with the adhesive simply because they are secreted after fertilization, even though the same compounds probably have other important physiological and ecological roles, e.g. in the formation of the cell wall, prevention of polyspermy, detention of grazing predators, etc. For example, Bitton & Bianco-Peled [51] demonstrated that oxidation and cross-linking mediated by a haloperoxidase enzyme is not strictly required to achieve strong adhesion bonds, questioning the role of the enzyme in algal attachment.

In this work, we have studied and compared the adhesion of recently fertilized zygotes of the two intertidal seaweed species H. banksii and D. antarctica, with the objective of identifying the key features that make the latter the ‘strongest kelp in the word’. Microscopy was initially employed to clarify the mechanism of release of the glue ingredients in kelp zygotes as well as morphological changes in the adhesive pad. EDX spectroscopy was also considered as a tool to identify specific elements in the attaching holdfast. ATR-FTIR spectroscopy was then employed to characterize the chemical constituent present in the adhesive secretions. Principal component analysis (PCA) was finally applied to the spectroscopic results to find small sources of variance in the spectral features associated with the chemical components involved in the attachment process. PCA is a widely used technique in investigative spectroscopic analysis [5255]; however, at this point in time it has been used minimally in the field of bioadhesives.

2. Material and methods

2.1. Sample collection and preparation of gamete suspensions

The procedure employed to harvest suspensions of gametes from H. banksii and D. antarctica reflects the protocol described by Dimartino et al. [56]. Briefly, fertile fronds from mature plants were collected at Shag Point, New Zealand (−45° 27′ 48″, 170° 49′ 20″), in August 2013, transported in a chilly bin and thoroughly washed with 2 µm filtered and UV-treated seawater. Each frond was stored in a separate plastic bag and kept in the dark at 4°C for a period of between 12 and 48 h, followed by thermal and light shock treatment using two halogen portable floodlights (500 W each) to stimulate gamete release. Sex of the gametes released is determined by visual inspection of the exudates, i.e. white and orange for male specimen of D. antarctica and H. banksii, respectively, and olive brown for female gametes of both species. The various gametes were harvested by washing the blades in separate reservoirs containing sterile seawater at 13°C. The suspensions obtained were filtered through plankton nets (mesh size was 105 and 25 µm for eggs and sperm, respectively) and further clarified by three subsequent sedimentation cycles under gravity. At all times, fronds, labware and suspensions were carefully manipulated to avoid anticipated contact of eggs and sperm leading to uncontrolled fertilization.

2.2. Cultures of germlings

D. antarctica and H. banksii zygotes were produced by simple mixing of the two gamete suspensions for around 30 min. The zygotes were inoculated onto the desired substrate and settled under gravity. The resulting cultures were placed in a temperature-controlled chamber at 13°C with 12 h : 12 h light–dark cycles. The light intensity during the light period was of 23 ± 6 µmol photons m−2 s−1 of photosynthetically active radiation as measured using a 2π quantum sensor coupled to a LI-250A light meter (LI-COR Biosciences, Lincoln, NE). Seawater was changed every 12 h to maintain the culture's viability. This protocol was effective to maintain the cultures up to at least 140 h. All due care has been devoted during sample preparation to avoid bacterial contamination. However, owing to the wild nature of the samples, this could not be completely prevented. Yet, within the relevant time window of the experiments, the observed growth rate of the microorganisms was much less than the development of the zygotes/germlings, thus its influence on the experimental results was negligible.

In the following, the term zygote refers to fertilized eggs in the very early life stages, whereas germlings will be used to denote the zygotes after settling on the slide surfaces as well as at later developmental stages.

2.3. Optical, electron microscopy and energy dispersive X-ray spectroscopy

Cultures on glass and ZnSe slides (Thermo Fisher Scientific, Auckland, New Zealand, and Harrick Scientific, Horizon, Pleasantville, NY, respectively) were monitored at regular intervals up to 140 h after initial settlement under optical microscopy using a Zeiss Axiostar Plus microscope equipped with a Canon Powershot A620 camera (Carl Zeiss, North Ryde, Australia). The slides were carefully removed from the seawater bath to avoid turbulence that could potentially dislodge loosely adhered germlings. A layer of seawater at 13°C was kept on the slides above the germlings during microscopic observation to minimize sample drying and temperature increase. A total of four replicate slides was observed at each time interval. Once imaged, the slides were dismissed to preclude the influence on successive germling development of environmental disturbances introduced during imaging.

Cultures on Thermanox™ coverslips (Thermo Fisher Scientific) were employed for SEM. Four replicate slides of settled eggs as well as of germlings at 1 and 24 h following fertilization were imaged. The samples were fixed in 2.5% gluteraldehyde in seawater, buffered in 0.1% cacodylate and postfixed in 1% osmium tetraoxide (Sigma Aldrich, Castle Hill, Australia). The slides were mounted on aluminium stubs, sputter coated with gold/palladium (Emitech K575X, EM Technologies Ltd, Ashford, UK) and imaged under a JEOL 6700 SEM (JEOL Ltd, Tokyo, Japan) using an accelerating voltage of 3 kV. Preliminary information on the elemental composition of the adhesive pad was obtained using the EDX system fitted in the SEM (JEOL 2300F EDS; JEOL Ltd) at an accelerating voltage of 10 kV.

Different sample preparation methods were employed, including freeze-drying, cryo-SEM (Gatan Alto 2500 cryo stage; Gatan Inc., Pleasanton, CA) and critical point drying (CPD; Bal-Tec CPD-030; Bal-Tec AG, Balzers, Liechtenstein). While all preparation methods consistently produced similar morphological features of the samples imaged, freeze-drying often caused cell breakage and a barely visible adhesive footprint, cryo-SEM resulted in germlings detaching from the SEM stubs, whereas CPD preserved the structure of the attached cells and their adhesive secretions. Accordingly, unless otherwise stated, the images presented in the manuscript have been obtained through CPD.

Features in the microscopy images were measured using ToupView software (v. 3.5.563; ToupTek Photonics, Zhejiang, P.R. China). In the Results and discussion section, the average size of objects is reported ± standard deviation (s.d.).

2.4. Attenuated total reflection–Fourier transform infrared spectroscopy

Spectroscopic measurements were carried out with a Digilab FTS-4000 FTIR spectrometer (Digilab LCC, Randolph, MA) equipped with a KBr beamsplitter and DTGS detector and controlled by Digilab Resolutions software (v. 4.0). A horizon ATR-FTIR optical accessory with a ZnSe 13-reflection 50 × 10 × 2 mm (45°) prism (Harrick Scientific, Pleasantville, NY) was mounted in a custom-built flow cell fitted in a temperature-controlled jacket at 13°C in a similar set-up to that depicted previously [45,57]. The penetration depth of the exponentially decaying evanescent wave into the sample is about 0.7 µm at 1500 cm−1 for the ZnSe/seawater interface. All spectra were obtained from 64 co-added scans at 4 cm−1 resolution measured at regular time intervals.

The solutions were fed to the ATR flow cell at a flow rate of 2 ml min−1 using a peristaltic pump placed upstream of the ATR measuring chamber. A spectral background was acquired in an initial equilibration step with flowing seawater at 13°C for 30 min. In the presented absorbance spectra, which may be regarded as difference spectra, the seawater absorptions in both sample and background spectra are effectively removed. After equilibration, 5 ml of the egg suspension was introduced into the flow chamber, where the female gametes settled on the ZnSe surface by gravity forming a relatively uniform monolayer of eggs. Seawater was then flowed for 1 h to allow stable settlement of the eggs on the prism surface, followed by introduction of the sperm suspension for 30 min. Finally, seawater was fed again to wash out excess of sperm, and the first 24 h of germling development was spectrally monitored. It is worth noting here that fertilization is a fast process which occurs in around 10–15 min with high success rates (more than 95%) [58,59]; therefore, it was assumed all viable eggs settled on the prism surface were successfully fertilized.

The potentially noxious effect of ZnSe solutes on the cultures was evaluated using control cultures on glass slides. The cultures were monitored for up to 140 h. Cell division and germling proliferation occurred similarly on both substrates, indicating no apparent toxic effects arising from ZnSe in the early life stages of the germlings.

2.5. Principal component analysis

Principal component analysis (PCA) was carried out on the ATR-FTIR spectra to identify small sources of variance in the spectra. The spectra were first preprocessed using standard normal variate normalization over the spectral region of interest, which was 900–1800 cm−1. PCA was carried out on these normalized spectra with full cross validation for outlier identification. The two species were analysed both separately and together to identify variance within and between the two species. The preprocessing and PCA were carried out using the Unscrambler X v. 10.3 (Camo, Norway).

3. Results and discussion

3.1. Microscopy

The development of H. banksii and D. antarctica germlings was compared under optical microscopy (figure 1) and SEM (figures 2 and and3)3) at different times.

Figure 1.
Optical micrographs of cultured germlings on glass substrates. (ac) D. antarctica at 6, 30 and 140 h after fertilization, respectively. (d–f) H. banksii at 6, 42 and 140 h after fertilization, respectively. The arrows in (c) indicate ...
Figure 2.
SEM images of H. banksii. (a) Cluster of unfertilized eggs displaying numerous peripheral vesicles. (b) Magnification of the polydisperse vesicles covering the eggs' surfaces. (c) Germlings 1 h after fertilization. Remnants of sperms and antheridia (jacket ...
Figure 3.
SEM images of D. antarctica. (a) Unfertilized egg with an irregular surface with crater-like structures. (b) Germlings 1 h after fertilization displaying a smooth surface with scattered surface vesicles. An adhesive footprint composed of an array of small ...

Eggs, zygotes and cultured germlings from D. antarctica are smaller in size than those of H. banksii. In particular, the zygotes have an average diameter of 37 ± 3 µm and 66 ± 1 µm for D. antarctica and H. banksii, respectively. This observation offers a first morphological clue for the stronger attachment (i.e. higher survival) of D. antarctica germlings in wave-exposed environments. In fact, according to Boulbene et al. [60], the hydrodynamic forces experienced by a submerged spherical body scale with the cube of its radius. Thus, H. banksii germlings will experience hydrodynamic stresses one order of magnitude greater than those acting on the comparatively smaller D. antarctica germlings.

Germlings from both species undergo similar growth phases; however, D. antarctica seems to develop faster than its close relative H. banksii. In particular, 6 h old germlings of D. antarctica present apparent polarization and the formation of a rhizoidal tip (figure 1a), whereas the first cellular division occurs between 24 and 36 h after fertilization (figure 1b), with the differentiation of a rhizoidal mother cell and an apical mother cell. After 140 h (6 days), D. antarctica germlings have a prolonged body of variable dimensions approximately 90–360 µm long, i.e. 3–10 times the size of the original zygotes. In a few instances, the thallus detaches from the surface, allowing the germlings to stand upright, with attachment to the substrate secured through the rhizoid only (arrows in figure 1c). On the other hand, H. banksii germlings grow to a lesser extent and show the first signs of polarization only after around 36 h (figure 1e). Cultures 140 h old display germlings still entirely anchored to the substrate, suggesting a less mature rhizoid not able to guarantee a solid attachment to secure the germling. Morphologically, these are characterized by a bulky apical cell of similar dimensions to the zygote (diameter of head 71 ± 4 µm) and a thin rhizoidal prolongation 317 ± 39 µm long (figure 1f). Accordingly, faster physiological development of D. antarctica germlings can be reasonably associated with greater production of adhesive components with respect to H. banksii germlings, a second clue to explain the stronger adhesion of the former species over the latter. This hypothesis will be tested in §3.3.

Careful inspection of microscopic images at higher magnification reveals the presence of a mucilaginous coat surrounding the germlings (electronic supplementary material, figure S1). This film fully develops in the first 24 h following fertilization, reaching a maximum observed thickness of the order of 10 µm. The coat appears more abundant on the rhizoidal tip, especially at later times during germling development. This layer has been described as a water-rich hydrogel composed of a range of broadly defined mucopolysaccharides and polyphenolic material responsible for the attachment of the germlings [61]. The characteristics of such an adhesive hydrogel gradually secreted by the germling will be further discussed in §3.3.

The outer surface of H. banksii eggs (figure 2a,b) presents a number of polydisperse spherical vesicles (diameter 2.4 ± 1.4 µm). The eggs are not surrounded by mucilage, mainly to facilitate interaction with and fertilization by sperms [26]; therefore, the protruding vesicles are unprotected in the outermost layer of the eggs.

Immediately after fertilization, all vesicles must be released and a smooth cell wall is formed [62] (figure 2c). It is possible to observe that both germlings in the image achieve a smooth surface, with one partly covered by remnants of sperm and antheridia following the fertilization step. The release of the peripheral vesicles and formation of a smooth cell wall is a distinctive characteristic in the reproduction of brown algae [63,64]. Tilted SEM images 1 h following fertilization do not reveal signs of adhesive materials linking the germlings to the substrate (micrograph not shown). However, 24 h after fertilization, some isolated connecting threads as well as residual material on the substrate become visible (figure 2d). The threads could be remnants of the adhering hydrogel following the evacuation procedure, possibly indicating a fibrillar structure. Overall, the cell wall remains smooth. These observations suggest that the mucilage formed after fertilization does not leave visible residues after evacuation, indicating that it is a hydrogel extremely rich in water whose spare components collapse uniformly over the germling surface, without altering the smooth appearance of the cell wall.

SEM images of D. antarctica show the same basic features observed on H. banksii. For example, D. antarctica eggs display an irregular surface (figure 3a) which becomes smoother soon after fertilization owing to the formation of a cell wall (figure 3b) [63]. However, distinctive morphological differences are also apparent. In fact, the roughness of D. antarctica eggs is not associated with protruding vesicles; rather, it is associated with peculiar crater-like structures. These may correspond to erupted vesicles as a result of sample preparation. Other SEM images for the eggs show vesicles buried under this external surface, which in this case would represent an outer layer containing the vesicles (electronic supplementary material, figure S2). It is interesting to note that D. antarctica eggs are able to develop a somewhat firm adhesion to substrates even prior to fertilization [65]. Two different hypotheses can be formulated to combine the SEM observations with the preliminary stickiness of the eggs: (i) the vesicles are prone to eruption and release of primary adhesive components or (ii) the outer layer is made of an adhesive substance, and the windows in the shell act as passageways for incoming sperm as well as other biochemical components secreted soon after fertilization.

As early as 1 h after fertilization, the surface of D. antarctica germlings displays a copious number of submicrometric vesicles (diameter 0.26 ± 0.03 µm; figure 3b,c). Germlings observed 96 h after fertilization still exhibit such vesicles. These vesicles are monodisperse in size and uniformly present over the entire germlings' surface, possibly containing precursors of the mucilaginous coat. D. antarctica germlings could have developed a strategy where adhesive vesicles are released in all directions, thus maximizing the adhesion points with the asperities of the natural substrate [66]. In contrast, H. banksii did not display such formations, possibly indicating that the production of the external mucilage is slower or that the hydrogel is weakly bound and washed away during sample preparation. Given the prominent role of the outer mucilage in surface attachment, early adhesion of D. antarctica germlings seems more favoured than those of H. banksii.

A clear adhesive footprint is already visible in the first hours after fertilization (figure 3b). The footprint is constituted by an array of adhesive pads, which are possibly remnants of connective material that changed appearance as a consequence of sample preparation for SEM imaging. Abundant discharge of adhesive materials continues as the germlings develop. In particular, 24 h after fertilization a layer of fibrils completely covers the rhizoidal tip and connects to the substrate, functioning as a clamp to hold the germling in place (figure 3c). Similar adhesive strands are also present below the apical cell, possibly reflecting analogous chemistry and secretion mechanisms of the adhesive materials over the entire surface of the germling (figure 3d). This observation has also been made about 96 h old germlings (SEM not reported). Structurally, the fibrils extend radially in all directions, covering a considerable distance on the surface away from the germlings (5.9 ± 0.6 µm), with a length of the same order of magnitude as the width of the rhizoidal tip (8.9 ± 1.0 µm). Multiple secondary threads interconnect proximal radial strands, both sideways and vertically, cross-linking the main fibrils. Notable enlargements are present on the nodes between the primary and secondary threads, as well as on the points where the radial fibres adhere to the substrate. This characteristic helps the network to sustain loads without breakage of the internal and external connections. Small traces of adhesive materials are present further away from the main fibrous pad, similar to the adhesive remnants imaged 1 h after fertilization, suggesting a progressive expansion of the attachment pad. The overall morphology of the fibrous network resembles the structure present in spider-silk anchors, with a heterogeneous meshwork of threads rather than a homogeneous adhesive substance [67]. Such a complex structure is essential to dissipate the energy associated with detachment forces and to reduce the risk of crack propagation.

3.2. Energy dispersive X-ray spectroscopy

EDX was also performed on the rhizoidal tip of 24 h old germlings of D. antarctica (electronic supplementary material, figure S3). Traces of K, C, O, Na, S and Ca were detected in the attachment pad. The counts in the C and O peaks contain a strong contribution from the polymeric Thermanox slide used to support the specimens; therefore, quantitative conclusions on the relative amounts of the various elements identified cannot be put forth. Yet, the presence of K, Na, S and Ca is characteristic of the adhesive holdfast. Sulfated polysaccharides are abundantly present in carbohydrates secreted by large seaweed such as carrageenan and fucoidan [68], thus explaining the abundant presence of this element in the EDX spectrum. The presence of sulfated moieties will be further evidenced in §3.3. On the other hand, positive metal ions contained in seawater readily form electrostatic complexes with negatively charged polysaccharides and polyphenolics present in the adhesive secretions [46]. In particular, Ca ions mediate both adhesion and cohesion, e.g. by bridging the negatively charged adhesive secretions with a negatively charged surface and by favouring gelling of the carbohydrate network [69]. Ca ions have been extensively reported as key components in adhesive secretions in marine organisms, including bacteria [70] and sandcastle worms [71], and will probably play a key role also in brown algal attachment. The absence of a suitably developed adhesive pad in H. banksii germlings prevented EDX being used on this species to complete a full comparison between the two species. Future work will be targeted to the analysis of the footprints of the germlings to clarify the elemental composition of the adhesive pads and the putative role of the various metal ions present.

3.3. Attenuated total reflection–Fourier transform infrared analysis

3.3.1. Infrared spectra from eggs before fertilization

Prior to studying the adhesive produced by the settled germlings, preliminary IR information on the content of the peripheral structures initially present around the eggs is required. The spectra obtained from settled eggs of the two seaweed species in seawater are reported in figure 4. The absorbances are of the same order of magnitude for both spectra, confirming that a relatively uniform and densely packed monolayer of eggs was produced, also consistent with visual observation of the settled eggs on the ATR prism. Accordingly, the amount of material sampled by the IR evanescent wave will be similar for both species, and spectral differences are predominantly associated with the chemical composition of the egg surface layers. These spectra can be compared qualitatively. Note that cell size may have a minor influence on the sample of cellular material probed, but this effect was considered negligible for the experimental system investigated.

Figure 4.
ATR-FTIR spectra from settled eggs of D. antarctica (bottom line) and H. banksii (top line). Background from seawater.

Peaks in the 1000–1100 cm−1 region are in common for the two species, with a characteristic signature for polysaccharides (νC–O and ring vibrations at 1086 cm−1; νC–O–C and νC–C at 1064 cm−1; and νC–O–C and νC–C at 1035 cm−1 and νC–O–H at 1008 cm−1). From the intensity of the two spectra, it appears that the amount of polysaccharide compounds probed by the IR is higher in eggs from D. antarctica than in those from H. banksii.

Strong differences are remarkable at higher wavenumber regions. In particular, bands characteristic for phlorotannins are apparent in the spectra from H. banksii eggs, with peaks at 1610 and 1546 cm−1 (C=C aromatic ring vibrations), 1206 cm−1 (C–O–C stretch in aryl–aryl ethers) and 1157 cm−1 (C–O stretch in phenolic compounds) [72]. It is worth noting that the phlorotannin molecules probed in solution display significantly different spectra from those reported for solid phlorotannin extracts [73,74]. In particular, the bands between 1450 and 1200 cm−1, strong in the solid state, will have a dampened intensity in aqueous environment. The difference is probably owing to the formation of hydrogen bonds with water molecules, thus altering the vibrational states of the polyphenols (see the electronic supplementary material, figure S4, for spectra of phloroglucinol in the solid state versus in aqueous solution). On the other hand, D. antarctica eggs present a broad band in the 1199–1269 cm−1 range (centred at around 1240 cm−1), which is likely to be related to the An external file that holds a picture, illustration, etc.
Object name is rsif20151083-i1.jpg asymmetric stretch in complex sulfated polysaccharides such as carrageenan, porphyran and fucoidan, constituents usually abundantly present in seaweed extracts from brown algae with broad absorption in the 1195–1280 cm−1 region [30,7577]. The width of this peak could be affected by the specific configuration of the charged moiety in the polysaccharide, e.g. its position around the pyranose ring [68]. Proteins are another major constituent of the outer layer of D. antarctica eggs, with strong bands at 1651 and 1547 cm−1, related to the amide I and II modes of proteins in the α-helix conformation [78].

In conclusion, the two seaweed species share only IR signatures of carbohydrate-related compounds. In addition, while H. banksii eggs contain phlorotannin-based constituents in its periphery, the D. antarctica surface layer contains protein molecules and sulfate carbohydrates. Phlorotannins have been reported to be constituents of algal adhesives [79], but need to be cross-linked to achieve the optimal adhesive properties [46,80]. These are likely to be stored in the peripheral vesicles of H. banksii eggs, ready to be released in the extracellular environment upon fertilization [48,58]. Vesicles containing phlorotannins have also been reported for D. antarctica [81,82], but they seem to be contained within the eggs thus not probed by the IR evanescent wave [64]. Interestingly, ionic moieties present in the carbohydrate chains have a strong tendency to form stable complexes with metals and metal ions [83]. In addition, negatively charged polysaccharides are highly hygroscopic and form stable hydrogels in the presence of divalent metal ions such as Ca2+ and Mg2+ present in seawater [84]. This mechanism is consistent with the EDX results previously presented. Accordingly, the observed tendency of D. antarctica eggs to bind to substrates prior to fertilization might be explained by the presence of an adhesive layer composed of a fraction of partially secreted sulfated polysaccharides. The high protein component present in D. antarctica may be involved in the formation of the adhesive bond mediating cross-linking reactions [46,49] or simply is excreted as a result of other cellular processes.

3.3.2. Infrared spectra from Hormosira banksii germlings

Our previous microscopic observations, as well as previous studies on the formation of the adhesive mucilage [61], indicate that a connective layer of the order of a couple of micrometres thick is being produced between the germling and a substrate following fertilization. Accordingly, we hypothesize that the substances interacting with the surface, i.e. those probed by the IR beam, are the ones mostly involved in the adhesion process.

Figure 5 shows the changes in IR spectra from H. banksii germlings in the 24 h following fertilization. These results are reported as difference spectra obtained by subtracting the initial ATR-FTIR spectrum recorded at the end of the fertilization stage. Accordingly, any spectral contribution from sperm suspension is removed in the subtraction and the spectral evolution considered is representative of germling development only. The spectra are very similar to spectra of bacterial species, with distinct bands at 1651 (amide I), 1544 (amide II), 1454 (C–H deformation), 1400 (symmetric carboxylate stretch) and 1245 cm−1 (amide III, P=O and C–O–C stretch) [85]. Some spectral changes are also visible in the 1100–1000 cm−1 region characteristic of polysaccharides (C–O stretch, C–C stretch, C–O–H stretch and bend, and ring vibrations). It is important to note that these changes are only a direct consequence of the fertilization, as the spectra of the eggs before fertilization did not change with time.

Figure 5.
Band evolution from H. banksii germlings in the first 24 h after fertilization. Background is settled eggs at the end of inoculation of sperm. The temporal resolution was 20 min; lines plotted every 2 h.

PCA was carried out on the spectra of H. banksii germlings to identify the major spectral changes in the first 24 h after fertilization. The first principal component (PC1) shows a trend of sample evolution over time with early time points in negative PC1 space through to later time points in positive PC1 space (figure 6a). This principal component is describing 97% of the spectral variance in the sample and is the most important for looking at changes in the spectra. The spectral features associated with this separation are shown in the loadings plots (figure 6b) with positive features being more dominant in samples plotting in positive PC1 space and negative features being more dominant in samples plotting in negative PC1 space. The negative PC1 features at 1005, 1041 and 1205 cm−1 are stronger features in the spectra collected from earlier time points and are consistent with the polysaccharides present on the egg membrane and polyphenolics [86], respectively. The positive PC1 features at 1549 and 1655 cm−1 are more dominant in the spectra collected from later time points and are consistent with amide II and amide I features for proteins, respectively, which are released during germling development.

Figure 6.
PCA of the IR spectra collected during the development of H. banksii germlings. Pale samples represent earlier time points and darker represent later time points. (a) The scores plot represents 98% of spectral variance. (b) The loadings plots describe ...

The second principal component (PC2) only describes 1% of the spectral variance across the sample set and may not give valuable information based on chemical differences but rather the strength of the signal and baseline shape so should not be used for interpretation.

The features identified by PCA are further inspected with univariate analysis methods to look at the kinetic profiles in comparison with PC1. In particular, the kinetic development of the release of the adhesive was quantitatively tracked by measuring the relative peak absorbance of bands for key adhesive constituents, i.e. amide modes (1651 and 1544 cm−1), CH deformation (1454 cm−1) and An external file that holds a picture, illustration, etc.
Object name is rsif20151083-i2.jpg (1245 cm−1). The absorbance maxima were normalized to 1 to facilitate the comparison with PC1 (figure 7). All bands follow the same trend as the evolution of PC1, indicating that adhesion of H. banksii germlings follows a relatively simple process where all components are released with the same rate. This result also confirms that the univariate and multivariate approaches employed result in the same observations. Interestingly, the trends follow a sigmoidal shape, with a slow release of adhesive components in the first few hours, followed by a rapid increase between approximately 5 and 15 h post fertilization, and concluded by a final slow release reaching a plateau at the end of the period probed. Adhesion experiments performed by Taylor et al. [65] and Dimartino et al. [21] on H. banksii zygotes demonstrated that little to no adhesion was achieved in the first 6 h after fertilization, whereas higher adhesion strength is attained after approximately 12 h post fertilization. Our FTIR results corroborate these findings from a chemical perspective. In fact, out of the total adhesive components released in the first 24 h, only 20% are secreted in the first 6 h, whereas more than 50–60% are released at approximately 12 h after fertilization.

Figure 7.
Band kinetics of developing germlings of H. banksii normalized to 1. (Online version in colour.)

In general, the production of proteinaceous material is prevalent over other biological constituents, with bands at around 1650 and 1550 cm−1 typical of protein in the α-helix conformation. Protein-based adhesives are often reported as key components in the adhesive secretions of a number of underwater adhesive organisms such as mussels [87], barnacles [88] or arthropods [6]. The increase of the 1245 cm−1 band is mainly related to amide III absorption, with a possible contribution from post-translational modified amino acids with sulfated and phosphorylated moieties. Similar post-translational modifications are in fact typical of marine adhesive proteins [89,90].

Finally, after around 18 h, the system seems to have achieved a relatively stable steady state, with a considerably slower production of all adhesive materials.

3.3.3. Infrared spectra from Durvillaea antarctica germlings

Figure 8 presents the spectral evolution of D. antarctica germlings in the 24 h following fertilization. As observed in H. banksii germlings, the spectra for D. antarctica also present features typical of biological species, with distinct bands at 1651 (amide I), 1544 (amide II), 1454 (C–H deformation), 1400 (symmetric carboxylate stretch) and 1245 cm−1 (amide III, P=O and C–O–C stretch). In this case, the spectral changes in the 1100–1000 cm−1 region, characteristic of polysaccharides (C–O stretch, C–C stretch, C–O–H stretch and bend, and ring vibrations), are much more apparent.

Figure 8.
Band evolution in the first 24 h of D. antarctica germlings following fertilization with sperm. Background is settled eggs at the end of inoculation of sperm.

PCA was carried out on the spectra from D. antarctica germlings to identify the major spectral changes occurring in the first 24 h after fertilization. As with the PCA of H. banksii, PC1 shows a trend of sample evolution over time with early time points in negative PC1 space through to later time points in positive PC1 space (figure 9a). This principal component describes 84% of the spectral variance in the sample and is the most important for looking at changes in the spectra. The spectral features associated with this separation are shown in the loadings plots (figure 9b). The negative PC1 features at 999, 1032, 1069 (broad, shoulder) and a broad envelope between 1170 and 1290 cm−1 are stronger features in the spectra collected from earlier time points. These bands are associated with vibrational modes of polysaccharides and sulfated compounds present on the eggs and prominent during the early stages after fertilization. The positive PC1 features at 1541, 1558 and 1653 cm−1 are more dominant in the spectra collected from later time points and are consistent with amide II and amide I features, respectively, indicative of protein-based secretions in the adhesive.

Figure 9.
PCA of the IR spectra collected during the development of D. antarctica germlings. Pale samples represent earlier time points and darker represent later time points. (a) The scores plot represents 94% of spectral variance. (b) The loadings plots describe ...

PC2 accounts for a further 10% of explained spectral variance and separates early-stage zygote growth (negative PC2) from the middle time points (positive PC2), with later time points in neutral PC2 space. The spectral features associated with separation in positive PC2 space include features at 1015, 1051, 1094 (polysaccharides), 1225 (An external file that holds a picture, illustration, etc.
Object name is rsif20151083-i3.jpg), 1250 (amide III), 1360 and 1653 (amide I) cm−1. The negative separating features are observed at 988, 1072, 1119 and 1184 cm−1. It is clear from the loadings plot that baseline variation is also described by this principal component. PC2 shows temporal behaviour that suggests an increase or change in band intensities in the amide III and polysaccharide region. At later times, these changes are less distinct with evidence of band broadening particularly in the polysaccharide region. This latter finding is not inconsistent with cross-linking, which creates a variety of environments (and thus wavenumbers) for these vibrational modes.

The normalized kinetics of PC1 together with the main bands for the adhesive secretions from D. antarctica are reported in figure 10. The different trends followed by the kinetic profiles also suggest that D. antarctica produces adhesive components in a stepwise fashion. In particular, carbohydrate-related molecules with absorption in the 1000–1100 cm−1 range are released first and at a relatively higher rate. In addition, with respect to what we observed with H. banksii, in this case the area of the 1000–1100 cm−1 envelope at the end of the 24 h period is approximately 10-fold higher, indicating that D. antarctica secretes a much larger amount of polysaccharides. The band at 1245 cm−1 develops with similar kinetics to that of the carbohydrate envelope, indicating that this band is directly related to the carbohydrate secretions and, therefore, is assigned to sulfated moieties in polysaccharides. Protein-based compounds with an α-helix configuration (typical peaks for amide I and II at 1645 and 1546 cm−1) are released in the second phase of the adhesion process. The amount of protein material secreted at the end of the 24 h absorbs with similar intensity to that measured on H. banksii germlings. The kinetic evolution of the protein-related bands does not seem to be correlated to other developing bands, albeit some minor peaks are likely to be masked by the main spectral bands.

Figure 10.
Band kinetics of developing germlings of D. antarctica normalized to 1. (Online version in colour.)

Accordingly, D. antarctica germlings initially secrete negatively charged carbohydrates as the primary adhesive, and do so in much larger absolute quantities than H. banksii germlings. Protein-related constituents are released at later stages, as secondary adhesive and possibly with cross-linking functions of the previously deposited carbohydrate network. This mechanism is consistent with the microscopy results and the release of two different surface vesicles with time. Proteins have been associated with the cross-linking of carbohydrates in the cell wall of higher plants [91] and algae [92], usually through esterification reactions between uronic acid residues and hydroxyl groups on a neighbouring polysaccharide chain, but also through other more complex mechanisms [93]. The specific chemistry of the polysaccharides involved in the adhesion process, and in particular the abundance of side groups such as carboxyl or sulfate, opens up the opportunity for a new variety of ester-based cross-linking modes in the adhesive hydrogel. Interestingly, all bands display a slight reduction in the production rate at the end of the 24 h period monitored, but components were still abundantly secreted in the external environment and interacting with the prism surface, overall indicating a more copious and longer release of adhesive components in D. antarctica than previously observed in H. banksii.

3.3.4. Comparison of infrared spectra from germlings of both species

The spectra from both H. banksii and D. antarctica germlings were analysed with PCA simultaneously to find the major spectral and hence chemical differences between these two species (figure 11). Like the individual PCAs, the PC1 is the most important to describe the spectral variation of the samples over time (70% of spectral variance), with earlier spectra in negative PC1 space and later time points in positive PC1 space. The negative features are observed at 1001 (ν(C–O–H)), 1034, 1042 (ν(C–O–C) and ν(CC)), 1063 (ν(C–O–C) and ν(CC)), 1111 and a broad feature of approximately 1202 cm−1 (ν(C–O–C) aryl–aryl ether), which can be collectively attributed to polysaccharide content of the egg/zygote before secretion of the mucoadhesives. The positively separating features are observed at 1545 (broad) and 1653 cm−1, which are attributed to amide II and amide I, respectively, and believed to be the major component of the adhesive secretions. PC2 accounts for a further 24% of the spectral variance, and is paramount to highlight small variations in the spectra from these two species. This principal component separates D. antartica in positive PC2 space and H. banskii in negative PC2 space. The positive PC2 features, associated with D. antarctica, were observed at 1001, 1030, 1107 and 1246 (broad) cm−1, and are characteristic for sulfated polysaccharides. The main negative features, associated with H. banskii, were observed at 1200, 1474 and 1618 cm−1, and are indicative of polyphenolic molecules. The variance in the amide I region is difficult to interpret owing to interference from water signals.

Figure 11.
PCA of IR spectra collected during the development of D. antarctica and H. banksii germlings. Pale samples represent earlier time points and darker represent later time points. (a) The scores plot represents 94% of spectral variance. (b) The loadings ...

PCA suggests that the production of adhesive materials follows similar stages in both H. banksii and D. antarctica, with charged (mostly sulfated) polysaccharides initially present in the egg surface followed by a prominent production of proteins. This process indicates that the carbohydrate molecules are responsible for creating quick adhesive and cohesive bonds, followed by slower formation of stronger and more durable bonds through the protein secretions. The sulfated polysaccharides must be flexible and able to penetrate the layer of biofouling molecules (mostly uronic acids) ubiquitously present in marine environments [94]. The sulfated polysaccharides could be related to adhesion to the substrate through adsorption and formation of stable interactions with the surface. Because of the high concentration of divalent metal ions in seawater, and as supported by the EDX results, it is reasonable to believe that the polysaccharide matrix initially bonds through cationic bridges which can induce gelation.

Still, significant differences are found in the production mechanisms of the adhesive molecules. For example, carbohydrates are produced in H. banksii at a slower rate than in D. antarctica. But the most striking difference is the amount of material produced, especially with regards to the polysaccharide constituents, which are 10 times more prominent in D. antarctica than in H. banksii. This observation is in line with the microscopy results, where the more rapid development of D. antarctica germlings is plausibly associated with a larger release of adhesive components. Accordingly, it is reasonable to state that the adhesive fibrils observed in the SEM are mostly composed of cross-linked non-soluble carbohydrates. On the other hand, the protein component may have an important role in the direct adhesion with the surface, as well as in the formation of cohesive bonds.

4. Conclusions

In this work, the adhesive secretion from germlings of D. antarctica and H. banksii has been investigated through microscopy and spectroscopy methods. It is worth noticing that the adhesive secretions were investigated in situ, in vivo and in real time, thus offering novel and interesting insights into bioadhesives as well as confirming the strong capabilities of the experimental methods employed. Overall, the two species display many similarities in the release mechanism and in the main biological constituents of the bioadhesive. Significant differences have also been highlighted, offering important clues for the understanding of the superior attachment performance of the adhesive from D. antarctica with respect to that from H. banksii.

In general, the adhesive components are initially stored within vesicles present in the outer surface of the eggs. Following fertilization, the germlings produce a secondary mucilaginous adhesive through the release of new morphologically different vesicles. These are particularly abundant in D. antarctica but are not detected in H. banksii, probably because of a slower and less copious release. The adhesive pad has a characteristic fibrillar structure, composed of a heterogeneous meshwork of threads paramount to dissipate hydrodynamic and other detachment forces as well as minimize crack propagation. This structure is particularly complex and well established in D. antarctica germlings even after a few hours after fertilization, whereas it starts appearing in H. banksii only after at least 24 h. The difference in the amount and rate of the adhesive released is further confirmed by our ATR-FTIR results, and partly explains the improved adhesion of germlings from D. antarctica over those from H. banksii.

From a chemical standpoint, PCA reveals that the key compounds shared in the adhesive from the two species are polysaccharides, present in the primary egg vesicles and initially released, and proteins, secreted at later stages during germling development. However, the IR spectra of D. antarctica germlings indicate that their carbohydrates bear sulfated moieties, thought to mediate strong adhesive properties and cohesive characteristics. In addition, PCA suggests that cross-linking reactions, possibly facilitated by the proteins later secreted, cure the carbohydrate network, thus furthering the strength of the adhesive from D. antarctica. On the other hand, the IR spectra of H. banksii adhesive is specifically characterized by distinctive features for phlorotannins, a putative seaweed adhesive, but no other clues of rapid cross-linking reactions or other adhesive constituents are apparent.

Complex polysaccharides are abundant in seaweed and fulfil many of their biological and ecological functions [15]; therefore, it is reasonable that carbohydrate species may have a strong role also in seaweed attachment. The particular role that sulfated polysaccharides may have in the adhesion process is consistent with the formation of stable interactions with a surface and within the carbohydrate network through electrostatic and metal complex bridges.

Currently, the prominent opinion for seaweed-based adhesives describes the proteins later secreted as enzymes mediating cross-linking of the polyphenolic molecules. However, our IR results indicate that polyphenols, often invoked in the adhesion of seaweed species [47,95], are not of primary importance in the adhesion processes, and are more likely to be involved in other physiological processes. Levi & Friedlander [36] have instead proposed that protein constituents have a similar structure to vitronectin, one of the glycoproteins responsible for cell–cell bonding in other adhesion systems. In this work, we speculate that specific enzymes cross-linking the carbohydrate network are also produced, leading to the cohesive strength required to secure the germlings to the substrate and fostering the performance of the adhesive.

This work helped to clarify the mechanism of release and the key biochemical features of the strong adhesives produced by kelps. The results presented will help the design and synthesis of biomimetic counterparts, with application in wet and complex environments such as in biomedicine. In the future, molecular biology methods will be employed to assess the amino acid composition and sequence of the adhesive and cross-linking proteins probed, thus clarifying their role in the adhesive formulation. Further investigation into the specific functions of the polyphenolic fraction and its possible interaction with the proteins is also of relevance and will constitute material for future research. These objectives coupled with the assessment of the attachment performances of adhesives isolated from the two brown seaweeds will further support the development and ideally real-word application of permanent biomimetic adhesives.

Supplementary Material

Supporting Information:

Acknowledgements

We acknowledge the Department of Botany at UO and the research group of Dr Catriona Hurd (now at the University of Tasmania) for providing seawater as well as the use of the temperature-controlled growth chambers and the optical microscope; Liz Girvan in the Department of Anatomy at UO for her support with the SEM; the Marine Ecology Research Group at UC for their help with the release of seaweed gametes; Dr Luigi Petrone for initial guidance on the set-up of the ATR-FTIR; BIC at UC for funding. D.M.S. and S.J.F.-M. acknowledge the University of Otago for doctoral scholarships.

Authors' contributions

S.D. designed and coordinated the study, carried out all experiments, participated in data analysis and wrote the manuscript; D.M.S. helped in the ATR-FTIR experiments and related data analysis; S.J.F.-M. and K.C.G. carried out the principal component analyses and helped draft the manuscript; A.J.M. helped in the data analysis and drafting the manuscript. All authors gave final approval for publication.

Competing interests

The authors have no competing interests.

Funding

S.D. was supported by a postdoctoral fellowship offered by the Biomolecular Interaction Centre (BIC) at the University of Canterbury. D.M.S. and S.J.F.-M. were supported by postgraduate scholarships from the University of Otago.

References

1. Lee H, Dellatore SM, Miller WM, Messersmith PB 2007. Mussel-inspired surface chemistry for multifunctional coatings. Science 318, 426–430. (doi:10.1126/science.1147241) [PMC free article] [PubMed]
2. Aksak B, Murphy MP, Sitti M 2008. Gecko inspired micro-fibrillar adhesives for wall climbing robots on micro/nanoscale rough surfaces. In Proc. IEEE Int. Conf. on Robotics and Automation, ICRA 2008 Pasadena, CA, 19–23 May, pp. 3058–3063. Piscataway, NJ: IEEE.
3. Haller CM, Buerzle W, Brubaker CE, Messersmith PB, Mazza E, Ochsenbein-Koelble N, Zimmermann R, Ehrbar M 2011. Mussel-mimetic tissue adhesive for fetal membrane repair: a standardized ex vivo evaluation using elastomeric membranes. Prenat. Diagn. 31, 654–660. (doi:10.1002/pd.2712) [PubMed]
4. Santos R, Aldred N, Gorb S, Flammang P 2013. Biological and biomimetic adhesives: challenges and opportunities. Cambridge, UK: Royal Society of Chemistry Publishing.
5. Bianco-Peled H, Davidovich-Pinhas M 2015. Bioadhesion and biomimetics: from nature to applications. Boca Raton, FL: CRC Press.
6. Hennebert E, Wattiez R, Waite JH, Flammang P 2012. Characterization of the protein fraction of the temporary adhesive secreted by the tube feet of the sea star Asterias rubens. Biofouling 28, 289–303. (doi:10.1080/08927014.2012.672645) [PubMed]
7. Kamino K. 2006. Barnacle underwater attachment. In Biological adhesives (eds Smith AM, Callow JA), pp. 145–166. Berlin, Germany: Springer.
8. Shao H, Bachus KN, Stewart RJ 2009. A water-borne adhesive modeled after the sandcastle glue of P. californica. Macromol. Biosci. 9, 464–471. (doi:10.1002/mabi.200800252) [PMC free article] [PubMed]
9. Santos R, Flammang P 2012. Is the adhesive material secreted by sea urchin tube feet species-specific? J. Morphol. 273, 40–48. (doi:10.1002/jmor.11004) [PubMed]
10. Hennebert E, Viville P, Lazzaroni R, Flammang P 2008. Micro- and nanostructure of the adhesive material secreted by the tube feet of the sea star Asterias rubens. J. Struct. Biol. 164, 108–118. (doi:10.1016/j.jsb.2008.06.007) [PubMed]
11. Millar A, Kraft G 1994. Catalogue of marine brown algae (Phaeophyta) of New South Wales, including Lord Howe Island, south-western Pacific. Aust. Syst. Bot. 7, 1–47. (doi:10.1071/SB9940001)
12. Stevens CL, Hurd CL, Smith MJ 2002. Field measurement of the dynamics of the bull kelp Durvillaea antarctica (Chamisso) Heriot. J. Exp. Mar. Biol. Ecol. 269, 147–171. (doi:10.1016/S0022-0981(02)00007-2)
13. Harder DL, Hurd CL, Speck T 2006. Comparison of mechanical properties of four large, wave-exposed seaweeds. Am. J. Bot. 93, 1426–1432. (doi:10.3732/ajb.93.10.1426) [PubMed]
14. Seymour RJ, Tegner MJ, Dayton PK, Parnell PE 1989. Storm wave induced mortality of giant kelp, Macrocystis pyrifera, in Southern California. Estuar. Coast. Shelf Sci. 28, 277–292. (doi:10.1016/0272-7714(89)90018-8)
15. Graham JE, Wilcox LW, Graham LE 2008. Algae, 2nd edn San Francisco, CA: Benjamin Cummings.
16. Osborn JEM. 1948. The structure and life history of Hormosira banksii (Turner) Decaisne. Trans. Proc. R. Soc. N.Z. 77, 47–71.
17. McKenzie PF, Bellgrove A 2009. Dislodgment and attachment strength of the intertidal macroalga Hormosira banksii (Fucales, Phaeophyceae). Phycologia 48, 335–343. (doi:10.2216/08-96.1)
18. Stevens CL, Taylor DI, Delaux S, Smith MJ, Schiel DR 2008. Characterisation of wave-influenced macroalgal propagule settlement. J. Mar. Syst. 74, 96–107. (doi:10.1016/j.jmarsys.2007.11.006)
19. Stevens C, Hurd CL, Smith M, Harder DL 2001. Durvillaea antarctica: the strongest kelp in the world? NIWA Water Atmosph. 9, 8–9.
20. Taylor DI, Schiel DR 2003. Wave-related mortality in zygotes of habitat-forming algae from different exposures in southern New Zealand: the importance of ‘stickability’. J. Exp. Mar. Biol. Ecol. 290, 229–245. (doi:10.1016/s0022-0981(03)00094-7)
21. Dimartino S, Mather AV, Alestra T, Nawada S, Haber M 2015. Experimental and computational analysis of a novel flow channel to assess the adhesion strength of sessile marine organisms. Interface Focus 5, 20140059 (doi:10.1098/rsfs.2014.0059) [PMC free article] [PubMed]
22. Fagerberg WR, Towle J, Dawes CJ, Böttger A 2012. Bioadhesion in Caulerpa mexicana (chlorophyta): rhizoid-substrate adhesion. J. Phycol. 48, 264–269. (doi:10.1111/j.1529-8817.2012.01113.x) [PubMed]
23. Ouriques LC, Schmidt ÉC, Bouzon ZL 2012. The mechanism of adhesion and germination in the carpospores of Porphyra spiralis var. amplifolia (Rhodophyta, Bangiales). Micron 43, 269–277. (doi:10.1016/j.micron.2011.08.012) [PubMed]
24. Apple ME, Harlin MM, Norris JH 1996. Characterization of Champia parvula (Rhodophyta) tetraspore mucilage and rhizoids with histochemical stains and FITC-labelled lectins. Phycologia 35, 245–252. (doi:10.2216/i0031-8884-35-3-245.1)
25. Moss B. 1975. Attachment of zygotes and germlings of Ascophyllum nodosum (L.) Le Jol. (Phaeophyceae, Fucales). Phycologia 14, 75–80. (doi:10.2216/i0031-8884-14-2-75.1)
26. Callow ME, Evans LV, Bolwell GP, Callow JA 1978. Fertilization in brown algae. I. SEM and other observations on Fucus serratus. J. Cell. Sci. 32, 45–54. [PubMed]
27. Callow J, Osborne M, Callow M, Baker F, Donald A 2003. Use of environmental scanning electron microscopy to image the spore adhesive of the marine alga Enteromorpha in its natural hydrated state. Colloids Surf. B Biointerfaces. 27, 315–321. (doi:10.1016/S0927-7765(02)00094-2)
28. Bråten T. 1975. Observations on mechanisms of attachment in the green alga Ulva mutabilis Føyn. Protoplasma 84, 161–173. (doi:10.1007/BF02075951) [PubMed]
29. Petrone L, Easingwood R, Barker MF, McQuillan AJ 2011. In situ ATR-IR spectroscopic and electron microscopic analyses of settlement secretions of Undaria pinnatifida kelp spores. J. R. Soc. Interface 8, 410–422. (doi:10.1098/rsif.2010.0316) [PMC free article] [PubMed]
30. Chiovitti A, Heraud P, Dugdale TM, Hodson OM, Curtain RCA, Dagastine RR, Wood BR, Wetherbee R 2008. Divalent cations stabilize the aggregation of sulfated glycoproteins in the adhesive nanofibers of the biofouling diatom Toxarium undulatum. Soft Matter 4, 811–820. (doi:10.1039/B715455K)
31. Walker GC, Sun Y, Guo S, Finlay JA, Callow ME, Callow JA 2005. Surface mechanical properties of the spore adhesive of the green alga Ulva. J. Adhes. 81, 1101–1118. (doi:10.1080/00218460500310846)
32. Callow JA, Crawford SA, Higgins MJ, Mulvaney P, Wetherbee R 2000. The application of atomic force microscopy to topographical studies and force measurements on the secreted adhesive of the green alga Enteromorpha. Planta 211, 641–647. (doi:10.1007/s004250000337) [PubMed]
33. Molino PJ, Hodson OM, Quinn JF, Wetherbee R 2006. Utilizing QCM-D to characterize the adhesive mucilage secreted by two marine diatom species in-situ and in real-time. Biomacromolecules 7, 3276–3282. (doi:10.1021/bm0605661) [PubMed]
34. Molino PJ, Hodson OM, Quinn JF, Wetherbee R 2008. The quartz crystal microbalance: a new tool for the investigation of the bioadhesion of diatoms to surfaces of differing surface energies. Langmuir 24, 6730–6737. (doi:10.1021/la800672h) [PubMed]
35. Rosenhahn A, Finlay J, Pettit M, Ward A, Wirges W, Gerhard R, Callow M, Grunze M, Callow J 2009. Zeta potential of motile spores of the green alga Ulva linza and the influence of electrostatic interactions on spore settlement and adhesion strength. Biointerphases 4, 7–11. (doi:10.1116/1.3110182) [PubMed]
36. Levi B, Friedlander M 2004. Identification of two putative adhesive polypeptides in Caulerpa prolifera rhizoids using an adhesion model system. J. Appl. Phycol. 16, 1–9. (doi:10.1023/B:JAPH.0000019034.12015.87)
37. Jordan P, Vilter H 1991. Extraction of proteins from material rich in anionic mucilages: partition and fractionation of vanadate-dependent bromoperoxidases from the brown algae Laminaria digitata and L. saccharina in aqueous polymer two-phase systems. Biochim. Biophys. Acta BBA - Gen. Subj 1073, 98–106. (doi:10.1016/0304-4165(91)90188-M) [PubMed]
38. Pettitt ME, Henry SL, Callow ME, Callow JA, Clare AS 2004. Activity of commercial enzymes on settlement and adhesion of cypris larvae of the barnacle Balanus amphitrite, spores of the green alga Ulva linza, and the diatom Navicula perminuta. Biofouling 20, 299–311. (doi:10.1080/08927010400027068) [PubMed]
39. Apple ME, Harlin MM 1995. Inhibition of tetraspore adhesion in Champia parvula (Rhodophyta). Phycologia 34, 417–423. (doi:10.2216/i0031-8884-34-5-417.1)
40. Bouzon ZL, Ouriques LC 2007. Characterization of Laurencia arbuscula spore mucilage and cell walls with stains and FITC-labelled lectins. Aquat. Bot. 86, 301–308. (doi:10.1016/j.aquabot.2006.10.010)
41. Barlow DE, Wahl KJ 2012. Optical spectroscopy of marine bioadhesive interfaces. Annu. Rev. Anal. Chem. 5, 229–251. (doi:10.1146/annurev-anchem-061010-113844) [PubMed]
42. Petrone L. 2013. Molecular surface chemistry in marine bioadhesion. Adv. Colloid Interface Sci. 195–196, 1–18. (doi:10.1016/j.cis.2013.03.006) [PubMed]
43. Burden DK, Barlow DE, Spillmann CM, Orihuela B, Rittschof D, Everett RK, Wahl KJ 2012. Barnacle Balanus amphitrite adheres by a stepwise cementing process. Langmuir 28, 13 364–13 372. (doi:10.1021/la301695m) [PubMed]
44. Parikh SJ, Chorover J 2006. ATR-FTIR spectroscopy reveals bond formation during bacterial adhesion to iron oxide. Langmuir 22, 8492–8500. (doi:10.1021/la061359p) [PubMed]
45. Gao Z, Bremer PJ, Barker MF, Tan EW, McQuillan AJ 2007. Adhesive secretions of live mussels observed in situ by attenuated total reflection infrared spectroscopy. Appl. Spectrosc. 61, 55–59. (doi:10.1366/000370207779701398) [PubMed]
46. Bitton R, Ben-Yehuda M, Davidovich M, Balazs Y, Potin P, Delage L, Colin C, Bianco-Peled H 2006. Structure of algal-born phenolic polymeric adhesives. Macromol. Biosci. 6, 737–746. (doi:10.1002/mabi.200600073) [PubMed]
47. Vreeland V, Waite JH, Epstein L 1998. Minireview—polyphenols and oxidases in substratum adhesion by marine algae and mussels. J. Phycol. 34, 1–8. (doi:10.1046/j.1529-8817.1998.340001.x)
48. Schoenwaelder MEA, Clayton MN 1998. Secretion of phenolic substances into the zygote wall and cell plate in embryos of Hormosira and Acrocarpia (Fucales, Phaeophyceae). J. Phycol. 34, 969–980. (doi:10.1046/j.1529-8817.1998.340969.x)
49. Vreeland V, Epstein L 1996. Analysis of plant-substratum adhesives. In Modern methods of plant analysis, vol. 17 (eds Linskens HF, Jackson JF), pp. 95–116. Berlin, Germany: Springer.
50. Crayton MA, Wilson E, Quatrano RS 1974. Sulfation of fucoidan in Fucus embryos: II. Separation from initiation of polar growth. Dev. Biol. 39, 164–167. (doi:10.1016/S0012-1606(74)80018-7) [PubMed]
51. Bitton R, Bianco-Peled H 2008. Novel biomimetic adhesives based on algae glue. Macromol. Biosci. 8, 393–400. (doi:10.1002/mabi.200700239) [PubMed]
52. McGoverin CM, Clark ASS, Holroyd SE, Gordon KC 2010. Raman spectroscopic quantification of milk powder constituents. Anal. Chim. Acta. 673, 26–32. (doi:10.1016/j.aca.2010.05.014) [PubMed]
53. Killeen DP, Sansom CE, Lill RE, Eason JR, Gordon KC, Perry NB 2013. Quantitative Raman spectroscopy for the analysis of carrot bioactives. J. Agric. Food Chem. 61, 2701–2708. (doi:10.1021/jf3053669) [PubMed]
54. Fraser SJ, Natarajan AK, Clark ASS, Drummond BK, Gordon KC 2015. A Raman spectroscopic study of teeth affected with molar–incisor hypomineralisation. J. Raman Spectrosc. 46, 202–210. (doi:10.1002/jrs.4635)
55. Smith GPS, McGoverin CM, Fraser SJ, Gordon KC 2015. Raman imaging of drug delivery systems. Adv. Drug Deliv. Rev. 89, 21–41. (doi:10.1016/j.addr.2015.01.005) [PubMed]
56. Dimartino S, Savory DM, McQuillan AJ 2014. Preparation of biological samples for the study of wet-resistant adhesives inspired by kelps. In Proc. the 2014 Chemeca Conference, Perth, Australia, 28 September–1 October 2014.
57. Petrone L, Ragg NLC, McQuillan AJ 2008. In situ infrared spectroscopic investigation of Perna canaliculus mussel larvae primary settlement. Biofouling 24, 405–413. (doi:10.1080/08927010802339970) [PubMed]
58. Kevekordes K, Clayton MN 2000. Development of Hormosira banksii (Phaeophyceae) embryos in selected components of secondarily-treated sewage effluent. J. Phycol. 36, 25–32. (doi:10.1046/j.1529-8817.2000.99059.x)
59. Kevekordes K, Clayton M 1999. Shedding of the zygote wall by Durvillaea potatorum (Durvillaeales, Phaeophyta) embryos. Eur. J. Phycol. 34, 65–70. (doi:10.1080/09670269910001736092)
60. Boulbene B, Morchain J, Bonin MM, Janel S, Lafont F, Schmitz P 2012. A combined computational fluid dynamics (CFD) and experimental approach to quantify the adhesion force of bacterial cells attached to a plane surface. AIChE J. 58, 3614–3624. (doi:10.1002/aic.13747)
61. Forbes MA, Hallam ND 1979. Embryogenesis and substratum adhesion in the brown alga Hormosira banksii (Turner) Decaisne. Br. Phycol. J. 14, 69–81. (doi:10.1080/00071617900650101)
62. Forbes MA, Hallam ND 1978. Gamete structure and fertilization in the brown alga Hormosira banksii (Turner) Decaisne. Br. Phycol. J. 13, 299–310. (doi:10.1080/00071617800650361)
63. Evans LV, Callow JA, Callow ME 1982. The biology and biochemistry of reproduction and early development in Fucus. Prog. Phycol. Res. 1, 67–110.
64. Schoenwaelder MEA. 2002. The occurrence and cellular significance of physodes in brown algae. Phycologia 41, 125–139. (doi:10.2016/i0031-8884-41-2-125.1)
65. Taylor D, Delaux S, Stevens C, Nokes R, Schiel D 2010. Settlement rates of macroalgal algal propagules: cross-species comparisons in a turbulent environment. Limnol. Oceanogr. 55, 66–76. (doi:10.4319/lo.2010.55.1.0066)
66. Scardino AJ, Guenther J, de Nys R 2008. Attachment point theory revisited: the fouling response to a microtextured matrix. Biofouling 24, 45–53. (doi:10.1080/08927010701784391) [PubMed]
67. Wolff JO, Grawe I, Wirth M, Karstedt A, Gorb SN 2015. Spider's super-glue: thread anchors are composite adhesives with synergistic hierarchical organization. Soft Matter 11, 2394–2403. (doi:10.1039/C4SM02130D) [PubMed]
68. Pereira L, Gheda SF, Ribeiro-Claro PJA 2013. Analysis by vibrational spectroscopy of seaweed polysaccharides with potential use in food, pharmaceutical, and cosmetic industries. Int. J. Carbohydr. Chem. 2013, 1–7. (doi:10.1155/2013/537202)
69. Petrone L, Ragg NLC, Girvan L, McQuillan AJ 2009. Scanning electron microscopy and energy dispersive X-ray microanalysis of Perna canaliculus mussel larvae adhesive secretion. J. Adhes. 85, 78–96. (doi:10.1080/00218460902782055)
70. Marshall KC, Stout R, Mitchell R 1971. Mechanism of the initial events in the sorption of marine bacteria to surfaces. J. Gen. Microbiol. 68, 337–348. (doi:10.1099/00221287-68-3-337)
71. Sun CJ, Fantner GE, Adams J, Hansma PK, Waite JH 2007. The role of calcium and magnesium in the concrete tubes of the sandcastle worm. J. Exp. Biol. 210, 1481–1488. (doi:10.1242/jeb.02759) [PubMed]
72. Socrates G. 2004. Infrared and Raman characteristic group frequencies: tables and charts, 3rd edn Chichester, UK: Wiley.
73. Ragan MA. 1985. The high molecular weight polyphloroglucinols of the marine brown alga Fucus vesiculosus L.: degradative analysis. Can. J. Chem. 63, 294–303. (doi:10.1139/v85-050)
74. Ragan MA, Glombitza KW 1986. Phlorotannins, brown algal polyphenols. Prog. Phycol. Res. 4, 129–241.
75. Anno K, Seno N, Ota M 1970. Isolation of l-fucose 4-sulfate from fucoidan. Carbohydr. Res. 13, 167–169. (doi:10.1016/S0008-6215(00)84905-8)
76. Li B, Lu F, Wei X, Zhao R 2008. Fucoidan: structure and bioactivity. Molecules 13, 1671–1695. (doi:10.3390/molecules13081671) [PubMed]
77. Cael JJ, Isaac DH, Blackwell J, Koenig JL, Atkins EDT, Sheehan JK 1976. Polarized infrared spectra of crystalline glycosaminoglycans. Carbohydr. Res. 50, 169–179. (doi:10.1016/S0008-6215(00)83848-3) [PubMed]
78. Barth A. 2007. Infrared spectroscopy of proteins. Biochim. Biophys. Acta BBA - Bioenerg. 1767, 1073–1101. (doi:10.1016/j.bbabio.2007.06.004) [PubMed]
79. Vreeland V, Grotkopp E, Espinosa S, Quiroz D, Laetsch WM, West J 1993. The pattern of cell wall adhesive formation by Fucus zygotes. Hydrobiologia 260–261, 485–491. (doi:10.1007/BF00049060)
80. Grotkopp E, , Vreeland V.1996. Aqueous algal-based phenolic type adhesives and glues. US Patent no. 5520727.
81. Schoenwaelder MEA, Clayton MN 1999. The presence of phenolic compounds in isolated cell walls of brown algae. Phycologia 38, 161–166. (doi:10.2216/i0031-8884-38-3-161.1)
82. Clayton MN, Ashburner CM 1994. Secretion of phenolic bodies following fertilisation in Durvillaea potatorum (Durvillaeales, Phaeophyta). Eur. J. Phycol. 29, 1–9. (doi:10.1080/09670269400650411)
83. Stumm W, Kummert R, Sigg L 1980. A ligand exchange model for the adsorption of inorganic and organic ligands at hydrous oxide interfaces. Croat. Chem. Acta. 53, 291–312.
84. Adair WS. 2012. Organization and assembly of plant and animal extracellular matrix. San Diego, CA: Academic Press.
85. Kang S-Y, Bremer PJ, Kim K-W, McQuillan AJ 2006. Monitoring metal ion binding in single-layer Pseudomonas aeruginosa biofilms using ATR–IR spectroscopy. Langmuir 22, 286–291. (doi:10.1021/la051660q) [PubMed]
86. Gattrell M, Kirk DW 1992. A Fourier transform infrared spectroscopy study of the passive film produced during aqueous acidic phenol electro-oxidation. J. Electrochem. Soc. 139, 2736–2744. (doi:10.1149/1.2068972)
87. Silverman HG, Roberto FF 2007. Understanding marine mussel adhesion. Mar. Biotechnol. 9, 661–681. (doi:10.1007/s10126-007-9053-x) [PMC free article] [PubMed]
88. Kamino K. 2008. Underwater adhesive of marine organisms as the vital link between biological science and material science. Mar. Biotechnol. 10, 111–121. (doi:10.1007/s10126-007-9076-3) [PubMed]
89. Waite JH, Tanzer ML 1981. Polyphenolic substance of Mytilus edulis: novel adhesive containing l-dopa and hydroxyproline. Science 212, 1038–1040. (doi:10.1126/science.212.4498.1038) [PubMed]
90. Taylor SW, Waite JH, Ross MM, Shabanowitz J, Hunt DF 1994. trans-2,3-cis-3,4-dihydroxyproline, a new naturally occurring amino acid, is the sixth residue in the tandemly repeated consensus decapeptides of an adhesive protein from Mytilus edulis. J. Am. Chem. Soc. 116, 10 803–10 804. (doi:10.1021/ja00102a063)
91. Brown JA, Fry SC 1993. Novel O-d-galacturonyl esters in the pectic polysaccharides of suspension-cultured plant cells. Plant Physiol. 103, 993–999. (doi:10.1104/pp.103.3.993) [PubMed]
92. Waffenschmidt S, Woessner JP, Beer K, Goodenough IJW 1993. Isodityrosine cross-linking mediates insolubilization of ceIl walls in Chlamydomonas. Plant Cell 5, 809–820. (doi:10.1105/tpc.5.7.809) [PubMed]
93. Iiyama K, Lam TBT, Stone BA 1994. Covalent cross-links in the cell wall. Plant Physiol. 104, 315–320. (doi:10.1104/pp.104.2.315) [PubMed]
94. Stewart RJ, Ransom TC, Hlady V 2011. Natural underwater adhesives. J. Polym. Sci. B, Polym. Phys. 49, 757–771. (doi:10.1002/polb.22256) [PMC free article] [PubMed]
95. Berglin M, Delage L, Potin P, Vilter H, Elwing H 2001. Enzymatic cross-linking of a phenolic polymer extracted from the marine alga Fucus serratus. Biomacromolecules 5, 2376–2383. (doi:10.1021/bm0496864) [PubMed]

Articles from Journal of the Royal Society Interface are provided here courtesy of The Royal Society