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Curr Opin Chem Biol. Author manuscript; available in PMC 2017 April 1.
Published in final edited form as:
PMCID: PMC4870124
NIHMSID: NIHMS754584

Biocatalytic Portfolio of Basidiomycota

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Introduction

Basidiomycota and Ascomycota represent the major phyla of the fungal kingdom which is estimated to be comprised of more than 1.5 mio species [1]. Only a fraction (~100,000 species) of this diversity has been described and less than a 10th of the known species have been characterized in more detail and/or exploited by humans. Ascomycota (~70% of the described species) have so far received the greatest attention by scientists and for applications in biotechnology while Basidiomycota remain greatly understudied despite their importance for carbon recycling, ecosystem functioning and medicinal properties. The Basidiomycota are distinguished from the Ascomycota by the formation of cellular structures known as basidia on which haploid, sexual spores (basidiospores) are produced. Unlike Ascomycota, most Basidiomycota have long-lived dikaryotic states and their hyphae grow and divide as dikaryons with two genetically different nuclei. Mitotic division of growing hyphal cells involves passage of one nucleus back over a developing septum between parent and daughter cell via a readily observed clamp structure. Many Basidiomycota produce characteristic fruiting bodies with club-like spore-bearing basidia that line gills, pores or surfaces (e.g. gilled mushrooms, boletes, chanterelles, polypores, coral fungi) or are sometimes located inside of fruiting bodies (e.g. puffballs). Other Basidiomycota classes with different basidia and fruiting body structures include the jelly, smut and rust fungi.

Funding of bioenergy and biofuel research in the past decade, however, has greatly accelerated research aimed at characterizing the lignocellulolytic mechanisms and enzymes of Basidiomycota. Through efforts of the US Department of Energy’s (DOE) Fungal Genome Program, resources and information are being built as a foundation to tap into the unique biochemical abilities of these fungi for biotechnological applications. The DOE’s Joint Genome Institute’s (JGI) MycoCosm Portal (http://jgi.doe.gov/fungi) [2] now lists close to 200 genome sequences of Basidiomycota compared to fewer than 10 available genomes only a few years ago. The majority of genomes for basidiomycete fungi are of wood- [39] and leaf litter-decaying [10] mushroom-forming fungi (Agaricomycetes), including a few medicinally relevant fungi [6,1113]. More recently, mushrooms with different nutritional modes such as mycorrhizal [14] and plant pathogenic fungi [15] have been added to the list of genera with sequenced genomes.

The steady influx of genome data has facilitated detailed comparative genomic studies aimed at understanding the evolution and function of fungal lignocellulose degradation [3,5,9,10]. These studies have uncovered a wealth of known and novel oxidoreductase and hydrolase families of which many have undergone lineage specific expansions and reductions to support specialized wood decay mechanisms and lifestyles. Although relatively few members of these families have been functionally studied (reviewed in: [1619]), the characterized enzymes point to an intricately interwoven tapestry of lignocellulolytic activities that are useful for applications beyond lignocellulose breakdown, including future efforts at finding novel biotransformation activities. Little attention has so far been paid to enzymes involved in the biosynthesis of the diverse secondary metabolites made by Basidiomycota. Genome mining efforts [6,1113,20] and the numerous natural products [21] isolated from cultures or fruiting bodies suggest a treasure trove of novel catalytic activities awaiting to be discovered.

This review will provide an overview of the biocatalytic portfolio of Basidiomycota characterized to date with the hope of motivation more research into the chemical toolkits of this diverse group of fungi. Emphasis will be placed on enzymatic activities useful for bioctalysis and biosynthesis. To appreciate the yet largely uncharacterized, biocatalytic abilities of Basidiomycota, it is necessary to understand their diverse lifestyles and ecological functions which have shaped their secondary metabolomes and enzyme repertoires.

Fungal life styles at a glance

Basidiomycota can roughly be divided into saprobic, symbiontic or parasitic/pathogenic groups based on their nutritional modes. Saprobic fungi decompose organic matter (wood, leaf litter or dung) while symbiotic fungi form a mutualistic relationship with another organism such for example plants where >80% of species engage in such symbiotic relationships. For example, ectomycorrhizal fungi exchange nutrients with trees and shrubs via mycorrhiza that envelop root tips to provide phosphorous, nitrogen and other nutrients to the plant in exchange for photosynthetic sugars. These fungi therefore play a critical role in the function of forest ecosystems. Pathogenic or parasitic fungi infect plants, animals and even other fungi.

Following the evolution of lignin biosynthesis in land plants, the ancestors of basidiomycete fungi evolved ~ 295 million years ago the ability to completely mineralize lignocellulose [5], thereby bringing an end to the carboniferous period by releasing CO2 from plant biomass back into the atmosphere. These white-rot (WR) fungi evolved high-redox potential class II peroxidases able to directly attack lignin to oxidize and break non-aromatic bonds of this complex and recalcitrant polymer. This family of class II peroxidases (PODs) was subsequently expanded, but also lost again in most brown-rot (BR) fungi and are absent or reduced in mycorrhizal fungi. Only WR fungi are able to simultaneously depolymerize and mineralize polysaccharide and lignin-polymers to a white mass, while BR fungi only modify lignin in order to access and aggressively degrade the polysaccharide polymers of lignocellulose, leaving a brownish discolored and brittle wood residue behind. On a genome levels, these two types of wood-decay fungi can typically be distinguished by the presence or absence of lignocellulose depolymerizing enzymes; only WR fungi have lignin degrading PODs as well as GH6 and GH7 family cellobiohydrolases [3,5]. Mycorrhizal life styles have evolved from different WR and BR fungi leading to a reduction of lineage specific lignocellulolytic enzyme repertoires as carbohydrates are derived from plant and the development of new symbiosis specific protein repertoires [14]. Non-woody litter degrading fungi have a reduced ability to degrade lignin, but have evolved instead a generalist approach to utilize diverse carbohydrates and deal with a variety of aromatic compounds present in litter and compost by expanding e.g. their heme-thiolate peroxygenase repertoires [10].

Fungal mycelial networks are continuously remodeled to access new nutrient sources. Many saprotrophic fungi scout for new nutrient sources by sending out long-range linear hyphal organs (cords) that can lead to the formation of extensive networks connecting multiple nutritient sources. Basidiomycota also form fruiting bodies for spore dispersal to establish new mycelial networks. Underground hyphae and fruiting bodies are vulnerable to a number of threats, including antagonistic and competing fungi and bacteria as well as grazing by invertebrates such as small arthropods, insects, nematodes, mollusks and larger animals [22]. In response, fungi have developed diverse defense mechanisms such as the production of secondary metabolites as defense and/or microbial and fauna control agents [2325]. Hyphae invading wood or decomposing litter also encounter a range of potentially toxic lignin degradation products and plant defense compounds, including mono- and polyphenolic compounds and resins with high contents of terpenes. Consequently, Basidiomycota have evolved distinct secondary metabolite profiles and biochemical arsenals.

Masters of redox biocatalytic transformations

Wood- or litter decomposing fungi secrete suites of oxidoreductases to modify and breakdown lignocellulolytic polymers and/or deal with toxic compounds (Box 1 and Figure 1) [17,26]. Hundreds of cytochrome P450 monooxygenase (P450s) homologs are typically present in their genomes [27] along with large numbers flavin-dependent oxidoreductases. Many of these enzymes are found in secondary metabolic clusters [13,28], others, especially P450s, are assumed to be part of the fungal defense system against xenobiotics [29].

Figure 1
Oxidative biotransformations involved in lignin degradation

Box 1: Summary of major classes of oxidoreductases in Basidiomycota

Enzymes are grouped into five classes (gray boxes) based on their reactions. EC numbers and auxillary activity (AA) family numbers if in the carbohydrate-enzymes database CaZy (www.cazy.org).

Peroxidases EC.1.11.X, AA2Single electron oxidation without oxygen transfer: [S]H2 +H2O2 [left and right double arrow ] [P] + 2H2O

Class II heme peroxidases (PODs) EC 1.11.1Secreted by lignin depolymerizing WR fungi; enzymes share a conserved helical fold with 2 domains forming a heme-binding cleft.
Lignin Peroxidase (LiP) EC 1.11.1.14High redox potential (RP) (1.4–1.5 V) of LiP facilitates oxidation of non-aromatic compounds without a mediator; LiP exhibit broad substrate specificity with (non)aromatic compounds and catalyze cleavage of the Cα-Cβ linkages of lignin polymers; Substrate oxidation occurs via long-range electron transfer to the heme from a hydroxylated Trp residue located on the protein surface.
Manganese Peroxidase (MnP) EC 1.11.1.13MnP (RP: 1.0–1.2 V) transfer one electron to Mn2+ to generate Mn3+ which upon chelation by carboxylic acids (e.g. oxalates) becomes a diffusible oxidant that abstracts electrons from aromatic compounds to generate radicals.
Versatile Peroxidase (VP) EC 1.11.1.16,VP (RP: 1.4–1.5 V) exhibit broad substrate specificity and combine activities of both LiP and MnP such as the high-redox potential of LiP and the Mn2+ binding pocket of MnP.

Dye-decolorizing Peroxidases (DyP) EC 1.11.1.19DyP (RP: 1.2–1.5 V) are secreted peroxidases that can oxidize a broad range of aromatic compounds and compounds with conjugated double bonds; DyP are particularly known for their oxidation of various recalcitrant anthraquinone dyes; DyP natural substrates and functions are not known but they may play a role in lignin degradation despite having a lower redox potential than LiP. The structural fold of DyPs is distinct from other peroxidases as their heme binding pocket is located in a C-terminal domain. DyPs are evolutionary related to chlorite dismutases; their catalytic mechanism has yet to be fully elucidated.

Heme-thiolate Peroxygenase EC 1.11.2Incorporation of one oxygen from H2O2 into substrate: [S]H + H2O2 [left and right double arrow ] [P]-OH + H2O

Unspecific Peroxygenase (UPO) EC 1.11.2.1UPOs (RP: 0.9–1.2 V) are heme-containing enzymes with a P450 spectrum. They catalyze similar reactions than P450 monooxygenases by using the P450 “peroxide shunt” pathway. UPOs oxidize many aromatic, aliphatic and heterocyclic substrates.

Chloroperoxygenase (CPO) EC 1.11.1.10CPOs are heme-containing enzymes with P450 characteristics that catalyzes one and two electron oxidations of halides, hydroxylation/epoxidation of benzylic carbons, double bonds of linear and cyclic alkanes, organic sulfides and halogenated substrates CPOs unlike UPOs do not oxidize aromatic ring carbons or alkanes.

Peroxide generating enzymesTransfer of two electrons to O2: [S] + O2 [left and right double arrow ] [S]ox+ H2O2
Generate H2O2 for i) non-enzymatic generation of diffusible oxidants via Fenton chemistry and ii) various peroxidases and peroxygenases.

Glucose-methanol-choline (GMC) oxidoreductase EC 1.1.X.X, AA3GMCs are flavin containing enzymes that catalyze the oxidation of a wide range of substrates in a first half reaction and reduce O2 to H2O2 in a second half reaction to regenerate the oxidized flavin co-factor: [S]OH +FAD + O2 [left and right double arrow ] [S]O + FAD+ H2O2
GMC are a widely distributed family of extra- and intracellular enzymes that includes the aryl-alcohol -, pyranose – and methanol oxidases which generate H2O2 for lignin depolymeration.
Aryl-Alcohol oxidase(AAO) EC 1.1.3.7AAO oxidize aromatic alcohols.
Pyranose oxidase (Pox) EC1.1.3.10Pox xatalyzes the regioselective oxidation of aldopyranoses at the C2-position to form the corresponding keto-aldoses. Located in the periplasmic space of fungal hyphae.

Copper radical oxidase (CRO) EC 1.1.3, AA5Reaction: [S]O + 2O2 [left and right double arrow ] [S]OOH + H2O2
CROs fall into several subfamilies that are widely distributed in Basidiomycota. Functions of most subfamilies uncharacterized
Glyoxyl oxidase (GLX) EC 1.1.3.1GLC oxidize simple aldehydes to hydroxyl-carbonyls to generate small organic acids as Mn3+ chelators. Their active site is similar to those of galactose oxidase

Multi-copper oxidase EC 1.10.3.X, AA1Catalyze 4 electron reduction of O2 to H2O: 4 [S]OH + O2 [left and right double arrow ] 4[S]O + 2H2O

Laccase (LAC) EC 1.10.3.2LAC contain 4 copper ions of three different types; three copper ions form a trinuclear cluster LACs have moderate redox potentials (RP: 0.4–0.8 V) allowing them to abstract electrons from phenolic compounds to generate phenoxy radicals. LACs catalyze the oxidation of non-phenolic compounds in the presence of mediators

Benzoquinone reductase (BQR) EC 1.6.5.6, AA6FAD-containing intracellular oxidoreductase that reduces benzoquinones to the corresponding alcohols to fuel extracellular quinone redox cycling for H2O2 and radical generation: benzoquinone + NADPH + H+ [left and right double arrow ] hydroquinone+ NADP++ H2O

P450 monoxygenaseP450s incorporate oxygen from molecular oxygen into a wide range of substrates: [S] + O2 + NADPH2 [left and right double arrow ] [P]O + NADP + H2O
P450s oxidize unreactive carbons and a play major role in the detoxification of xenobiotics and secondary metabolism. P450 families have been greatly expanded in Basidiomyctoa.

DioxygenasesDioxygenases transfer two oxygens from molecular oxygen into substrates: [S] + O2 + NADPH2 [left and right double arrow ] [P]O2
Lipoxygenases (LOX) are a class of non-heme iron containing oxidoreductases that typically catalyze the regio- and stereoselective insertion of molecular oxygen into cis-polyunsaturated fatty acids to produce compounds involved in signaling and defense. LOX in Basidiomycota are responsible for the generation of oxygenated C8 volatiles that generate the typical mushroom odor.

Biocatalysts of oxidative lignin degradation

WR fungi secret three types of lignin degrading class II heme containing peroxidases: lignin (LiP), manganese (MnP) and versatile (VP) peroxidase [17]. LiP and VP have high redox potentials compared to laccases and other peroxidases that allows them to oxidize wide range of aromatic compounds and cleave the non-phenolic Cα-Cβ linkages of lignin polymers in the absence of a small molecule mediator (Figure 1). MnP catalyzes the oxidation of Mn2+ to Mn3+ which functions as diffusible oxidant upon complexation with small organic acids such as e.g. oxalic acid. VP combines both catalytic activities of LiP and MnP. Basidiomycota secrete additional oxidases that modify lignin through the generation of reactive aromatic radicals like e.g. phenoxy radicals. Dye decolorizing heme binding PODs (DyP) [18] can oxidize a range of polycyclic aromatic compounds, phenols and some lignin model compounds [3032] and may play a role both in lignin depolymerization and in the oxidation of xenobiotics. Secreted laccases also oxidize phenolic compounds and generate radicals, but also play a role in the reoxidation of hydroquinones and aryl-alcohols. The latter serves as substrate for alcohol aryl oxidase (AAO) which generates the H2O2 for peroxidases and radical generating Fenton chemistry (Figure 1). AAO belongs to the large group of catalytically versatile FAD binding GMC-type oxidoreductases [33,34] and reduces a range of aryl aclohols to the corresponding aldehydes. BR fungi rely exclusively on an extracellular Fenton chemistry that uses Fe2+ and H2O2 to produce hydroxyl radicals to attack lignin polymers. Quinone metabolites are reduced to hydroquinones by a quinone reductase prior to secretion to drive quinone redox cycling (Figure 1) for the generation of Fe2+ and hydroxyl-, perhydroxy-radicals and H2O2 [35].

Heme thiolate peroxygenases

Basidiomycota secrete two types of multi-talented oxygenases with cytochrome P450-like spectral properties: the classic and well-studied chloroperoxygenases (CPO) and the more recently discovered unspecific peroxygenases (UPO, formerly aromatic peroxygenase APO) [36]. Both enzymes catalyze the transfer of oxygen from H2O2 into a range of substrate mechanistically akin to the inefficient peroxide shunt pathway of P450s [37,38]. Yet unlike P450s, these enzymes are secreted, highly stable and do not require a reductase partner or NADPH as co-factor, which makes them very attractive for biocatalytic applications. CPO is a well characterized and already widely used biocatalyst that has a preference for unspecific halogenation and oxygenation of activated carbons like those in aromatic rings. UPO’s, however, are capable of catalyzing a spectrum oxygenation reactions, including the oxygenation of unactivated carbons, catalyzed by P450 monooxygenases [16]. Three UPO enzymes have been characterized that catalyze many useful reactions, including the epoxidation of alkenes, styrenes [39,40], selective hydroxylation of long-chain fatty acids, alkanes and alcohols, cyclic alkanes and flavonoids [4144] (Figure 2A). UPO’s utility for biocatalysis has been demonstrated with several one-pot syntheses of pharmaceutically relevant compounds [4548] and with a multi-enzyme process for 5-hydroxymethylfurfural conversion [49]. Recent insights into UPO structure and reaction mechanism [5053] as well as optimization of heterologous secretion by yeast [54] have laid the foundation for the production of rational designed enzymes for biocatalysis. BLAST searches of fungal genomes identifies hundreds of additional UPO homologs that may be screened as potential biocatalysts.

Figure 2
Representative reactions catalyzed by versatile Basidiomycota oxygenases

Monoxygenases and dioxygenases

The genomes of Basidiomycota contain large numbers of 100 to more than 250 P450 homologs. Several catalytically versatile cytochrome P450 (CYP) families such as CYP63 and CYP5144 are enriched in most Basidiomycota and are presumed to participate in the degradation of xenobiotic compounds [27,29,55]. CYP5144 members are also frequently found in secondary metabolic gene clusters [12,55], including sesquiterpenoid gene clusters described by us [13,28,56]. Other CYP families (e.g. CYP5348, CYP5359) have been expanded in a species specific manner, probably as an adaptation to specific ecological niches. Members of several of these CYP families have been shown to oxidize diverse compounds including various hydrocarbons, plant metabolites, pharmaceuticals and steroids [12,55]. A genome-wide functional screening in S. cerevisiae was carried out with a total of 300 P450s amplified from cDNA of the brown rotter Postia placenta and white rotter Phanerochaete chrysosporium [57,58]. A significant fraction of the cloned P450s was functional in S. cerevisia and showed a wide range of activities against various model substrates, demonstrating that the Basidiomycota CYPome is a rich source for the identification of novel P450 activities. Nevertheless, although a considerable number of P450s from these two Basidiomycota were functional in S. cerevisiae, in our experience functional expression of these ER membrane-bound enzymes is still largely a hit or miss undertaking often requiring extensive optimization of expression conditions, including testing different hosts (e.g. S. cerevisiae, P. pastoris, E. coli) and/or optimization or truncation of the N-terminal transmembrane domain and co-expression of a fungal reductase partner. Even after exhaustive optimization attempts, functional expression may not be achieved using current strategies and hosts.

In addition to P450s, two lipoxygenase-type dioxygenase (LOX) have been identified in Pleurotus mushrooms [59,60]. These enzymes are considered to be responsible for the formation of oxidized octane volatiles that create the typical mushroom aroma. One of the characterized LOX enzymes was found to accept bulky terpenoids as substrates. This enzyme catalyzes the selective hydroperoxidation of several sesqui- and monterpenes, including the biotransformation of (+)-valencene into the valuable aroma compound (+)-nootkatone [60,61] (Figure 2B).

Biocatalysts of secondary metabolism

Homology-based analysis of fungal genomes for known biosynthetic genes involved in secondary metabolism together with information on isolated natural products suggests that the secondary metabolome of Basidiomycota differs from those of other prolific microbial natural products producers [20,62]. Sesquiterpene synthases appear to be the most abundant class of natural products scaffold generating enzymes (see [20] for details) in these fungi. Compared to Ascomycota that may have 20-50 PKS or NRPS genes, Basidiomycota have comparatively few (on average fewer than 15) of these genes. So far, only a handful of these synthases have been studied [6368]. Two NRPS lacking the peptide-bond forming condensation domain have been biochemically characterized and shown to condense two aromatic α-keto acids to produce compounds with a quinone core as pigments or antimicrobials [63,65]. These secondary metabolites are also secreted as iron reductants to drive quinone redox cycling for lignin degradation [69]. Nevertheless, some intriguing PKS derived bioactive metabolites have been isolated from Basidiomycota. These include the aromatic acetylenic compounds like e.g. the antrocamphins from the medicinal mushroom Antrodia camporata [11,70] and the choropropynyl bearing gymnopalynes from Gymnopus sp. [71]. Biosynthesis of these compounds likely involves either a fatty acid desaturase/acetenylase system with shorter chain length specificity like the recently characterized acetenylase from Cantharellus formosus [72], or an acyl carrier protein/acyl-CoA-ligase/desaturase system analogous to the cyanobacterial enzymes that install the terminal alkyne in jamaicamide [73].

Hundreds of terpenoids have been isolated from Basidiomycota, many with unique hydrocarbon skeletons not made by other organisms [62]. Sesquiterpene synthases (STS) catalyze the 1,11-cyclization of farnesyl diphosphate (FPP) into characteristic scaffolds that can undergo further rearrangement reactions; giving rise to additional structures (Figure 3). These scaffolds are often associated with specific fungal genera such as e.g. the lactaranes made by the milk-cap Lactarius mushrooms [74]. We have biochemically characterized twenty STS from several Basidiomycota, including the six STS genes in C. cinerea [56] and ten STS genes in the anti-cancer illudin producer Omphalotus olearius [13]. We identified two 1,11-cyclizing Δ6-protoilludene synthases located in two gene clusters that synthesize the illudin scaffold. Based on the product specificities of these enzymes, we developed a predictive framework for sesquiterpenoid pathway discovery in Basidiomycota. Phylogenetic analysis of STS homologs identified in Basidiomycota genomes resulted in five clades predicted to include STS catalyzing different initial cyclization reactions of FPP. About 30% of all STS homologs identified in Basidiomycota genomes are predicted to catalyze the 1,11-cyclization of FPP to generate the bioactive sesquiterpenoid scaffolds known from Basidiomycota. We applied this framework for the functional prediction of 18 STS in S. hirsutum which is known to produces diverse bioactive sesquiterpenoids [62]. We identified and characterized three additional protoilludene synthases located in large gene clusters [28,75]. With these functionally characterized STS sequences at hand, we noticed that automated natural products annotation programs [76] only find a fraction of the actual STS genes encoded in Basidiomycota genomes. For example, out of the 18 STS genes from S. hirsutum, only six STS are included in a recent genome analysis [3].

Figure 3
Basidiomycota produces a plethora of protoilludene derived sesquiterpenoid natural products

The 1,11-cyclizing protoilludene synthases are frequently associated with large biosynthetic gene clusters, while the large majority of the STS catalyzing other cyclization reactions typically are not [13,28]. These gene clusters usually contain several P450s and GMC oxidoreductases presumably responsible for the scaffold rearrangements and oxygenations that lead to the bioactive products (Figure 3). Interestingly, Stereum makes protoilludene-derived bis-sesquiterpenoid stereostreins via a postulated Diels-Alder condensation reaction [77]. Agrocybone is another bis-sesquiterpenoid hypothesized to involve a Diels-Alder condensation reaction [78]. One of the protoilludene synthase associated gene clusters in Stereum may therefore encode a much sought after Diels-Alderase. The biosynthesis of melleolide antibiotics by the honey mushroom Armillaria mellea involves another unique modification of the protoilludene scaffold; esterification with a PKS derived orsellinic acid moiety (Figure 3 catalyzed by a cross-coupling orsellinic acid PKS [66].

Conclusions and Perspective

The past few years have seen a dramatic increase in genome sequence data available for Basdiomycota. Comparative genomic studies have provided important insights into the evolution and function of the complex lifestyles of this group of fungi. These studies and data give us access to a mostly unexplored source for new enzymes and biosynthetic pathways. Unlocking this resource requires significant efforts to overcome several challenges that currently slow down functional characterization of individual enzymes and the clustered secondary metabolic pathways of Basidiomycota. Most of these fungi are genetically intractable, preventing functional genetic studies feasible in other microbial organisms, including many Ascomycota. This leaves heterologous expression of genes and biosynthetic gene clusters as the only practical alternative. While genes can now be inexpensively synthesized and entire gene clusters rapidly assembled for heterologous expression, this approach from gene prediction to recombinant product is more often than not quite challenging for Basidiomycota. In our own experience, automated gene prediction pipelines frequently mis-annotate or fail to annotate genes altogether. Further, prediction of introns and exons using existing models are often incorrect, leading to non-functional genes if synthetically obtained rather than amplified from cDNA. The latter of course requires conditions under which a gene or pathway of interest is expressed. The development of accurate gene prediction and annotation pipelines will be required to take advantage of current synthetic biology capabilities to go from in silico gene/pathway prediction to recombinant product. In the future, automated high-throughput pipelines may become available to synthesize, assemble, express and screen libraries of oxidoreductases and pathways identified in fungal genomes. The development of alternative fungal hosts that are not yeasts and are amenable to rapid genetic engineering may also be required for functional expression biosynthetic pathways and enzymes from Basidiomycota.

Highlights

  • Unique lifestyles and ecologies of Basidiomycota are reflected in distinct portfolios of biocatalytic activities and secondary metabolic pathways.
  • Basidiomycota are masters of oxidative biocatalysis involving novel and expanded families of many different types of oxidoreductases.
  • Accelerated genome sequencing efforts provide resources to explore the biocatalytic capabilities of Basidiomycota.
  • Basidiomycota are prolific producers of bioactive sesquiterpenoid natural products and their genomes contain expanded families of sesquiterpene synthases.

Acknowledgments

Support by the National Institute of Health (grant GM080299) is acknowledged.

Footnotes

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