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Autophagy. 2016 May; 12(5): 900–904.
Published online 2016 March 17. doi:  10.1080/15548627.2016.1147673
PMCID: PMC4854548

Emerging nexus between RAB GTPases, autophagy and neurodegeneration

ABSTRACT

The RAB class of small GTPases includes the major regulators of intracellular communication, which are involved in vesicle generation through fusion and fission, and vesicular trafficking. RAB proteins also play an imperative role in neuronal maintenance and survival. Recent studies in the field of neurodegeneration have also highlighted the process of autophagy as being essential for neuronal maintenance. Here we review the emerging roles of RAB proteins in regulating macroautophagy and its impact in the context of neurodegenerative diseases.

KEYWORDS: autophagy, endocytosis, GTPase, intracellular trafficking, neurodegeneration, neuronal maintenance, RAB proteins, stress response

Intracellular trafficking is essential for neurons—a specialized type of cell that has long axonal processes that extend up to a few meters long—for maintaining polarity, stimulus dependent receptor uptake and degradation, and for axonal transport of organelles. RAB (Ras-genes from rat brain) GTPases are critical regulators that provide identity to vesicles and aid in the formation, movement and fusion of their respective membranes. RAB proteins thus play an indispensable role in neuronal processes such as NTF/neurotrophin-mediated signaling1 and thus consequently in neurite outgrowth.2 Another such active molecular process regulated by RAB proteins is macroautophagy (hereafter autophagy) a cellular catabolic process essential for neuronal maintenance. Defects in this pathway are strongly associated with several neurodegeneration disorders.3 The process of autophagy involves the formation of a double-layered membrane that engulfs the cellular contents to be degraded, followed by the fusion of the double-layered vesicle with a lysosome for cargo degradation. Classically, autophagy was viewed as being induced upon starvation, but recent literature highlights its importance as a housekeeping mechanism for clearing damaged organelles and potentially toxic protein aggregates. Thus, its requirement in cells of neuronal origin becomes important, and recent discoveries have shed light on various regulators, including a few on the role of a number of RAB proteins involved in multiple steps of autophagy.4 A number of studies also suggest the importance of RAB proteins in maintenance of healthy neurons, and how an overdose of RAB might reverse the phenotype inflicted by accumulation of misfolded proteins.5 Figure 1 provides a schematic view on the proposed role of the core autophagic machinery and its regulation by RAB proteins. In this article, we attempt to discuss isolated studies that ascribe aberration of this RAB-mediated autophagic regulation on the pathobiology of neurodegenerative diseases.

Figure 1.
Core autophagic machinery and its regulation by RAB proteins. Schematic diagram showing the role of the core autophagic machinery and its regulation by RAB proteins. 1. Induction: The initial phagophore assembly site (PAS) could be contributed to by any ...

RAB-regulated autophagy in neuronal survival

An early report suggested the requirement of RAB24 for survival of injured neurons.6 Both Rab24 and Map1lc3b/Lc3 gene transcripts were upregulated, and the 2 proteins colocalized in injured motor neurons as well as under proteotoxic stress induced by proteasomal blockade. The authors postulated RAB24-mediated autophagy to be required for nerve regeneration.6 A similar, activity-dependent relocalization of RAB24 was observed on pharmacological induction of autophagy, although in CHO cells.7 Recent studies have elegantly shown the role of RAB24 in autophagic clearance.8 Through the use of electron microscopy, it was suggested that it is not during the formation of the autophagosome that RAB24 plays a role. In fact, an early study had hinted at its role in maturation of autophagosomes as they reported the presence of RAB24 on late endocytic compartments.9 Overexpression of mutant HTT (huntingtin) protein with an expanded polyQ repeat in a defective RAB24 background leads to the accumulation of polyglutamine aggregates in HeLa cells.8 Thus, it would be safe to conclude that RAB24 not only plays a role in basal autophagy, but also helps in clearing the protein load through autophagy, thereby highlighting its importance in post-mitotic cell types, such as neurons. Supporting this finding was the genetic study of canine heredity ataxia that ascribed mutations in RAB24 to be a cause of the disease.10 The authors also described Purkinje neurons harboring a number of uncleared late autophagic vesicles.10 Thus, RAB24-mediated autophagy induction might protect neurons against cell death.

Abnormal RABs in neurodegenerative diseases

The importance of RABs in neuronal survival and maintenance became obvious with the identification of mutations in the RAB7A gene, which were found to be associated with the Charcot-Marie-Tooth disease type 2B, an axonal neuropathy.11 Cultured Purkinje neurons require the activity of RAB7A for efficient clearance of autophagosomes on trophic factor depletion.12 Specifically, RAB7A facilitates the crosstalk between endosomes and autophagosomes. An example of this crosstalk is the compensatory clearance of cargo by the other pathway if one is blocked. In the retina of Drosophila, for example, upregulation of RAB7A protein induced on blocking autophagy leads to compensatory degradation of ninaE/rhodopsin through endo-lysosomal degradation, thus preventing retinal degeneration.13 The significance of increased levels of RAB4A, RAB5A, RAB7A and RAB27A gene transcripts seen in brains of patients with Alzheimer disease (AD), or mild cognitive impairment is yet to be understood.14 Upregulation of RAB4A in AD, might be a consequence of AD-associated mitochondrial damage, as RAB4A is required for mitochondrial clearance in HeLa cells.15 RAB5A protein levels are also upregulated in mice models of Lafora disease, a neurodegenerative disorder associated with polyglucosan inclusions.16 Such a compensatory upregulation of RAB in pathologies such as Lafora disease could also be speculated. In brains of patients with dementia with Lewy bodies, large RAB7A-positive endosomes are seen, as well as increases in the protein level.17 The upregulation of RAB7A in such conditions could possibly represent a failed attempt for neuronal survival since MTOR (mechanistic target of rapamycin [serine/threonine kinase])-dependent autophagy induction (using a rapamycin analog) in a murine model of tauopathy also leads to an increase in RAB7A levels.18 A list of RAB proteins having a role in autophagy and mutated or misexpressed in neurodegenerative diseases is provided inTable 1.

Table 1.
List of neurodegenerative disorders with abnormal activities for RAB GTPase.

RAB regulators and their role in neurodegeneration

Functional studies on disease-associated proteins have led to the identification of novel regulators of RAB proteins and their involvement in autophagy. For example, wild-type HTT is required for movement of RAB7A-positive vesicles through neurons of a Drosophila model of Huntington disease.19 Any perturbation of HTT activity might thus impact RAB7A-mediated autophagy, and could be a cause of buildup of uncleared autophagosomes in Huntington disease. Another instance where a disease-associated protein regulates autophagy through RAB activity is that of LRRK1 (leucine-rich repeat kinase 1) involved in Parkinson disease (PD). Upon autophagic induction, LRRK1 mediates RAB7A GTPase activity through the regulation of TBC1D2—a RAB7A GTPase activating protein—and a loss in its activity leads to accumulation of autolysosomes and undegraded cargo.20 PARK2/Parkin, mutations in which are also associated with PD, also regulates the activity of the RAB-GTPase activating proteins TBC1D15 and TBC1D17, which are required for routing of damaged mitochondria to autophagosomes through RAB7.21 These proteins thus have an impact on the hydrolytic activity of RAB GTPases and thus might have a functional outcome on autophagy.

Crosstalk of ATG and RAB proteins

The biogenesis of the autophagosome requires the formation of double-membrane vesicles. Yet the aqueous intracellular environment would not favor de novo synthesis of a hydrophobic membrane. Therefore, the autophagosomal membrane precursor, the phagophore, is thought to originate from an intracellular organelle or a vesicle, onto which ATG proteins bind and induce membrane elongation/expansion. The organelle that contributes to the phagophore membrane in mammalian cells has been an enigma for a number of years. Recent discoveries, however, have established the contribution of almost all intracellular organelles. The different membrane sources reported for the biogenesis of the phagophore include the plasma membrane, recycling endosomes, endoplasmic reticulum, mitochondria and Golgi body.22 One can assume synaptic vesicles to be such a membrane donor as they employ RAB26-dependent autophagy elongation molecules: ATG16L1 and MAP1LC3B.23 In this regard, it is also interesting to note that early endosomal RAB5A-induced autophagy can lead to clearance of HTT aggregates, albeit in cellular and fly models of Huntington disease.24 The interaction between BECN1/Beclin 1, RAB5A, and PIK3C3/VPS34 is required for both the processes of autophagy as well as endocytosis.25 Based on this evidence we propose the partitioning of RAB5A into the autophagic elongation and early endosomal compartments, and channeling into each depending upon the specific cellular requirement. Thus, the RAB5A-decorated enlarged endosomes observed in models and patients of Alzheimer disease could be a consequence of decreased autophagy; in addition leading to enhanced generation of amyloid beta.26

RAB proteins rescue neurodegeneration by inducing autophagy

The possibility that RAB proteins could serve as stress response molecules comes from a study that demonstrated enhanced extracellular secretion of SNCA/alpha-synuclein through a RAB11A-dependent mechanism following an autophagy block.27 These examples do not come as a surprise, as overexpression of RAB proteins alleviates neurodegeneration in multiple models. For example overexpression of RAB11A in Drosophila models of Parkinson and Huntington diseases leads to a decrease of SNCA aggregates, reduced neuronal death and synaptic dysfunction.28,29 Since RAB11A plays a positive role in autophagic maturation,30 one could speculate that the alleviation of symptom is an outcome of induced autophagy.

Development of disease could be a consequence of RAB1A misfolding and inactivation as was seen in a model of amyotrophic lateral sclerosis.32 Mutant FUS (FUS RNA binding protein) is responsible for one of the familial forms of amyotrophic lateral sclerosis, and impairs autophagy. The impaired autophagic phenotype is reversed by overexpression of RAB1A in a mutant FUS background,34 thus underscoring the nexus between RABs, autophagy and neurodegeneration. Further studies on RAB proteins and RAB regulators in neurodegenerative disorders could offer insight into the complex process of autophagy and its role in neurodegeneration.

Abbreviations

AD
Alzheimer disease
ATG
autophagy related
DLB
dementia with lewy bodies
GTPase
guanosine-5′-triphosphatase
HD
Huntington disease
HTT
Huntingtin
LRRK1
leucine-rich repeat kinase 1
PAS
phagophore assembly site
PD
Parkinson disease
RAB
Ras related protein in brain
TBC
Tre-2, Bub2 and Cdc16 domain containing protein
ULK1
unc-51 like autophagy activating kinase 1

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

Acknowledgments

We wish to thank our colleague Ms. Neha Vithani for her help with the figure.

Funding

Work on autophagy in our laboratory is supported by a sponsored research grant from the Science and Engineering Research Board, Department of Science & Technology, Govt. of India (to SG).

References

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