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Gene Expr Patterns. Author manuscript; available in PMC 2017 March 9.
Published in final edited form as:
PMCID: PMC4842334
NIHMSID: NIHMS772110

Expression of Forkhead box transcription factor genes Foxp1 and Foxp2 during jaw development

Abstract

Development of the face is regulated by a large number of genes that are expressed in temporally and spatially specific patterns. While significant progress has been made on characterizing the genes that operate in the oral region of the face, those regulating development of the aboral (lateral) region remain largely unknown. Recently, we discovered that transcription factors LIM homeobox (LHX) 6 and LHX8, which are key regulators of oral development, repressed the expression of the genes encoding forkhead box transcription factors, Foxp1 and Foxp2, in the oral region. To gain insights into the potential role of the Foxp genes in region-specific development of the face, we examined their expression patterns in the first pharyngeal arch (primordium for the jaw) of mouse embryos at a high spatial and temporal resolution.

Foxp1 and Foxp2 were preferentially expressed in the aboral and posterior parts of the first pharyngeal arch, including the developing temporomandibular joint. Through double immunofluorescence and double fluorescent RNA in situ hybridization, we found that Foxp1 was expressed in the progenitor cells for the muscle, bone, and connective tissue. Foxp2 was expressed in subsets of bone and connective tissue progenitors but not in the myoblasts. Neither gene was expressed in the dental mesenchyme nor in the oral half of the palatal shelf undergoing extensive growth and morphogenesis. Together, we demonstrated for the first time that Foxp1 and Foxp2 are expressed during craniofacial development. Our data suggest that the Foxp genes may regulate development of the aboral and posterior regions of the jaw.

Keywords: Foxp1, Foxp2, craniofacial development, pharyngeal arch, temporomandibular joint, mouse

1. Introduction

The forkhead box (FOX) proteins are an ancient family of transcription factors, characterized by the conserved DNA binding domain called a forkhead domain (also known as a winged helix domain). They play important roles in a wide variety of biological processes, such as development, carcinogenesis, metabolism, and immunity (Hannenhalli and Kaestner 2009, Jackson, Carpenter et al. 2010, Lam et al. 2013). There are 50 members of FOX family in humans and 44 members in mice. The FOX proteins are further divided into subgroups, FOXA through FOXS, with 1-6 members in each subgroup (Jackson et al. 2010).

The FOXP subgroup is encoded by four genes (Foxp1-Foxp4), and they contain a leucine zipper motif and a zinc finger domain in addition to the forkhead domain (Takahashi et al. 2009). Among the four FOXP proteins, FOXP1 and FOXP2 show a particularly high homology to each other throughout their amino acid sequences (Takahashi et al. 2009). Foxp1 and Foxp2 are expressed in diverse organs during embryonic development, often in partially overlapping but distinct patterns (Shu et al. 2001, Wang et al. 2004, Shu et al. 2007, Takahashi et al. 2009, Leishman et al. 2013, Zhao et al. 2015). Analyses of mouse mutants have shown that Foxp1 plays critical roles in development of the spinal motor neurons, lymphocytes, and the cardiomyocytes (Wang et al. 2004, Hu et al. 2006, Dasen et al. 2008, Zhang et al. 2010). In addition, Foxp1 and Foxp2 co-regulate the development of the foregut and the skeleton (Shu et al. 2007, Zhao et al. 2015).

In humans, mutations affecting FOXP1 and FOXP2 have been found in patients with language and speech defects, often accompanied by additional intellectual disability and/or autism spectrum disorder (Lai et al. 2001, Feuk et al. 2006, Zeesman et al. 2006, Takahashi et al. 2009, Horn et al. 2010, Le Fevre et al. 2013, Lozano et al. 2015). Many of the patients also exhibited generalized functional deficiencies of the face and neck muscles (difficulties in chewing, swallowing, coughing, laughing), and characteristic facial features (triangular face, prominent forehead, short and broad nose, low set ears, downward slanting eyes, high-arched palate, wide-spaced teeth) (Feuk et al. 2006, Zeesman et al. 2006, Horn et al. 2010, Le Fevre et al. 2013, Lozano et al. 2015). The language and mental defects clearly implicate problems in brain development, and the musculoskeletal phenotypes of the face may be secondary to the disruption in the motor control from the brain for the muscles. Alternatively, the latter phenotypes can also be explained by abnormal development of the face itself. To date, potential roles of Foxp1 and Foxp2 in orofacial development have not been examined.

In vertebrates, the face develops from the embryonic primordia called frontonasal prominence and the first pharyngeal arches (PA1) (Minoux and Rijli 2010, Frisdal and Trainor 2014, Cesario et al. 2015a). PA1 gives rise to the jaw including the palate and the teeth, and it is further divided into the mandibular arch, which is the prospective lower jaw, and the maxillary arch, which is the prospective upper jaw. The mesenchyme of the developing face is of dual origin: the mesoderm-derived cells occupy the core of PA1, and they become skeletal muscles (Noden 1983, Noden and Trainor 2005). On the other hand, the cells that have migrated from the neural crest (called ectomesenchyme cells) give rise to all the bone and cartilage of the face, as well as connective tissues such as tendons and dermis (Noden 1978, Le Douarin and Kalcheim 1999).

Recently, we discovered that Lhx6 and Lhx8 repressed Foxp1 and Foxp2 in PA1, where the Lhx (LIM homeobox) genes are crucial to development of the palate and the molars on the oral side (Cesario et al. 2015b). To gain insights into the biological significance of the repression of Foxp by LHX, we examined the expression patterns of Foxp1 and Foxp2 during normal development of PA1 in mouse embryos. Our results suggest that the Foxp genes may be involved in development of the muscle, bone, and connective tissue in the face.

2. Results

At embryonic day (E) 10.5, Lhx6 and Lhx8 are expressed in the areas of PA1 facing the oral cavity, with the broad expression anteriorly (Fig. 1A; Grigoriou et al. 1998). On the other hand, the expression of Foxp1 and Foxp2 was excluded from the oral and anterior regions of PA1 (Fig. 1B,C).

Figure 1
Complementary patterns of expression between Lhx and Foxp genes in PA1

Examination on E11.5 sections revealed that Foxp1 was expressed in both the epithelium and the mesenchyme of PA1 (Fig. 1F,J,N). In the maxillary mesenchyme, Foxp1 expression was restricted to the dorsal-lateral (aboral) domain in the anterior and middle sections (Fig. 1F,J), complementary to the expression of Lhx6 and Lhx8 in the oral domain (Fig. 1D,E,H,I). This relationship was less pronounced in the posterior section (Fig. 1L-N). Foxp1 expression in the mandibular arch was mostly concentrated in and around the tongue (Fig. 1J,N). Foxp2 was expressed in the mesenchyme, but not the epithelium, of PA1 at E11.5, partially overlapping with Foxp1 (Fig. 1G,K,O). However, the expression domain of Foxp2 was significantly smaller than that of Foxp1.

At E12.5, Foxp1 was strongly expressed in the mesenchymal condensations in the lateral PA1 (dotted box in Fig. 2F). This area contained the primordia for the cheek bone (zygomatic arch), muscles of mastication (masseter), and the tendons between the bone and muscle, each marked by Runx2 (runt-related transcription factor 2, a marker of pre-osteoblasts; Karsenty 2008, Long 2012), MyoD1 (myogenic differentiation-1, a marker of myoblasts; Berkes and Tapscott 2005), and Scx (Scleraxis, a marker of tenocytes; Schweitzer et al. 2001) (Fig. 2H,I,J). Foxp1 was also abundantly expressed in the posterior PA1, where the maxillary arch and the mandibular arch joined (Fig. 2K). Foxp2 expression was strongest in the mesenchyme just under the eye (Fig. 2G), but low expression extended ventrally into the area where Foxp1 was expressed (arrow in Fig. 2G). In the posterior section, the expression of Foxp2 appeared nearly complementary to that of Foxp1 (Fig. 2L)

Figure 2
Expression of Foxp1 and Foxp2 in PA1 at E12.5

To confirm the expression of Foxp1 in the progenitors for the bone, muscle, and tendon, we used double immunofluorescence and double fluorescent RNA in situ hybridization to co-label FOXP1 protein or mRNA and the markers of each progenitor population. FOXP1 was detected in RUNX2+ cells in the dorsal part of the skeletogenic condensation for the zygomatic arch (Fig. 3A-C; arrow in C). In addition, FOXP1 was expressed in most of the MyoD1+ cells of the masseter although the intensity of FOXP1 showed a dorsal-to-ventral decline (Fig. 3D-E). Scx was expressed in the strip of cells between the RUNX2+ domain and MyoD1+ domain (Fig. 3B,E,H). Scx expression also partly overlapped with Runx2 at this stage (data not shown), which is reminiscent of the presence of Scx and Sox9 double-positive cells during early development of the tendon-bone attachment unit in the limbs (Blitz et al. 2013, Zelzer et al. 2014). Foxp1 mRNA was detected in a subset of Scx+ cells (Fig. 3G-I).

Figure 3
Co-expression analyses for the Foxp genes and the markers of the developing bone, tendon, and muscle at E12.5

Similar to FOXP1, FOXP2 was co-expressed with RUNX2 in the dorsal part of the zygomatic arch primordium (Fig. 3J-L; arrow in L). In addition, FOXP2, but not FOXP1, was expressed in some of the pre-osteoblasts in the developing palatine bone (dotted box in Fig. 2G; Fig. 3M-O). In contrast, FOXP2 was not expressed in the muscle cells (Fig. 3P-R). Limited overlap was detected between Foxp2 and Scx mRNA (Fig. 3S-U).

At E13.5, Foxp1 continued to be expressed in the mesenchymal mass of the developing zygomatic bone, masseter, and the tendons (Fig. 4G,I-L). In the posterior section, Foxp1 was expressed in the muscles and the surrounding connective tissues, labeled by MyoD1 and Scx (Fig. 4M,Q,R). RNA in situ hybridization on the sagittal sections of the head confirmed the overall pattern of Foxp1 expression identified from the coronal sections, namely, abundant in the lateral mesenchyme of PA1 (Fig. 4S) but minimal in the medial mesenchyme (Fig. 4X,c,h). Foxp2 continued to be expressed in the mesenchyme under the eye at E13.5, but with sharp boundaries instead of the ventral tail seen at E12.5 (Fig. 4H). The intensity was also significantly reduced. Foxp2 expression appeared to be mostly in the undifferentiated mesenchyme that did not express Runx2, Sp7 (also known as Osterix, an osteogenic transcription factor acting downstream of RUNX2; Nakashima et al. 2002, Long 2012), Scx, nor MyoD1 (Fig. 4).

Figure 4
Expression of Foxp1 and Foxp2 in PA1 at E13.5

Double immunofluorescence showed that the overlap between FOXP1 and RUNX2 increased substantially at E13.5 compared with E12.5 (Fig. 5A-C). FOXP1 was also co-expressed with SP7 (Fig. 5D-F). Almost all the RUNX2+ cells and OSX+ cells appeared to express FOXP1 although the level of FOXP1 varied widely within the population of cells. Similarly, Foxp1 mRNA was detected more abundantly in Scx+ cells at this stage than E12.5 (Fig. 5G-I).

Figure 5
Co-expression analyses for the Foxp genes and the markers of the developing bone and tendon at E13.5

At E13.5, FOXP2 was expressed only in a small subset of the pre-osteoblasts for the zygomatic bone (Fig. 5J-O). Also, in the developing palatine, the levels of FOXP2 protein were sharply reduced in the cells co-expressing RUNX2 and SP7, which correspond to more differentiated cells than RUNX2+ SP7- cells (Fig. 5P-U).

Foxp1 was expressed in the head muscles and the tendons surrounding them at E14.5 (Fig. 6A,F,K,P), but its expression in the zygomatic arch was down-regulated compared with the earlier stages (Fig. 6A,F). Foxp2 expression was further reduced, and it was maintained only in small areas just under the eye and at the root of the tongue in the anterior sections (Fig. 6B,G). In the posterior section, the developing temporo-mandibular joint (TMJ) was recognizable for the first time at this stage. It appeared as the osteogenic condensations for the glenoid fossa of the temporal (squamosal) bone and the condyle of the dentary bone (Fig. 6R), with the intervening mesenchyme that will form the fibrocartilagenous articular disc (arrowhead in Fig. 6S). Both Foxp1 and Foxp2 were expressed in this mesenchyme between the glenoid fossa and the condyle (arrowheads in Fig. 6P,Q).

Figure 6
Expression of Foxp1 and Foxp2 in PA1 at E14.5

At E15.5, only faint expression of Foxp1 and Foxp2 was detected in the jaw mesenchyme (Fig. 7). Foxp1 expression was noticeable in the muscles and the tendons (arrows in Fig. 7B,H). In addition, as the articular disc of TMJ takes shape (arrowhead in Fig. 7G), Foxp1 was specifically expressed in the disc (arrowhead in Fig. 7H). Foxp2 was also expressed in the disc area (arrowhead in Fig. 7I). However, Foxp2 was expressed in the mesenchyme around the condyle in all directions instead of being localized to the interface with the glenoid fossa (Fig. 7I). The expression of Foxp1 and Foxp2 were no longer detectable in the facial mesenchyme at E16.5 (data now shown).

Figure 7
Expression of Foxp1 and Foxp2 in PA1 at E15.5

To summarize, we found that Foxp1 was expressed in the developing zygomatic arch bone, the muscles and tendons of the jaw, and the articular disc of TMJ. Foxp1 expression in the bone was dynamic, being most robust at E13.5. Foxp2 showed limited expression in the zygomatic arch and palatine bone at earlier stages, but it was not expressed in the muscles. Both Foxp1 and Foxp2 were down-regulated later in gestation.

It is noteworthy that the expression of Foxp1 and Foxp2 was absent from the odontogenic mesenchyme of all the teeth (Fig. 2F,G; Fig. 4A,B,G,H,c,d; Fig. 7B,C), and also from the mesenchyme of the ventral (oral) domain of the palatal shelf during the major growth and morphogenesis of the palate (Fig. 2A,B,F,G,K,L; Fig. 4A,B,G,H,M,N). In addition, we failed to detect any Foxp1 or Foxp2 expression in the developing dentary bone of the mandibular arch.

3. Discussion

In this report, we describe the expression of Foxp1 and Foxp2 during craniofacial development for the first time. Our data suggest that Foxp1 may regulate development of the muscular, skeletal, and connective tissue in the aboral part of the face, including the TMJ. While we know of many genes that are expressed in the oral mesenchyme and play essential roles in development of the palate and/or teeth (Bei 2009, Bush and Jiang 2012), little is known about the factors that regulate aboral development. Therefore, Foxp1 is highly unique among the genes implicated in craniofacial development. Another unusual feature of Foxp1 is that it is expressed in both neural crest-derived cells (precursors of bone and tendon) and mesoderm-derived cells (myoblasts) within PA1 mesenchyme, whereas most genes are expressed in one of the two lineages only (Cesario et al. 2015a). Unlike Foxp1, Foxp2 expression was specific to the neural crest-derived tissue, some in the osteogenic condensations and the connective tissue, but mostly in the undifferentiated mesenchyme.

Foxp1 and Foxp2 have been implicated in bone development in other parts of the body. During endochondral ossification of the long bones, FOXP1 and FOXP2 suppressed osteoblast differentiation by inhibiting the activity of RUNX2 through physical interactions (Zhao et al. 2015). Surprisingly, however, deletion of Foxp1 and Foxp2 in the calvaria led to deficient ossification, indicating that the FOXP proteins are positive regulators of bone formation in this context (Zhao et al. 2015). The bones of the face are similar to the calvarial bone in that both develop intra-membraneously. Therefore, we speculate that Foxp1 and Foxp2 may be required for the normal development of the zygomatic arch and the palatine bone from PA1. However, the fact that two genes were down-regulated in the osteogenic condensations after E13.5 suggests that their role would be limited to the early specification of the cell fate.

In addition to the bone, Foxp1 has also been implicated in tendon and muscle development. Whole transcriptome profiling of limb tendons identified Foxp1 as one of the genes activated during early tendon development (Liu et al. 2015). In the heart, Foxp1 was required cell-autonomously to promote differentiation and repress proliferation of the cardiomyocytes (Zhang et al. 2010). Cardiomyocytes and the skeletal muscles of the pharyngeal arches (branchiomeric muscles) develop from a common pool of mesodermal progenitors termed “cardiopharyngeal field”, and are regulated by many of the same genes (Tzahor 2009, Diogo et al. 2015). Therefore, it is plausible that Foxp1 plays a similar role in the muscles of the heart and the jaw.

In conclusion, we have demonstrated that Foxp1 and Foxp2 are expressed in the developing face. Given that human patients with mutations in FOXP1 or FOXP2 frequently exhibit dysfunction and dysmorphology of the orofacial region, future investigation is necessary to elucidate the function of the Foxp genes in craniofacial development.

4. Experimental Procedures

4.1. Preparation of tissue

All the tissue sections used in this study are frozen sections. Wild type mouse embryos of mixed strain background were used. All the experiments using mice were performed following protocols approved by New York University institutional committee on the use of laboratory animals, and in accordance with the National Institutes of Health guide for the care and use of laboratory animals. Tissue sections were prepared as previously described (Jeong et al. 2012). Some sections were stained with cresyl violet to visualize tissue morphology as previously described (Jeong et al. 2012).

4.2. RNA In situ hybridization

For in situ hybridization for a single gene, antisense probes were labeled with digoxigenin (DIG RNA labeling mix, Roche) and the hybridization was performed following a standard protocol (Schaeren-Wiemers and Gerfin-Moser 1993). After incubating with anti-digoxigenin antibody conjugated with alkaline phosphatase (Roche), BM purple (Roche) was used to visualize the signal.

For double fluorescent in situ hybridization, Tyramide Signal Amplification (TSA) system (PerkinElmer) was used. One antisense probe was labeled with digoxigenin, and the other probe was labeled with fluorescein (Fluorescein RNA labeling mix, Roche). Two probes were mixed and added to the sections. After unbound probes were washed away, the slides were first incubated with anti-digoxigenin antibody conjugated with peroxidase (Roche). Biotin-tyramide → streptavidin-peroxidase → Cy3-tyramide were added sequentially, which allowed the detection of the digoxigenin-labeled probe through Cy3 fluorescence. The peroxidase reaction was then quenched by incubating the slides in 3% hydrogen peroxide. Next, the slides were treated with anti-fluorescein-peroxidase antibody (Roche) → fluorescein-tyramide → anti-fluorescein-peroxidase antibody → fluorescein-tyramide sequentially, to amplify the signal and visualize the fluorescein-labeled probe. The nuclei were stained with DAPI.

The plasmid templates for Lhx6, Lhx8, Runx2, Sp7, and MyoD1 probes were obtained from other researchers (Davis et al. 1987, Ducy et al. 1997, Grigoriou et al. 1998, Nakashima et al. 2002). For Scx, a full length cDNA clone (NCBI accession # BC062161) was purchased from GE Dharmacon, and it was linearized and used for in vitro transcription. For Foxp1 and Foxp2 probes, full-length cDNA plasmids were obtained from another laboratory (Shu et al. 2001), and parts of them were amplified by PCR and used as templates. The PCR primers for Foxp1 were 5’-AGCAACCAGCTCTTCAGGTTCC-3’ and 5’-AATTAACCCTCACTAAAGGGACGTTGTATTTGTCTGAGTACCG-3’ (underlined is T3 polymerase promoter); the amplified region is nucleotide 500-1563 of NCBI accession # BC064764. The PCR primers for Foxp2 were 5’-CTGTGATGTTGCAGCAGCAGCA-3’ and 5’-AATTAACCCTCACTAAAGGGGAAGGGAGGTCTAACATCTGCG-3’; the amplified region is nucleotide 699-1774 of NCBI accession # BC058960. While Foxp1 and Foxp2 have large numbers of isoforms (10 and 5, respectively, according to University of California Santa Cruz Genome Browser), the probes were designed against the regions common to almost all the isoforms. Therefore, our in situ hybridization likely detected all of Foxp1 and Foxp2 expression in the region irrespective of the isoforms.

4.3. Immunofluorescence

Immunofluorescence was performed as described (Jeong and McMahon 2005). The primary antibodies were: goat polyclonal anti-FOXP1 (R&D Systems, catalog # AF4534, 1:300), goat polyclonal anti-FOXP2 (Abcam, catalog # ab1307, 1:1000), rabbit polyclonal anti-MyoD (C-20) (Santa Cruz Biotech, catalog # sc-304, 1:100), rabbit polyclonal anti-RUNX2 (Santa Cruz Biotech, catalog # sc-10758X, 1:500), and rabbit polyclonal anti-Sp7 (Abcam, catalog # ab22552, 1:150). All the secondary antibodies were Alexa Fluor antibodies from Life Technologies. DAPI was used to stain the nuclei.

Highlights

  • We describe the expression of Foxp1 and Foxp2 during craniofacial development.
  • Foxp1 was expressed in the developing muscle, bone, and tendon of the jaw.
  • Foxp2 was expressed in a subset of bone and tendon progenitors.
  • Both Foxp1 and Foxp2 were expressed in the temporomandibular joint.
  • This is the first report on the expression of Foxp1 and Foxp2 in the face.

Acknowledgments

This work was funded by National Institute of Dental and Craniofacial Research, National Institutes of Health (R00 DE019486 to J.J.).

Footnotes

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