Search tips
Search criteria 


Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Dev Biol. Author manuscript; available in PMC 2017 May 1.
Published in final edited form as:
PMCID: PMC4834247

In vivo time-lapse imaging reveals extensive neural crest and endothelial cell interactions during neural crest migration and formation of the dorsal root and sympathetic ganglia


During amniote embryogenesis the nervous and vascular systems interact in a process that significantly affects the respective morphogenesis of each network by forming a “neurovascular” link. The importance of neurovascular cross-talk in the central nervous system has recently come into focus with the growing awareness that these two systems interact extensively both during development, in the stem-cell niche, and in neurodegenerative conditions such as Alzheimer’s Disease and Amyotrophic Lateral Sclerosis. With respect to the peripheral nervous system, however, there have been no live, real-time investigations of the potential relationship between these two developing systems. To address this deficit, we used multispectral 4D time-lapse imaging in a transgenic quail model in which endothelial cells (ECs) express a yellow fluorescent marker, while neural crest cells (NCCs) express an electroporated red fluorescent marker. We monitored EC and NCC migration in real-time during formation of the peripheral nervous system. Our time-lapse recordings indicate that NCCs and ECs are physically juxtaposed and dynamically interact at multiple locations along their trajectories. These interactions are stereotypical and occur at precise anatomical locations along the NCC migratory pathway. NCCs migrate alongside the posterior surface of developing intersomitic vessels, but fail to cross these continuous streams of motile ECs. NCCs change their morphology and migration trajectory when they encounter gaps in the developing vasculature. Within the nascent dorsal root ganglion, proximity to ECs causes filopodial retraction which curtails forward persistence of NCC motility. Overall, our time-lapse recordings support the conclusion that primary vascular networks substantially influence the distribution and migratory behavior of NCCs and the patterned formation of dorsal root and sympathetic ganglia.

Keywords: Neural crest migration, endothelial cells, peripheral nervous system, neurovascular

Graphical abstract

Synopsis of spatial-temporal interactions between neural crest cells (NCCs; red) and endothelial cells (ECs; green) during trunk neural crest migration. Longitudinal view of neural tube depicts the anterior-posterior gradient in neural crest migration. Numbers 1–7 indicate discrete locations wherein stereotyped interactions occur between NCCs and ECs: (1) The density of ECs is asymmetrically distributed along the length of the dorsal surface of the somite, with an increased accumulation of ECs dorsal to the posterior half-somite (rather than the anterior half-somite). This places these ECs immediately ventral to the NCCs in the MSA with which they interact. (2) Within the intersomitic furrow, NCCs track along the posterior surface of the developing ISV, and do not cross the EC stream. (3) NCCs tracking along the ISV change their ventrolateral trajectory upon contact with the PCV to colonize discrete SG. (4) Pioneer Neurogenin2+ NCCs that seed the DRG migrate ventrally until they contact ECs in the PSV. Later arriving NCCs then backfill the DRG from that ventral-most location. (5) As ECs fill in the fissure of von Ebner, they contact NCCs in the most lateral perimeter of the DRG. (6) ECs associate with motor and sensory axons in the emerging spinal nerves and eventually surround them as the nerves segregate axially. (7) As development continues, ECs gradually circumscribe the entire DRG, constraining NCCs within the ganglion and driving the proliferation of progenitor cells in the DRG perimeter. DA, dorsal aorta; DRG, dorsal root ganglion; ISV, intersomitic vessel; MSA, migratory staging area; NT, neural tube; PCV, postcardinal vein; PNVP, perineural vascular plexus; PSV, perisomitic vessel; SG, sympathetic ganglion.

An external file that holds a picture, illustration, etc.
Object name is nihms769136u1.jpg


How a precisely patterned peripheral nervous system (PNS) emerges from a heterogeneous population of neural crest cells remains a fundamental question in developmental biology. Trunk NCCs undergo an epithelial-to-mesenchymal transition to delaminate from the neural tube and then migrate ventrally and laterally through the embryo: those NCCs that stop next to the dorsal aorta differentiate into the paravertebral chain of sympathetic ganglia (SG) while those that stop adjacent to the neural tube give rise to the dorsal root ganglia (DRG) (14). The cues that guide NCCs to preferentially colonize either the presumptive SG or DRG are not fully understood. NCCs delaminate in a temporal sequence that corresponds to their migratory route and cell fate, with NCCs that form the SG exiting the neural tube prior to those that form the DRG (19). The earliest, SG-fated NCCs migrate ventrally either between the somites or between the neural tube and somite and differentiate directly into neurons, while later SG-destined NCCs migrate along the ventral border of the dermomyotome and through the sclerotome to form a perimeter composed of mitotically-active progenitor cells (10). This change in migration pathway is likely mediated by SEMA3A and NRP1, since an increase in NCC migration through the intersomitic furrow is observed when the expression of either of these genes is ablated in the somite (11). All NCCs destined for the DRG associate with the sclerotome (6, 8, 12), with the last NCCs to colonize the ganglion also forming a perimeter of mitotically active progenitors (5, 13). An interesting spatial dynamic of trunk NCC migration is the fact that although NCCs delaminate along the entire length of the neural tube, they migrate rostrally and/or caudally within the migration staging area (MSA) (14) before penetrating the anterior halves of each somite, in part due to repulsive EphrinB and Sema3F cues expressed in the posterior somite half (15, 16). In addition to cell membrane mediated guidance cues, NCCs destined to colonize the SG selectively express CXCR4 and chemotax towards periaortic sources of SDF-1 (10, 17).

Most studies of NCC migration have relied on static analyses to discern intercellular interactions. However, live time-lapse imaging of intact embryos and explants has revealed novel NCC behaviors (5, 13, 16, 1832). These studies have shown that NCCs are highly dynamic as they migrate, extending and retracting filopodia and moving in all four directions (dorsal, ventral, rostral, and caudal) as they interact with cues in their environment. Most NCC studies also tend to be “neural crest-centric”, that is they do not describe the surrounding non-neural crest cell types with which NCCs interact along their migratory routes. Other than the clear effect the anterior versus posterior somite plays in patterning NCC migration (11, 15, 33, 34), the potential influence of other non-NCCs on NCC behavior has not received much attention. In the trunk, a major cell type migrating at the same time and place as NCCs, are ECs as they form the embryonic vasculature. Static analyses in the trunk have demonstrated the colocalization of NCCs and ECs in the intersomitic furrow, around the dorsal aorta and postcardinal veins, and along the longitudinal anastomosis of the neural tube (9, 11, 35, 36). In the developing enteric nervous system (ENS), while one study did report a dependency of NCC migration in the hindgut along blood vessels (37), other studies in both chick and mouse have found that the vascular and ENS systems develop autonomously or do not spatially align (30, 3840). A study in zebrafish found no affect on DRG positioning in the absence of normal vascular patterning (35).

An emerging focus in both neurodevelopment and neurodegenerative disease is the “neurovascular link”: that is, the recognition of extensive intercellular signaling between blood vessels, in particular, ECs and neurons, axons, and glia (4147). The close congruency of blood vessel and peripheral nerve growth is well documented (45, 46, 48), and numerous studies have shown that the developing vascular and peripheral nervous systems share guidance cues (4951). For example, blood vessels often track alongside peripheral nerves, while signals from both blood vessels and nerves can promote the branching and survival of each other (5254). In the developing central nervous system (CNS), blood vessels have been shown to act as a substrate for migrating neuroblasts and olfactory bulb interneurons (5557). In addition, the development, maintenance and proper functioning of both the nervous and vascular systems have been shown to be mutually interdependent via the secretion of growth factors including NGF, BDNF and VEGF (11, 43, 5865). Neurons and endothelial cells share additional molecular pathways including Ephrins/Ephs; Nrpns/Semas/Plexins, Netrins, Notch/Delta, Slit/Robo, and SDF-1/CXCR4 (10, 6673). In the CNS, these bidirectional interactions are such that they comprise a “neurovascular unit” that includes neurons, glia, pericytes, and ECs, and this ensemble provides a critical stem cell niche in which mitotically active neural progenitors are found in close proximity to blood vessels (41, 74). Crosstalk between the two systems promotes the survival and maintenance of neurons and ECs and in fact, the neural degeneration that is the hallmark of many neurological diseases (e.g. Alzheimer’s, Amyotrophic Lateral Sclerosis) is postulated to result from disruptions in this crosstalk (64).

Given the significance of neurovascular interactions in other regions of the developing nervous system, we sought to investigate a potential “neurovascular link” during the formation of the trunk peripheral nervous system. To capitalize on the enhanced perspective provided by live imaging techniques, we used a line of transgenic quail, Tg(tie1:H2B-eYFP), in which endothelial cells are genetically engineered to express nuclear-localized enhanced yellow fluorescent protein (H2B-eYFP) (75). By simultaneously imaging NCCs tagged with monomeric cherry fluorescent protein (ChFP), and eYFP+ ECs, we recorded these two distinct cells types interacting with each other in real time during the formation and patterning of the PNS. Our work reveals the extensive nature of these interactions and provides evidence for interconnectedness and interdependence between the nervous and vascular systems as they develop concurrently within the embryo.

Materials and Methods

Quail embryos

The generation of a tie1 transgenic quail line Tg(tie1:H2B-eYFP) has been previously described (75) as well as our methods for electroporation of avian embryos (13). Briefly, for fluorescent labeling of NCCs, Japanese quail, (Coturnix coturnix japonica) embryos were incubated until Hamburger and Hamilton Stage (St.) 10–11 (76), and the fluorophore expressing plasmid, plenti-PGK:mCherry (77), was injected at a concentration of 0.25 μg/μl in PBS until the entire length of the neural tube was filled. Electroporator settings were 5 pulses and 23 V with an interval of 100 ms.

Immunohistochemistry (IHC)

Detailed methods for performing IHC using 16 μm cryosections, including embryo preparation, sectioning, and immunostaining, have been previously described (13). Unless otherwise stated, all IHC images correspond to the mid-trunk axial level. Mouse monoclonal primary antibodies anti-HNK1(IgM) (1:25) and anti-neurogenin2 (NGN2) (1:30,000) were gifts from Dr. Ben Novitch, UCLA. Anti-TUJ1 monoclonal mouse antibody (1:1000) was obtained from Covance (MMS-435P). Mouse monoclonal antibodies anti-PAX3, anti-Laminin, and anti-QH1 were obtained from the Developmental Studies Hybridoma Bank ( and used at a final concentration of 1 μg/ml. Rabbit polyclonal antibody anti-phospho-histone H3 (pH3) was obtained from Millipore (06-570).

For whole mount IHC, embryos were fixed in 4% PFA/PBS for 4–6 hours (depending on age), and rinsed in PBS. For antibody staining, embryos were placed in scintillation vials and bathed in PBST (PBS and 0.5% Triton X-100) for 40 minutes, followed by NGS block (20% normal goat serum, 1% glycine, 0.5% Triton X-100 in 30 mM Tris, 150 mM NaCl) for 1 hour, and incubated for 4 days with mouse monoclonal primary antibodies anti-HNK1 IgM (1 μg/ml) and anti-QH1 IgG (1 μg/ml) in NGS block. Embryos were then rinsed for 30 minutes in NGS block, rinsed for 5 hours in PBST, and incubated for 1 hour with NGS block prior to incubation in secondary antibodies [Goat anti-Mouse IgM, Alexa Fluor® 568 at 1:2000 (Thermo Fisher Scientific); Goat anti-Mouse IgG Fcγ 488 at 1:300 (Jackson Immuno Research Labs)] in NGS block for 6 days. Embryos were then rinsed in PBST and stored overnight in PBS at 4°C.


All static fluorescent images with the exception of Fig. 3A, B were captured using an inverted laser scanning confocal microscope (Olympus FV300) with 488 nm, 543 nm, and 633 nm laser lines and Fluoview Software v. 5.0. Objectives included a 20X Plan Apo (NA = 0.70) dry objective and a 60X Plan Apo (NA = 1.4) oil immersion lens (Olympus). Sections shown in 1B, 2B, 4C, 5A–F, and 6A, B, D were 16 μm.

Figure 3
Endothelial cell distribution may influence NCC migration and contribute to the rostral/caudal patterning of the peripheral nervous system. A–D) St.15. A, B) Individual frames from Movies 3A and 3C, respectively, showing a live, whole mount embryo ...

For Movies 1A,B, the trunks of immunostained whole embryos were cut in half sagittally using a feather blade under a dissecting scope. For Movies 1A–C, embryos were rendered optically transparent (cleared) prior to imaging using a methyl salicylate and benzyl benzoate (MSBB) method described previously (78). Briefly, embryos were dehydrated using a series of ethanol solutions in distilled water (70%, 95%, 100%), and placed in a methyl salicylate, benzyl benzoate (5:3) solution for 30 minutes. All 3D images were acquired using the FV300 confocal and 20X objective described above, with a lateral (x–y) resolution of 0.69 μm per pixel and optically sectioned at 2 μm in Z. Confocal images were converted to 3D tiff-stacks using ImageJ (, and then imported to Osirix imaging software ( to generate rotational and fly thru movies (Movies 1A–C).

For time-lapse imaging in Movies 2A,B, and photomicrographs shown in Figures 2A and 3F,G, whole embryos were mounted on paper rings and transferred to Millicell-CM 0.4 mm culture plate inserts (Millipore, Bedford, MA) with the legs removed and saturated in neurobasal media (Invitrogen) supplemented with B27 (Invitrogen) within a 35 mm glass bottom dish (MatTek, Ashland, MA). Images of a single focal plane were captured every 5 minutes using the 20X dry objective on our FV300 (above) within an enclosure heated to 37°C. For Movies 3E–G, 4, 5, 6, and Figures 3E, H–K, and and4B4B showing transverse slice preparations, embryos were dissected in sterile, ice cold 1xPBS, embedded into low melting point agarose and sectioned at 250 μm using a vibratome. Transverse slices were then transferred to Millicell-CM 0.4 mm culture plate inserts (Millipore, Bedford, MA) saturated in neurobasal media (Invitrogen) supplemented with B27 (Invitrogen). The Millicell membrane with slice preparation was then cut from the plastic insert and placed in a 35 mm glass bottom dish (MatTek, Ashland, MA). Single focal plane images were captured every 5 minutes using the 20X objective on our FV300 within a heated enclosure.

Figure 2
Neural crest cells interact extensively with both endothelial cells and the somitic mesoderm during early neural crest cell migration. A, B) St. 16 Tg(tie1:H2B-eYFP) quail. A) Frame 1 from Movie 2B of a live, whole mount embryo viewed from the lateral ...
Figure 4
Neural crest cells track along the developing intersomitic vessel to colonize the sympathetic ganglia. A) Schematic of a transverse section through the trunk of a St. 17 quail embryo. The dashed line with arrowheads depicts the migration pattern of NCCs. ...

For time-lapse imaging in Movies 2C and 3A–D embryos were maintained ex ovo by mounting the specimens on paper rings as previously described (79, 80) and transferred to the environmentally controlled stage of a conventional microscope and wide-field time-lapse recordings were performed using an automated microscopy system; the instrumentation and methods have been described in detail elsewhere (8083). Twelve-bit gray-scale images were recorded every 7 minutes in both DIC and epifluorescence modes. Images were acquired at 7–9 focal planes, separated by 10 μm, and in most cases at multiple XY positions arranged as overlapping image tiles. This procedure over-sampled the specimen in X-Y-Z space thus ensuring that any point of interest remained in focus despite the normal movements of the embryo over the recording period (8–20 hours). The imaging system was based on a computer-controlled standard compound Leica DMR inverted (Movie 2C) or upright (Movies 3A–D) microscope equipped with a motorized stage and a QImaging Retiga-SRV TM camera, using a Leica 506057 N PLAN L 20X/0.40 CORR “Infinity Symbol”/0-2C objective (Movie 2C), or a Leica 556000 PL Fluotar 10X/0.25 P “Infinity symbol”/- objective (Movies 3A–D). Freely available, open source software code of our design (TiLa, KUMC) was used to process, align, smoothen and register the adjacent XY image tiles, from algorithm-selected Z planes, to produce a full-scale composite image for each time point (i.e., one movie frame). The resulting sequential image frames were concatenated and compiled as a movie file using ImageJ, then converted to MP4 format and annotated using Adobe Premiere Elements 14.

Quantification of EC density above the anterior versus posterior half somite

Time lapse recordings of St. 15 quail embryos obtained via the methods described above for Movies 3A–D were used to quantify EC density. The fissure of von Ebner was used to delineate the anterior and posterior halves of somites 6–12, and the number of ECs present above each half was counted every 10 frames (or 70 minutes) over the course of 105 frames (or ~12 hours), n = 4 embryos.

Quantification of proliferation

Data shown in Figure 6C were obtained by counting the number of mitotically active (pH3+) and non-mitotic (pH3) DRG progenitor cells (HNK1+) within the DRG perimeter (outer-most cell layer only) that were in contact with ECs (QH1+). DRG from 3 axial levels including the wing, trunk, and hind limb were included with no significant differences found between axial levels. For each DRG, the center section was determined, and the two sections flanking the center section were counted. For St. 26, 57 sections from 3 embryos were counted. For St. 22, 55 sections from 3 embryos were counted.

Figure 6
Contact with endothelial cells may influence the mitotic activity of dorsal root ganglia progenitors. A, B, D) St. 26/27, 16 μm transverse cryosections. A, B) Adjacent serial sections show that PAX3+ progenitors within the dorsal pole of the DRG ...


At the level of the wing bud, NCCs begin delaminating from the neural tube at St. 13 and migrate ventrally both through the intersomitic furrow and between the neural tube and somite to form the SG (1, 3, 9). The next cohort of delaminating NCCs (between St. 14–19) migrates ventrally to form the SG perimeter (10), or stops next to the neural tube to form the DRG (1). The third cohort to migrate ventrally (St. 17–20) does so either ipsilaterally or contralaterally to form the DRG perimeter (5), while the last cohort (St. 19–21) traverses laterally into the ectoderm to form melanocytes (2, 8486).

Migrating neural crest cells and the nascent peripheral ganglia are tightly juxtaposed to the developing vasculature

To determine the position of migrating trunk NCCs and their derivatives relative to the developing vasculature, we fluorescently labeled both systems in fixed embryos at three developmental time points, collected images at successive focal planes throughout the trunk, and reconstructed these images three dimensionally. By St. 23, NCC migration is complete and the DRG and SG are composed of differentiating neurons and mitotically active neural progenitors (13). Movie 1A, a medial to lateral projection through the trunk of a St. 23 embryo, and Figures 1A, B show a striking spatial apposition of the nascent sensory and sympathetic ganglia with the developing vasculature. NCC derivatives in the rostral most region of each DRG anlage are tightly apposed to ECs in the intersomitic vessel (ISV), while cells in the caudal region of each DRG anlage are in contact with ECs within the fissure of von Ebner that forms at the anterior/posterior midpoint of the somite (87). In fact, as shown in Figures 1A, B, the anlagen appear completely circumscribed by ECs and a network of blood vessels that by now also surrounds each developing peripheral nerve. In particular, these reconstructions identify several potential NCC behaviors, including their migration and consolidation, that could be influenced by ECs.

Figure 1
The developing sensory and sympathetic ganglia are tightly juxtaposed to the developing vasculature. A–D) Neural crest derivatives including the DRG and SG were labeled with HNK1 and the developing vasculature with QH1. A,B) By St. 23 the DRG ...

We have previously documented the active segregation of NCCs as they form the sympathetic ganglia chain and that this process is dependent on EphB2/EphrinB1 interactions (21). Movie 1B and Figure 1C of a St. 18 embryo demonstrates that the interganglionic area that we previously showed expresses EphrinB1, is in fact replete with a high density of ECs (arrows in Fig. 1C). Given that ECs are known to express EphrinB1 (16), this raises the possibility that ECs are the source of EphrinB1 that drives the segregation of putative SG cells in the interganglionic region.

Movie 1C, a dorsal to ventral progression through the trunk of a St. 15 quail embryo, shows that the MSA where NCCs pause and accumulate subsequent to undergoing EMT but prior to ventral migration, positions NCCs directly dorsal to and juxtaposed to the developing vertebral blood vessels (36). Movie 1C and Figure 1D also show that NCCs are tightly apposed to ECs that comprise the ISV as they enter the intersomitic furrow to migrate ventrally between the ISV and the anterior face of the somite.

Early neural crest cells migrating within the intersomitic furrow interact extensively with endothelial cells and the anterior face of the somite

Having established the close physical proximity of ECs and NCCs in static reconstructions, we next sought to dynamically investigate the locations, migration patterns and behaviors of NCCs and ECs, relative to the somites, during the emergence of the trunk peripheral nervous system, specifically during development of the dorsal root and sympathetic ganglia.

The intersomitic vessels form by branching off of the dorsal aorta and postcardinal vein (88, 89). Given the spatiotemporal proximity of early migrating NCCs and ECs within the intersomitic furrow where the ISV will form, we first characterized the interactions of these two cell types using time-lapse confocal microscopy of eYFP+ ECs [Tg (tie1:H2B-eYFP)] (75, 90) and ChFP+ NCCs in whole mount embryos between St. 15–17. These time-lapses demonstrate that as NCCs move out of the MSA to migrate ventrally within the intersomitic furrow, they appear to migrate down and even track along the posterior side of the EC stream (Fig. 2A and Movies 2A, B). To visualize membrane-to-membrane contact between these two cell types and to determine whether both cell populations move through the same longitudinal plane, we analyzed static longitudinal sections of quail embryos immunostained with QH1 antibody to visualize EC membranes, and HNK1 antibody to visualize NCC membranes. In these sections, early-migrating NCCs and ECs are tightly juxtaposed, making extensive intercellular contact within the intersomitic furrow (Fig. 2B). At these stages, the anterior half somite in quail embryos is somewhat HNK1 immunoreactive (34), allowing us to also visualize the proximity of NCCs to the anterior somite face. As shown in Figure 2B, NCCs are tightly apposed to ECs on their rostral side, and to the anterior face of the somite on their caudal side. Movie 2C overlays fluorescent time-lapse images with differential interference contrast (DIC), allowing for visualization of the somitic environment, as well as migrating NCCs and ECs. The DIC channel is also shown in grayscale to provide an enhanced perspective of the somites and the size of the intersomitic furrow. These time-lapses and static images demonstrate that NCCs make extensive contact with both ECs and somitic mesoderm as they enter and migrate through the ISV.

Nascent vascular patterning demarcates neural crest migration boundaries

Our 3D reconstructions, live imaging, and analysis of static tissue sections demonstrate that early NCCs migrating between the somites move ventrally between rostrally adjacent ECs and the caudally adjacent anterior somite. NCCs were rarely observed on the anterior side of the EC stream or crossing the dorsal/ventral axis of the stream in the anterior direction (28 out of 844 migrating NCCs were observed on the anterior side of the developing ISV, n = 18 embryos) (Fig. 2, Movies 2A,B). This behavior, where NCCS migrate along ECs rather than crossing through EC cell streams, suggests that EC patterning may influence NCC positioning and ventral migration, and thereby contribute to the anterior/posterior patterning of the PNS.

To investigate whether ECs might direct NCCs to enter the intersomitic furrow alongside the anterior versus the posterior somite face, we analyzed numerous time-lapse reconstructions of migrating ECs and quantified their positions in static tissue sections. Figures 2, 3A, B and Movies 2A–B, 3A–D show ECs migrating dorsally in a stream within the intersomitic furrow until they reach the MSA. Although ECs turn in both anterior and posterior directions once they reach the MSA, the majority turns anteriorly such that they extend over the posterior half of the anteriorly adjacent somite (Fig. 3A,B and Movies 3A–D). Upon reaching the anterior/posterior midpoint of the somite, these ECs frequently turn ventrally again, exiting the MSA and diving into the somitic mesoderm where the fissure of von Ebner will emerge (Fig. 3B, arrows; Movie 3C), and contribute to the perineural vascular plexus (PNVP) (91). This migration pattern, wherein the bulk of ECs turn anteriorly within the MSA and then exit the MSA before extending over the anterior half somite, results in an asymmetric distribution of ECs, with the majority dorsal to the posterior-half somite. To validate this observation, the numbers of ECs bordering the anterior versus the posterior halves of each somite were quantified in whole embryos. As shown in Fig. 3C, there are almost twice the number of ECs bordering the posterior half of the somite as compared to the anterior half (n = 4 embryos). The schematic shown in Fig. 3D diagrams this basic migration pattern, also illustrated in time-lapse Movies 3B–D.

The EC migration pattern shown in Figure 3D likely generates a relatively continuous substratum positioned dorsally to the posterior half somite and extending ventrally into the intersomitic furrow (Fig. 3A, solid arrow). We posited that this “wall” of ECs covering the posterior half somite might block NCCs from penetrating the posterior surface, and thereby facilitate their migration into the intersomitic furrow. In support of this hypothesis, ECs within the MSA at positions directly above (dorsal to) the posterior half somite were consistently located on the ventral side of NCCs (arrows in Fig. 3E). In addition, in both static images of longitudinal explants (Fig. 3F), and in time lapse imaging of transverse slice explants through the posterior somite (Movie 3E; arrowhead), ECs appear to form a barrier above the posterior somite half, potentially preventing NCC ventral migration. In contrast, the relative absence of ECs above the anterior half (asterisks in Fig. 3F), may facilitate NCC penetration into the anterior somite (16, 92).

Although ECs are more uniformly distributed along the length of the MSA towards the end of NCC migration (St. 20–22), there are still gaps between them such that late migrating NCCs can still access the ganglion (asterisks in Fig. 3G). Movies 3F,G and Figures 3H–K show a live transverse slice through the anterior somite of a Stage 22 Tg(tie1:H2B-eYFP; syn1:eGFP) quail embryo including the developing DRG. In this transgenic line, endothelial cells (tie1+) express nuclear-localized eYFP and differentiated neurons (synapsin+) express cytoplasmic eGFP including motor neurons and neurons in the DRG core and ventral lateral region (93). Late NCCs are seen migrating ventrolaterally along ECs comprising the PNVP that surrounds the neural tube, which by now is somewhat continuous with the vasculature surrounding the DRG. These higher resolution images show the elongated morphology of a NCC as it migrates along a relatively continuous layer of ECs toward the DRG, and how this morphology changes when the NCC encounters a gap in the vasculature; it extends filopodia into the gap followed by a complete change in cell shape and alignment such that the cell body also begins squeezing through the gap toward the DRG. These time-lapse data strongly suggest that EC cell patterning influences NCC migratory behavior.

Neural crest cells track along the intersomitic vessel to colonize the sympathetic ganglia

Early NCCs that migrate ventrally within the intersomitic furrow are destined to colonize the sympathetic ganglia (69). The schematic in Figure 4A shows the developing intersomitic vessel projecting both ventrally and laterally to the dorsal/ventral and medial/lateral level of the presumptive SG where it intersects the wall of the postcardinal vein (PCV) (36). Movie 4 of a live transverse explant, and Figure 4B show that early-migrating NCCs continue to track along the ISV even at dorsal/ventral levels that position them beyond the ventral border of the somite. Similar to their behavior within the furrow, they move in a step-like fashion along the stream of ECs ventrolaterally toward their target next to the dorsal aorta. These NCCs remain on the lateral side of the ISV, and do not cross the EC stream, until they encounter a second, larger stream of ECs running in the anterior/posterior direction that form the wall of the PCV. After contacting the PCV, NCCs turn and migrate through the intersomitic stream to the presumptive location of the SG. The dashed line with arrowheads in Figure 4A shows this basic migration pattern. Figure 4C shows a similar transverse cryosection immunostained with HNK1 antibody to identify the location of the developing SG (compare HNK1 staining in Fig. 4C to NCC positioning in Fig. 4B and Movie 4). Movie 4 also shows how contact with a pioneer NCC already in the SG anlage may help recruit the more dorsal NCC to colonize the SG.

Neural crest contact with the perisomitic vessel may establish the ventral border of the DRG and induce the “back fill” colonization of the dorsal root ganglion

Using both Tg(tie1:H2B-eYFP) quail and immunostaining with the EC marker QH1, we observed a discrete population of ECs at St.12/13 that are consistently located at the motor axon exit point from the spinal cord (Fig. 5A). These ECs are in the perisomitic vessel (PSV) (11), also referred to as the longitudinal anastomosis (35). Their stereotyped localization positions them for contact with the earliest migrating NCCs that travel between the neural tube and the somite (6, 11, 35). The majority of this NCC cohort migrates to the sympathetic ganglia via an SDF-1/CXCR4 mediated chemotaxis, but a minority seed the future DRG (10, 13). Previous studies show that the first NCCs that colonize the DRG anlagen express the basic helix-loop-helix transcription factor Ngn2 (94) and act as a nucleation site around which later-migrating NCCs condense (13, 95). We noticed that these Ngn2+ NCCs are typically located at the ventromedial border of what will be the DRG and asked whether their cessation in migration at that point correlates spatially with the PSV. Analysis of static tissue sections shows that NCCs immediately adjacent to the neural tube are frequently Ngn2+, and based on their relative positions, likely contact PSV ECs (Fig. 5B,C). To investigate the dynamics between these two cells types we imaged their interaction in live tissue. Movie 5 shows how ventrally migrating NCCs begin to stack up dorsally on top of the PSV when they encounter the developing vessel. Figure 1A and Movie 1B also show how the more mature PSV spatially aligns with the ventral border of the DRG.

Figure 5
Endothelial cells associate with early neural crest cells that seed the dorsal root ganglion and gradually encapsulate the ganglion as it condenses. A–F) 16 μm transverse cryosections. Green nuclei correspond to ECs Tg(tie1:H2B-eYFP). ...

Endothelial cells encapsulate the nascent dorsal root ganglion and the emerging spinal nerves

As DRG development continues, ECs gradually circumscribe the DRG and by St. 23–25 encapsulate the entire ganglion and the emerging spinal nerves (Figure 5 D–G; Movie 1A). Although cell-to-cell cohesion likely functions in constraining NCCs to the condensing DRG, our time-lapse imaging reveals continual interactions between NCCs and these border ECs that also appear to constrain NCCs (Movie 6). NCCs that initiate movements out of the anlage often reverse this motion or retract their filopodia following contact with a border EC. Movie 1A and Figure 5G also show how the emerging spinal nerves are tightly associated with a network of vasculature.

Proximity to endothelial cells is associated with mitotic activity of dorsal root ganglia progenitor cells

As the DRG matures, a fundamental segregation manifests in which differentiating neurons are confined to the inner core, while mitotically active progenitor cells colonize the perimeter (5, 13, 96). We have shown previously that the DRG perimeter is comprised of two molecularly distinct progenitor zones: cells within the dorsal pole and along the medial and dorsolateral perimeter express PAX3 and SOX10, while those along the ventrolateral perimeter are SOX10+/PAX3 (13). We have also shown that the PAX3+ subset goes on to specifically give rise to the majority of TRKA+ pain and temperature responsive sensory neurons, while the SOX10+ cells stay in the cell cycle and give rise to glial cells (13). Here we show an asymmetry in the pattern of EC condensation around the DRG perimeter that is spatially and temporally aligned with the PAX3+ zone, with numerous ECs tightly apposed to PAX3+ progenitors (Fig. 6A,B). In contrast, there are relatively few ECs along the PAX3 zone that comprises the lateral and ventral perimeter of the DRG. Since sensory neurogenesis precedes gliogenesis (13, 97, 98), we asked whether mitotically-active progenitors within the DRG perimeter were more likely to be in contact with ECs, than were non-proliferating cells within the perimeter. That analysis (Fig. 6C) reveals that in fact progenitors in M phase are more likely to be in contact with ECs as compared to non-proliferating progenitor cells. During the peak of proliferation (St. 26/27), an average of 80% of cycling pH3+ progenitors were in contact with ECs (n = 3 embryos, 246 cells). Interestingly, at younger ages (St. 21/22), while cycling cells were still more likely to be in contact with ECs compared to non-cycling cells, the difference was less dramatic suggesting that other, non EC-associated mitogenic sources drive progenitor cell proliferation at earlier time points. During the peak period of PAX3+ progenitor cell proliferation, a dense layer of laminin (Fig. 6D) is deposited between the DRG progenitors and the apposed ECs. Since the extracellular matrix is a repository of growth factors and mitogens including fibroblast growth factors (FGFs), insulin-like growth factors (IGFs) hepatocyte growth factors (HGFs), transforming growth factors (TGFs), platelet-derived growth factors, and vascular endothelial growth factor (VEGF), it may act as a mediator of communication between these DRG progenitors and ECs (99).


This is the first study to image in real time the intercellular interactions between migrating neural crest cells and endothelial cells during development of the PNS. In this study, we have exploited the power of live imaging technologies in transgenic quail that express both neural and endothelial cell fluorescent markers to image neural crest cells migrating in their in vivo environment and interacting with neighboring cell types, in this case, endothelial cells. Similar to other live imaging studies that have revealed novel intercellular and molecular interactions not previously discerned from analyses of static tissue (21, 22, 26, 100), this work reveals novel, hitherto undescribed dynamic intercellular interactions that implicate key functions for ECs during NCC migration and condensation as they form dorsal root and sympathetic ganglia in vivo. Our data demonstrate that NCCs interact with ECs at key junctures during their migration and differentiation including: (1) as NCCs migrate along the MSA; (2) as they enter and migrate through the intersomitic furrow; (3) as they segregate to form discrete sympathetic ganglia (4) when the first NCCs that seed the DRG stop at the future ventral-most axis of the DRG anlage; (5) during formation of DRG boundaries; (6) as the developing spinal nerves become encased in vasculature; (7) during the proliferation of perimeter-localized DRG progenitor cells. With a new awareness of the interactive relationship between endothelial cells and neural crest cells, the goal will be to identify the molecular mechanisms that orchestrate and mediate these behaviors. In summary, the data presented here underscore the power of the Tg tie1 quail as a novel and powerful tool for studying EC migration patterns and demonstrate that EC movements, in particular during formation of the intersomitic vessels and their asymmetric colonization above the anterior and posterior halves of the somite, are more complex than have been previously reported (101, 102).

Analysis of our live imaging data supports a model in which ECs both direct and constrain the migratory behavior and path of NCCs during development of the PNS. While it is conceivable that both NCCs and ECs could be responding to the same guidance cues in shared locations, the fact that NCCs alter their migratory route following direct contact with ECs suggests their behavior is influenced by this contact, rather than by some other environmental cue. Depending on the location, ECs provide either a permissive substrate facilitating NCC migration, or they appear to constrain movement, preventing the emigration of nascent DRG cells from the anlage and restraining further ventral migration into the developing SG. This last behavior was described in an previous study (22) in which we showed that at early stages, NCCs in the DRG and SG anlagen could exchange locales, but by St. 17 a “boundary” was established that clearly inhibited NCCs from entering the more dorsal or ventral ganglia. Here we show that this boundary correlates temporally and spatially with the perisomitic vessel, or longitudinal anastomosis (11, 35).

Prior observations of the close apposition of migrating NCCs and the ISVs have posited that they independently respond to shared cues in the intersomitic furrow (9, 11, 35, 36). One study demonstrated that DRG form in their normal locations in the zebrafish mutant, cloche, in which some blood vessels, including the ISVs are eliminated, suggesting that DRG formation is independent of these vessels (35, 102). However, the major anterior/posterior vessels form normally in cloche embryos, including the dorsal aorta, and numerous individual ECs in the location of the ISVs are still present (35). Moreover, since early NCCs that migrate within the intersomitic furrow colonize the SG rather than the DRG, we would predict that SG, rather than DRG, formation would be impacted by loss of the intersomitic vessels. The fibronectin rich extracellular matrix associated with the ISV likely promotes NCC adhesion to ECs and migration along them, such that early NCCs migrate ventrally between the ISV and the somite (36), rather than penetrating the anterior half somite precociously. Multiple previous studies have shown a spatiotemporal correlation between the presence of fibronectin and NCC migration, as well as the cessation of NCC movement with the local disappearance of fibronectin. In vitro studies have also shown fibronectin and cultured ECs to be an excellent substrate for NCC adhesion and motility (7, 36, 103, 104).

Ephrins are important signaling molecules mediating the development of both the neural crest and endothelial lineages (15, 105). A previous study demonstrated that avian ECs that are positioned above (dorsal to) the posterior half somite and extending into the entrance to the intersomitic furrow express EphrinB2 (16). These are the same ECs that our live time-lapses document interacting with NCCs as they leave the MSA to enter the furrow and that appear to provide a substrate for NCCs as they migrate ventrally within the furrow (Movies 2A,B). Although early expression of ephrin ligands in the avian dermomyotome prevents NCCs from entering the dorsolateral pathway, later expression of EphrinB2 within the dermomyotome promotes the migration of late NCCs (melanoblasts) into this same pathway (106). Thus similar to the dual role that ephrins play in the dorsolateral pathway, ephrins may also play opposing roles in relation to the ventral pathway; although EphrinB1 expression within the avian posterior half-sclerotome blocks the ventral migration of NCCs (16), EphrinB2 expression by ECs may actually recruit NCCs out of the MSA and stimulate their procession into the intersomitic furrow. In support of this model, the binding of EphrinB2 ligands by NCC EphB receptors has been shown to promote NCC motility by increasing cell adhesion (106). Alternatively, since siRNA knockdown of EphrinB2 causes reduced filopodial activity in ISV endothelial tip cells, EphrinB2 could play a less direct role in promoting NCC migration via simply promoting EC/NCC contact (101). The ultimate test of a role for ECs in patterning NCC migration will require the elimination of ECs in a spatially, temporally and cell type-specific controlled manner, as any crude systematic elimination can induce indirect effects.

This study is not the first to report interactions between ECs and NCCs that influence PNS development. Recent in vitro studies demonstrate that co-culturing of ECs and vascular smooth muscle cells with human embryonic stem cell derived-NCCs induced their differentiation into sensory and autonomic neurons (107). Interestingly, many vascular smooth muscle cells are themselves derived from the neural crest (108112) pointing to the multiple nodes of interactions between these two lineages. Previous work in chick embryos has shown that dorsal aorta-derived BMPs are required for differentiation of sympathetic neurons (17, 113) and in zebrafish it has been recently reported that PDGFR-mediated maturation of vascular mural cells is required for full differentiation of a sympathetic, noradrenergic fate (114). The cardiac crest is well known to be integrally involved in development of the cardiovascular system coordinating with ECs and smooth muscle cells to generate the outflow tract and cardiac cushions via SEMA3C and NRP1 (115). Similarly the cephalic crest also plays a key role in mediating the vascularization of the facial cartilage by producing a vital source of VEGF (116) and ECs are essential for promoting the migration and survival of NCCs (117). Thus the multistep differentiation process of these two embryologically distinct lineages are highly interdependent and converge spatially and temporally to regulate each other’s development.

In the PNS, our understanding of how these two embryologically distinct systems first interact and the significance of their interactions as they form is sparse, nor do we understand the mechanisms that lead to peripheral neuropathy should this neurovascular cross talk break down. By live imaging the development of the vascular and peripheral nervous systems with cell type-specific markers, we present here the first evidence for direct interactions between these two systems in real time. Our studies demonstrate that ECs and NCCs interact extensively during formation of the sensory and sympathetic ganglia, and suggest that ECs provide critical guidance cues, either positive or negative depending on their location, to migrating NCCs. With further elucidation of the genes and pathways that mediate communication between NCCs and ECs, a more comprehensive understanding of the interdependency of these two systems both during development and disease will be possible.


  • Live in vivo imaging of endothelial and neural crest cells during PNS development
  • We report extensive dynamic interactions between neural crest and endothelial cells
  • Endothelial cells promote or restrict neural crest migration according to location
  • These interactions likely contribute to the patterning of the peripheral nervous system

Supplementary Material


Movie 1A. By the end of NCC migration the peripheral ganglia and spinal nerves are surrounded by a vascular network. St. 23 quail. Fly thru Movie 1A shows a medial to lateral progression through the mid-trunk (between the wing bud and hind limb), beginning at the midsagittal plane that includes the embryonic spinal cord. The embryo was immunostained using primary antibodies that recognize NCCs and NCC derivatives (HNK1), and ECs and EC derived vasculature (QH1), cleared, and then cut in half along the sagittal midline (see methods). DRG, dorsal root ganglia; ISV, intersomitic vessel; PNVP, perineural vascular plexus; PSV, perisomitic vessel; SG, sympathetic ganglia. (Scale bar: 110 μm)

Movie 1B. The developing PNS and vasculature are tightly juxtaposed. St. 18 quail. Rotational Movie 1B shows a reconstructed view of the sagittal half of the mid-embryonic trunk. The embryo was immunostained with primary antibodies that recognize NCCs and NCC derivatives (HNK1), and ECs and EC-derived vasculature (QH1), cleared, and then cut in half along the sagittal midline (see methods). DRG, dorsal root ganglia; ISV, intersomitic vessel; PNVP, perineural vascular plexus; SG, sympathetic ganglia. (Scale bar: 120 μm)

Movie 1C. NCCs are directly apposed to ECs as they enter the intersomitic furrow. St. 15/16 quail. Fly thru Movie 1C shows a dorsal to ventral progression through the dorsal half of the mid-embryonic trunk (somites 16–21). The embryo was immunostained using primary antibodies that recognize NCCs and NCC derivatives (HNK1), and ECs and EC-derived vasculature (QH1), cleared, and then imaged whole mount with the dorsal surface laid down on a cover glass. At this stage, the MSA is densely populated with NCCs with a few entering the intersomitic furrow in tight juxtaposition to ECs comprising the intersomitic vessel. The anterior half of the somite also shows some HNK1 immunoreactivity. DRG, dorsal root ganglia; ISV, intersomitic vessel; MSA, migration staging area; NCCs, neural crest cells; NT, neural tube. (Scale bar: 130 μm)

Movies 2A and 2B. NCCs interact extensively with ECs as they migrate ventrally through the intersomitic furrow. Multispectral time-lapse confocal microscopy of a live, whole mount St. 16 Tg (tie1:H2B-eYFP) quail embryo viewed from the dorsolateral aspect. Axial level corresponds to the mid-trunk. The neural tube was electroporated with a ChFP expression plasmid at St. 11/12 to label the premigratory crest. NCCs track along the posterior surface of ECs as they migrate ventrally within the intersomitic furrow, making numerous filopodial contacts with ECs during their ventral migration. 2A zooms in on a single somite, while 2B shows more than three somites. 2A time-lapse duration = 15 hrs, 24 min. 2B time-lapse duration = 3 hr, 20 min. DRG, dorsal root ganglia; ECs, endothelial cells; ISV, intersomitic vessel; MSA, migration staging area; NCCs, neural crest cells; PCV, postcardinal vein. (Scale bar: 2A, 45 μm; 2B, 36 μm)

Movie 2C. NCCs make extensive contact with both ECs and the anterior face of the somite. St. 14–15 whole mount Tg (tie1:H2B-eYFP) quail embryos viewed from the dorsal aspect, somites 13–15. The neural tube was electroporated with a ChFP expression plasmid at St. 11/12 to label NCCs. In the left panel fluorescent time-lapse images are overlaid with DIC, allowing for visualization of the somite as well as migrating NCCs and ECs. NCCs enter the intersomitic furrow between the developing ISV and the anterior face of the somite, making extensive contact with both. The right panel shows DIC alone in grayscale to provide an enhanced perspective of the somites and the size of the intersomitic furrow. Time-lapse duration = 7 hours, 44 min. ECs, endothelial cells; NCCs, neural crest cells. (Scale = 0.32 μm per pixel).

Movies 3A–3D. EC migration patterns demarcate NCC boundaries. St. 15 whole mount Tg (tie1:H2B-eYFP) quail embryos viewed from the dorso-lateral aspect. Time-lapse imaging shows that ECs migrate in stereotyped patterns as they form the embryonic vasculature. ECs migrate dorsally within the intersomitic furrow until they reach the migratory staging area where they primarily turn in the anterior direction such that they asymmetrically cover the posterior half of the somite. Movie 3A zooms in on somites 4 and 5 and shows ECs migrating within a single intersomitic furrow. Movie 3B shows somites 3, 4 and 5 as well as 3 intersomitic furrows, and Movie 3C shows somites 3–11 and 9 intersomitic furrows. Movie 3D shows somites 3–16 with DIC in the lower panel. 3A time-lapse duration = 3 hrs, 39 min. 3B,C time-lapse duration = 16 hrs, 4 min. 3D time-lapse duration = 13 hours, 59 min. ECs, endothelial cells; ISV, intersomitic vessel. (Scale = 0.64 μm per pixel)

Movie 3E. EC patterning may help prevent NCCs from entering the posterior somite. Time-lapse confocal microscopy of a live, 200-μm transverse slice explant through the posterior half of a somite from the mid-trunk of a St. 17 Tg (tie1:H2B-eYFP) quail embryo shows contralaterally migrating NCCs within the MSA bumping up against ECs that appear to block them from further ventral migration. Time-lapse duration = 6 hrs, 35 min. ECs, endothelial cells; NCCs, neural crest cells. (Scale bar: 24 μm)

Movies 3F,G. A NCC changes its migration direction and morphology upon encountering a gap in the EC stream. St. 22 Tg (tie1:H2B-eYFP; syn1:eGFP) quail embryo in which the neural tube was electroporated with a ChFP expression plasmid at St. 11/12 to label the premigratory crest. In this transgenic line endothelial cells (tie1+) express nuclear localized eYFP, and differentiated neurons (synapsin+), including motor neurons and neurons in the core and ventral lateral region of the DRG, express cytoplasmic eGFP. Time-lapse confocal microscopy of a live, 200-μm transverse slice through the anterior somite shows the elongated morphology of a NCC (arrowhead) as it tracks in the ventrolateral direction along a relatively continuous layer of ECs, and how this morphology and trajectory change when the NCC encounters a gap in the vasculature. 3F,G time-lapse duration = 4 hours, 30 min. DRG, dorsal root ganglion. (Scale bar: 3F, 30 μm; 3G, 20 μm)

Movie 4. ECs appear to direct early migrating NCCs toward the sympathetic ganglia. St. 17 Tg (tie1:H2B-eYFP) quail in which the neural tube was electroporated with a ChFP expression plasmid to label NCCs. Time-lapse confocal microscopy of a live, 200-μm transverse slice explant. A developing intersomitic vessel is shown with NCCs tracking along ECs as they migrate ventrolaterally until they reach the PCV, where they turn and migrate medially to colonize the SG. Time-lapse duration = 2 hrs, 25 min. DA, dorsal aorta; ECs, endothelial cell; ISV, intersomitic vessel; NCCs, neural crest cells; PCV, postcardinal vein; SG, sympathetic ganglia. (Scale bar: 40 μm)

Movie 5. NCCs that encounter the perisomitic vessel stop their ventral migration and colonize the DRG. St. 17 Tg (tie1:H2B-eYFP) quail in which the neural tube was electroporated with a ChFP expression plasmid to label NCCs. Time-lapse confocal microscopy of a live, 200-μm transverse slice explant shows how NCCs that have migrated as far ventrally as the perisomitic vessel, appear to stop their ventral migration upon contact with the vessel, which thereby defines the ventral-most point of the DRG anlage. Time-lapse duration = 6 hrs, 40 min. NCCs, neural crest cells; PSV, perisomitic vessel. (Scale bar: 24 μm)

Movie 6. ECs may constrain NCCs and prevent their migration away from the DRG. Movie 6 shows the contralateral half of a 200 μm transverse slice explant from a St. 18 Tg (tie1:H2B-eYFP) quail in which the neural tube was electroporated with a ChFP expression plasmid to label NCCs. Since only contralaterally migrating NCCs are labeled on the non-electroporated half, viewing this side aids in the visualization of individual NCCs and filopodial dynamics. Time-lapse confocal microscopy shows how ECs begin to encapsulate the nascent DRG and appear to constrain NCCs within the condensing anlage. Time-lapse duration = 4 hrs, 55 min. DRG, dorsal root ganglion; ECs, endothelial cells; NCCs, neural crest cells; PSV, perisomitic vessel. (Scale bar: 24 μm)




We thank Marta Chaverra for technical support. This work was supported by NIH NINDS 35714 to F.L.


Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.


1. Lallier TE, Bronner-Fraser M. A spatial and temporal analysis of dorsal root and sympathetic ganglion formation in the avian embryo. Dev Biol. 1988;127(1):99–112. Epub 1988/05/01. doi: 0012-1606(88)90192-3 [pii] [PubMed]
2. Le Douarin NM, Kalcheim C. The Neural Crest. 2. Vol. 1999. Cambridge University Press; 1999. Nov 28, p. 445.
3. Tosney KW. The early migration of neural crest cells in the trunk region of the avian embryo: an electron microscopic study. Dev Biol. 1978;62(2):317–33. Epub 1978/02/01. doi: 0012-1606(78)90219-1 [pii] [PubMed]
4. Weston JA. The migration and differentiation of neural crest cells. Adv Morphog. 1970;8:41–114. Epub 1970/01/01. [PubMed]
5. George L, Chaverra M, Todd V, Lansford R, Lefcort F. Nociceptive sensory neurons derive from contralaterally migrating, fate-restricted neural crest cells. Nat Neurosci. 2007;10(10):1287–93. Epub 2007/09/11. doi: nn1962 [pii] 10.1038/nn1962. [PubMed]
6. Loring JF, Erickson CA. Neural crest cell migratory pathways in the trunk of the chick embryo. Dev Biol. 1987;121(1):220–36. Epub 1987/05/01. doi: 0012-1606(87)90154-0 [pii] [PubMed]
7. Thiery JP, Duband JL, Delouvee A. Pathways and mechanisms of avian trunk neural crest cell migration and localization. Dev Biol. 1982;93(2):324–43. [PubMed]
8. Teillet MA, Kalcheim C, Le Douarin NM. Formation of the dorsal root ganglia in the avian embryo: segmental origin and migratory behavior of neural crest progenitor cells. Dev Biol. 1987;120(2):329–47. [PubMed]
9. Erickson CA. Control of neural crest cell dispersion in the trunk of the avian embryo. Dev Biol. 1985;111(1):138–57. Epub 1985/09/01. doi: 0012-1606(85)90442-7 [pii] [PubMed]
10. Kasemeier-Kulesa JC, McLennan R, Romine MH, Kulesa PM, Lefcort F. CXCR4 controls ventral migration of sympathetic precursor cells. J Neurosci. 2010;30(39):13078–88. Epub 2010/10/01. doi: 30/39/13078 [pii] 10.1523/JNEUROSCI.0892-10.2010. [PubMed]
11. Schwarz Q, Maden CH, Vieira JM, Ruhrberg C. Neuropilin 1 signaling guides neural crest cells to coordinate pathway choice with cell specification. Proc Natl Acad Sci U S A. 2009;106(15):6164–9. Epub 2009/03/28. doi: 0811521106 [pii] 10.1073/pnas.0811521106. [PubMed]
12. Bronner-Fraser M. Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1. Dev Biol. 1986;115(1):44–55. [PubMed]
13. George L, Kasemeier-Kulesa J, Nelson BR, Koyano-Nakagawa N, Lefcort F. Patterned assembly and neurogenesis in the chick dorsal root ganglion. J Comp Neurol. 2010;518(4):405–22. doi: 10.1002/cne.22248. Epub 2009/12/18. [PMC free article] [PubMed] [Cross Ref]
14. Weston JA. Sequential segregation and fate of developmentally restricted intermediate cell populations in the neural crest lineage. Curr Top Dev Biol. 1991;25:133–53. [PubMed]
15. Gammill LS, Gonzalez C, Gu C, Bronner-Fraser M. Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling. Development. 2006;133(1):99–106. Epub 2005/12/02. doi: dev.02187 [pii] 10.1242/dev.02187. [PubMed]
16. Krull CE, Lansford R, Gale NW, Collazo A, Marcelle C, Yancopoulos GD, Fraser SE, Bronner-Fraser M. Interactions of Eph-related receptors and ligands confer rostrocaudal pattern to trunk neural crest migration. Curr Biol. 1997;7(8):571–80. [PubMed]
17. Saito D, Takase Y, Murai H, Takahashi Y. The dorsal aorta initiates a molecular cascade that instructs sympatho-adrenal specification. Science. 2012;336(6088):1578–81. doi: 10.1126/science.1222369. [PubMed] [Cross Ref]
18. Clay MR, Halloran MC. Control of neural crest cell behavior and migration: Insights from live imaging. Cell Adh Migr. 2010;4(4):586–94. Epub 2010/07/31. doi: 12902 [pii] 10.4161/cam.4.4.12902. [PMC free article] [PubMed]
19. Clay MR, Halloran MC. Regulation of cell adhesions and motility during initiation of neural crest migration. Curr Opin Neurobiol. 2011;21(1):17–22. Epub 2010/10/26. doi: S0959-4388(10)00183-2 [pii] 10.1016/j.conb.2010.09.013. [PMC free article] [PubMed]
20. Jesuthasan S. Contact inhibition/collapse and pathfinding of neural crest cells in the zebrafish trunk. Development. 1996;122(1):381–9. Epub 1996/01/01. [PubMed]
21. Kasemeier-Kulesa JC, Bradley R, Pasquale EB, Lefcort F, Kulesa PM. Eph/ephrins and N-cadherin coordinate to control the pattern of sympathetic ganglia. Development. 2006;133(24):4839–47. Epub 2006/11/17. doi: dev.02662 [pii] 10.1242/dev.02662. [PubMed]
22. Kasemeier-Kulesa JC, Kulesa PM, Lefcort F. Imaging neural crest cell dynamics during formation of dorsal root ganglia and sympathetic ganglia. Development. 2005;132(2):235–45. Epub 2004/12/14. doi: dev.01553 [pii] 10.1242/dev.01553. [PubMed]
23. Krull CE, Collazo A, Fraser SE, Bronner-Fraser M. Segmental migration of trunk neural crest: time-lapse analysis reveals a role for PNA-binding molecules. Development. 1995;121(11):3733–43. Epub 1995/11/01. [PubMed]
24. Kulesa PM, Fraser SE. Neural crest cell dynamics revealed by time-lapse video microscopy of whole embryo chick explant cultures. Dev Biol. 1998;204(2):327–44. Epub 1999/01/12. doi: S0012-1606(98)99082-0 [pii] 10.1006/dbio.1998.9082. [PubMed]
25. Kulesa PM, Lefcort F, Kasemeier-Kulesa JC. The migration of autonomic precursor cells in the embryo. Autonomic neuroscience: basic & clinical. 2009;151(1):3–9. doi: 10.1016/j.autneu.2009.08.013. [PubMed] [Cross Ref]
26. Kulesa PM, Morrison JA, Bailey CM. The neural crest and cancer: a developmental spin on melanoma. Cells Tissues Organs. 2013;198(1):12–21. doi: 10.1159/000348418. [PMC free article] [PubMed] [Cross Ref]
27. Kulesa PM, Teddy JM, Stark DA, Smith SE, McLennan R. Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR. Dev Biol. 2008;316(2):275–87. Epub 2008/03/11. doi: S0012-1606(08)00064-X [pii] 10.1016/j.ydbio.2008.01.029. [PMC free article] [PubMed]
28. Langenberg T, Kahana A, Wszalek JA, Halloran MC. The eye organizes neural crest cell migration. Dev Dyn. 2008;237(6):1645–52. doi: 10.1002/dvdy.21577. Epub 2008/05/24. [PMC free article] [PubMed] [Cross Ref]
29. Teddy JM, Kulesa PM. In vivo evidence for short- and long-range cell communication in cranial neural crest cells. Development. 2004;131(24):6141–51. doi: 10.1242/dev.01534. [PubMed] [Cross Ref]
30. Young HM, Bergner AJ, Anderson RB, Enomoto H, Milbrandt J, Newgreen DF, Whitington PM. Dynamics of neural crest-derived cell migration in the embryonic mouse gut. Dev Biol. 2004;270(2):455–73. Epub 2004/06/09. doi: 10.1016/j.ydbio.2004.03.015 S0012160604002027 [pii] [PubMed]
31. Druckenbrod NR, Epstein ML. The pattern of neural crest advance in the cecum and colon. Dev Biol. 2005;287(1):125–33. doi: 10.1016/j.ydbio.2005.08.040. [PubMed] [Cross Ref]
32. Druckenbrod NR, Epstein ML. Behavior of enteric neural crest-derived cells varies with respect to the migratory wavefront. Dev Dyn. 2007;236(1):84–92. doi: 10.1002/dvdy.20974. [PubMed] [Cross Ref]
33. Kalcheim C, Goldstein RS. Segmentation of sensory and sympathetic ganglia: interactions between neural crest and somite cells. J Physiol (Paris) 1991;85(3):110–6. [PubMed]
34. Newgreen DF, Powell ME, Moser B. Spatiotemporal changes in HNK-1/L2 glycoconjugates on avian embryo somite and neural crest cells. Dev Biol. 1990;139(1):100–20. [PubMed]
35. Miller LC, Freter S, Liu F, Taylor JS, Patient R, Begbie J. Separating early sensory neuron and blood vessel patterning. Dev Dyn. 2010;239(12):3297–302. doi: 10.1002/dvdy.22464. [PubMed] [Cross Ref]
36. Spence SG, Poole TJ. Developing blood vessels and associated extracellular matrix as substrates for neural crest migration in Japanese quail, Coturnix coturnix japonica. Int J Dev Biol. 1994;38(1):85–98. [PubMed]
37. Nagy N, Mwizerwa O, Yaniv K, Carmel L, Pieretti-Vanmarcke R, Weinstein BM, Goldstein AM. Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling. Dev Biol. 2009;330(2):263–72. doi: 10.1016/j.ydbio.2009.03.025. [PMC free article] [PubMed] [Cross Ref]
38. Delalande JM, Natarajan D, Vernay B, Finlay M, Ruhrberg C, Thapar N, Burns AJ. Vascularisation is not necessary for gut colonisation by enteric neural crest cells. Dev Biol. 2014;385(2):220–9. doi: 10.1016/j.ydbio.2013.11.007. [PMC free article] [PubMed] [Cross Ref]
39. Coventry S, Yost C, Palmiter RD, Kapur RP. Migration of ganglion cell precursors in the ileoceca of normal and lethal spotted embryos, a murine model for Hirschsprung disease. Lab Invest. 1994;71(1):82–93. [PubMed]
40. Landman KA, Fernando AE, Zhang D, Newgreen DF. Building stable chains with motile agents: Insights into the morphology of enteric neural crest cell migration. J Theor Biol. 2011;276(1):250–68. doi: 10.1016/j.jtbi.2011.01.043. [PubMed] [Cross Ref]
41. Bautch VL, James JM. Neurovascular development: The beginning of a beautiful friendship. Cell Adh Migr. 2009;3(2):199–204. [PMC free article] [PubMed]
42. Carmeliet P. Blood vessels and nerves: common signals, pathways and diseases. Nat Rev Genet. 2003;4(9):710–20. doi: 10.1038/nrg1158. [PubMed] [Cross Ref]
43. Eichmann A, Thomas JL. Molecular parallels between neural and vascular development. Cold Spring Harb Perspect Med. 2013;3(1):a006551. doi: 10.1101/cshperspect.a006551. [PMC free article] [PubMed] [Cross Ref]
44. Gelfand MV, Hong S, Gu C. Guidance from above: common cues direct distinct signaling outcomes in vascular and neural patterning. Trends Cell Biol. 2009;19(3):99–110. doi: 10.1016/j.tcb.2009.01.001. [PMC free article] [PubMed] [Cross Ref]
45. Bates D, Taylor GI, Newgreen DF. The pattern of neurovascular development in the forelimb of the quail embryo. Dev Biol. 2002;249(2):300–20. [PubMed]
46. Taylor GI, Gianoutsos MP, Morris SF. The neurovascular territories of the skin and muscles: anatomic study and clinical implications. Plast Reconstr Surg. 1994;94(1):1–36. [PubMed]
47. Quaegebeur A, Lange C, Carmeliet P. The neurovascular link in health and disease: molecular mechanisms and therapeutic implications. Neuron. 2011;71(3):406–24. doi: 10.1016/j.neuron.2011.07.013. [PubMed] [Cross Ref]
48. Vesalius A. De humani corporis fabrica libri septem1543 [PubMed]
49. Erskine L, Reijntjes S, Pratt T, Denti L, Schwarz Q, Vieira JM, Alakakone B, Shewan D, Ruhrberg C. VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron. 2011;70(5):951–65. doi: 10.1016/j.neuron.2011.02.052. [PMC free article] [PubMed] [Cross Ref]
50. Suchting S, Heal P, Tahtis K, Stewart LM, Bicknell R. Soluble Robo4 receptor inhibits in vivo angiogenesis and endothelial cell migration. FASEB J. 2005;19(1):121–3. doi: 10.1096/fj.04-1991fje. [PubMed] [Cross Ref]
51. Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J Clin Invest. 2008;118(6):2062–75. doi: 10.1172/JCI32832. [PubMed] [Cross Ref]
52. Burnstock G, Ralevic V. New insights into the local regulation of blood flow by perivascular nerves and endothelium. Br J Plast Surg. 1994;47(8):527–43. [PubMed]
53. Martin P, Lewis J. Origins of the neurovascular bundle: interactions between developing nerves and blood vessels in embryonic chick skin. Int J Dev Biol. 1989;33(3):379–87. [PubMed]
54. Mukouyama YS, Shin D, Britsch S, Taniguchi M, Anderson DJ. Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell. 2002;109(6):693–705. [PubMed]
55. Bovetti S, Bovolin P, Perroteau I, Puche AC. Subventricular zone-derived neuroblast migration to the olfactory bulb is modulated by matrix remodelling. Eur J Neurosci. 2007;25(7):2021–33. doi: 10.1111/j.1460-9568.2007.05441.x. [PubMed] [Cross Ref]
56. Saghatelyan A. Role of blood vessels in the neuronal migration. Semin Cell Dev Biol. 2009;20(6):744–50. doi: 10.1016/j.semcdb.2009.04.006. [PubMed] [Cross Ref]
57. Snapyan M, Lemasson M, Brill MS, Blais M, Massouh M, Ninkovic J, Gravel C, Berthod F, Gotz M, Barker PA, Parent A, Saghatelyan A. Vasculature guides migrating neuronal precursors in the adult mammalian forebrain via brain-derived neurotrophic factor signaling. J Neurosci. 2009;29(13):4172–88. doi: 10.1523/JNEUROSCI.4956-08.2009. [PubMed] [Cross Ref]
58. Goldman SA, Chen Z. Perivascular instruction of cell genesis and fate in the adult brain. Nat Neurosci. 2011;14(11):1382–9. doi: 10.1038/nn.2963. [PMC free article] [PubMed] [Cross Ref]
59. Haigh JJ, Ema M, Haigh K, Gertsenstein M, Greer P, Rossant J, Nagy A, Wagner EF. Activated Fps/Fes partially rescues the in vivo developmental potential of Flk1-deficient vascular progenitor cells. Blood. 2004;103(3):912–20. doi: 10.1182/blood-2003-07-2343. [PubMed] [Cross Ref]
60. Makita T, Sucov HM, Gariepy CE, Yanagisawa M, Ginty DD. Endothelins are vascular-derived axonal guidance cues for developing sympathetic neurons. Nature. 2008;452(7188):759–63. doi: 10.1038/nature06859. [PMC free article] [PubMed] [Cross Ref]
61. Mukouyama YS, Gerber HP, Ferrara N, Gu C, Anderson DJ. Peripheral nerve-derived VEGF promotes arterial differentiation via neuropilin 1-mediated positive feedback. Development. 2005;132(5):941–52. doi: 10.1242/dev.01675. [PubMed] [Cross Ref]
62. Sondell M, Lundborg G, Kanje M. Vascular endothelial growth factor has neurotrophic activity and stimulates axonal outgrowth, enhancing cell survival and Schwann cell proliferation in the peripheral nervous system. J Neurosci. 1999;19(14):5731–40. [PubMed]
63. Vieira JM, Schwarz Q, Ruhrberg C. Role of the neuropilin ligands VEGF164 and SEMA3A in neuronal and vascular patterning in the mouse. Novartis Found Symp. 2007;283:230–5. discussion 5-41. [PMC free article] [PubMed]
64. Zacchigna S, Lambrechts D, Carmeliet P. Neurovascular signalling defects in neurodegeneration. Nat Rev Neurosci. 2008;9(3):169–81. doi: 10.1038/nrn2336. [PubMed] [Cross Ref]
65. Hashimoto T, Zhang XM, Chen BY, Yang XJ. VEGF activates divergent intracellular signaling components to regulate retinal progenitor cell proliferation and neuronal differentiation. Development. 2006;133(11):2201–10. doi: 10.1242/dev.02385. [PubMed] [Cross Ref]
66. Bouvree K, Larrivee B, Lv X, Yuan L, DeLafarge B, Freitas C, Mathivet T, Breant C, Tessier-Lavigne M, Bikfalvi A, Eichmann A, Pardanaud L. Netrin-1 inhibits sprouting angiogenesis in developing avian embryos. Dev Biol. 2008;318(1):172–83. doi: 10.1016/j.ydbio.2008.03.023. [PubMed] [Cross Ref]
67. Fujita M, Cha YR, Pham VN, Sakurai A, Roman BL, Gutkind JS, Weinstein BM. Assembly and patterning of the vascular network of the vertebrate hindbrain. Development. 2011;138(9):1705–15. doi: 10.1242/dev.058776. [PubMed] [Cross Ref]
68. Gu C, Yoshida Y, Livet J, Reimert DV, Mann F, Merte J, Henderson CE, Jessell TM, Kolodkin AL, Ginty DD. Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins. Science. 2005;307(5707):265–8. doi: 10.1126/science.1105416. [PubMed] [Cross Ref]
69. Herzog Y, Kalcheim C, Kahane N, Reshef R, Neufeld G. Differential expression of neuropilin-1 and neuropilin-2 in arteries and veins. Mech Dev. 2001;109(1):115–9. [PubMed]
70. Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nat Neurosci. 2006;9(3):340–8. doi: 10.1038/nn1646. [PubMed] [Cross Ref]
71. Pan Q, Chathery Y, Wu Y, Rathore N, Tong RK, Peale F, Bagri A, Tessier-Lavigne M, Koch AW, Watts RJ. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting. J Biol Chem. 2007;282(33):24049–56. doi: 10.1074/jbc.M703554200. [PubMed] [Cross Ref]
72. Suchting S, Freitas C, le Noble F, Benedito R, Breant C, Duarte A, Eichmann A. The Notch ligand Delta-like 4 negatively regulates endothelial tip cell formation and vessel branching. Proc Natl Acad Sci U S A. 2007;104(9):3225–30. doi: 10.1073/pnas.0611177104. [PubMed] [Cross Ref]
73. Weinstein BM. Vessels and nerves: marching to the same tune. Cell. 2005;120(3):299–302. doi: 10.1016/j.cell.2005.01.010. [PubMed] [Cross Ref]
74. Shen Q, Goderie SK, Jin L, Karanth N, Sun Y, Abramova N, Vincent P, Pumiglia K, Temple S. Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells. Science. 2004;304(5675):1338–40. doi: 10.1126/science.1095505. [PubMed] [Cross Ref]
75. Sato Y, Poynter G, Huss D, Filla MB, Czirok A, Rongish BJ, Little CD, Fraser SE, Lansford R. Dynamic analysis of vascular morphogenesis using transgenic quail embryos. PLoS One. 2010;5(9):e12674. doi: 10.1371/journal.pone.0012674. [PMC free article] [PubMed] [Cross Ref]
76. Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo. 1951. Dev Dyn. 1992;195(4):231–72. doi: 10.1002/aja.1001950404. Epub 1992/12/01. [PubMed] [Cross Ref]
77. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004;22(12):1567–72. doi: 10.1038/nbt1037. [PubMed] [Cross Ref]
78. MacDonald GH, Rubel EW. Three-dimensional imaging of the intact mouse cochlea by fluorescent laser scanning confocal microscopy. Hear Res. 2008;243(1–2):1–10. doi: 10.1016/j.heares.2008.05.009. [PMC free article] [PubMed] [Cross Ref]
79. Cui C, Lansford R, Filla MB, Little CD, Cheuvront TJ, Rongish BJ. Electroporation and EGFP labeling of gastrulating quail embryos. Dev Dyn. 2006;235(10):2802–10. doi: 10.1002/dvdy.20895. Epub 2006/08/09. [PubMed] [Cross Ref]
80. Rupp PA, Filla MB, Cui C, Little CD. Chapter 5. Avian embryos a model for the study of primary vascular assembly in warm-blooded animals. Methods Enzymol. 2008;445:107–23. doi: 10.1016/S0076-6879(08)03005-X. [PubMed] [Cross Ref]
81. Czirok A, Rupp PA, Rongish BJ, Little CD. Multi-field 3D scanning light microscopy of early embryogenesis. J Microsc. 2002;206(Pt 3):209–17. [PubMed]
82. Rupp PA, Czirok A, Little CD. alphavbeta3 integrin-dependent endothelial cell dynamics in vivo. Development. 2004;131(12):2887–97. doi: 10.1242/dev.01160. [PubMed] [Cross Ref]
83. Rupp PA, Rongish BJ, Czirok A, Little CD. Culturing of avian embryos for time-lapse imaging. Biotechniques. 2003;34(2):274–8. [PubMed]
84. Erickson CA, Duong TD, Tosney KW. Descriptive and experimental analysis of the dispersion of neural crest cells along the dorsolateral path and their entry into ectoderm in the chick embryo. Dev Biol. 1992;151(1):251–72. [PubMed]
85. Rawles ME. Origin of melanophores and their role in development of color patterns in vertebrates. Physiol Rev. 1948;28(4):383–408. [PubMed]
86. Erickson CA, Goins TL. Avian neural crest cells can migrate in the dorsolateral path only if they are specified as melanocytes. Development. 1995;121(3):915–24. [PubMed]
87. Ebner Vv. Urwirbel und Neugliederung der Wirbelsäule. 1888
88. Coffin JD, Poole TJ. Embryonic vascular development: immunohistochemical identification of the origin and subsequent morphogenesis of the major vessel primordia in quail embryos. Development. 1988;102(4):735–48. [PubMed]
89. Isogai S, Horiguchi M, Weinstein BM. The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. Dev Biol. 2001;230(2):278–301. doi: 10.1006/dbio.2000.9995. [PubMed] [Cross Ref]
90. Poynter G, Lansford R. Generating transgenic quail using lentiviruses. Methods Cell Biol. 2008;87:281–93. Epub 2008/05/20. doi: S0091-679X(08)00215-X [pii] 10.1016/S0091-679X(08)00215-X. [PubMed]
91. Wilting J, Brand-Saberi B, Huang R, Zhi Q, Kontges G, Ordahl CP, Christ B. Angiogenic potential of the avian somite. Dev Dyn. 1995;202(2):165–71. doi: 10.1002/aja.1002020208. [PubMed] [Cross Ref]
92. Keynes RJ, Stern CD. Segmentation in the vertebrate nervous system. Nature. 1984;310(5980):786–9. [PubMed]
93. Seidl AH, Sanchez JT, Schecterson L, Tabor KM, Wang Y, Kashima DT, Poynter G, Huss D, Fraser SE, Lansford R, Rubel EW. Transgenic quail as a model for research in the avian nervous system: a comparative study of the auditory brainstem. J Comp Neurol. 2013;521(1):5–23. doi: 10.1002/cne.23187. [PMC free article] [PubMed] [Cross Ref]
94. Ma Q, Fode C, Guillemot F, Anderson DJ. Neurogenin1 and neurogenin2 control two distinct waves of neurogenesis in developing dorsal root ganglia. Genes Dev. 1999;13(13):1717–28. Epub 1999/07/10. [PubMed]
95. Marmigere F, Ernfors P. Specification and connectivity of neuronal subtypes in the sensory lineage. Nat Rev Neurosci. 2007;8(2):114–27. Epub 2007/01/24. doi: nrn2057 [pii] 10.1038/nrn2057. [PubMed]
96. Rifkin JT, Todd VJ, Anderson LW, Lefcort F. Dynamic expression of neurotrophin receptors during sensory neuron genesis and differentiation. Dev Biol. 2000;227(2):465–80. Epub 2000/11/10. doi: 10.1006/dbio.2000.9841 S0012–1606(00)99841-5 [pii] [PubMed]
97. Carr VM, Simpson SB., Jr Proliferative and degenerative events in the early development of chick dorsal root ganglia. II. Responses to altered peripheral fields. J Comp Neurol. 1978;182(4):741–55. doi: 10.1002/cne.901820411. Epub 1978/12/15. [PubMed] [Cross Ref]
98. Wakamatsu Y, Maynard TM, Weston JA. Fate determination of neural crest cells by NOTCH-mediated lateral inhibition and asymmetrical cell division during gangliogenesis. Development. 2000;127(13):2811–21. [PubMed]
99. Taipale J, Keski-Oja J. Growth factors in the extracellular matrix. FASEB J. 1997;11(1):51–9. [PubMed]
100. Lichtman JW, Fraser SE. The neuronal naturalist: watching neurons in their native habitat. Nat Neurosci. 2001;4(Suppl):1215–20. doi: 10.1038/nn754. [PubMed] [Cross Ref]
101. Gore AV, Monzo K, Cha YR, Pan W, Weinstein BM. Vascular development in the zebrafish. Cold Spring Harb Perspect Med. 2012;2(5):a006684. doi: 10.1101/cshperspect.a006684. [PMC free article] [PubMed] [Cross Ref]
102. Stainier DY, Weinstein BM, Detrich HW, 3rd, Zon LI, Fishman MC. Cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. Development. 1995;121(10):3141–50. [PubMed]
103. Thiery J-P, Duband JL. Role of tissue environment and fibronectin in the patterning of neural crest derivatives. Trends Neurosci. 1986;9:565–70.
104. Thiery JP, Duband JL, Tucker GC. Cell migration in the vertebrate embryo: role of cell adhesion and tissue environment in pattern formation. Annu Rev Cell Biol. 1985;1:91–113. doi: 10.1146/annurev.cb.01.110185.000515. [PubMed] [Cross Ref]
105. Wang HU, Anderson DJ. Eph family transmembrane ligands can mediate repulsive guidance of trunk neural crest migration and motor axon outgrowth. Neuron. 1997;18(3):383–96. [PubMed]
106. Santiago A, Erickson CA. Ephrin-B ligands play a dual role in the control of neural crest cell migration. Development. 2002;129(15):3621–32. [PubMed]
107. Acevedo LM, Lindquist JN, Walsh BM, Sia P, Cimadamore F, Chen C, Denzel M, Pernia CD, Ranscht B, Terskikh A, Snyder EY, Cheresh DA. hESC Differentiation toward an Autonomic Neuronal Cell Fate Depends on Distinct Cues from the Co-Patterning Vasculature. Stem Cell Reports. 2015;4(6):1075–88. doi: 10.1016/j.stemcr.2015.04.013. [PMC free article] [PubMed] [Cross Ref]
108. Beall AC, Rosenquist TH. Smooth muscle cells of neural crest origin form the aorticopulmonary septum in the avian embryo. Anat Rec. 1990;226(3):360–6. doi: 10.1002/ar.1092260313. [PubMed] [Cross Ref]
109. Etchevers HC, Vincent C, Le Douarin NM, Couly GF. The cephalic neural crest provides pericytes and smooth muscle cells to all blood vessels of the face and forebrain. Development. 2001;128(7):1059–68. Epub 2001/03/14. [PubMed]
110. Jiang X, Rowitch DH, Soriano P, McMahon AP, Sucov HM. Fate of the mammalian cardiac neural crest. Development. 2000;127(8):1607–16. [PubMed]
111. Li L, Fukunaga-Kalabis M, Herlyn M. Isolation, characterization, and differentiation of human multipotent dermal stem cells. Methods Mol Biol. 2013;989:235–46. doi: 10.1007/978-1-62703-330-5_18. [PubMed] [Cross Ref]
112. Liang D, Wang X, Mittal A, Dhiman S, Hou SY, Degenhardt K, Astrof S. Mesodermal expression of integrin alpha5beta1 regulates neural crest development and cardiovascular morphogenesis. Dev Biol. 2014;395(2):232–44. doi: 10.1016/j.ydbio.2014.09.014. [PMC free article] [PubMed] [Cross Ref]
113. Reissmann E, Ernsberger U, Francis-West PH, Rueger D, Brickell PM, Rohrer H. Involvement of bone morphogenetic protein-4 and bone morphogenetic protein-7 in the differentiation of the adrenergic phenotype in developing sympathetic neurons. Development. 1996;122(7):2079–88. [PubMed]
114. Fortuna V, Pardanaud L, Brunet I, Ola R, Ristori E, Santoro MM, Nicoli S, Eichmann A. Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons. Cell reports. 2015;11(11):1786–96. doi: 10.1016/j.celrep.2015.05.028. [PubMed] [Cross Ref]
115. Plein A, Calmont A, Fantin A, Denti L, Anderson NA, Scambler PJ, Ruhrberg C. Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest. 2015;125(7):2661–76. doi: 10.1172/JCI79668. [PMC free article] [PubMed] [Cross Ref]
116. Wiszniak S, Mackenzie FE, Anderson P, Kabbara S, Ruhrberg C, Schwarz Q. Neural crest cell-derived VEGF promotes embryonic jaw extension. Proc Natl Acad Sci U S A. 2015;112(19):6086–91. doi: 10.1073/pnas.1419368112. [PubMed] [Cross Ref]
117. Milgrom-Hoffman M, Michailovici I, Ferrara N, Zelzer E, Tzahor E. Endothelial cells regulate neural crest and second heart field morphogenesis. Biol Open. 2014;3(8):679–88. doi: 10.1242/bio.20148078. [PMC free article] [PubMed] [Cross Ref]