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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Immunol. Author manuscript; available in PMC 2017 March 15.
Published in final edited form as:
J Immunol. 2016 March 15; 196(6): 2526–2540.
Published online 2016 February 15. doi: 10.4049/jimmunol.1502537

Figure 2

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Proliferation, death, and accumulation of stimulated Treg. efluor670-labeled wild type and T-bet−/− nTreg were stimulated with IL2, anti-CD28, and/or anti-CD3. Three days later, cells were stained with viability dye eflour450 and (A) proliferation (efluor670 dilution), (B) cell death (Efluor450 staining) and (C) accumulation (total viable cell numbers) were enumerated along with Fox3 protein expression used to define and enumerate cells. *P < 0.05, **P < 0.005, ***P < 0.0005.

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