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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Physiol Behav. Author manuscript; available in PMC 2016 July 1.
Published in final edited form as:
PMCID: PMC4777966
NIHMSID: NIHMS683328

The Brain on Stress: Insight from Studies Using the Visible Burrow System

Abstract

The discovery of adrenal steroid receptors outside of the hypothalamus in the hippocampus and other forebrain regions catalyzed research on the effects of stress upon cognitive function, emotions and self-regulatory behaviors as well as the molecular, cellular and neuroanatomical mechanisms underlying acute and chronic stress effects on the brain. Indeed, this work has shown that the brain is a plastic and vulnerable organ in the face of acute and chronic stress. The insight that Bob and Caroline Blanchard had in developing and interpreting findings using the Visible Burrow System model made an enormous contribution to the current view that the human brain is very sensitive to the social environment and to agonistic interactions between individuals. Their collaboration with Sakai and McEwen at The Rockefeller University extended application of the Visible Burrow System model to demonstrate that it also was a unique and highly relevant neuroethological model with which to study stress and adaptation to stressors. Those studies focused on the brain and systemic organ responses to stress and, in turn, described that the brain is also very responsive to changes in systemic physiology.

1. Introduction

Both the word and concept of “stress” were introduced by Hans Selye with an emphasis on physical stressors such as physical injury, heat and cold (1, 2) and later modified by the work of John Mason to include psychological stressors (3). One common response to this wide range of challenges was activation of the hypothalamic-pituitary-adrenal (HPA) axis, ultimately leading to an increase in glucocorticoid secretion by the adrenal cortex. Neuroendocrinology developed as a discipline that explored the hypothalamic control of pituitary function (4) and largely focused upon the feedback regulation of hormone secretion at the level of these two structures, without reference to the rest of the brain. Now there is a broader view of neuroendocrinology that emphasizes the bidirectional communication between brain and body and the recognition that the social as well as physical environment plays a powerful role in shaping individuals over the lifecourse via this bidirectional communication (5).

What led to this outlook? We contributed one key finding when we discovered glucocorticoid receptors outside of the hypothalamus using cellular uptake and nuclear retention of radiolabeled corticosterone; this binding was initially found in the hippocampal formation of the rat (68) and later observed in the primate as well (9). Subsequent discoveries by many investigators demonstrated the importance of adrenal steroids and other hormones in mediating both adaptation and damage in the hippocampus and other forebrain areas, but it was not until we collaborated with Bob and Caroline Blanchard that we came to appreciate the role of the social environment in the regulation of these endocrine and neuromodulatory effects.

2. Functional significance of adrenal steroid receptors in the brain

What is the function of such receptors in hippocampus? The initial focus for the hippocampus was on inhibitory behaviors and sleep (1013). Then, Robert Sapolsky, as a student and then postdoctoral fellow in our laboratory, developed the “glucocorticoid cascade hypothesis” of stress and aging with McEwen and Lewis Krey; this concept placed an emphasis on wear and tear upon the brain and body, mediated by cortisol feedback on the hippocampus, over a stressful life and as part of the aging process (14). By that time, neural actions of adrenal steroids were shown by Reul and de Kloet to involve both Type I (mineralocorticoid) and Type II (glucocorticoid) receptors (15). Meanwhile, biphasic actions of adrenal steroids were recognized on memory (16, 17), long-term potentiation (18), appetite and locomotor activity during the diurnal cycle (see (19)) and later, on acquired immunity (20). Interestingly, the memory and immune-enhancing effects of acute stress were also shown to involve catecholamines, which are released by the sympathetic nervous system under stressful conditions (21, 22).

But there were other, fundamental actions of adrenal steroids yet to be discovered when Elizabeth Gould joined the McEwen lab and introduced the Golgi method to visualize neuronal structure. This led to discovery of glucocorticoid and stress-induced remodeling of hippocampal neurons, particularly in the CA3 region (23, 24), an effect that was later shown by Magariños to involve excitatory amino acid mediation (2527). Gould, with Heather Cameron, also “rediscovered” adult neurogenesis in the dentate gyrus (28, 29), which had been shown previously by Altman (30) and Kaplan (31) but generally disregarded (32). Like hippocampal remodeling, dentate gyrus neurogenesis is also regulated by both glucocorticoids and excitatory amino acids (33).

However, regarding both dendritic remodeling and postnatal neurogenesis, there remained the task of showing generality across mammalian species. Both Gould and Magariños worked with Eberhard Fuchs on his tree shrew variant of a resident-intruder paradigm that examines the consequences of stressful social interactions. Gould and colleagues demonstrated postnatal neurogenesis both in tree shrew model and in macaque hippocampus (34, 35). The phenomenon of CA3 dendritic remodeling was also shown to occur in the tree shrew by Magariños and Fuchs (36) and in hibernating hamsters by Magariños and Paul Pevet (37).

But that gets ahead of the story, because, until we began to collaborate with Bob and Caroline Blanchard at the University of Hawaii, we had not fully appreciated the role of social behavior and social hierarchies as stressors, even though Mason had shown that the psychological stress of one monkey seeing another getting food was an enormously effective stressor as indicated by an elevation in cortisol (38).

3. Studies using the Visible Burrow System with the Blanchards

Around the same time that our lab was exploring the effects of steroid hormones on brain function, Bob and Caroline Blanchard were developing experimental methods to study rodent behavior in naturalistic settings. Rats are normally social animals, living in mixed-sex groups of up to several hundred animals in complex underground habitats consisting of interconnecting tunnel and burrow systems (3941). Within these larger societies, rats form smaller single-sex and mixed-sex colonies of three to 20 animals. These underground habitats made the rat difficult to study in the wild, so the Blanchards attempted to recreate these conditions in the laboratory. Their initial studies simply housed mixed-sex groups of Long-Evans rats in a large open area (“colony” condition), where dominance hierarchies quickly formed among the males, but not the females (42). They then attempted to create a more naturalistic laboratory model by providing the animals with a dirt substrate in which they could create their own burrows and tunnels. The males housed in the burrowing habitats showed significantly greater aggression toward intruders than the “colony” animals; furthermore, a significant percentage of subordinate animals died within four months of living in the habitats, whereas there was no mortality among the “colony” subordinates (43). The elevated mortality among subordinate animals was consistent with that observed by Barnett (40) and Calhoun (41), and appeared to be related more to the physiological effects of social subordination, as opposed to lethal wounding, as the frequency of fighting diminished with duration of housing.

While the burrowing habitats effectively recreated key environmental and behavioral features of rat colonies in the wild, the Blanchards soon realized that once the animals started burrowing, they were effectively “underground” and, therefore, just as difficult to observe as in the wild. Their next step—the “visible burrow system” or “VBS” —was a stroke of creative genius. Rather than having the rats dig their own tunnels and burrows, Bob and Caroline Blanchard built the burrows for them, equipping them with dark sides and limited light to simulate underground conditions for the rats, with clear plexiglas tunnels and chambers with clear tops to facilitate observation by the experimenters (44). The design of the VBS apparatus has evolved into the one diagrammed in Figure 1.

Figure 1
Diagram of a Visible Burrow System (VBS)

As in the wild, one of the key features of the VBS colony model is the use of mixed sex groups, generally consisting of 4–5 male and 2–3 female Long-Evans rats. Initial studies used singly housed males as controls, while subsequent iterations used male-female pairs, to allow for sexual interactions and reduce any adverse effects of social isolation. The rats are introduced into an open area, where they have access to food and water, as well as tunnels leading to several other compartments (44). The males quickly form dominance hierarchies, with the dominant guarding access to food and water reservoirs and attempting, somewhat unsuccessfully, to limit access to the females as well. The hierarchies are usually established during the first few days of group housing, with the dominants and subordinates being readily identified by distinctive patterns of offensive or defensive wounding; the subordinates generally lose a significant amount of body weight during this period as well (45). The colonies are generally studied for periods of approximately two weeks, after which mortality among the subordinates becomes a problem.

Randall Sakai, at the time a post-doctoral fellow with McEwen, was key in initiating the VBS collaborations between the McEwen and Blanchard Laboratories in 1989. Our studies with the Blanchards using the VBS model have demonstrated profound consequences of chronic subordination stress. These changes suggest that subordination, a common and consistent feature of life for many animals living in social groups, may be a particularly relevant model for investigating the behavioral, neural and endocrine correlates of chronic stress (46). Over the years, we have systematically shown alterations in hormone activity and regulation, metabolic activity, reproductive function, neurochemistry and neuronal morphology in subordinate animals; furthermore, we have shown that there are costs associated with being the dominant as well (summarized in Table 1).

Table 1
Behavioral, physiological, and endocrine consequences of chronic social stress in rodents.

3.1. Effects on the HPA axis

Perhaps the most consistent findings in the VBS model have been the effects of social stress on various indices of HPA axis activity. After removal from the VBS, plasma corticosterone (CORT) levels were consistently higher in the subordinate animals, and often elevated in the dominant animals as well, when compared to controls (Blanchard et al. 1993, 1995; McKittrick et al. 1995). In the dominant animals, the increased CORT appears to be context-dependent, however, as the elevated CORT was seen only immediately upon removal from the VBS, but not after the animals have been returned to their home cage for an hour (47). In contrast, in the subordinates, the baseline elevations in CORT persisted for at least an hour after removal from the VBS.

In addition to changes in baseline and VBS context-dependent CORT levels, subordinates often exhibited a blunted response to a novel stressor, suggesting more complex dysfunction of the HPA axis. Close examination the stress response in individual animals, however, led us to conclude that the subordinate rats did not constitute a uniform group, but rather could be subdivided into “stress-responsive” (SRS) or “non-responsive” (NRS) subordinates, depending on their glucocorticoid response to the novel applied stressor (46)(Figure 2). This classification of subordinates proved useful in subsequent studies as the NRS group generally exhibited the most adverse consequences of subordination (cf. effects on testosterone, CBG, etc.). We have observed that NRS do not mount a normal stress response prior to 7 days after removal from the VBS, thus the NRS phenotype persists even during a recovery period and is unlikely that it is context dependent. The proportion of subordinate rats that exhibit a NRS stress response profile was smaller once commercially-bred Long Evans rats were used rather than the colony-bred rats used in the University of Hawaii studies perhaps suggesting a role for genetic factors or intensity of stress experienced while in the VBS. The relative contribution of these factors to the NRS phenotype has not yet been systematically examined in this model.

Figure 2
Corticosterone response to acute novel stressor following 14-day chronic social stress in the VBS

VBS housing led to changes in other determinants of HPA function within the hippocampus and other brain areas. In the hippocampus, subordinates showed lower levels of mRNA for both Type I (MR) and Type II (GR) glucocorticoid receptors compared to controls (48); this was consistent with other studies indicating compensatory downregulation of these receptors in response to elevated levels of glucocorticoids. The corticotropin releasing hormone (CRH) system was also affected in both hypothalamic and extrahypothalamic brain regions. Non-responsive subordinates (NRS) expressed a significantly lower average number of CRH mRNA grains per cell in the paraventricular hypothalamic nucleus (PVN) compared with SRS, dominants, or cage-housed control rats (49) (Figure 3A). The number of cells containing CRH mRNA was also significantly lower in NRS than in SRS or dominants (Figure 3B). The changes in the PVN were limited to CRH, since arginine vasopressin (AVP) mRNA levels did not vary with behavioral rank in this area. The blunted CORT response to the novel stressor may be a direct consequence of diminished synthesis and release of CRH seen in the NRS animals. The relationship between lower CRH levels and an impaired ability to mount a CORT response to a novel stressor is supported by the observation that no changes in CRH mRNA in the PVN were seen in a VBS experiment in which all of the subordinates had a normal stress response (Choi et al. 2006).

Figure 3
Dominance hierarchies and the CRH (CRF) and HPA system

In contrast to the PVN, in the central amygdala, CRH mRNA levels were increased in both groups of subordinates compared with control rats, whereas SRS exhibited higher levels than the dominants rats as well (49) (Figure 3C). However, in the medial amygdala, the number of cells expressing AVP mRNA was significantly greater in control rats compared with both groups of subordinates; in addition, the number of AVP-positive cells significantly correlated with plasma testosterone level. In another study, CRH levels were also increased in the bed nucleus of the stria terminalis in the subordinates (50). It has been postulated that extrahypothalamic CRH may play a role in mediating stress-induced behavioral changes (51).

The VBS studies with the Blanchards revealed manifestations of social stressors on systemic regulation of HPA activity as well. Compared to controls, VBS-housed animals all showed a decrease in corticosteroid binding globulin (CBG), which plays a role in regulating the levels of free bioavailable CORT (52). There was a large reduction in plasma CBG levels of subordinate (−70%) and dominant (−40%) rats relative to control rats, and there was a significant correlation between plasma CBG level and available Type II (or GR) receptors in the spleen. Corticosteroid receptor levels in these adrenal-intact animals reflect the number of receptors that were unoccupied by endogenous hormone at the time of sacrifice. As a result of the reduced CBG, both subordinate and dominant rats had fewer available splenic Type II receptors than control rats, suggesting that a greater proportion of receptors were occupied and activated by endogenous hormone at the time of sacrifice in these animals than in control rats. These results suggest that a decrease in CBG levels as a result of chronic social stress led to greater access of free CORT hormone to Type II receptors in the spleen than is typically present in rats under basal or acute stress conditions. This increased stimulation of splenic Type II glucocorticoid receptors could, in turn, provide a mechanism by which chronic stress may have a greater impact than acute stress on splenic immune function (52). The glucocorticoid resistance associated with social stress may have a compensatory role by moderating glucocorticoid suppression of immune responses to pathogens and wounds resulting from aggressive social interactions (53).

3.2. Effects on hypothalamic-pituitary-gonadal (HPG) axis

It is well established that the hypothalamic-pituitary-gonadal axis can be suppressed in response to chronic stress. Decreased testosterone is one of the first signs of stress-induced decline in reproductive function in males (54). Subordinate animals consistently have suppressed testosterone production during chronic social stress (5557). In studies using the VBS model, plasma luteinizing hormone (LH) and plasma testosterone levels of subordinates are poorly correlated across time, relative to what occurs in both controls and dominants (54). Plasma LH levels of subordinates remain similar to those of control through at least 1 week of social stress, and are lower than both the control and dominant groups by the end of 2 weeks of VBS housing. In contrast, plasma testosterone levels in subordinates are already lower than those of dominant rats by Day 2 of VBS housing, a time point at which subordinate plasma LH levels are still normal. Thus, the reduction in plasma testosterone in subordinates occurs prior to a decrease in LH suggesting that chronic stress may have influences on the HPG axis at multiple levels to affect testosterone production and reproductive function.

In collaboration with Matt Hardy at the Population Council, we found that the decrease in testosterone levels in subordinate rats may be explained, at least in part, by the failure of Leydig cells to increase 11βHSD oxidative activity to compensate for higher circulating glucocorticoids (54). High circulating levels of glucocorticoids inhibit the Leydig cell’s production and secretion of testosterone. Thus, the Leydig cells are normally protected from high circulating plasma glucocorticoids by 11βHSD, which catalyzes the conversion of biologically active glucocorticoids into their inert 11-keto metabolites, thereby allowing for normal steroidogenesis to occur. Subordinate rats have reduced 11βHSD protein levels and reduced 11βHSD activity, in contrast to dominant and control animals who have normal plasma testosterone levels and similar levels of 11βHSD protein and enzyme activity (58). Of note, however, is that reductions in plasma testosterone may occur as early as Day 2 of VBS housing, and have been observed prior to elevated basal levels of corticosterone, again indicating that testosterone production is modulated by multiple factors.

3.3. Effects on serotonin systems

Animals housed in the VBS showed alterations in several neurotransmitter systems. One of the Blanchards’ earliest studies of the VBS showed that defensive behavior in subordinate rats was associated with elevated 5HIAA/5HT ratios in several brain regions (59). Furthermore, the subordinates exhibited changes in 5HT1A and 5HT2A receptor binding that mimicked those seen in our laboratory and others in response to chronic stress (e.g. (6062) (Figure 4).

Figure 4
Summary of the neurobiological changes in the brains of subordinate rats following chronic social stress.

Regarding HPA activity and serotonin, subordinate rats had elevated basal corticosterone (CORT) levels relative to dominants and individually housed controls. Stress responsive subordinates (SRS) showed reduced binding to 5-HT1A receptors compared to controls throughout hippocampus and dentate gyrus (Figure 4). NRS showed decreased binding only in CA3 of hippocampus. In addition, dominants showed decreased 5-HT1A binding in CA1, CA3, and CA4 compared to controls. Both responsive and nonresponsive subordinates showed increased binding to 5-HT2 receptors in parietal cortex compared to controls. No changes were observed in binding to 5-HT1B receptors (63). Moreover, both dominants and subordinates showed decreased binding to the serotonin transporter (5HTT) in Ammon’s horn compared to controls, while 5HTT binding remained unchanged in dentate gyrus and raphe (47). The functional significance of these changes is unclear; however, alterations in serotonergic function are thought to be involved in several stress-associated disorders, such as major depression, PTSD and anxiety disorders.

3.4. Effects on catecholamine systems

The neurochemical consequences of chronic social stress were diverse and widespread, affecting not only the serotonergic system, but also noradrenergic and dopaminergic pathways as well. Tyrosine hydroxylase (TH) mRNA levels measured with in situ hybridization were elevated in locus coeruleus (LC) of non-responsive subordinates (NRS) compared to singly or pair-housed controls; NRS also had higher TH levels than dominants (64). TH protein levels determined by immunoautoradiography were also higher in LC of NRS and stress responsive subordinates (SRS) versus pair-housed controls (Figure 4). Since the activity of LC neurons is increased in response to stress (Jacobs et al., 1991), it is likely that the changes in TH allow an increase in the synthetic capacity of the noradrenergic neurons in order to compensate for stress-related demands upon the system.

The dopaminergic system also responded to the social stress of the VBS (65). For this study rats were exposed for 2 weeks to the VBS and classified as SRS and NRS. After a brief recovery period, half of the cohorts were subsequently returned to the VBS for two additional cycles of reexposure and recovery. NRS rats showed decreased dopamine transporter density after a single VBS exposure in the dorsolateral caudate putamen (DLCPu) and also showed decreased DA transporter density in the nucleus accumbens (Acb) after repeated VBS exposure. In addition, dopamine D2 receptor density was elevated after a single VBS exposure in the Acb of both subordinate groups (SRS and NRS) and after repeated VBS exposure in the DLCPu, dorsomedial CPu, and Acb of NRS compared with controls. No changes in dopamine D1 receptor binding were observed in any group. Stress has long been known to increase vulnerability to drug abuse and addiction (66) and these finding provide a possible mechanism by which long-lasting changes in mesolimbic dopamine activity induced by chronic stress contribute to this vulnerability.

3.5. Neurogenesis and neuronal morphology in the hippocampal formation

The proliferation of new cells and the structure of existing neurons in the hippocampal formation were also affected by social hierarchies in the VBS. Although neurogenesis in the dentate gyrus did not differ between dominants and subordinates, more new neurons were observed in the dentate gyrus of the dominant males compared with both subordinates and controls (67). Under the conditions of this particular VBS system, which used Sprague-Dawley rats, dominant and subordinate animals did not differ in basal, stress or recovery levels of CORT and the also did not differ in thymus, adrenal gland, and body weights, suggesting that variables other than stress were responsible for the changes in adult neurogenesis. The differences in brain structure persisted among the animals that had no access to the burrow system after the dominance hierarchy stabilized, suggesting that social status rather than living in a complex environment accounted for the effect of dominance on adult neurogenesis.

Chronic social stress also led to significant remodeling of the apical dendritic trees of the pyramidal neurons in the hippocampus. There was a decrease in the number of branch points and total dendritic length in the apical dendritic trees of CA3 pyramidal neurons in dominant animals compared to unstressed controls; subordinates also had a decreased number of dendritic branch points (47)(Figure 5). The similarity of the changes in serotonin transporters (5HTT) binding, summarized above, and dendritic arborization between both groups of VBS animals, despite apparent differences in stressor severity experienced by the animals, suggests that these changes may be part of the normal adaptive response to chronic social stress (47). Yet, because of the greater dendritic shrinkage in dominants compared to subordinates, in spite of the larger adrenals in subordinates, these findings took us away from simplistic idea that CORT caused it all, and it also turned attention to the impact of the social environment, as will be discussed in the next section on the human brain.

Figure 5
Dendritic remodeling in CA3 hippocampal pyramidal neurons of dominant and subordinate rats in VBS

Indeed, the mechanism for dendritic shrinkage has subsequently been shown to involve multiple interacting mediators, with excitatory amino acid receptors playing a major role, along with other mediators such as brain derived neurotrophic factor (BDNF), tissue plasminogen activator (tPA), corticotrophin releasing hormone (CRH) and others; a more complete discussion of these mechanisms may be found elsewhere (27, 68).

4. Systemic effects of social stressors

Although the majority of the effects of social stress discussed above are focused on the brain, even the earliest studies of this model showed widespread effects on systemic physiology. The most basic observation of increased mortality and morbidity among the subordinates (42) demonstrated the consequences of chronic social stress on the overall health and wellbeing of the organism. The alterations in splenic glucocorticoid receptors discussed above may contribute to impaired immune responses in these animals; indeed, the subordinate animals in the VBS appeared to be particularly vulnerable to mycoplasmic respiratory infections endemic to the colony in Hawaii (Blanchard and Blanchard, unpublished observations).

Sakai went on to establish a 12 VBS facility in his laboratory at the University of Cincinnati in 2000 and has replicated many of the salient effects of social stress documented in the VBS experiments at the University of Hawaii. In addition, the Cincinnati studies used rats obtained from a commercial vendor indicating that the VBS model may be generalized to rat populations other than the in-house colony maintained in Hawaii. The consistency of the data and generalizability of the model emphasize the value and broad utility of the VBS model developed by Bob and Caroline Blanchard in studying social stress. Sakai and his colleagues extended use of the VBS model into investigations of the metabolic consequences of chronic stress, the role of androgens in social hierarchy formation and maintenance, and the influence of early life stress on social hierarchy development, among others.

Changes in body weight and metabolism were apparent early on (45). Although the decreases in body weight among colony animals were most dramatic in the versions of the VBS in which food was only available in the “surface” area, subordinate animals showed a rapid and sustained loss of up to 15% of their body weight, even when they were provided with food either during frequent “vacations” from the VBS, or within the burrows themselves (57, 69). Body weight of dominants was affected by VBS housing as well: the dominants typically lose a small but significant amount of weight in the VBS, and also fail to gain weight at the rate of the control animals (57). The metabolic basis of the weight loss differs between the two groups, as the subordinates lost both adipose and lean tissue, whereas the weight loss in the dominant animals was attributable to loss of adipose tissue along (56). Leptin and insulin levels have been shown to co-vary with adiposity (70) and these two hormones were decreased in the subordinate animals and, to a lesser extent, in the dominants as compared to controls.

An important adaptation to the VBS set up in Sakai’s lab was outfitting each apparatus with sophisticated computerized monitoring systems to measure food intake and determine meal patterns for each animal in the colony (identified by a subcutaneously implanted microchip) during chronic stress (Figure 1). Food intake is decreased in all animals upon introduction to the VBS; however, dominants quickly returned to their basal level of intake, while the subordinates showed a continued suppression in both the number and size of meals despite having access to food and water in all areas of the VBS; the diurnal pattern of meal consumption was also shifted among the subordinates perhaps suggesting altered circadian rhythms as well (71, 72).

When animals were removed from the VBS, subordinates were hyperphagic and quickly regained their body weight, but did so primarily as visceral adipose tissue while dominants regained body weight as both lean and adipose tissue (57, 72). Thus, body composition changed following social stress and this occurrence was hypothesized to be due to the different endocrine conditions that subordinate and dominant animals were in at the end of social housing in the VBS. That is, subordinates had low testosterone and high corticosterone levels and this state would promote adipose tissue deposition. In contrast, since dominant animals have testosterone levels similar to that of controls and corticosterone levels intermediate to that of controls and subordinates, dominants could regain weight as both lean and adipose tissue. Indeed, by treating subordinates with 5α-dihydrotestosterone (DHT), a non-aromatizable, reduced form of testosterone, weight loss during chronic stress in the VBS was not affected, but DHT promoted weight gain as lean body mass during recovery (55).

While the subordinates had lower levels of insulin and leptin than dominants and controls while in the VBS, they became hyperinsulinemic and hyperleptinemic following the recovery phase (57). The metabolic changes occurring in these animals suggest that chronic subordination may lead to a state that mimics metabolic syndrome in humans, as well as altering patterns of food intake in a way that may contribute to obesity (57, 73). These findings lead to consideration of potential interventions to promote weight gain in subordinates following chronic stress as lean muscle mass rather than adipose tissue. Amylin is a peptide hormone that is co-secreted with insulin from pancreatic β-cells and can act to reduce food intake and decrease weight gain and fat mass while preserving lean mass in obese subjects (74). Administering a low dose of amylin to rats after chronic stress in the VBS prevented hyperphagia in both dominant and subordinate rats for 3 days while having no effect on food intake in controls. Amylin suppressed fat mass gain in dominant and subordinate rats while lean mass was not affected. Thus, amylin treatment was effective in preventing greater post-stress adiposity in subordinate rats (75). Interventions such as these may be informative in developing useful clinical therapeutics as adverse metabolic conditions, such as obesity, diabetes and cardiovascular disease, are highly co-morbid with stress disorders and related mental illness.

These findings exemplify the important fact that stressors and stress hormones have complex effects on systemic physiology, including adaptive responses that may be facilitated by via acute secretion of glucocorticoids and catecholamines, followed by potentially detrimental changes elicited by chronic stress and persistent high glucocorticoid levels. More broadly, along with the glucocorticoid cascade hypothesis by Robert Sapolsky (14), these findings contributed to the concepts of allostasis and allostatic load, emphasizing the protective as well as damaging effects of stress on the brain and body, operating through the brain (5, 76).

5. Translation to and from the human brain

Using the perspective of allostatic load (77), extension of these findings to the human brain has revealed the enormous range of influences of social factors and health behaviors in hippocampal health and manifestations throughout the brain and body. Studies of the human hippocampus have demonstrated shrinkage of the hippocampus not only in mild cognitive impairment and Alzheimer’s disease (78), but also in Type 2 diabetes (79), prolonged major depression (80), Cushing’s disease (81) and post-traumatic stress disorder (PTSD) (82). Moreover, in non-disease conditions, such as a 20 year history of chronic stress (83), chronic inflammation (84), lack of physical activity (85) and jet lag (86), smaller hippocampal or temporal lobe volumes have been reported. Regarding the underlying cause of volume reduction, autopsy studies on depression-suicide have indicated loss of glial cells and smaller neuron soma size (87), which is indicative of a smaller dendritic tree. This is consistent with the findings summarized above from animal models.

With regard to Type 2 diabetes, it should be emphasized that the hippocampus has receptors for, and the ability to take up and respond to insulin, ghrelin, insulin-like growth factor-1 (IGF-1) and leptin; IGF-1 has also been shown to mediate exercise-induced neurogenesis (88). Thus, besides its response to glucocorticoids, the hippocampus is an important target of metabolic hormones that have a variety of adaptive actions in the healthy brain; these functions are likely to be perturbed in metabolic disorders, such as diabetes (88).

The metabolic syndrome, which includes insulin resistance associated with cardiovascular risk factors and obesity, shows a strong relationship to socioeconomic status (SES), (89, 90). Obesity and Type 2 diabetes in low SES teenagers are associated with impaired cognitive impairment, smaller hippocampal volume and altered white matter structure (91). The effects of lower SES extend to other brain regions such as the prefrontal cortex where lower SES is associated with reduced gray matter volume (92), and with obesity, systemic inflammation and altered white matter structure (93, 94). Thus the social environment over the lifecourse plays a powerful role in shaping the brain (77, 95).

But the hippocampus is not the only brain region affected. The young adult human prefrontal cortex reflects the effects of chronic stress by showing impaired cognitive flexibility and reduced functional connectivity that parallels the effects of stress in the young adult rat brain, including the reversibility after the end of the stressful period (9699). The studies of circadian disruption complement those on the hippocampus/temporal lobe noted above in flight crews suffering from chronic jet lag (86) and raise important questions about how the brain handles shift work, jet lag and chronic sleep deprivation (100). Furthermore, aging in rats is associated with failure to spontaneously reverse shrinking of medial prefrontal cortical neurons after chronic stress (99) and this harkens back to the glucocorticoid cascade hypothesis (14).

Indeed, when brain circuits remain changed, there are behavioral states and cognitive impairments that also remain and some of these may be maladaptive. Amygdala overactivity is a consequence of exposure to traumatic stressors in a PTSD-like animal model that produces a delayed increase in spine density in basolateral amygdala along with a delayed increase in anxiety-like behavior (101). Amygdala overactivity is also associated with mood disorders (102) and amygdala enlargement is reported in children of chronically depressed mothers (103). Similarly, hippocampal volume reduction in prolonged depression, Type 2 diabetes and Cushing’s disease is associated with cognitive and mood impairment (79, 80, 104, 105). These conditions require external intervention that may include use of antidepressants (106), surgery to reduce hypercortisolemia (81), regular physical activity (107) and mindfulness-based stress reduction (108).

6. Closing perspectives

The insight Bob and Caroline Blanchard had in developing and interpreting the findings from the Visible Burrow System made an enormous contribution to the current view that the human brain is very sensitive to the social environment and to agonistic interactions between individuals. Their collaboration with Sakai and McEwen at the Rockefeller extended application of the Visible Burrow System model to demonstrate that it also was a unique and highly relevant neuroethological model with which to study stress and adaptation to stressors. These studies focused on the brain and systemic organ responses to stress and, in turn, described that the brain is also very responsive to changes in systemic physiology.

Finally, we would like to close on a more personal note. Bob Blanchard’s scientific contributions to and influence on the field of behavioral neuroscience is clear and examples of this are elegantly discussed in this Special Issue. What may be less often talked about is Bob’s commitment to training and providing high caliber biomedical research opportunities to undergraduate students at the University of Hawaii, particularly to underrepresented minority students, the vast majority of whom have gone on to professional careers in biomedical research and medicine. But Bob’s influence did not stop there. With the establishment of the collaboration at The Rockefeller University with the McEwen lab and the expansion of use of the Visible Burrow System model at the University of Cincinnati in the Sakai lab, Bob’s reach extended from the middle of the Pacific to have national and international impact. Two of the co-authors of this manuscript, McKittrick and Tamashiro, used the Visible Burrow System social stress model in their Ph.D. research projects. Other graduate students and post-doctoral fellows at Rockefeller and Cincinnati also capitalized on the model, continued to build upon the foundation the Blanchards established in Hawaii, and have gone on to successful professional careers of their own, including Drs. David S. Albeck (University of Colorado-Denver), Dennis C. Choi (Emory University), Jon F. Davis (Washington State University), Elizabeth Duncan-Vaidya (Cuyahoga Community College), Eric G. Krause (University of Florida), Susan J. Melhorn (University of Washington), Mary M.N. Nguyen-Choi (Booz-Allen Hamilton), Karen A. Scott (University College Cork, Ireland), Robert L. Spencer (University of Colorado-Boulder), Yoshifumi Watanabe (Yamaguchi University, Japan), and others too numerous to list here. It is clear that Bob Blanchard and his wife Caroline have had, and will continue to have, a significant influence on future generations of scientists and educators far and wide as Bob’s legacy lives on. Mahalo nui loa, Bob.

Figure 6
Summary of systemic effects of social subordination on components of the HPA axis.
Table 2
Neurobiological changes following chronic social stress in rodents.

Highlights

  • The social environment is important for behavioral and neuroendocrine function.
  • The VBS is a relevant neuroethological animal model to study social stress.
  • Social subordination stress has widespread effects on brain, behavior and physiology.
  • Dominant animals also experience consequences of social stress.
  • Bob Blanchard’s legacy is perpetuated through his influence on trainees.

Acknowledgments

The authors thank Dr. Li Yun Ma for her many years of expert technical support and training of numerous students and fellows. We gratefully acknowledge the research funding that supported this work: NSF IBN-9528213 (RJB, DCB, RRS, BSMc), NIH R01DK066596 (RRS), R01MH-41256 (BSMc), F31NS047791 (KLKT), F31MH088230 (KAS), The H.F. Guggenheim Foundation (RRS), and Brain and Behavior Research Foundation (NARSAD) Independent Investigator Grant (RRS).

Footnotes

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References

1. Selye H. A syndrome produced by diverse nocuous agents. Nature. 1936;138:32. [PubMed]
2. Selye H, editor. The Stress of Life. New York: McGraw Hill; 1956.
3. Mason J. Psychological influences on the pituitary-adrenal cortical system. In: Pincus G, editor. Recent Progress in Hormone Research. New York: Academic Press; 1959. pp. 345–89.
4. Harris GW. Effects of the nervous system on the pituitary-adrenal activity. Prog Brain Res. 1970;32:86–8. [PubMed]
5. McEwen BS. Protective and Damaging Effects of Stress Mediators. New England J Med. 1998;338:171–9. [PubMed]
6. McEwen BS, Weiss J, Schwartz L. Selective retention of corticosterone by limbic structures in rat brain. Nature. 1968;220:911–2. [PubMed]
7. Gerlach J, McEwen BS. Rat brain binds adrenal steroid hormone: radioautography of hippocampus with corticosterone. Science. 1972;175:1133–6. [PubMed]
8. McEwen BS, Plapinger L. Association of corticosterone-1,2 3H with macromolecules extracted from brain cell nuclei. Nature. 1970;226:263–4. [PubMed]
9. Gerlach J, McEwen BS, Pfaff DW, Moskovitz S, Ferin M, Carmel P, et al. Cells in regions of rhesus monkey brain and pituitary retain radioactive estradiol, corticosterone and cortisol differently. Brain Res. 1976;103:603–12. [PubMed]
10. Micco D, McEwen BS, Shein W. Adrenal steroid modulation of behavioral inhibition as revealed in appetitive extinction. J Comp Physiol Psychol. 1979;93:323–9. [PubMed]
11. Micco DJJ, McEwen BS, Shein W. Modulation of behavioral inhibition in appetitive extinction following manipulation of adrenal steroids in rats: Implications for involvement of the hippocampus. J Comp Physiol Psychol. 1979;93:323–9. [PubMed]
12. Micco D, Meyer J, McEwen BS. Effects of corticosterone replacement on the temporal patterning of activity and sleep in adrenalectomized rats. Brain Res. 1980;200:206–12. [PubMed]
13. Eichenbaum H, Otto T. The hippocampus - what does it do? Behav Neural Biol. 1992;57:2–36. [PubMed]
14. Sapolsky R, Krey L, McEwen BS. The neuroendocrinology of stress and aging: the glucocorticoid cascade hypothesis. Endocr Rev. 1986;7:284–301. [PubMed]
15. Reul JM, DeKloet ER. Two receptor systems for corticosterone in rat brain: microdistribution and differential occupation. Endocrinology. 1985;117:2505–11. [PubMed]
16. Roozendaal B, Bohus B, McGaugh JL. Dose-dependent suppression of adrenocortical activity with metyrapone: effects on emotion and memory. Psychoneuroendocrinology. 1996;21(8):681–93. [PubMed]
17. Roozendaal B, McGaugh JL. Glucocorticoid receptor agonist and antagonist administration into the basolateral but not central amygdala modulates memory storage. Neurobiology of Learning and Memory. 1997;67:176–9. [PubMed]
18. Pavlides C, Watanabe Y, Magarinos AM, McEwen BS. Opposing role of adrenal steroid Type I and Type II receptors in hippocampal long-term potentiation. Neuroscience. 1995;68:387–94. [PubMed]
19. McEwen BS, Sakai RR, Spencer RL. Adrenal steroid effects on the brain: versatile hormones with good and bad effects. In: Schulkin J, editor. Hormonally-Induced Changes in Mind and Brain. San Diego: Academic Press; 1993. pp. 157–89.
20. Dhabhar FS, McEwen BS. Acute stress enhances while chronic stress suppresses cell-mediated immunity in vivo: a potential role for leukocyte trafficking. Brain Behav Immun. 1997;11:286–306. [PubMed]
21. Quirarte GL, Roozendaal B, McGaugh JL. Glucocorticoid enhancement of memory storage involves noradrenergic activation in the basolateral amygdala. Proc Natlacad Sci USA. 1997;94:14048–53. [PubMed]
22. Dhabhar F, McEwen B. Enhancing versus suppressive effects of stress hormones on skin immune function. Proc Natl Acad Sci USA. 1999;96:1059–64. [PubMed]
23. Watanabe Y, Gould E, McEwen BS. Stress induces atrophy of apical dendrites of hippocampus CA3 pyramidal neurons. Brain Res. 1992;588:341–4. [PubMed]
24. Watanabe Y, Gould E, Cameron HA, Daniels DC, McEwen BS. Phenytoin prevents stress- and corticosterone-induced atrophy of CA3 pyramidal neurons. Hippocampus. 1992;2:431–6. [PubMed]
25. Magarinos AM, McEwen BS. Stress-induced atrophy of apical dendrites of hippocampal CA3c neurons: involvement of glucocorticoid secretion and excitatory amino acid receptors. Neuroscience. 1995;69(1):89–98. [PubMed]
26. Magarinos AM, McEwen BS. Stress-Induced Atrophy of Apical Dendrites of Hippocampal CA3c Neurons: Comparison of Stressors. Neuroscience. 1995;69(1):83–8. [PubMed]
27. McEwen BS. Stress and hippocampal plasticity. Annu Rev Neurosci. 1999;22:105–22. [PubMed]
28. Gould E, Woolley C, McEwen BS. Short-term glucocorticoid manipulations affect neuronal morphology and survival in the adult dentate gyrus. Neuroscience. 1990;37:367–75. [PubMed]
29. Cameron HA, Gould E. Adult neurogenesis is regulated by adrenal steroids in the dentate gyrus. Neuroscience. 1994;61:203–9. [PubMed]
30. Altman J, Das GD. Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats. J Comp Neurol. 1965;124:319–36. [PubMed]
31. Kaplan MS, Bell DH. Neuronal proliferation in the 9-month-old rodent-radioautographic study of granule cells in the hippocampus. Exp Brain Res. 1983;52(1):1–5. Epub 1983/01/01. eng. [PubMed]
32. Kaplan MS. Environment complexity stimulates visual cortex neurogenesis: death of a dogma and a research career. Trends Neurosci. 2001 Oct;24(10):617–20. Epub 2001/09/29. eng. [PubMed]
33. Cameron HA, McEwen BS, Gould E. Regulation of adult neurogenesis by excitatory input and NMDA receptor activation in the dentate gyrus. J Neurosci. 1995;15:4687–92. [PubMed]
34. Gould E, McEwen BS, Tanapat P, Galea LAM, Fuchs E. Neurogenesis in the dentate gyrus of the adult tree shrew is regulated by psychosocial stress and NMDA receptor activation. J Neurosci. 1997;17:2492–8. [PubMed]
35. Gould E, Reeves AJ, Graziano MSA, Gross CG. Neurogenesis in the neocortex of adult primates. Science. 1999;286:548–52. [PubMed]
36. Magarinos AM, McEwen BS, Flugge G, Fuchs E. Chronic psychosocial stress causes apical dendritic atrophy of hippocampal CA3 pyramidal neurons in subordinate tree shrews. J Neurosci. 1996;16:3534–40. [PubMed]
37. Magarinos AM, McEwen BS, Saboureau M, Pevet P. Rapid and reversible changes in intrahippocampal connectivity during the course of hibernation in European hamsters. Proc Natl Acad Sci USA. 2006;103:18775–80. [PubMed]
38. Mason JW. Hormones And Metabolism: Psychological Influences on the Pituitary-Adrenal Cortical System. In: Pincus G, editor. Recent Progress In Hormone Research; Proceedings of the Laurentian Hormone Conference; 1958; New York and London: Academic Press; 1959. pp. 345–89.
39. Barnett SA. Behaviour of wild rats in the laboratory. Medical & biological illustration. 1956 Apr;6(2):104–11. [PubMed]
40. SAB . The Rat: A Study in Behavior. University of Chicago Press; 1958.
41. Calhoun JB. The Ecology and Sociology of the Norway Rat. Bethesda, MD: U.S. Department of Health, Education and Welfare; 1962.
42. Blanchard RJ, Blanchard DC, Flannelly KJ. Social stress, mortality and aggression in colonies and burrowing habitats. Behavioural processes. 1985 Aug;11(2):209–13. [PubMed]
43. Blanchard DC. Behavioral correlates of chronic dominance-subordination relationships of male rats in a seminatural situation. Neuroscience & Biobehavioral Reviews. 1990;14:455–62. [PubMed]
44. Blanchard RJ, Blanchard DC. Antipredator defensive behaviors in a visible burrow system. J Comp Psychol. 1989 Mar;103(1):70–82. [PubMed]
45. Blanchard DC, Sakai RR, McEwen B, Weiss SM, Blanchard RJ. Subordination stress: behavioral, brain, and neuroendocrine correlates. Behav Brain Res. 1993 Dec 20;58(1–2):113–21. [PubMed]
46. Blanchard DC, Sakai RR, McEwen BS, Weiss SM, Blanchard RJ. Subordination stress: behavioral, brain and neuroendocrine correlates. Behav Brain Res. 1993;58:113–21. [PubMed]
47. McKittrick CR, Magarinos AM, Blanchard DC, Blanchard RJ, McEwen BS, Sakai RR. Chronic social stress reduces dendritic arbors in CA3 of hippocampus and decreases binding to serotonin transporter sites. Synapse. 2000;36:85–94. [PubMed]
48. Chao H, Blanchard C, Blanchard R, McEwen BS, Sakai R. The effect of social stress on hippocampal gene expression. Mol Cell Neurosci. 1993;4:543–8. [PubMed]
49. Albeck DS, McKittrick CR, Blanchard DC, Blanchard RJ, Nikulina J, McEwen BS, et al. Chronic social stress alters levels of corticotropin-releasing factor and arginine vasopressin mRNA in rat brain. J Neurosci. 1997;17:4895–903. [PubMed]
50. Choi DC, Nguyen MM, Tamashiro KL, Ma LY, Sakai RR, Herman JP. Chronic social stress in the visible burrow system modulates stress-related gene expression in the bed nucleus of the stria terminalis. Physiol Behav. 2006 Oct 30;89(3):301–10. [PubMed]
51. Smagin GN, Heinrichs SC, Dunn AJ. The role of CRH in behavioral responses to stress. Peptides. 2001 May;22(5):713–24. [PubMed]
52. Spencer RL, Miller AH, Moday H, McEwen BS, Blanchard RJ, Blanchard DC, et al. Chronic Social Stress Produces Reductions In Available Splenic Type II Corticosteroid Receptor Binding and Plasma Corticosteroid Binding Globulin Levels. Psychoneuroendocrinology. 1996;21(1):95–109. [PubMed]
53. Quan N, Avitsur R, Stark JL, He L, Lai W, Dhabhar F, et al. Molecular mechanisms of glucocorticoid resistance in splenocytes of socially stressed male mice. J Neuroimmunol. 2003 Apr;137(1–2):51–8. Epub 2003/04/02. eng. [PubMed]
54. Hardy MP, Sottas CM, Ge R, McKittrick CR, Tamashiro KL, McEwen BS, et al. Trends of reproductive hormones in male rats during psychosocial stress: role of glucocorticoid metabolism in behavioral dominance. Biol Reprod. 2002 Dec;67(6):1750–5. [PubMed]
55. Nguyen MM, Tamashiro KL, Melhorn SJ, Ma LY, Gardner SR, Sakai RR. Androgenic influences on behavior, body weight, and body composition in a model of chronic social stress. Endocrinology. 2007;148(12):6145–56. [PubMed]
56. Tamashiro KLK, Nguyen MMN, Fujikawa T, Xu T, Ma LY, Woods SC, et al. Metabolic and endocrine consequences of social stress in a visible burrow system. Physiol & Behav. 2004;80:683–93. [PubMed]
57. Tamashiro KLK, Nguyen MMN, Ostrander MM, Gardner SR, Ma LY, Woods SC, et al. Social stress and recovery: implications for body weight and body composition. Am J Physiol Regul Integr Comp Physiol. 2007;293:R1864–R74. [PubMed]
58. Monder C, Sakai RR, Miroff Y, Blanchard DC, Blanchard RJ. Reciprocal changes in plasma corticosterone and testosterone in stressed male rats maintained in a visible burrow system: evidence for a mediating role of testicular 11 beta-hydroxysteroid dehydrogenase. Endocrinology. 1994 Mar;134(3):1193–8. [PubMed]
59. Blanchard DC, Cholvanich P, Blanchard RJ, Clow DW, Hammer RP, Jr, Rowlett JK, et al. Serotonin, but not dopamine, metabolites are increased in selected brain regions of subordinate male rats in a colony environment. Brain Res. 1991 Dec 24;568(1–2):61–6. [PubMed]
60. Mendelson S, McEwen BS. Autoradiographic analyses of the effects of restraint-induced stress on 5-HT1A, 5-HT1C and 5-HT2 receptors in the dorsal hippocampus of male and female rats. Neuroendo. 1991;54:454–61. [PubMed]
61. Watanabe Y, Sakai R, McEwen BS, Mendelson S. Stress and antidepressant effects on hippocampal and cortical 5-HT1A and 5-HT2 receptors and transport sites for serotonin. Brain Res. 1993;615:87–94. [PubMed]
62. Torda T, Murgas K, Cechova E, Kiss A, Saavedra J. Adrenergic regulation of [3H]ketanserin binding sites during immobilization stress in the rat frontal cortex. Brain Res. 1990;527:198–203. [PubMed]
63. McKittrick CR, Blanchard DC, Blanchard RJ, McEwen BS, Sakai RR. Serotonin receptor binding in a colony model of chronic social stress. Biological Psychiatry. 1995;37:383–93. [PubMed]
64. Watanabe Y, McKittrick CR, Blanchard DC, Blanchard RJ, McEwen BS, Sakai RR. Effects of chronic social stress on tyrosine hydroxylase mRNA and protein levels. Mol Brain Res. 1995;32:176–80. [PubMed]
65. Lucas LR, Celen Z, Tamashiro KLK, Blanchard RJ, Blanchard DC, Markham C, et al. Repeated exposure to social stress has long-term effects on indirect markers of dopaminergic activity in brain regions associated with motivated behavior. Neuroscience. 2004;124:449–57. [PubMed]
66. Piazza PV, Le Moal ML. Pathophysiological basis of vulnerability to drug abuse: role of an interaction between stress, glucocorticoids, and dopaminergic neurons. Annu Rev Pharmacol Toxicol. 1996;36:359–78. [PubMed]
67. Kozorovitskiy Y, Gould E. Dominance hierarchy influences adult neurogenesis in the dentate gyrus. J Neurosci. 2004 Jul 28;24(30):6755–9. [PubMed]
68. McEwen BS. Stress, sex, and neural adaptation to a changing environment: mechanisms of neuronal remodeling. Ann N Y Acad Sci. 2010 Sep;1204(Suppl):E38–59. Epub 2010/09/25. eng. [PMC free article] [PubMed]
69. Blanchard DC, Spencer RL, Weiss SM, Blanchard RJ, McEwen B, Sakai RR. Visible burrow system as a model of chronic social stress: behavioral and neuroendocrine correlates. Psychoneuroendocrinology. 1995;20(2):117–34. [PubMed]
70. Woods SC, Seeley RJ, Porte D, Jr, Schwartz MW. Signals that regulate food intake and energy homeostasis. Science. 1998 May 29;280(5368):1378–83. [PubMed]
71. Tamashiro KLK, Hegeman MA, Sakai RR. Chronic social stress in a changing dietary environment. Physiol & Behav. 2006;89:536–42. [PubMed]
72. Melhorn SJ, Krause EG, Scott KA, Mooney MR, Johnson JD, Woods SC, et al. Meal patterns and hypothalamic NPY expression during chronic social stress and recovery. Am J Physiol Regul Integr Comp Physiol. 2010 Sep;299(3):R813–22. [PubMed]
73. Scott KA, Melhorn SJ, Sakai RR. Effects of Chronic Social Stress on Obesity. Current obesity reports. 2012 Mar;1(1):16–25. [PMC free article] [PubMed]
74. Roth JD, Hughes H, Kendall E, Baron AD, Anderson CM. Antiobesity effects of the beta-cell hormone amylin in diet-induced obese rats: effects on food intake, body weight, composition, energy expenditure, and gene expression. Endocrinology. 2006 Dec;147(12):5855–64. [PubMed]
75. Smeltzer M, Scott K, Melhorn S, Krause E, Sakai R. Amylin blunts hyperphagia and reduces weight and fat gain during recovery in socially stressed rats. Am J Physiol Regul Integr Comp Physiol. 2012 Sep 15;303(6):R676–82. [PubMed]
76. McEwen BS, Stellar E. Stress and the Individual: Mechanisms leading to disease. Archives of Internal Medicine. 1993;153:2093–101. [PubMed]
77. McEwen BS, Gianaros PJ. Stress- and allostasis-induced brain plasticity. Annu Rev Med. 2011 Feb 18;62:431–45. Epub 2010/08/17. eng. [PMC free article] [PubMed]
78. de Leon MJ, George AE, Golomb J, Tarshish C, Convit A, Kluger A, et al. Frequency of hippocampus atrophy in normal elderly and Alzheimer’s disease patients. Neurobiol Aging. 1997;18:1–11. [PubMed]
79. Gold SM, Dziobek I, Sweat V, Tirsi A, Rogers K, Bruehl H, et al. Hippocampal damage and memory impairments as possible early brain complications of type 2 diabetes. Diabetologia. 2007;50:711–9. [PubMed]
80. Sheline YI. Neuroimaging studies of mood disorder effects on the brain. Biol Psychiat. 2003;54:338–52. [PubMed]
81. Starkman MN, Giordani B, Gebrski SS, Berent S, Schork MA, Schteingart DE. Decrease in cortisol reverses human hippocampal atrophy following treatment of Cushing’s disease. Biol Psychiat. 1999;46:1595–602. [PubMed]
82. Gurvits TV, Shenton ME, Hokama H, Ohta H, Lasko NB, Gilbertson MW, et al. Magnetic resonance imaging study of hippocampal volume in chronic, combat-related posttraumatic stress disorder. Biol Psychiatry. 1996;40:1091–9. [PMC free article] [PubMed]
83. Gianaros PJ, Jennings JR, Sheu LK, Greer PJ, Kuller LH, Matthews KA. Prospective reports of chronic life stress predict decreased grey matter volume in the hippocampus. NeuroImage. 2007;35:795–803. [PMC free article] [PubMed]
84. Marsland AL, Gianaros PJ, Abramowitch SM, Manuck SB, Hariri AR. Interleukin-6 covaries inversely with hippocampal grey matter volume in middle-aged adults. Biol Psychiat. 2008;64:484–90. [PMC free article] [PubMed]
85. Erickson KI, Prakash RS, Voss MW, Chaddock L, Hu L, Morris KS, et al. Aerobic fitness is associated with hippocampal volume in elderly humans. Hippocampus. 2009 Oct;19(10):1030–9. Epub 2009/01/06. eng. [PMC free article] [PubMed]
86. Cho K. Chronic ‘jet lag’ produces temporal lobe atrophy and spatial cognitive deficits. Nature Neurosci. 2001;4:567–8. [PubMed]
87. Stockmeier CA, Mahajan GJ, Konick LC, Overholser JC, Jurjus GJ, Meltzer HY, et al. Cellular changes in the postmortem hippocampus in major depression. Biol Psychiat. 2004;56:640–50. [PMC free article] [PubMed]
88. McEwen BS. Physiology and neurobiology of stress and adaptation: Central role of the brain. Physiol Rev. 2007;87:873–904. [PubMed]
89. Adler NE, Boyce TW, Chesney MA, Folkman S, Syme L. Socioeconomic Inequalities in Health. JAMA. 1993;269(24):3140–5. [PubMed]
90. Brunner EJ, Marmot MG, Nanchahal K, Shipley MJ, Stansfeld SA, Juneja M, et al. Social inequality in coronary risk: central obesity and the metabolic syndrome. Evidence from the Whitehall II study. Diabetologia. 1997;40:1341–9. [PubMed]
91. Yau PL, Castro MG, Tagani A, Tsui WH, Convit A. Obesity and metabolic syndrome and functional and structural brain impairments in adolescence. Pediatrics. 2012 Oct;130(4):e856–64. Epub 2012/09/05. eng. [PMC free article] [PubMed]
92. Gianaros PJ, Horenstein JA, Cohen S, Matthews KA, Brown SM, Flory JD, et al. Perigenual anterior cingulate morphology covaries with perceived social standing. SCAN. 2007;2007:1–13. [PMC free article] [PubMed]
93. Gianaros PJ, Marsland AL, Sheu LK, Erickson KI, Verstynen TD. Inflammatory pathways link socioeconomic inequalities to white matter architecture. Cereb Cortex. 2013 Sep;23(9):2058–71. [PMC free article] [PubMed]
94. Verstynen TD, Weinstein A, Erickson KI, Sheu LK, Marsland AL, Gianaros PJ. Competing physiological pathways link individual differences in weight and abdominal adiposity to white matter microstructure. Neuroimage. 2013 Oct;79:129–37. Epub 2013/05/04. eng. [PMC free article] [PubMed]
95. Halfon N, Larson K, Lu M, Tullis E, Russ S. Lifecourse health development: past, present and future. Maternal and child health journal. 2014 Feb;18(2):344–65. [PMC free article] [PubMed]
96. Liston C, McEwen BS, Casey BJ. Psychosocial stress reversibly disrupts prefrontal processing and attentional control. Proc Natl Acad Sci USA. 2009;106:912–7. [PubMed]
97. Liston C, Miller MM, Goldwater DS, Radley JJ, Rocher AB, Hof PR, et al. Stress-induced alterations in prefrontal cortical dendritic morphology predict selective impairments in perceptual attentional set-shifting. J Neurosci. 2006;26:7870–4. [PubMed]
98. Radley JJ, Rocher AB, Janssen WGM, Hof PR, McEwen BS, Morrison JH. Reversibility of apical dendritic retraction in the rat medial prefrontal cortex following repeated stress. Exper Neurol. 2005;196:199–203. [PubMed]
99. Bloss EB, Janssen WG, McEwen BS, Morrison JH. Interactive effects of stress and aging on structural plasticity in the prefrontal cortex. J Neurosci. 2010 May 12;30(19):6726–31. Epub 2010/05/14. eng. [PMC free article] [PubMed]
100. Karatsoreos IN, Bhagat S, Bloss EB, Morrison JH, McEwen BS. Disruption of circadian clocks has ramifications for metabolism, brain, and behavior. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1657–62. [PubMed]
101. Rao RP, Anilkumar S, McEwen BS, Chattarji S. Glucocorticoids protect against the delayed behavioral and cellular effects of acute stress on the amygdala. Biol Psychiatry. 2012 Sep 15;72(6):466–75. Epub 2012/05/11. eng. [PMC free article] [PubMed]
102. Drevets WC, Raichle ME. Neuroanatomical Circuits in Depression: Implications for Treatment mechanisms. Psychopharmacology Bulletin. 1992;28:261–74. [PubMed]
103. Lupien SJ, Parent S, Evans AC, Tremblay RE, Zelazo PD, Corbo V, et al. Larger amygdala but no change in hippocampal volume in 10-year-old children exposed to maternal depressive symptomatology since birth. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14324–9. Epub 2011/08/17. eng. [PubMed]
104. Starkman MN, Gebarski SS, Berent S, Schteingart DE. Hippocampal formation volume, memory dysfunction, and cortisol levels in partiens with Cushing’s syndrome. Biol Psychiatry. 1992;32:756–65. [PubMed]
105. Convit A, Wolf OT, Tarshish C, de Leon MJ. Reduced glucose tolerance is associated with poor memory performance and hippocampal atrophy among normal elderly. Proc Natl Acad Sci USA. 2003;100:2019–22. [PubMed]
106. Vermetten E, Vythilingam M, Southwick SM, Charney Ds, Bremner JD. Long-term treatment with paroxetine increases verbal declarative memory and hippocampal volume in posttraumatic stress disorder. biol Psychiat. 2003;54:693–702. [PMC free article] [PubMed]
107. Erickson KI, Voss MW, Prakash RS, Basak C, Szabo A, Chaddock L, et al. Exercise training increases size of hippocampus and improves memory. Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3017–22. Epub 2011/02/02. eng. [PubMed]
108. Holzel BK, Carmody J, Evans KC, Hoge EA, Dusek JA, Morgan L, et al. Stress reduction correlates with structural changes in the amygdala. Soc Cogn Affect Neurosci. 2010 Mar;5(1):11–7. Epub 2009/09/25. eng. [PMC free article] [PubMed]