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Logo of jbcThe Journal of Biological Chemistry
 
From:
Published online 2015 November 30. doi: 10.1074/jbc.M115.664292

TABLE 2

Co- and post-translational solubilization and activity of CF expressed MraY homologues

ProteinMode1st compound2nd compoundSolubilityLipid IStabilitya
%nmolg4 °C
Hp-MraYP-CFLMPG (1)-100(+)blow
DDM (1)-10(+)blow
LMPG (0.5)/DDM (1)-100(+)blow
LMPG (0.75)DDM (0.2)100c-
DPC (2)DDM (0.2)100c-
LS (0.8)DDM (0.2)95c-
D-CFDigitonin (0.4)DDM (0.2)100c-
Brij35 (0.4)DDM (0.2)100c-
Brij78 (0.4)DDM (0.2)100c-
L-CFND (DMPG)-909.7c1 week
ND (DMPC)-90c-
Bp-MraYP-CFLMPG (1)-100b-
DDM (1)-10b-
LMPG (0.5)/DDM (1)-100b-
D-CFDigitonin (0.4)DDM (0.2)20c-
Brij35 (0.4)DDM (0.2)95c-
Brij78 (0.4)DDM (0.2)80c-
L-CFND (DMPG)-602.3c1 week
ND (DMPC)-90c-
Bb-MraYP-CFLMPG (1)-100b-
DDM (1)-10b-
D-CFDigitonin (0.4)DDM (0.2)40c-
Brij35 (0.4)DDM (0.2)100c-
Brij78 (0.4)DDM (0.2)95c-
L-CFND (DMPG)-80c-
Cp-MraYD-CFDigitonin (0.4)DDM (0.2)40c-
Brij35 (0.4)DDM (0.2)70c-
Brij78 (0.4)DDM (0.2)70c-
L-CFND (DMPG)-7010.8c1–3 days

a Lipid I formation after storage at 4 °C for the indicated period.

b Without purification (+): activity was unstable and only detectable in the reaction mixture.

c Samples after affinity purification.