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Logo of jbcThe Journal of Biological Chemistry
 
From:
Published online 2015 November 30. doi: 10.1074/jbc.M115.664292

FIGURE 4.

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Lipid I and II formation with cell-free expressed Mur enzymes and Bs-MraY·nanodisc (DMPG) complexes. A, SDS-PAGE analysis of purified soluble Mur proteins. Samples were separated by 11% Tricine gels and stained with Coomassie Blue. B, TLC detection of lipid I and lipid II formation by cell-free expressed Mur enzymes and Bs-MraY·nanodisc (DMPG) complexes. Similar results were obtained in corresponding combinations with cell-free expressed Ec-MraY·nanodisc (DMPG) complexes (data not shown). As controls, in vivo expressed Mur enzymes of S. aureus and cell-free expressed Bs-MraY·nanodisc (DMPG) complexes were used for lipid I and lipid II formation.

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