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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Cell Physiol. Author manuscript; available in PMC 2016 July 1.
Published in final edited form as:
PMCID: PMC4725317
NIHMSID: NIHMS751994

Role of the Unfolded Protein Response, GRP78 and GRP94 in Organ Homeostasis

Abstract

The endoplasmic reticulum (ER) is a cellular organelle where secretory and membrane proteins, as well as lipids, are synthesized and modified. When cells are subjected to ER stress, an adaptive mechanism referred to as the Unfolded Protein Response (UPR) is triggered to allow the cells to restore homeostasis. Evidence has accumulated that the UPR pathways provide specialized and unique roles in diverse development and metabolic processes. The glucose regulated proteins (GRPs) are traditionally regarded as ER proteins with chaperone and calcium binding properties. The GRPs are constitutively expressed at basal levels in all organs, and as stress-inducible ER chaperones, they are major players in protein folding, assembly and degradation. This conventional concept is augmented by recent discoveries that GRPs can be actively translocated to other cellular locations such as the cell surface, where they assume novel functions that regulate signaling, proliferation, apoptosis and immunity. Recent construction and characterization of mouse models where the gene encoding for the UPR components and the GRPs is genetically altered provide new insights on the physiological contribution of these proteins in vivo. This review highlights recent progress towards the understanding of the role of the UPR and two major GRPs (GRP78 and GRP94) in regulating homeostasis of organs arising from the endoderm, mesoderm and ectoderm. GRP78 and GRP94 exhibit shared and unique functions, and in specific organs their depletion elicits adaptive responses with physiological consequences.

Keywords: Endoplasmic reticulum, GRP78/BiP, GRP94, UPR, Mouse models, Organs, Cancer

Since the initial discovery of the glucose regulated proteins (GRPs) about 40 years ago, the knowledge of these proteins has undergone rapid expansion after they have been identified as stress-inducible molecular chaperones belonging to the heat shock protein (HSP) family (Lee, 2001). In contrast to cytosolic HSPs, the GRPs contain signal peptides that target them to the endoplasmic reticulum (ER) where they perform critical functions in maintaining ER homeostasis and assist in protein folding and degradation (Ma and Hendershot, 2004; Ni and Lee, 2007; Marzec et al., 2012). The recent discovery that these GRPs can localize to the cell surface and regulate novel functions beyond the ER adds to the importance of these proteins in health and disease (Gonzalez-Gronow et al., 2009; Luo and Lee, 2013; Ni et al., 2011). Additionally, the unfolded protein response (UPR), which allows the cells to adapt to ER stress, plays a major role in maintaining ER function when the protein production exceeds the folding capacity of the ER. This review focuses on the contributions of the UPR and the GRPs in regulating organ homeostasis and integrity, and summarizes important insights derived from conventional and tissue specific knockout mouse models.

Basic Functions of the GRPs

The ER is a specialized perinuclear organelle where secretory and membrane proteins, as well as lipids, are synthesized. Within the lumen of the ER, protein chaperones assist in folding of newly synthesized polypeptides and prevent aggregation of unfolded or misfolded proteins (Ni and Lee, 2007). Quality control exists in the ER to avoid accumulation of unfolded or misfolded proteins. Abnormal protein conformations are a major cause for disturbed cellular homeostasis; therefore, perturbations in the ER are thought to be the origin of many diseases and developmental abnormalities (Schroder and Kaufman, 2005). ER stress occurs when there is an excess influx of nascent and unfolded polypeptides or the protein folding machinery is impaired. Signal transduction cascades, termed the UPR, are activated to restore the ER to its normal physiological state. The UPR turns on a cascade of pathways to restore homeostasis through transient arrest of protein translation, and at the same time, increases the folding capacity and decreases the burden of misfolded protein through degradation. However, if ER stress is too severe to be rescued, the UPR can also induce apoptotic pathways (Ron and Walter, 2007). In addition to secreted and membrane protein synthesis and secretion, the ER is a critical site for maintaining lipid metabolism, including lipid synthesis, modification and export (Fu et al., 2012).

The most abundant ER chaperone is the 78-kilodalton glucose regulated protein (GRP78/BiP), which is responsible for maintaining the permeability barrier of the ER during protein translocation, guiding protein folding and assembly, and targeting misfolded proteins for degradation (Ma and Hendershot, 2004). GRP78 is also an ER calcium binding protein. Importantly, GRP78 is a gatekeeper of the mammalian UPR. In non-stressed cells, GRP78 associates with the transmembrane ER stress sensors inositol-requiring kinase 1 (IRE1), activating transcription factor 6 (ATF6) and PKR-like eukaryotic initiation factor 2α kinase (PERK). Upon stress, malfolded proteins titrate GRP78 away from associations with these proteins, freeing them for activation of the UPR (Rutkowski and Kaufman, 2004). The UPR can also be triggered when GRP78 is knockdown in non-stressed mammalian cells, confirming that GRP78 is a negative regulator of the UPR in multiple models (Pyrko et al., 2007; Li et al., 2008).

Analogous to its suppressing activity of the UPR, GRP78 also acts as a potent suppressor of apoptosis through repressing the activation of the pro-apoptotic machinery (such as BIK and caspase-7) localized to the outer surface of the ER (Reddy et al., 2003; Fu et al., 2007; Zhou et al., 2011). Upon titration of GRP78, not only is the pro-apoptotic CHOP being induced as a consequence of UPR activation, intrinsic apoptosis is also triggered (Lee, 2014). GRP78 maintains ER integrity. GRP78 depletion leads to ER expansion and failure to form autophagosomes, therefore suppressing stress-induced autophagy (Li et al., 2008). Furthermore, it is now established that GRP78 can be relocalized to the cell surface, as well as associated with the mitochondria and nucleus, and even being actively secreted in specific cell lines (Ni et al., 2011). In these new cellular localizations, GRP78 assumes additional functions that control signaling, proliferation, invasion, apoptosis, inflammation and immunity (Lee, 2014). Of particular interest is that cell surface GRP78 is both an upstream regulator and downstream target of the PI3K/AKT pathway, which is important for development and tumorigenesis (Shani et al., 2008; Gray et al., 2013; Liu et al., 2013b; Zhang et al., 2013).

Another major ER chaperone is the 94-kilodalton glucose regulated protein (GRP94/Gp96), which is the most abundant glycoprotein in the ER. Interestingly, unlike GRP78 which is conserved from yeast to human, GRP94 exists only in multi-cellular organisms. GRP94 is instrumental in the initiation of both the innate and adaptive immune response (Yang and Li, 2005). GRP94, like GRP78, participates in protein folding, interacts with other components of the ER protein folding machinery, stores ER calcium and assists in the targeting of misfolded proteins for ER associated degradation (ERAD) (Eletto et al., 2010; Marzec et al., 2012). Compared to GRP78, the client proteins of GRP94 are more selective with critical roles in immunity, growth signaling and cell adhesion. Examples of GRP94 client proteins include histocompatibility class I (MHC I), insulin-like growth factor II (IGF II), Toll like receptor I (TLR1) and a subset of integrins (Liu and Li, 2008; Eletto et al., 2010; Staron et al., 2010). GRP94 also exhibits cell surface and secreted forms that facilitate antigen presentation and immune responses (Luo and Lee, 2013).

With the creation and characterization of conventional and conditional knockout models, the role of UPR, GRP78 and GRP94 in mouse embryonic development and adult organ homeostasis and integrity can be directly investigated. Below, the major findings from these studies are discussed and the phenotypes of each mouse model are summarized in Table 1 for the UPR components, Table 2 for GRP78 and Table 3 for GRP94.

Table 1
Mutant mouse models of genes encoding UPR components
Table 2
GRP78 mutant mouse models
Table 3
GRP94 mutant mouse models

Whole Body

The essential role of GRP78 in cell proliferation and survival was first demonstrated in 2006 in a mouse model where both Grp78 alleles were deleted. The Grp78−/− embryos failed to survive beyond the peri-implantation stage (around E3.5), associating with reduced proliferation and massive apoptosis of the inner cell mass, highlighting the fact that no other protein can substitute for the function of GRP78 (Luo et al., 2006). In contrast, Grp78 heterozygous (Grp78+/−) mice, which express half of the wild-type level of the GRP78 protein, are viable and demonstrate normal organ development. Furthermore, through compensatory mechanisms in response to partial loss of GRP78, Grp78 heterozygosity protects against high fat diet (HFD)-induced obesity and insulin resistance, as well as cerulein-induced acute pancreatitis in the exocrine pancreas of HFD fed mice (Ye et al., 2010a; Ye et al., 2010b).

Grp94−/− embryos survived up to E8.5, while Grp94+/− pups were born in expected Mendelian ratio and displayed normal development (Wanderling et al., 2007; Mao et al., 2010). The survival of Grp94−/− embryos past the blastocyst stage allows for the establishment of Grp94−/− embryonic stem cell (ESC) lines. Nonetheless, since GRP94 is important for IGF-1 maturation and secretion, the growth of these ESCs exhibit dependence on exogenous growth factors. Whereas Grp94−/− ESCs can differentiate into cells of all three germ layers: ectoderm (neurons), mesoderm (adipocytes) and endoderm (hepatocytes), they cannot differentiate into cardiomyocyte-like cells (Wanderling et al., 2007; Mao et al., 2010).

Liver

The liver is a central organ for whole body homeostasis, including carbohydrate metabolism, glycogen storage, biosynthesis of amino acids and nucleotides, production of plasma proteins and hormones, and lipid metabolism (Tanaka et al., 2011). The levels of triglycerides in the liver are regulated by diverse mechanisms such as de novo lipid synthesis, lipolysis, dietary lipid uptake, and delivery/secretion of lipoprotein particles (Jo et al., 2013). As noted earlier, ER is the primary organelle for lipid metabolism, due to the residency of many enzymes involved in intermediary and complex lipid metabolism (Fu et al., 2012). ER stress and UPR signaling have been well established to link to the excessive accumulation of triglycerides in the liver, which is termed as hepatic steatosis (Fu et al., 2012). ER stress in liver can be induced by oxidative stress, chemical toxicity, hepatic viral infection, metabolic disorders, and abuse of drugs and alcohol (Liu et al., 2010). When the ER cannot restore its functionality in a timely manner under these conditions, it enters into a pathological state, perturbing the balance of lipid homeostasis. Some major components of the UPR regulate lipid metabolism in liver. For example, conditional knockout of Xbp1 in the liver led to marked hypocholesterolemia and hypotriglyceridemia, as a result of decreased production of lipids in the liver (Lee et al., 2008). When mice received intraperitoneal injection of the ER-stress inducing reagent tunicamycin, wild-type mice were able to recover from the insult, whereas ATF6α−/− mice accumulated neutral lipids in the liver and developed steatosis (Yamamoto et al., 2010). Liver specific PERK-null mice showed impaired UPR activation following tunicamycin-injection (Bunpo et al., 2009).

As expected from the generally protective role of GRP78, liver-specific Grp78 knockout mice (Grp78f/f; Alb-Cre) exhibited mild fatty liver and liver injury, as well as UPR activation (Ji et al., 2011; Chen et al., 2014c). However, the hepatocytes of the Grp78f/f; Alb-Cre mice still expressed about 30% of wild-type GRP78 level even at 6 months of age, and the ductal cells always maintained GRP78 expression. At around 14 months, the surface of Grp78f/f; Alb-Cre livers displayed some small benign nodules showing fat accumulation accompanied by inflammation (Chen et al., 2014c). GRP78 deficiency in liver exacerbated liver injury induced by alcohol, HFD, drugs and toxins, which could be partially alleviated by simultaneous treatment of the chemical chaperone 4-phenylbutyrate (4-PBA) (Ji et al., 2011). For male Grp78f/f; Alb-Cre mice, no sign of malignancy was evident in aged mice (Chen et al., 2014c). However, it was reported that older, long-term alcohol-fed female Grp78 liver knockout mice showed alterations in DNA methylation and ERAD gene expression, with appearance of tumor-like masses in the liver (Han et al., 2013). In delineating the progression of hepatocellular carcinoma (HCC) which often arises from liver injury, recent experiments on mouse tumor models revealed both tumor suppressing and promoting roles in the same molecule (Feng, 2012). In the context of double knockout of GRP78 and the tumor suppressor PTEN in the liver, increased hepatomegaly, steatosis, liver injury and progenitor cell proliferation, and acceleration of both HCC and cholangiocarcinoma (CC) were observed, compared to the single PTEN knockout mice (Chen et al., 2014c). Nonetheless, GRP78 is unlikely a tumor suppressor since all the HCC and CC in these mice showed strong re-expression of GRP78. Rather, GRP78 appears to be required for promotion of liver tumorigenesis, as it is also highly elevated in human liver cancer (Lim et al., 2005).

Depletion of GRP94 in the liver by Alb-Cre was more efficient compared to GRP78. Livers of the young Grp94f/f; Alb-Cre mice showed minor liver injury and focal steatosis, but strikingly, liver progenitor cells expansion and disorganization of cell adhesion molecules were evident, which is consistent with the role of GRP94 in maintaining cell-cell interaction (Chen et al., 2014b). Grp94f/f; Alb-Cre livers developed abnormal small nodules at 15 months, and HCC and ductular reactions (DRs) by 21 months (Chen et al., 2014a). Strikingly, Grp94f/f; Alb-Cre livers were gradually repopulated with GRP94-positive hepatocytes, while the DRs maintained GRP94-negative (Chen et al., 2014a). In the context of double knockout of GRP94 and PTEN in the liver, accelerated development of HCC and CC was observed, with selective activation of the ERK pathway, disruption of cell adhesion and activation of liver progenitor cells at the premalignant stage (Chen et al., 2014b). Nonetheless, heterogeneous GRP94 expression from very low to wild-type level was observed in the HCC formed in the double GRP94 and PTEN knockout mice, whereas the CC cells remained mostly GRP94 negative (Lee and Chen, 2014). Thus, while GRP94 maintains cell adhesion and stem cell quiescence which are tumor suppressing factors, it can also support tumorigenesis as a stress chaperone and regulator of pro-oncogenic signaling pathways. Recently, in another model of liver specific knockout of GRP94, elevation of long chain ceramides and upregulation of several enzymes in the biogenesis of ceramides were observed, indicating a novel role of GRP94 in sphingolipid homeostasis (Rachidi et al., 2014). This same study also showed repopulation of the liver with GRP94-positive hepatocytes as the mice aged, and upon treatment with carcinogen, the knockout mice developed hepatic hyperplasia and cancer that were GRP94-positive.

Pancreas

The bulk of the pancreas is composed of exocrine tissue, which is highly specialized for the production and secretion of digestive enzymes. In response to meal stimulation, acinar cells of the exocrine pancreas exhibit the highest protein synthesis rate among human tissues (Case, 1978). Acinar cells adapt to these functions by expansion of the ER. Additionally, ER stress caused by misfolding of mutant digestive enzymes has been linked to hereditary chronic pancreatitis in humans, suggesting that the UPR and ER chaperones are important for maintaining pancreatic homeostasis (Kereszturi et al., 2009). In agreement with this, inducible conditional knockout of Perk in adult pancreata resulted in hyperglycemia associated with loss of islet and β−cell architecture, intracellular accumulation of proinsulin and Glut 2, massive ER expansion and onset of adaptive UPR in pancreatic tissue (Gao et al., 2012). Thus, PERK is essential for secretory homeostasis and β-cell survival in adult mice, implying that development of PERK inhibitor in human subjects needs to be cautiously pursued (Atkins et al., 2013). Diet-induced obese ATF6α−/− mice exhibited glucose intolerance due to pancreatic β-cell failure, associating with dilated ER, but are partially resistant to diet-induced insulin resistance (Usui et al., 2012). Conditional knockout of XBP1 in adult pancreatic acinar cells induced extensive apoptosis, followed by pancreas regeneration of acinar cells from the surviving XBP1 positive cells (Hess et al., 2011).

For ER chaperones, Grp78+/− acinar cells exhibited partial ER dilation and fared worse in cerulein-induced pancreatitis associated with CHOP induction (Ye et al., 2010b). Interestingly, in HFD-fed mice, Grp78 heterozygosity triggered compensatory upregulation of other ER chaperones in the pancreas, corresponding with mitigated pancreatitis upon cerulein treatment. However, these responses are dependent on the genetic background of the mice, underscoring the complexity of these physiological adaptations. In human and murine models of pancreatic cancer, GRP78 expression is highly elevated in the ductal structures of pre-cancer, as well as in pancreatic adenocarcinoma (PDAC) (Hill et al., 2012). In the mouse PDAC lesions, GRP78 is present in both the cytoplasm and the membranes, where it co-localized with activated AKT. These studies suggest that GRP78 could have the dual function of maintaining ER integrity as well as mediating PI3K/AKT oncogenic signaling at the cell surface in pancreatic cancer.

Intestine and Esophagus

The mammalian intestine is covered by a single layer of epithelial cells that are replenished every 4-5 days through proliferation and differentiation of intestinal stem cells within the intestinal crypts (van der Flier and Clevers, 2009). The interplay between ER stress and inflammation contribute to the pathogenesis of intestinal diseases (Guo and Li, 2014). XBP1 deletion in intestinal epithelial cells results in UPR activation, spontaneous enteritis and increased susceptibility to induced colitis (Kaser et al., 2008). Abrogation of Wnt signaling resulted in loss of proliferation, whereas activation of Wnt pathway led to the phenotypes associated with early colorectal lesions (Kuhnert et al., 2004; Sansom et al., 2004). Mouse models harboring intestinal knockout of GRP94 led to Wnt signaling defect through loss of the Wnt co-receptor LRP6, postnatal death, loss of intestinal barrier function, decreased number of villi and significant reduction of crypt (Liu et al., 2013a). The rescue of these phenotypes via β-catenin activation indicated a critical role of GRP94 in regulation of canonical Wnt-signaling in intestine. In another study, Ah1-Cre mediated GRP78 knockout in intestine led to induction of ER stress, loss of self-renewal capacity and rapid repopulation of wild-type stem cells (Heijmans et al., 2013). This same study also showed Ah1-Cre activity in esophagus, and GRP78 null esophageal epithelial cells lost their proliferative capacity and were replaced by unrecombined GRP78-positive cells (Rosekrans et al., 2014).

Hematopoietic System

Hematopoiesis, which occurs during embryo development and adulthood, is the formation of various lineages of mature blood cells derived from hematopoietic stem cells (HSC) with self-renewal capability (Doulatov et al., 2012). Complex and dynamic molecular crosstalk occurs between HSCs and the niche cells, which involves secreted ligands and their receptors, extracellular matrix proteins and adhesion molecules (Lo Celso and Scadden, 2011; Boisset et al., 2013). As a major organelle for synthesis and trafficking of these secretory and surface molecules, a well-organized and functional ER is critical for maintenance of HSCs homeostasis. IRE1α/XBP-1 axis has been shown to be involved in many aspects of the hematopoietic system. For example, XBP-1 is needed for transition of mature activated B cells to antibody secreting plasma cells, as well as the formation of dendritic cells (Reimold et al., 2001; Iwakoshi et al., 2007; Todd et al., 2009). Upon stress and damage, human HSCs pool maintains clonal integrity through PERK induced apoptosis of damaged cells, whereas downstream progenitors mount an adaptive response leading to their survival (van Galen et al., 2014).

As the immunoglobulin heavy chain binding protein BiP, GRP78 facilitates antibody production in plasma cells, and retains unassembled heavy chains in the ER (Hendershot et al., 1987). GRP78 is a proteolytic target of subtilase cytotoxin (SubAB), which blocks antibody secretion in the B cells as a result of the cleaved C-terminal fragment of GRP78 binding and retaining the light chains in the ER (Hu et al., 2009). Mx1-Cre mediated acute GRP78 ablation in the mouse bone marrow led to activation of the UPR and significant reduction of HSCs, common lymphoid and myeloid progenitors via a cell intrinsic effect, at least in part due to increased apoptosis (Wey et al., 2012a). Acute induction of Grp78 heterozygosity by Mx1-Cre is sufficient to suppress PTEN-null induced AKT/S6K activation in bone marrow cells and leukemogenesis, with minimal effect on normal hematopoiesis (Wey et al., 2012b). This effect is mediated at least in part through cell surface GRP78, since treatment with a monoclonal antibody targeting GRP78 also suppressed PTEN-null induced AKT activation and leukemic blast production in the bone marrow (Liu et al., 2013b). Acting through cell surface GRP78, Cripto maintains HSCs in the hypoxic-niche as an intermediary of the hypoxia-inducible factor-1α (Miharada et al., 2011). Furthermore, GRP78 secreted to extracellular environment induces cytokine secretion and displays immunomodulatory properties (Panayi and Corrigall, 2006; Li and Li, 2012).

Many client proteins of GRP94 are key players in immune responses, including hematopoietic system-specific integrins, as well as several TLRs (Liu and Li, 2008). GRP94 is required at defined stages of early T- and B-cell development and GRP94 deficient bone marrow chimeras exhibited increase in HSCs, associating with AKT activation (Staron et al., 2010; Luo et al., 2013). The expansion of the HSC pool could result from impaired interaction of HSCs and the niche, as GRP94-null primitive hematopoietic cells showed loss of integrin α4 expression, correlating with enhanced mobilization and compromised homing and lodging ability (Luo et al., 2011). Cell surface GRP94 has been shown to participate in innate immune responses through interaction with TLR2/TLR4 and in adaptive immune response by escorting GRP94-associated peptides into MHC class I cross-presentation pathway (Jockheck-Clark et al., 2010). Overexpression of cell surface GRP94 induced dendritic cell activation and spontaneous lupus-like autoimmune diseases (Liu et al., 2003). Recombinant GRP94 is able to activate macrophages (Huang et al., 2009), polymorphonuclear neutrophils and monocytes, suggesting a novel function of GRP94 as a danger signal to the innate immune system (Radsak et al., 2003).

Muscle

PERK is important in maintaining muscle calcium homeostasis such that muscle cells isolated from Perk−/− mice showed high levels of ER stress and disruption of the calcium signaling complex (Huang et al., 2006). GRP94 has been studied extensively in muscle physiology. GRP94 is essential for muscle differentiation such that Grp94−/− ESCs cannot differentiate into any of the muscle sublineages (Wanderling et al., 2007). In skeletal and cardiac muscle, GRP94 is completely contained within the sarcoplasmic reticulum lumen (Vitadello et al., 1998), and reduction in GRP94 levels in skeletal myoblasts leads to loss of myocyte fusion competence (Ostrovsky et al., 2010). GRP94 functions as a protective stress protein in muscle, such that GRP94 level is transiently increased in fibrillating atrial myocytes, and cell death of stressed cardiomyocytes can be rescued through overexpression of GRP94 (Vitadello et al., 2003). IGF-I and IGF-II are essential for development and growth of skeletal muscles and their production is dependent on GRP94. Ablation of GRP94 in striated muscle of Grp94f/f; MCK-Cre mice resulted in smaller skeletal muscles with decreased IGF contents, and growth defect associating with diminished circulating IGF-1 (Barton et al., 2012).

Adipose Tissue

In addition to regulating fat mass and nutrient homeostasis, adipocytes participate in the immune response, blood pressure control, bone mass regulation and reproductive function through synthesis and release of hormones (Rosen and Spiegelman, 2006). White adipose tissue (WAT) functions as an endocrine organ, as it integrates metabolic signals and regulates energy balance by secreting adipokines such as leptin, adiponectin, resistin, and tumor necrosis factor α (TNFα) (Rosen and Spiegelman, 2006). Specific functions of ER in adipocytes include adipokine synthesis and secretion, triglyceride (TG) droplet synthesis and cholesterol sensing via release of Sterol Regulatory Element-Binding Protein (SREBP) from the ER membrane to nucleus (Maxfield and Tabas, 2005; Rosen and MacDougald, 2006). All three branches of the UPR contribute to adipogenesis. 3T3-L1 cells with XBP1 or IRE1α knockdown demonstrated profound defects in adipogenesis, and only the spliced form of XBP1 can rescue the impairment (Sha et al., 2009). The differentiation of MEFs into adipocytes induced by transduction with Myc-SREBP1 was attenuated by loss of PERK, with reduced expression of lipogenic enzymes (Bobrovnikova-Marjon et al., 2008). In C3H10T1/2 cells induced to adipogenesis, reduction of ATF6α expression led to decrease of key adipogenic genes and reduced lipid accumulation (Lowe et al., 2012).

Knockdown of GRP78 significantly delayed adipocyte differentiation from MEFs or preadipocytes (Zhu et al., 2013). In contrast, Grp94−/− ESCs are capable of differentiating into adipocytes (Mao et al., 2010). Grp78f/f; Ap2-Cre mice with Cre-activity in adipocytes as well as in brain and bone, showed dramatic reduction in adipose tissue, growth retardation, bone reduction, early mortality and altered profile of glucose homeostasis and adipokine secretion (Zhu et al., 2013). It is notable that dyslipidemia, fatty liver and type 2 diabetes phenotypes observed in other lipodystrophic mouse models were absent in the Grp78f/f; Ap2-Cre mice. As mentioned above, Grp78 heterozygosity in a specific genetic background showed improvement of insulin sensitivity, and this was mediated by adaptive UPR in the WAT (Ye et al., 2010a).

Mammary Gland

The mammary gland is composed of different cell types, including epithelial cells that form a branched structure, adipocytes, vascular endothelial cells, fibroblasts and immune cells (Macias and Hinck, 2012). Mammary gland development and function are regulated under different and interrelated components, such as growth factors, hormones and the extracellular matrix (ECM). Postnatal growth of mammary gland is influenced by growth hormone (GH), estrogen and insulin-like growth factor-1 (IGF1) (Macias and Hinck, 2012). Mammary gland branching morphogenesis is partially dependent on the ECM, ECM receptors and ECM degrading enzymes, which affect cell survival, polarity, proliferation, differentiation and adhesion (Fata et al., 2004). It demands a highly functional ER to accommodate for the production of all these secreted and surface proteins. In support that the integrity of the PERK pathway is important for mammary homeostasis, targeted deletion of Perk in mammary epithelium inhibited the sustained expression of lipogenic enzymes, leading to altered lipid composition in milk (Bobrovnikova-Marjon et al., 2008). PERK also facilitates survival of ECM-detached mammary epithelial cells by autophagy induction, ATP production and an antioxidant response, which may enhance survival of ECM-detached tumor cells in ductal carcinomas in situ (DCIS) (Avivar-Valderas et al., 2011).

For ER chaperones, GRP78, but not GRP94, is required for mammary gland development. Intriguingly, GRP78 knockdown in the mammary epithelium using MMTV-Cre did not succeed, however, could be achieved in the isolated Grp78f/f epithelial cells in vitro via infection with adenovirus Cre (Zhu et al., 2014). GRP78 ablated mammary epithelial stem/progenitor cells failed to regenerate the mammary glands in deepithelialized recipient mice. GRP78 that translocates to cell surface contributes to the regulation of Cripto signaling in mammary stem cells (Kelber et al., 2009; Spike et al., 2014). Targeted perturbation of cell surface Cripto/GRP78 complex blocked Cripto-mediated regulation of downstream AKT and TGFβ signaling pathways (Kelber et al., 2009). Grp78 heterozygosity impeded transgene-induced mammary tumor development, with the tumors showing reduced proliferation, increased apoptosis, and dramatic reduction of tumor angiogenesis (Dong et al., 2008). Early-phase mammary tumor growth and metastatic growth of melanoma to the lung was suppressed in syngeneic tumor models using Grp78+/− host mice (Dong et al., 2011). Furthermore, endothelial cell-specific Grp78 heterozygous knockout mice (Grp78f/+; Tie2-Cre) showed profound reduction of tumor angiogenesis and metastatic lesions with minor effect on normal tissue microvessel density (Dong et al., 2011). Collectively, these models established that GRP78 is not only required for tumor progression, but also for tumor neovascularization.

Prostate

The prostate is a compound tubuloalveolar exocrine gland and the prostate epithelium is comprised of tall columnar cells and basal cells which are supported by a fibroelastic stroma containing randomly oriented smooth muscle bundles. Homozygous deletion of Grp78 specifically in prostate epithelium of Grp78f/f; Probasin-Cre mice did not affect postnatal prostate development or the fertility of the mouse (Fu et al., 2008). It is possible that the prostate epithelium function can be sustained by the low level of residual GRP78 in these cells, or these cells are being sufficiently replenished by non-recombined GRP78-positive stem cells with self-renewal properties. In contrast, in mouse models, Pten null prostate tumorigenesis and AKT activation are potently suppressed by targeted knockout of GRP78 in the prostate epithelium (Fu et al., 2008).

Cerebellum

Neurodegenerative diseases are often associated with dysfunction in protein quality control, leading to accumulation of misfolded proteins that triggers ER stress (Rao and Bredesen, 2004). The function and potential of GRP78 as a therapeutic target for neurodegenerative disorders have been previously reviewed (Muchowski and Wacker, 2005; Wang et al., 2009; Gorbatyuk and Gorbatyuk, 2013). ER protein quality control is critical for cerebellar Purkinje cell (PC) survival, and is linked to the Marinesco-Sjogren syndrome in humans (Anttonen et al., 2005). PCs in the cerebellum are a class of GABAergic neurons and they are necessary for controlling movements with precision and convey the entire output of the cerebellar cortex (Mizuhara et al., 2010; Heiney et al., 2014). In a mouse model where GRP78 was specifically eliminated in the PCs, reduction of cytosolic ubiquitin and prominent dilatation of the ER were observed, accompanied by the activation of the UPR and compensatory upregulation of other ER chaperones (Wang et al., 2010). These mice exhibited growth retardation, severe motor coordination defect and cerebellar atrophy. In contrast, PCs deficient in GRP94 showed none of these cerebellar degeneration phenotypes, underscoring the specific requirement of GRP78 for the integrity of this organ (Wang et al., 2010). In agreement with these findings, a knockin mouse model expressing heterozygous mutant GRP78 also revealed motor disabilities in aging and degeneration of some motoneurons in the spinal cord (Jin et al., 2014). In another model with knockin of a mutant Grp78 gene, the mice exhibited disordered cerebral cortex and cerebellum, and smaller brain (Mimura et al., 2008). In mice harboring a hypomorph mutant of GRP78, neonatal respiratory failure, abnormal cerebellum and decrease in axon projection were observed (Favero et al., 2013).

Conclusions

Taken together, the ER is a crucial command center through participation in diverse essential cellular activities and potential influences on function of various organs. Evidence is accumulating that the UPR modulates a wide variety of physiological pathways that are critical for specific organ function. ER chaperones including GRP78 and GRP94 play major roles in ER integrity and their functions could be tissue-specific. The mouse models elegantly demonstrate the consequences of their deficiency or altered function in mouse development, tissue differentiation and organ maintenance. One important aspect learned from these in vivo studies is that while normal organs including the endothelium are not majorly affected when GRP78 expression level is reduced by half or lower, the ability for cancers to grow and metastasize can be severely compromised. In some cancers, GRP94 is also pro-oncogenic. The addiction of tumors and other pathologies to high levels of GRPs provides a therapeutic window to target GRPs with minimal harm to normal organs. Despite these advances, there are substantial gaps in our knowledge on how the UPR pathways and the GRPs exert their physiological effects. For example, their interacting partners as well as the intermediary steps in specific tissues remain to be elucidated. Drugs specifically targeting the UPR and the GRPs need to be further developed and their efficacy validated in clinical trials. Nonetheless, the revelation of the general and specific functions of the UPR pathways and GRPs in organ maintenance and tumorigenesis will provide new insight in designing therapeutic agents to maximize the benefits while minimizing the undesirable side effects on normal organs.

Acknowledgments

Contract grant sponsor: National Institutes of Cancer and National Institutes of Aging; Contract grant numbers: R01-CA027607, P01-AG034906.

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