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Logo of jlrJournal of Lipid Research
 
J Lipid Res. 2015 November; 56(11): 2226.
PMCID: PMC4617409

Erratum

The authors of “Oxidation of 7-dehydrocholesterol and desmosterol by human cytochrome P450 46A1” (J. Lipid Res. 2014. 55: 1933–1943) have advised that there were several errors in this article. There was an error in calculating the Km values in Table 1 that led to subsequent errors in the kcat/Km values in the same table. The correct Km and kcat/Km values were calculated and are shown in the corrected Table 1.

TABLE 1.
Kinetic parameters measured for 7-dehydrocholesterol, desmosterol, and cholesterol

PAGE 1938:

“The calculated kcat and KM values for the formation of 24-hydroxy-7-dehydrocholesterol (from 7-dehydrocholesterol) were 24 ± 1 pmol nmol−1 min−1 and 2.8 ± 0.02 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).” should read, “The calculated kcat and KM values for the formation of 24-hydroxy-7-dehydrocholesterol (from 7-dehydrocholesterol) were 24 ± 1 pmol nmol−1 min−1 and 0.24 ± 0.008 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).”

“The kcat and KM values for 24S,25-epoxycholesterol (from desmosterol) were 33 ± 1 pmol nmol−1 min−1 and 9.4 ± 1 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).” should read, “The kcat and KM values for 24S,25-epoxycholesterol (from desmosterol) were 33 ± 1 pmol nmol−1 min−1 and 2.17 ± 0.28 μM, respectively (corrected for the binding to HPβCD) (Table 1, supplementary Fig. VI).”

PAGE 1939:

“The enzyme efficiency (kcat/Km) was similar for 7-dehydrocholesterol compared with cholesterol but 2–3 times higher than that of desmosterol.” should read, “The enzyme efficiency (kcat/Km) for 7-dehydrocholesterol was higher by two–three times compared to cholesterol and six–seven times higher than that of desmosterol.”

These errors do not affect the overall results or conclusions of this work.


Articles from Journal of Lipid Research are provided here courtesy of American Society for Biochemistry and Molecular Biology