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Dobutamine induces YAP and TEAD translocation from the nucleus and stabilization of Ocln at the cell membrane of HPAFII cells. (A) Confocal immunofluorescence microscopy showing YAP and Ocln distribution in HPAFII cells. Cells treated with 10 or 20 μM dobutamine for 24 h were similarly analyzed for YAP and Ocln cell distribution. Scale bar, 20 μm. (B) Confocal immunofluorescence microscopy showing TEAD and Ocln distribution in HPAFII cells with control buffer addition vs. 20 μM dobutamine for 24 h; scale bar, 20 μm. (C) Immunoblot analysis of total YAP in nuclear and cytoplasmic fractions of HPAF II cells as a consequence of 10 or 20 μM dobutamine (Dob) exposure. HDAC (histone deacetylase) and β-actin were used as loading controls for nuclear and cytoplasmic fractions, respectively. Quantification of YAP expression values are the mean of 3 independent experiments ± SEM; ***P < 0.001. (D) Immunoblot analysis of TEAD in the nuclear fractions of HPAF II cells as a consequence of 10 or 20 μM dobutamine exposure. Quantification of TEAD expression values are mean of 3 independent experiments ± SEM; *P < 0.05.