Mitophagy occurred in both WT and Parkin KO mice after APAP treatment.A, representative EM images are shown from WT and Parkin KO mice treated with APAP (500 mg/kg) with or without CQ (60 mg/kg) for 6 h. An enlarged image of mitophagosomes is shown from the dotted line boxed area (arrows, mitophagosomes; M, mitochondria; N, nucleus). B, quantification of EM images. Data shown are means ± S.E. (n ≥ 2 mice per group, at least 10 images quantified per group; *, p < 0.05, no significant differences between WT and Parkin KO mice). C, WT and Parkin KO mice were treated with 500 mg/kg APAP or saline control for 6 and 24 h, and total liver lysates were analyzed for mitochondrial protein degradation by Western blot. Gapdh was used as a loading control. Results for two individual mice are shown. D and E, densitometry quantification of Western blots for Tom20 and cyclophilin D. Data shown are means ± S.E. (n = 4 for each group, no significant differences among groups). Results were normalized to Gapdh.