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Logo of neuroscibullNeuroscience Bulletin
 
Neurosci Bull. 2010 June; 26(3): 188–196.
Published online 2010 June 3. doi:  10.1007/s12264-010-0150-x
PMCID: PMC4386992
NIHMSID: NIHMS675667

Language: English | Chinese

Effects of pentobarbital anesthesia on nociceptive processing in the medial and lateral pain pathways in rats

戊巴比妥钠麻醉对大鼠痛觉加工内外侧通路信息处理的影响

Abstract

Objective

To investigate the effects of pentobarbital anesthesia on nociceptive processing in the medial and lateral pain pathways.

Methods

Laser stimulation was employed to evoke nociceptive responses in rats under awake or anesthetic conditions. Pain-related neuronal activities were simultaneously recorded from the primary somatosensory cortex (SI), ventral posterolateral thalamus (VPL), anterior cingulate cortex (ACC), and medial dorsal thalamus (MD) with 4 eight-wire microelectrode arrays.

Results

Compared with the awake state, pentobarbital anesthesia significantly suppressed the neural activities induced by noxious laser stimulation. Meanwhile, the pain-evoked changes in the neuronal correlations between cortex and thalamus were suppressed in both medial and lateral pain pathways. In addition, the spontaneous firing rates in all the 4 areas were altered (including inhibition and excitation) under the condition of anesthesia.

Conclusion

The nociceptive processing in the brain can be dramatically changed by anesthesia, which indicates that there are considerable differences in the brain activities between awake and anesthetized states. It is better to employ awake animals for recording neural activity when investigating the sensory coding mechanisms, especially pain coding, in order to obtain data that precisely reflect the physiological state.

Keywords: anesthesia, laser, multiple single-unit recording

摘要

目的

探讨麻醉对大鼠痛觉加工内外侧通路信息处理的影响。

方法

利用清醒动物神经细胞群单位放电多通道同步记录技术, 在大鼠的初级躯体感觉皮层(SI)、 丘脑腹后外侧核(VPL)、 前扣带皮层(ACC)以及丘脑背内侧核(MD)埋置电极, 给予对侧足底伤害性激光刺激, 观察大鼠清醒状态下以及戊巴比妥钠麻醉状态下由激光刺激引发的各脑区神经活动的变化。

结果

与清醒状态相比, 戊巴比妥钠麻醉显著降低了伤害性激光刺激所引发的四个脑区神经元活动的增强, 同时也抑制了由疼痛引起的内、 外侧通路上脑区之间的同步电活动。 另外, 各脑区的自发放电频率也因麻醉而发生显著改变, 包括抑制和增强两种情况。

结论

麻醉能显著改变疼痛相关的神经活动, 表明大脑活动在麻醉与清醒状态下有着很大差别。 该结果提示, 在研究感觉神经编码尤其是痛觉编码时, 最好使用清醒动物记录神经活动, 以获得能真正反映生理状态的实验数据。

关键词: 麻醉, 激光, 多通道记录

Footnotes

These authors contributed equally to this work.

References

[1] Albe-Fessard D., Berkley K.J., Kruger L., Ralston H.J., Willis W.D., Jr Diencephalic mechanisms of pain sensation. Brain Res. 1985;356:217–296. [PubMed]
[2] Hudson A.J. Pain perception and response: central nervous system mechanisms. Can J Neurol Sci. 2000;27:2–16. [PubMed]
[3] Schnitzler A., Ploner M. Neurophysiology and functional neuroanatomy of pain perception. J Clin Neurophysiol. 2000;17:592–603. doi: 10.1097/00004691-200011000-00005. [PubMed] [Cross Ref]
[4] Treede R.D., Kenshalo D.R., Gracely R.H., Jones A.K. The cortical representation of pain. Pain. 1999;79:105–111. doi: 10.1016/S0304-3959(98)00184-5. [PubMed] [Cross Ref]
[5] Shigenaga Y., Inoki R. Effects of morphine and barbiturate on the SI and SII potentials evoked by tooth pulp stimulation of rats. Eur J Pharmacol. 1976;36:347–353. doi: 10.1016/0014-2999(76)90088-1. [PubMed] [Cross Ref]
[6] Chapin J.K., Waterhouse B.D., Woodward D.J. Differences in cutaneous sensory response properties of single somatosensory cortical neurons in awake and halothane anesthetized rats. Brain Res Bull. 1981;6:63–70. doi: 10.1016/S0361-9230(81)80069-X. [PubMed] [Cross Ref]
[7] Lu H., Xu T.L. The general anesthetic pentobarbital slows desensitization and deactivation of the glycine receptor in the rat spinal dorsal horn neurons. J Biol Chem. 2002;277:41369–41378. doi: 10.1074/jbc.M206768200. [PubMed] [Cross Ref]
[8] Jackson M.F., Joo D.T., Al-Mahrouki A.A., Orser B.A., MacDonald J.F. Desensitization of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptors facilitates use-dependent inhibition by pentobarbital. Mol Pharmacol. 2003;64:395–406. doi: 10.1124/mol.64.2.395. [PubMed] [Cross Ref]
[9] Wan X., Mathers D.A., Puil E. Pentobarbital modulates intrinsic and GABA-receptor conductances in thalamocortical inhibition. Neuroscience. 2003;121:947–958. doi: 10.1016/j.neuroscience.2003.07.002. [PubMed] [Cross Ref]
[10] Shih Y.Y., Chang C., Chen J.C., Jaw F.S. BOLD fMRI mapping of brain responses to nociceptive stimuli in rats under ketamine anesthesia. Med Eng Phys. 2008;30:953–958. doi: 10.1016/j.medengphy.2007.12.004. [PubMed] [Cross Ref]
[11] Shaw F.Z., Chen R.F., Yen C.T. Dynamic changes of touch- and laser heat-evoked field potentials of primary somatosensory cortex in awake and pentobarbital-anesthetized rats. Brain Res. 2001;911:105–115. doi: 10.1016/S0006-8993(01)02686-5. [PubMed] [Cross Ref]
[12] Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates. New York: Academic Press; 1998.
[13] Qiao Z.M., Wang J.Y., Han J.S., Luo F. Dynamic processing of nociception in cortical network in conscious rats: A laser-evoked field potential study. Cell Mol Neurobiol. 2007;28:671–687. doi: 10.1007/s10571-007-9216-3. [PubMed] [Cross Ref]
[14] Kisley M.A., Gerstein G.L. Trial-to-trial variability and state-dependent modulation of auditory-evoked responses in cortex. J Neurosci. 1999;19:10451–10460. [PubMed]
[15] Curd-Sneed C.D., Stewart J.J. Oral absorption of sodium pentobarbital and effects on gastrointestinal function. Pharmacol Toxicol. 1989;64:23–27. doi: 10.1111/j.1600-0773.1989.tb00594.x. [PubMed] [Cross Ref]
[16] Powell D.W., Hirschowitz B.I. Sodium pentobarbital depression of histamine- or insulin-stimulated gastric secretion. Am J Physiol. 1967;212:1001–1006. [PubMed]
[17] Cauller L.J., Kulics A.T. A comparison of awake and sleeping cortical states by analysis of the somatosensory-evoked response of postcentral area 1 in rhesus monkey. Exp Brain Res. 1988;72:584–592. doi: 10.1007/BF00250603. [PubMed] [Cross Ref]
[18] Peterson N.N., Schroeder C.E., Arezzo J.C. Neural generators of early cortical somatosensory evoked potentials in the awake monkey. Electroencephalogr Clin Neurophysiol. 1995;96:248–260. doi: 10.1016/0168-5597(95)00006-E. [PubMed] [Cross Ref]
[19] Majewska M.D. Neurosteroids: endogenous bimodal modulators of the GABAA receptor. Mechanism of action and physiological significance. Prog Neurobiol. 1992;38:379–395. [PubMed]
[20] Alitto H.J., Usrey W.M. Corticothalamic feedback and sensory processing. Curr Opin Neurobiol. 2003;13:440–445. doi: 10.1016/S0959-4388(03)00096-5. [PubMed] [Cross Ref]
[21] Ran I., Mathers D.A., Puil E. Pentobarbital induces thalamic oscillations in brain slices, modulated by GABA and glycine receptors. Neuropharmacology. 2004;47:985–993. doi: 10.1016/j.neuropharm.2004.08.001. [PubMed] [Cross Ref]
[22] Montagne-Clavel J., Oliveras J.L. Does barbiturate anesthesia modify the neuronal properties of the somatosensory thalamus? A single-unit study related to nociception in the awake-pentobarbital- treated rat. Neurosci Lett. 1995;196:68–72. doi: 10.1016/0304-3940(95)11847-P. [PubMed] [Cross Ref]

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