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J Clin Invest. 1986 October; 78(4): 914–921.
PMCID: PMC423717

Molecular cloning, expression, and chromosomal localization of the gene encoding a human myeloid membrane antigen (gp150).

Abstract

DNA from a tertiary mouse cell transformant containing amplified human sequences encoding a human myeloid membrane glycoprotein, gp150, was used to construct a bacteriophage lambda library. A single recombinant phage containing 12 kilobases (kb) of human DNA was isolated, and molecular subclones were then used to isolate the complete gp150 gene from a human placental genomic DNA library. The intact gp150 gene, assembled from three recombinant phages, proved to be biologically active when transfected into NIH 3T3 cells. Molecular probes from the gp150 locus annealed with a 4.0-kb polyadenylated RNA transcript derived from human myeloid cell lines and from tertiary mouse cell transformants. The gp150 gene was assigned to human chromosome 15, and was subchromosomally localized to bands q25-26 by in situ hybridization. The chromosomal location of the gp150 gene coincides cytogenetically with the region assigned to the c-fes proto-oncogene, another human gene specifically expressed by myeloid cells.

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Selected References

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  • Griffin JD, Ritz J, Nadler LM, Schlossman SF. Expression of myeloid differentiation antigens on normal and malignant myeloid cells. J Clin Invest. 1981 Oct;68(4):932–941. [PMC free article] [PubMed]
  • McKolanis JR, Finn OJ, Metzgar RS. Characterization of human myelomonocytic antigens using monoclonal antibodies. Prog Clin Biol Res. 1983;133:145–156. [PubMed]
  • Griffin JD, Ritz J, Beveridge RP, Lipton JM, Daley JF, Schlossman SF. Expression of MY7 antigen on myeloid precursor cells. Int J Cell Cloning. 1983 Apr;1(1):33–48. [PubMed]
  • Bernstein ID, Andrews RG, Cohen SF, McMaster BE. Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. J Immunol. 1982 Feb;128(2):876–881. [PubMed]
  • Andrews RG, Torok-Storb B, Bernstein ID. Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies. Blood. 1983 Jul;62(1):124–132. [PubMed]
  • Metcalf D. The granulocyte-macrophage colony-stimulating factors. Science. 1985 Jul 5;229(4708):16–22. [PubMed]
  • Look AT, Peiper SC, Rebentisch MB, Ashmun RA, Roussel MF, Rettenmier CW, Sherr CJ. Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150). J Clin Invest. 1985 Feb;75(2):569–579. [PMC free article] [PubMed]
  • Dana N, Todd RF, 3rd, Pitt J, Springer TA, Arnaout MA. Deficiency of a surface membrane glycoprotein (Mo1) in man. J Clin Invest. 1984 Jan;73(1):153–159. [PMC free article] [PubMed]
  • Anderson DC, Schmalstieg FC, Arnaout MA, Kohl S, Tosi MF, Dana N, Buffone GJ, Hughes BJ, Brinkley BR, Dickey WD, et al. Abnormalities of polymorphonuclear leukocyte function associated with a heritable deficiency of high molecular weight surface glycoproteins (GP138): common relationship to diminished cell adherence. J Clin Invest. 1984 Aug;74(2):536–551. [PMC free article] [PubMed]
  • Sherr CJ, Rettenmier CW, Sacca R, Roussel MF, Look AT, Stanley ER. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1. Cell. 1985 Jul;41(3):665–676. [PubMed]
  • Sariban E, Mitchell T, Kufe D. Expression of the c-fms proto-oncogene during human monocytic differentiation. Nature. 1985 Jul 4;316(6023):64–66. [PubMed]
  • Nienhuis AW, Bunn HF, Turner PH, Gopal TV, Nash WG, O'Brien SJ, Sherr CJ. Expression of the human c-fms proto-oncogene in hematopoietic cells and its deletion in the 5q- syndrome. Cell. 1985 Sep;42(2):421–428. [PubMed]
  • Woolford J, Rothwell V, Rohrschneider L. Characterization of the human c-fms gene product and its expression in cells of the monocyte-macrophage lineage. Mol Cell Biol. 1985 Dec;5(12):3458–3466. [PMC free article] [PubMed]
  • Rettenmier CW, Sacca R, Furman WL, Roussel MF, Holt JT, Nienhuis AW, Stanley ER, Sherr CJ. Expression of the human c-fms proto-oncogene product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines. J Clin Invest. 1986 Jun;77(6):1740–1746. [PMC free article] [PubMed]
  • Look AT, Peiper SC, Douglass EC, Trent JM, Sherr CJ. Amplification of genes encoding human myeloid membrane antigens after DNA-mediated gene transfer. Blood. 1986 Mar;67(3):637–645. [PubMed]
  • Jainchill JL, Aaronson SA, Todaro GJ. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol. 1969 Nov;4(5):549–553. [PMC free article] [PubMed]
  • Lowy DR, Rands E, Scolnick EM. Helper-independent transformation by unintegrated Harvey sarcoma virus DNA. J Virol. 1978 May;26(2):291–298. [PMC free article] [PubMed]
  • Collins SJ, Ruscetti FW, Gallagher RE, Gallo RC. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc Natl Acad Sci U S A. 1978 May;75(5):2458–2462. [PubMed]
  • Koeffler HP, Billing R, Lusis AJ, Sparkes R, Golde DW. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood. 1980 Aug;56(2):265–273. [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Britten RJ, Kohne DE. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Sherr CJ, Fedele LA, Donner L, Turek LP. Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences. J Virol. 1979 Dec;32(3):860–875. [PMC free article] [PubMed]
  • Thomas PS. Hybridization of denatured RNA transferred or dotted nitrocellulose paper. Methods Enzymol. 1983;100:255–266. [PubMed]
  • Donner L, Fedele LA, Garon CF, Anderson SJ, Sherr CJ. McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms). J Virol. 1982 Feb;41(2):489–500. [PMC free article] [PubMed]
  • Graham FL, van der Eb AJ. Transformation of rat cells by DNA of human adenovirus 5. Virology. 1973 Aug;54(2):536–539. [PubMed]
  • Roussel MF, Rettenmier CW, Look AT, Sherr CJ. Cell surface expression of v-fms-coded glycoproteins is required for transformation. Mol Cell Biol. 1984 Oct;4(10):1999–2009. [PMC free article] [PubMed]
  • Lemons RS, Nash WG, O'Brien SJ, Benveniste RE, Sherr CJ. A gene (Bevi) on human chromosome 6 is an integration site for baboon type C DNA provirus in human cells. Cell. 1978 Aug;14(4):995–1005. [PubMed]
  • Kamarck ME, Barbosa JA, Ruddle FH. Somatic cell genetic analysis of HLA-A, B, C and human beta 2-microglobulin expression. Somatic Cell Genet. 1982 May;8(3):385–402. [PubMed]
  • Sherr CJ, Fedele LA, Oskarsson M, Maizel J, Vande Woude G. Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. J Virol. 1980 Apr;34(1):200–212. [PMC free article] [PubMed]
  • Franchini G, Even J, Sherr CJ, Wong-Staal F. onc sequences (v-fes) of Snyder-Theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). Nature. 1981 Mar 12;290(5802):154–157. [PubMed]
  • Le Beau MM, Westbrook CA, Diaz MO, Rowley JD. c-src is consistently conserved in the chromosomal deletion (20q) observed in myeloid disorders. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6692–6696. [PubMed]
  • Shows TB. Genetics of human-mouse somatic cell hybrids: linkage of human genes for isocitrate dehydrogenase and malate dehydrogenase. Biochem Genet. 1972 Dec;7(3):193–204. [PubMed]
  • Goodfellow PN, Jones EA, Van Heyningen V, Solomon E, Bobrow M, Miggiano V, Bodmer WF. The beta2-microglobulin gene is on chromosome 15 and not in the HL-A region. Nature. 1975 Mar 20;254(5497):267–269. [PubMed]
  • Harper ME, Franchini G, Love J, Simon MI, Gallo RC, Wong-Staal F. Chromosomal sublocalization of human c-myb and c-fes cellular onc genes. Nature. 1983 Jul 14;304(5922):169–171. [PubMed]
  • Feldman RA, Gabrilove JL, Tam JP, Moore MA, Hanafusa H. Specific expression of the human cellular fps/fes-encoded protein NCP92 in normal and leukemic myeloid cells. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2379–2383. [PubMed]
  • MacDonald I, Levy J, Pawson T. Expression of the mammalian c-fes protein in hematopoietic cells and identification of a distinct fes-related protein. Mol Cell Biol. 1985 Oct;5(10):2543–2551. [PMC free article] [PubMed]
  • Samarut J, Mathey-Prevot B, Hanafusa H. Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells. Mol Cell Biol. 1985 May;5(5):1067–1072. [PMC free article] [PubMed]
  • Kühn LC, McClelland A, Ruddle FH. Gene transfer, expression, and molecular cloning of the human transferrin receptor gene. Cell. 1984 May;37(1):95–103. [PubMed]
  • McClelland A, Kühn LC, Ruddle FH. The human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence. Cell. 1984 Dec;39(2 Pt 1):267–274. [PubMed]
  • Kavathas P, Sukhatme VP, Herzenberg LA, Parnes JR. Isolation of the gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7688–7692. [PubMed]
  • Sukhatme VP, Sizer KC, Vollmer AC, Hunkapiller T, Parnes JR. The T cell differentiation antigen Leu-2/T8 is homologous to immunoglobulin and T cell receptor variable regions. Cell. 1985 Mar;40(3):591–597. [PubMed]
  • Littman DR, Thomas Y, Maddon PJ, Chess L, Axel R. The isolation and sequence of the gene encoding T8: a molecule defining functional classes of T lymphocytes. Cell. 1985 Feb;40(2):237–246. [PubMed]
  • Maddon PJ, Littman DR, Godfrey M, Maddon DE, Chess L, Axel R. The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell. 1985 Aug;42(1):93–104. [PubMed]
  • Hsu C, Kavathas P, Herzenberg LA. Cell-surface antigens expressed on L-cells transfected with whole DNA from non-expressing and expressing cells. Nature. 1984 Nov 1;312(5989):68–69. [PubMed]

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