PMCCPMCCPMCC

Conseils de recherche
Les critères de recherche 

Avancée

Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
 
From:
doi: 10.1128/MCB.24.12.5369-5382.2004

FIG. 4.

An external file that holds a picture, illustration, etc.
Object name is zmb0120441270004.jpg

Identification of tyrosine residues of ORF5 responsible for binding to cellular proteins. GST and GST-ORF5 mutant fusion proteins purified from either E. coli strain Top10 or TKX1 were mixed with Jurkat T-cell lysates. Polypeptides associated with GST and GST-ORF5 fusion proteins were separated by SDS-PAGE and subjected to immunoblotting with anti-Lck, anti-Fyn, and anti-p85 antibodies. Whole-cell lysate (WCL) obtained from 293T cells transfected with a Flag-tagged SLP-76 expression vector and anti-Flag antibody were used for this assay. WCL was included as a control. GST and GST-ORF5 mutant fusion proteins purified from either E. coli strain Top10 or TKX1 were resolved by SDS-PAGE and subjected to immunoblotting with an antiphosphotyrosine (αpY) antibody and also stained with Coomassie blue (CB).

Images dans cet article

  • FIG. 1.
  • FIG. 2.
  • FIG. 3.
  • FIG. 4.
  • FIG. 5.
  • FIG. 6.
  • FIG. 7.
  • FIG. 8.
Cliquez sur l'image pour l'agrandir.