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Logo of arthrestherBioMed Centralbiomed central web sitesearchsubmit a manuscriptregisterthis articleArthritis Research & Therapy
 
Arthritis Res Ther. 2004; 6(3): R213–R219.
Published online Mar 11, 2004. doi:  10.1186/ar1169
PMCID: PMC416445
Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen
So-Youn Min,1,3 Sue-Yun Hwang,1,3 Kyung-Su Park,2 Jae-sun Lee,1 Kang-Eun Lee,1 Kyung-Wun Kim,1 Young-Ok Jung,2 Hyunk-Jae Koh,2 Ju-Ho Do,2 Haerim Kim,2 and Ho-Youn Kimcorresponding author1,2
1Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Korea
2Center for Rheumatic Disease, Kangnam St. Mary's Hospital, The Catholic University of Korea Medical School, Seoul, Korea
3Co-first authors; order can be switched for bibliographic purposes
corresponding authorCorresponding author.
Ho-Youn Kim: ho/at/catholic.ac.kr
Received October 22, 2003; Revisions requested December 2, 2003; Revised February 10, 2004; Accepted February 26, 2004.
Abstract
Induction of oral tolerance has long been considered a promising approach to the treatment of chronic autoimmune diseases, including rheumatoid arthritis (RA). Oral administration of type II collagen (CII) has been proven to improve signs and symptoms in RA patients without troublesome toxicity. To investigate the mechanism of immune suppression mediated by orally administered antigen, we examined changes in serum IgG subtypes and T-cell proliferative responses to CII, and generation of IL-10-producing CD4+CD25+ T-cell subsets in an animal model of collagen-induced arthritis (CIA). We found that joint inflammation in CIA mice peaked at 5 weeks after primary immunization with CII, which was significantly less in mice tolerized by repeated oral feeding of CII before CIA induction. Mice that had been fed with CII also exhibited increased serum IgG1 and decreased serum IgG2a as compared with nontolerized CIA animals. The T-cell proliferative response to CII was suppressed in lymph nodes of tolerized mice also. Production of IL-10 and of transforming growth factor-β from mononuclear lymphocytes was increased in the tolerized animals, and CD4+ T cells isolated from tolerized mice did not respond with induction of IFN-γ when stimulated in vitro with CII. We also observed greater induction of IL-10-producing CD4+CD25+ subsets among CII-stimulated splenic T cells from tolerized mice. These data suggest that when these IL-10-producing CD4+CD25+ T cells encounter CII antigen in affected joints they become activated to exert an anti-inflammatory effect.
Keywords: collagen-induced arthritis, IL-10, oral tolerance, type II collagen
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