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Infect Immun. 1978 January; 19(1): 107–115.
PMCID: PMC414055

Specificity of salivary-bacterial interactions: role of terminal sialic acid residues in the interaction of salivary glycoproteins with Streptococcus sanguis and Streptococcus mutans.

Abstract

Four highly purified salivary glycoproteins were used to study salivary-bacterial interactions. One pair of glycoproteins was mucin-like in composition, whereas the second pair was not. By an agglutination assay, it was found that only the mucin-glycoproteins agglutinated Streptococcus sanguis and S. mutans. Removal of sialic acid from these molecules resulted in a loss of agglutination of S. sanguis but not of S. mutans. The agglutination phenomenon was shown to require a salivary macromolecule of at least 150,000 daltons.

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Selected References

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  • Arneberg P, Helgeland K, Tjörnhom T. Proline-rich proteins in membranes and contents of monkey (Macaca irus and Cercopithecus aethiops) parotid zymogen granules. Arch Oral Biol. 1976;21(6):379–383. [PubMed]
  • Baig MM, Winzler RJ, Rennert WM. Isolation of mucin from human submaxillary secretions. J Immunol. 1973 Dec;111(6):1826–1833. [PubMed]
  • Cassidy JT, Jourdian GW, Roseman S. The sialic acids. VI. Purification and properties of sialidase from Clostridium perfringens. J Biol Chem. 1965 Sep;240(9):3501–3506. [PubMed]
  • CURBY WA. Device for collection of human parotid saliva. J Lab Clin Med. 1953 Mar;41(3):493–496. [PubMed]
  • Fairbanks G, Steck TL, Wallach DF. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. [PubMed]
  • Gibbons RJ, Houte JV. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. [PubMed]
  • Hay DI, Gibbons RJ, Spinell DM. Characteristics of some high molecular weight constituents with bacterial aggregating activity from whole saliva and dental plaque. Caries Res. 1971;5(2):111–123. [PubMed]
  • Hudson BG, Spiro RG. Fractionation of glycoprotein components of the reduced alkylated renal glomerular basement membrane. J Biol Chem. 1972 Jul 10;247(13):4239–4247. [PubMed]
  • Jacobsen N, Arneberg P. Basic, proline-rich glycoproteins in the submandibular gland secretion of the Cercopithecus aethiops. Comp Biochem Physiol B. 1976;54(3):423–425. [PubMed]
  • Kornfeld R, Kornfeld S. The structure of a phytohemagglutinin receptor site from human erythrocytes. J Biol Chem. 1970 May 25;245(10):2536–2545. [PubMed]
  • Lee YC, Johnson GS, White B, Scocca J. An accelerated system for analysis of neutral sugars in complex carbohydrates. Anal Biochem. 1971 Oct;43(2):640–643. [PubMed]
  • Levine MJ, Ellison SA. Immuno-electrophoretic and chemical analyses of human parotid saliva. Arch Oral Biol. 1973 Jul;18(7):839–853. [PubMed]
  • Levine MJ, Ellison SA, Bahl OP. The isolation from human parotid saliva and partial characterization of the protein core of a major parotid glycoprotein. Arch Oral Biol. 1973 Jul;18(7):827–837. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • MOORE S, STEIN WH. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed]
  • Oemrawsingh I, Roukema PA. Isolation, purification and chemical characterization of mucins from human submandibular glands. Arch Oral Biol. 1974 Aug;19(8):615–626. [PubMed]
  • Payza N, Robert M, Herp A. The molecular weight of bovine and porcine submaxillary mucins. Int J Protein Res. 1970;2(2):109–115. [PubMed]
  • PUSZTAI A, MORGAN WT. Studies in immunochemistry. 20. The action of papain and ficin on blood-group-specific substances. Biochem J. 1961 Dec;81:639–647. [PubMed]
  • ROE JH. The determination of sugar in blood and spinal fluid with anthrone reagent. J Biol Chem. 1955 Jan;212(1):335–343. [PubMed]
  • Sönju T, Christensen TB, Kornstad L, Rölla G. Electron microscopy, carbohydrate analyses and biological activities of the proteins adsorbed in two hours to tooth surfaces in vivo. Caries Res. 1974;8(2):113–122. [PubMed]
  • Sönju T, Rölla G. Chemical analysis of the acquired pellicle formed in two hours on cleaned human teeth in vivo. Rate of formation and amino acid analysis. Caries Res. 1973;7(1):30–38. [PubMed]
  • Sönju T, Rölla G. Distribution of sulphated macromolecules in the salivary secretions of macaca irus. Arch Oral Biol. 1974 Oct;19(10):897–902. [PubMed]
  • SPENCER B. The ultramicro determination of inorganic sulphte. Biochem J. 1960 Jun;75:435–440. [PubMed]
  • Spiro MJ. Subunit heterogeneity of thyroglobulin. J Biol Chem. 1973 Jun 25;248(12):4446–4460. [PubMed]
  • SVENNERHOLM E, SVENNERHOLM L. Quantitative paper partition chromatography of sialic acids. Nature. 1958 Apr 19;181(4616):1154–1155. [PubMed]
  • WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed]
  • Williams RC, Gibbons RJ. Inhibition of bacterial adherence by secretory immunoglobulin A: a mechanism of antigen disposal. Science. 1972 Aug 25;177(4050):697–699. [PubMed]

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