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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Acta Biochim Pol. Author manuscript; available in PMC 2014 March 24.
Published in final edited form as:
Published online 2012 March 17.
PMCID: PMC3963429
NIHMSID: NIHMS564056

Can macular xanthophylls replace cholesterol in formation of the liquid-ordered phase in lipid-bilayer membranes?

Abstract

Lateral organization of membranes made from binary mixtures of dimyristoylphosphatidylcholine (DMPC) or dipalmitoylphosphatidylcholine (DPPC) and macular xanthophylls (lutein or zeaxanthin) was investigated using the saturation-recovery (SR) EPR spin-labeling discrimination by oxygen transport (DOT) method in which the bimolecular collision rate of molecular oxygen with the nitroxide spin label is measured. This work was undertaken to examine whether or not lutein and zeaxanthin, macular xanthophylls that parallel cholesterol in its function as a regulator of both membrane fluidity and hydrophobicity, can parallel other structural functions of cholesterol, including formation of the liquid-ordered phase in membranes. The DOT method permits discrimination of different membrane phases when the collision rates (oxygen transport parameter) differ in these phases. Additionally, membrane phases can be characterized by the oxygen transport parameter in situ without the need for separation, which provides information about the dynamics of each phase. In gel-phase membranes, two coexisting phases were discriminated in the presence of macular xanthophylls—namely, the liquid-ordered-like and so phases. However, in fluid-phase membranes, xanthophylls only induce the solitary liquid-ordered-like phase, while at similar concentrations, cholesterol induces coexisting liquid-ordered and liquid-disordered phases. No significant differences between the effects of lutein and zeaxanthin were found.

Keywords: lutein, zeaxanthin, liquid-ordered phase, spin-labeling, EPR

Introduction

Rohmer et al. (1979) proposed that in prokaryotes dipolar carotenoids act to reinforce and regulate membrane fluidity similarly to cholesterol in eukaryotes. We were intrigued by this hypothesis, and in our earlier papers, we investigated the effects of dipolar carotenoids on the physical properties of lipid-bilayer membranes. We demonstrated that the effects of dipolar carotenoids (including lutein and zeaxanthin) on membrane structure and dynamics are in many ways similar to the effects of cholesterol. Both membrane modifiers increase order, decrease alkyl-chain motion in fluid-phase membranes, and disorder lipids in gel-phase membranes (Subczynski et al., 1992; Subczynski et al., 1993; Kusumi et al., 1986; Subczynski & Kusumi, 1986; Pasenkiewicz-Gierula et al., 1990). Both are known to broaden the gel-to-fluid phase transition and increase the mobility of polar head groups (Subczynski et al., 1989; Wisniewska et al. 2006c). As a rule, the presence of unsaturated alkyl chains moderates the effects of dipolar carotenoids and cholesterol (Subczynski et al., 1989; Kusumi et al., 1986). In saturated membranes, 10 mol% of dipolar carotenoids exerts an effect similar to that of 15–20 mol% of cholesterol (Subczynski et al. 1992; Subczynski et al. 1993; Gruszecki & Strzalka, 2005).

There are major differences between the effects of dipolar carotenoids and cholesterol on membrane organization and dynamics. The ordering effect of cholesterol does not depend on bilayer thickness (Kusumi et al., 1986), whereas the relation between the length of the carotenoid molecule and the thickness of the membrane is a significant factor in determining the effect of dipolar carotenoids on membrane properties (Subczynski et al., 1993; Wisniewska et al. 2006c). Intercalation of cholesterol in lipid-bilayer membranes decreases the frequency of vertical fluctuation of the ends of alkyl chains toward the polar head-group region, but increases the frequency of chain-bending in the membrane center (Yin & Subczynski, 1996; Mainali et al. 2011). Intercalation of dipolar carotenoids in membranes decreases the frequency of alkyl-chain bending at all depths in the lipid bilayer (Yin & Subczynski, 1996).

Numerous investigators have shown that there is a direct correlation between cellular carotenoid content and stabilization of the bacterial membrane (Chamberlain et al., 1991; Rottem & Markowitz, 1979). Moreover, bacteria may also modulate membrane fluidity by changing carotenoid synthesis from dipolar to nonpolar and vice versa (Jagannadham et al., 2000; Chattopadhyay et al., 1997). Bacteria, especially when it lives in extreme conditions, should possess stable membranes that provide a high barrier to nonspecific permeation of small molecules (Jagannadham et al., 2000; Britton, 1995) and that protect the cell against environmental physical stress, such as solar radiation, high and low temperatures, and salt. Incorporation of dipolar carotenoids or glycosylated dipolar carotenoids into the membrane serves this purpose well. Dipolar carotenoids stabilize both halves of the lipid bilayer like transmembrane “rivets,” increasing membrane rigidity by ordering lipid alkyl chains (Subczynski et al., 1992; Subczynski et al., 1993). We would also like to emphasize that polar carotenoids increase the membrane’s hydrophobic barrier to polar molecules and ions (Wisniewska & Subczynski, 1998), which is one of the most fundamental functions of biological membranes. Detailed hydrophobicity profiles may provide the basis to understand membrane permeability for both polar and non-polar molecules (Wisniewska & Subczynski, 1998; Subczynski & Widomska 2009). It should also be noted that monopolar and nonpolar carotenoids (like β-cryptoxanthin and β-carotene) affect membrane properties much less than dipolar carotenoids (Wisniewska et al., 2006c).

In spite of the substantial literature on the subject, many questions about carotenoid-membrane interactions have not been clearly answered or even addressed. These include questions concerning the distribution of carotenoids between membrane domains and the lateral organization of carotenoid-containing membranes. Krinsky and coauthors (Krinsky, 2002; Krynski et al., 2005) observed that the need exists for extensive studies of carotenoid-membrane interactions in model systems to clarify ambiguous results in the literature. We were responsive to these articles: a novel method developed by us, the discrimination by oxygen transport (DOT) method (Subczynski et al., 2007a), was applied to membranes containing carotenoids. We showed that in membranes containing raft domains, the macular xanthophylls, lutein and zeaxanthin, are not distributed uniformly but excluded from saturated raft domains and concentrated in unsaturated bulk lipids (Wisniewska & Subczynski 2006a, Wisniewska & Subczynski 2006b). We believe these results are very significant to understand the role of macular xanthophylls in protecting against lipid peroxidation (Wrona et al., 2004; Pintea et al. 2011 ) in membranes of the eye retina and in preventing age-related macular degeneration (Bone et al., 2001; Landrum et al., 1997).

In this work, we carried out DOT studies on the lateral organization of xanthophyll-containing membranes to examine whether or not lutein and zeaxanthin can parallel other structural functions of cholesterol, including formation of the liquid-ordered (lo) phase. Depending on temperature and cholesterol concentration in phospholipid-cholesterol mixtures, the lo phase can coexist with other phases—namely, solid-ordered (so) and liquid-disordered (ld)—or can form a single lo phase within the entire membrane (Vist & Davis, 1990). One of the simplest model membranes that contains the lo phase is a binary mixture of dimyristoylphosphatidylcholine (DMPC) and cholesterol, for which the phase diagram and lipid motion have been well-characterized (Kusumi et al., 1986; Almeida et al, 1992; Almeida et al, 2005; Recktenwald McConnell, 1981; Shimshick & McConnell 1973). In this work, we simply replaced cholesterol with either lutein or zeaxanthin and carried out DOT measurements for DMPC-xanthophyll and dipalmitoylphosphatidylcholine (DPPC)-xanthophyll membranes below and above the phase-transition temperature. Using the DOT method, we were able to recognize membrane phases induced by the presence of carotenoids. This approach allowed us to study physical characteristics and lipid organization at different depths in the membrane phases as well as measure lipid dynamics. This information was obtained simultaneously for coexisting phases without their separation. In these investigations, we also paid special attention to differences between lutein and zeaxanthin.

Materials and Methods

Materials

Xanthophylls, lutein and zeaxanthin, were purchased from CaroteNature (Lupsingen, Switzerland). DMPC, DPPC, cholesterol, and the spin label 1-palmitoyl-2-(5-doxylstearoyl)phosphatidylcholine (5-PC) (see Fig. 1 for their structure) were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL USA). Other chemicals, of at least reagent grade, were purchased from Sigma-Aldrich (Milwaukee, WI USA).

Fig. 1
Chemical structures of lutein, zeaxanthin together with the structure of DMPC, DPPC, cholesterol and 5-PC spin label.

Preparation of phosphatidylcholine-xanthophyll membranes

The membranes used in this work were multilamellar dispersions of investigated phosphatidylcholine (PC) containing 1 mol% of 5-PC spin label and various amounts of xanthophylls in the range of their miscibility from 0 to 20 mol%. Briefly, these membranes were prepared using the film deposition method (Wisniewska & Subczynski, 1998). Chloroform solutions of lipids, xanthophylls, and spin labels (containing 5 μmol of the total lipid) were combined to obtain a desired compound concentration. Chloroform was evaporated with a stream of nitrogen and with the test tube in constant rotation in order to deposit a uniform film of lipid over the bottom of the tube. The lipid film was thoroughly dried under reduced pressure (about 0.1 mmHg) for 12 h. A buffer solution (10 mM PIPES and 150 mM NaCl, pH 7.0) was added to the dried film at a temperature above the phase-transition temperature of the PC membrane and vortexed vigorously. All preparations and measurements with xanthophylls were performed in darkness or dim light and, if possible, under nitrogen.

EPR measurements

Membranes were centrifuged briefly (16000 g for 15 min at 4°C), and the loose pellet was used for EPR measurements. The sample was placed in a capillary (0.6 mm i.d.) made of the gas-permeable polymer TPX (Subczynski et al., 2005; Hyde & Subczynski, 1989). For measurements of the oxygen transport parameter, the concentration of oxygen in the sample was controlled by equilibration with the same gas that was used for the temperature control (i.e., a controlled mixture of nitrogen and dry air adjusted with flowmeters [Matheson Gas Products, model 7631H-604]) (Subczynski et al., 2005; Hyde & Subczynski, 1989; Kusumi et al., 1982).

The T1s of the spin label were determined by analyzing the saturation recovery (SR) signal of the central line obtained by short-pulse SR EPR at X-band with the use of a loop-gap resonator (Yin et al., 1987; Yin et al., 1990; Subczynski et al., 2005; Yin & Subczynski, 1996). Typically, 105 to 106 decays were acquired with 2048 data points on each decay. Sampling intervals were 2, 4, 8, 16, or 32 ns depending on the sample, temperature, and oxygen tension. The total accumulation time was typically 2 to 5 min. SR signals were fitted by single- or double-exponential functions. When a single-exponential fit was satisfactory, the decay time constant was evaluated with a standard deviation smaller than ±3% from the mean value for independent experiments (for samples prepared independently). When a double-exponential fit was necessary, and satisfactory, the decay times were usually evaluated with standard deviations less than ±5% and ±10% for longer and shorter recovery time constants, respectively.

Outline of theory for the DOT method

In dual-probe SR EPR experiments, molecular oxygen is introduced in the membrane suspension as a relaxation agent. This relaxation agent induces spin exchange, which leads to faster spin-lattice relaxation of the nitroxide. With the DOT method, the observable parameter is the spin-lattice relaxation time, T1, of lipid spin labels, and the measured value is the bimolecular collision rate between molecular oxygen and the nitroxide moiety of spin labels (Ashikawa et al., 1994, Kawasaki et al., 2001). When located in two different membrane environments, the spin label alone most often cannot differentiate between domains, giving very similar (indistinguishable) conventional EPR spectra and similar T1 values. However, even small differences in lipid-packing will affect oxygen partitioning and oxygen diffusion, which can be easily detected by observing the different T1s from spin labels in two locations in the presence of molecular oxygen. SR curves from 5-PC located in membranes made from the PC-xanthophyll mixture were recorded for samples equilibrated with nitrogen and with a specific partial pressure of oxygen (a mixture of air and nitrogen). The double-exponential SR signal indicated the presence of two membrane environments. The results were expressed in terms of the oxygen transport parameter, W, which is proportional to the local oxygen diffusion-concentration product in the specific membrane domain (Kusumi et al., 1982):

equation M1
(1)

Here, the T1s are the spin-lattice relaxation times of the nitroxides in samples equilibrated with atmospheric air and nitrogen, respectively. C(O2) and D(O2) are the local oxygen concentration and the local oxygen diffusion coefficient (see Subczynski et al., 2007 for further explanation).

Results

SR measurements of the oxygen transport parameter

SR measurements for 5-PC in PC-xanthophyll membranes were carried out systematically as a function of xanthophyll concentration, temperature, and the partial pressure of oxygen in the equilibrating gas mixture. All T1 values were determined by fitting the SR signals for 5-PC to single and double exponentials. In deoxygenated samples, all SR signals were fitted successfully to single-exponential functions, indicating that the spin label alone cannot discriminate purported phases (data not shown). All SR signals for membranes equilibrated with air, but not containing xanthophylls, were also fitted successfully to single exponentials, indicating that the DOT method does not discriminate coexisting phases (data not shown). Representative SR signals from samples equilibrated with 40% air for DMPC membranes with 10 mol% lutein are shown in Fig. 2. It is clear that below the phase-transition temperature, at 15°C, the SR signal was fitted successfully to the double-exponential function, indicating the presence of two phases (compare the residual for single- [Fig. 2A] and double-exponential [Fig. 2B] fits). However, above the phase-transition temperature, at 35°C, successful fits were single exponentials (Fig. 2C). Double-exponential fits were satisfactory only for measurements below the phase-transition temperature of DMPC membrane (23.6°C) and DPPC membrane (41.2°C) and for xanthophyll concentrations of 5 and 10 mol%. All SR signals measured above the phase transition and for xanthophyll concentrations greater than 10 mol% were fitted to a single exponential.

Fig. 2
Representative SR signals with fitting curves and the residuals (the experimental signal minus the fitted curve) of 5-PC in the DMPC bilayer containing 10 mol% lutein obtained at 15°C (A and B) and 35°C (C). The liposome suspensions were ...

To assign the two values of the oxygen transport parameter to an appropriate phase, we assumed that the oxygen transport parameter in the lo phase was greater than that in the so phase and smaller than in the ld phase. With this assumption, we accepted the commonly held statement that properties of the lo phase lay between those of gel- and fluid-phase membranes (Loura et al., 2001). We also applied another criterion to assess our results with coexisting phases. The properties of the lo phase that coexist below and above the phase-transition temperature with other phases should change gradually with increased temperature and without any abrupt change at the phase transition. In contrast, the properties of the so phase should change abruptly at the phase transition because this phase disappears at higher temperatures and becomes an ld phase. Therefore, we performed careful measurements of temperature dependences of the oxygen transport parameter across the phase transition for DMPC and DPPC membranes containing different lutein and zeaxanthin concentrations.

Oxygen transport parameter at the phase transition

The oxygen transport parameter was measured as a function of temperature for DMPC and DPPC membranes containing 0, 5, 10, and 20 mol% lutein or zeaxanthin in order to detect two purported phases existing below (so and lo) and above (ld and lo) the phase-transition temperature of the membrane. These types of plots also help to decide which data points come from the so, ld, and lo phases (Subczynski et al., 2007b). Data shown in Figs. 3 and and44 indicate that the lipid environment is homogenous in terms of oxygen transport (only single-exponential SR signals were observed) for the pure DMPC and DPPC membranes (so phase below and ld phase above the phase-transition temperature), and in the presence of 5, 10, and 20 mol% xanthophylls above the phase-transition temperature (lo phase). DMPC and DPPC membranes with overall xanthophyll mole fractions of 5 and 10 mol% exhibit two coexisting phases, with different oxygen transport parameters only below the phase-transition temperature. We attribute them to so and lo. However, in the presence of 20 mol% xanthophylls and below the phase-transition temperature (Fig. 3F), only one phase was found, which we attributed to lo. Points assigned to the appropriate phases are indicated in Fig. 3 and and4.4. As shown above, points at which the oxygen transport parameter changes gradually across the phase transition were assigned to the lo phase (below and above the phase-transition temperature); other points were assigned to the so phase below the phase-transition temperature and to the ld phase above the phase-transition temperature. Such phase behavior was expected and is in agreement with the phase diagram proposed in the discussion section. Some of the discrepancies observed for the lo phase at 5 mol% xanthophylls (Fig. 3B) may be a result of the redistribution of xanthophylls between coexisting so and lo phases below the phase-transition temperature (determined by the related phase boundaries) and their concentration only in the sole lo phase at and above the phase-transition temperature.

Fig. 3
The oxygen transport parameter obtained with 5-PC in DMPC (A,B,C) and DPPC (D,E,F) membranes containing 0, 5, 10, and 20 mol% lutein plotted as a function of temperature. The vertical dashed lines at 23.6 and 41.2°C show the main phase-transition ...
Fig. 4
The oxygen transport parameter obtained with 5-PC in DMPC (A,B) and DPPC (C,D) membranes containing 0 and 10 mol% zeaxanthin plotted as a function of temperature. The vertical dashed lines at 23.6 and 41.2°C show the main phase-transition temperatures ...

The oxygen transport parameter in the lo phase, at all xanthophyll concentrations and at temperatures below the phase-transition temperature, is always greater than that in the pure gel-phase PC membrane and appropriate so phase. Above the phase-transition temperature, the oxygen transport parameter at all xanthophyll concentrations is always smaller in the lo phase than in the ld phase in pure DMPC and DPPC membranes. As expected, an abrupt change in the oxygen transport parameter (approximately, a four-times increase) was observed in the absence of xanthophylls at the main phase-transition temperature.

Location of phase boundaries

As a rule, in the presence of xanthophylls, the phase transition of PC membranes broadens and shifts to lower temperatures (Subczynski et al. 1993, Wisniewska et al. 2006, Widomska et al., 2007; Kolev et al., 1986). The shift is as great as ~3°C, and the phase transition disappears at ~10 mol% xanthophyll concentration. Thus, these phase-transition temperatures form the horizontal phase boundary between the gel-phase membrane containing so and lo phases and the fluid-phase membranes containing ld and lo phases. The vertical phase boundary lies close to 10 mol% xanthophyll concentration. Below the phase-transition temperature and for xanthophyll concentrations smaller than 10 mol% xanthophyll, two coexisting phases are observed (except with 10 mol% lutein in DMPC membranes at 20°C). The position of this vertical phase boundary is similar to the position of the corresponding boundary in PC-cholesterol membranes, assuming that the one molecule of xanthophyll exerts a similar effect on membrane organization as two molecules of cholesterol. Only these two phase boundaries can be determined based on the data presented here and in the literature. Also, based on our data, it can be said that the vertical phase boundary is nonexistent at about 10 mol% xanthophyll concentration above the phase-transition temperature. Starting these experiments, we expected that this boundary would separate the coexisting ld and lo phases from the sole lo phase. Because we detected only the lo phase above the phase-transition temperature, we believe the vertical phase boundary does not exist. Above the phase-transition temperature, xanthophyll molecules are uniformly distributed within the PC bilayer, forming a homogeneous phase.

Discussion

Based on our measurements and data from the literature, we have proposed a phase diagram for PC-xanthophyll membranes (Fig. 5) in which we indicate two regions: Region I, with the single lo phase (above and below the phase-transition temperature), and Region II, with coexisting so and lo phases (below the phase-transition temperature). The horizontal boundary between Regions I and II was determined by measurement of the phase-transition temperature. The vertical boundary between Regions I and II was based on measurements of the oxygen transport parameter.

Fig. 5
Generalized phase diagram for the PC-xanthophyll membranes. Tm indicates the phase-transition temperature for the PC membrane. For details see discussion section.

The most unexpected finding was the lack of two coexisting phases in the DMPC and DPPC membranes above the phase-transition temperature. This is a striking difference with PC membranes containing an appropriate amount of cholesterol for which two coexisting phases—namely, the ld and lo phases—were observed above the phase-transition temperature (Subczynski et al., 2007b; Wisniewska et al., 2008; Laxman et al. 2011). However, we see no conflict between the Gibbs’ phase rule and the phase diagram presented in Fig. 5. The phase boundary between the so + lo region and the ld + lo region should have been at a constant temperature and not tilted (as was the case for the DMPC-cholesterol membrane phase diagram (Almeida et al., 1992). Our measurements and data from the literature indicate that this boundary is tilted (xanthophylls decrease the phase-transition temperature). Thus, according to the Gibbs’ phase rule, it can separate regions containing no more than two phases: the so + lo region and the lo phase.

Almeida et al. (1992) showed another phase boundary in Regions I and II for PC-cholesterol membranes at low cholesterol concentrations (about 5 mol%) (i.e., a boundary between the ld phase and the region of the coexisting lo and ld phases, and a boundary between the so phase and the region of the coexisting lo and so phases). Based on the above information and assuming that one molecule of xanthophyll exerts a similar effect on membrane organization as two molecules of cholesterol, we indicated similar boundaries in a very approximate manner for PC-xanthophyll membranes and positioned them at 2.5 mol% xanthophyll concentration (Fig. 5, vertical dotted line). The xanthophyll solubility threshold should depend on many parameters including the method of membrane preparation, lipid type, the length and degree of unsaturation of alkyl chains, the presence of charges on the lipid headgroup, and inter-headgroup hydrogen bonds. However, it is not possible to control these conditions in different experiments and laboratories. To avoid these problems, we refrained from extending the phase diagram in Fig. 5 above 20 mol% xanthophyll concentration.

Acknowledgments

This work was supported by grants EY015526, EB002052, and EB001980 of the National Institutes of Health.

ABBREVIATIONS

DOT
discrimination by oxygen transport
DMPC
dimyristoylphosphatidylcholine
DPPC
dipalmitoylphosphatidylcholine
5-PC
1-palmitoyl-2-(5-doxylstearoyl)phosphatidylcholine

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