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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
ACS Nano. Author manuscript; available in PMC 2014 March 19.
Published in final edited form as:
PMCID: PMC3959800
NIHMSID: NIHMS546250

Comparison of the Mechanism of Toxicity of Zinc Oxide and Cerium Oxide Nanoparticles Based on Dissolution and Oxidative Stress Properties

Abstract

Nanomaterials (NM) exhibit novel physicochemical properties that determine their interaction with biological substrates and processes. Three metal oxides nanoparticles that are currently being produced in high tonnage, TiO2, ZnO and CeO2, were synthesized by flame spray pyrolysis process and compared in a mechanistic study to elucidate the physicochemical characteristics that determine cellular uptake, subcellular localization, and toxic effects based on a test paradigm that was originally developed for oxidative stress and cytotoxicity in RAW 264.7 and BEAS-2B cell lines. ZnO induced toxicity in both cells, leading to the generation of reactive oxygen species (ROS), oxidant injury, excitation of inflammation and cell death. Using ICP-MS and fluorescent-labeled ZnO, it is found that ZnO dissolution could happen in culture medium and endosomes. Non-dissolved ZnO nanoparticles enter caveolae in BEAS-2B, but enter lysosomes in RAW 264.7 cells in which smaller particle remnants dissolve. In contrast, fluorescent-labeled CeO2 nanoparticles were taken up intact into caveolin-1 and LAMP-1 positive endosomal compartments, respectively, in BEAS-2B and RAW 264.7 cells, without inflammation or cytotoxicity. Instead, CeO2 suppressed ROS production and induced cellular resistance to an exogenous source of oxidative stress. Fluorescent-labeled TiO2 was processed by the same uptake pathways as CeO2 but did not elicit any adverse or protective effects. These results demonstrate that metal oxide nanoparticles induce a range of biological responses that vary from cytotoxic to cytoprotective and can only be properly understood by using a tiered test strategy such as we developed for oxidative stress and adapted to study other aspects of nanoparticle toxicity.

Keywords: Nanotoxicology, Nanoparticle, Reactive oxygen species, Oxidative stress, Dissolution, Nano-bio interface

Ensuring the safety of engineered nanomaterials (NM) is of great importance to the nanotechnology industry that is continuing its march towards a $1 trillion enterprise by 2015.1 While it is likely that most engineered NM are safe, the uncertainty about the novel properties of these materials and how that may relate to nanoscale operations at the biological level has generated considerable concern and could impact the implementation and acceptance of this new technology in society.1 Evaluation of NM safety has to consider the interfacial properties of these materials, including their interaction with proteins, DNA, lipids, membranes, organelles, cells, tissues, and biological fluids. While the traditional toxicological approach to chemical testing involves animal studies as the primary means of hazard assessment, this strategy is costly and labor intensive. As a result, only a few hundred of the 50,000 or so industrial chemicals in the US have undergone toxicity testing and therefore leaves us exposed to new toxicological scares on a continuous basis. While admittedly it is impossible to perform risk assessment and management without in vivo toxicological data, it is becoming clear that animal testing may not provide the base test method when confronted with thousands of new chemicals and nanomaterials. Consequently, we have proposed the development of predictive in vitro toxicological screening to rank NM for priority in vivo testing.1 The National Research Council of the US National Academy of Sciences (NAS) also opined that toxicological testing in the 21st-century should undergo a paradigm shift from a predominant observational science performed in whole animals to a target-specific and predictive in vitro science that utilizes mechanisms of injury and toxicological pathways to guide the judicious use of in vivo studies.2,3 This opinion is compatible with the increasing public demand to reduce or even eliminate animal use for toxicological screening purposes. Our long-term goal is to establish predictive screening models that can be used for NM priority testing in vivo.

Biological systems generally are able to integrate multiple pathways of toxicity into a limited number of pathological outcomes, including inflammation, apoptosis, necrosis, fibrosis, hypertrophy, metaplasia and carcinogenesis.1 To date, the best predictions of ambient and mineral dust particle toxicity have been a small particle size and a large reactive surface area that can lead to toxicological injury through the production of reactive oxygen species (ROS) and oxidative stress.1,4-8 While admittedly these “inadvertently” generated nanoparticles differ from engineered NM, it is noteworthy that many of the toxicological studies on the latter class of materials involve ROS production and the induction of oxidative stress.1 Thus, the theme of particle-induced ROS production and oxidative injury has become an established paradigm for NP toxicity.1,9,10 To facilitate the implementation of this screening procedure for NP toxicity, we have formulated the hierarchical oxidative stress model to provide us with an interlinked range of cellular responses to study NM oxidant injury. At the lowest level of oxidative stress (Tier 1), the induction of antioxidant and detoxification enzymes is mediated by the transcription factor, Nrf2, which regulates the expression of the antioxidant response element (ARE) in the promoter of phase 2 genes.11,12 At higher levels of oxidative stress (Tier 2), this protective response transitions to pro-inflammatory responses based on the ability of ROS to induce redox sensitive signaling pathways such as the MAP kinases and NF-κB cascades.12 At the highest level of oxidative stress (Tier 3), a perturbation of inner membrane electron transfer and the open/closed status of the mitochondrial permeability transition pore (PTP) can trigger cellular apoptosis and cytotoxicity, also known as toxic oxidative stress. Utility of this screening platform has allowed us to compare batches of NP as well as the functionalization of a single particle type to discern between potentially hazardous or potentially safe NP.7 We have also shown that the hierarchical stress paradigm can be applied to the study and prediction of the adverse health effects of ambient ultrafines in animal disease models.13,14 For instance, we have demonstrated that the higher oxidant potential of ambient ultrafine particles (aerodynamic diameter <100 nm) is associated with increased vascular inflammation (atherosclerotic plaques) in apoE knockout mice compared to larger (PM 2.5 μm) ambient particles.13 Moreover, the in vivo vascular injury could be mechanistically linked to synergistic oxidative stress responses that are derived from redox-active particle compounds as well as oxidized LDL components; this synergy generates a genomic footprint that mimics the hierarchical oxidative stress paradigm.14 All considered, these data suggest that the use of the hierarchical oxidative stress paradigm for air pollution and mineral dust toxicity is indeed a predictive scientific platform that can be used to assess particle hazard.15

NP injury can also proceed by non-oxidant paradigms. One example is the ability of NP to organize around them a protein corona that depends on particle size, curvature, shape, and surface characteristics such as charge, functionalized groups and free energy.16 As a consequence of this binding, some particles could generate adverse biological outcomes through protein unfolding, fibrillation, thiol crosslinking, and loss of enzymatic activity.17-19 Another paradigm of NP toxicity is the release of toxic ions when the thermodynamic properties of a material (including surface free energy) favor particle dissolution in a suspending medium or biological environment.20 An example is ZnO nanoparticles that could dissolve under aqueous conditions to form hydrated Zn2+ cations.21 This dissolution is increased under acidic conditions as well as the presence of biological components such as amino acids and peptides.21,22 Although it is known that ZnO particles are toxic to mammalian cells in vitro and the human lung in vivo, the mechanism of toxicity is improperly understood, including to what extent sequential nano-bio interfaces (e.g., in the suspending medium or intracellularly) contribute to dissolution and toxicity. Ultrafine ZnO particles are capable of reaching the alveoli and cause pulmonary inflammation and symptomatic responses in the lung through increased TNFα, IL-6, and IL-8 production.23-25 This manifests as metal fume fever in welders.26,27 Although ZnO dissolution can induce cytotoxicity and apoptosis in mammalian cells,28,29 few of these studies have linked this to a mechanistic paradigm such as oxidant injury. Finally, it is important to consider that some NP may generate bio-protective effects against oxidant injury.30 This could be particularly relevant to cerium oxide.30-32

The three metal oxides that were compared in this study are relevant NM types that are being produced in high tonnage and are in widespread use in a number of consumer products. TiO2 is a semiconductor that it is widely used in catalysts and pigments. This material responds to UV exposure with the generation electron-hole pairs that can lead to biological injury through oxygen radical production or electron capture. However, we can not perform UV exposure in this study because of the potential toxicity in tissue culture cells. CeO2 is widely used in catalysts, fuel additives and polishing agents. Interesting data have been presented suggesting that this material can exert antioxidant effects in tissue culture cells.30 ZnO particles are widely used as polymer fillers and UV absorbers. While ZnO dissolution has been shown to play a role in the acute or chronic toxicity of aquatic organisms, very little is understood about its mechanism(s) of toxicity in mammalian cells.

We compared the effects of ZnO and CeO2 NP in macrophage and epithelial cells according to the oxidant injury paradigm. TiO2 was included as a metal oxide that does not cause toxicological injury to mammalian cells under dark conditions.7 Our data show differential toxic effects with ZnO exhibiting major toxicity based on a mechanism of particle dissolution and Zn2+ release that engages the oxidant injury paradigm through differential cellular uptake and processing in macrophages and epithelial cells. By contrast, internalized CeO2 particles exerted a cytoprotective effect due to its antioxidant properties. TiO2 was inert. These data provide further evidence for the utility of the hierarchical oxidative stress paradigm as a screening tool that can be applied to NP that undergo dissolution or that exert antioxidant effects.

Results

Synthesis and physicochemical characterization of metal oxide nanoparticles

We used flame spray pyrolysis (FSP) to synthesize TiO2, CeO2, and ZnO NP.33-37 This process is used by industry and yields NP with highly reproducible primary particle size, crystallinity and lack of microporosity.35-37 These particles were characterized under dry as well as aqueous conditions. TiO2, ZnO and CeO2 are synthesized as single crystalline particles with primary sizes of 11, 13 and 8 nm, respectively, as determined by BET and XRD (Table 1). The primary particle sizes were also confirmed by TEM analysis (Fig. 1). The TEM micrographs demonstrate the particle shapes as well as their tendency to aggregate under dry conditions (Fig. 1). Interestingly, the particle size distribution in water (Table 1) changes considerably upon suspension in complete tissue culture medium, which contains salts, serum proteins and growth factors that could play a role in particle dispersion and dissolution (Table 1).38 Particularly noteworthy is the shrinkage of ZnO aggregate particles size from 413 to 36 nm (Table 1).

Fig. 1
TEM of the NPs used in this study
Table 1
Physical characterization of selected NPs in dry, aqueous and cell culture media

We studied the dissolution of ZnO NP in water and cell-free culture media in order to estimate the role that dissolved Zn ions may contribute to cellular toxicity. Zinc concentration was measured using ICP-MS. By equilibrating excess commercial ZnO nanopowder (Sigma) with these liquids for 4 days at 22°C, we estimated the maximum total dissolved zinc concentrations that could be attained in the cytotoxicity experiments to be 190 μM in BEGM (BEAS-2B) and 225 μM in complete DMEM (RAW264.7). The high solubility of ZnO nanopowder in the cell culture media indicates the formation of aqueous complexes of zinc ions with organic or inorganic constituents of these complex media. Because it is not known whether the solutions had reached thermodynamic equilibrium with respect to aqueous complex formation and because we did not test for the formation of alternative Zn-bearing solid phases such as Zn(OH)2 these measurements may not represent true saturation concentrations. The data place limits on the total amount of dissolved Zn that may be produced by ZnO NP dissolution in cell culture medium.

We also investigated whether the rate of ZnO NP dissolution in the culture media was fast enough for significant quantities of dissolved Zn to be generated when ZnO particles were added to the cell cultures. Tests of the separation procedure at higher ZnO concentrations (Fig. S1) showed it to be effective for removing non-dissolved ZnO NP from BEGM. However, centrifugation for up to 20 minutes could not completely remove excess ZnO from complete DMEM, as the apparent dissolved Zn concentration consistently exceeded the measured maximum concentration measured with larger particles. This is likely a result both of the high viscosity of the DMEM solution and the breakup of aggregates into smaller fragments that settle more slowly during centrifugation. Nevertheless, the high ZnO solubility in this solution and the observed breakup of the initial aggregates indicate that dissolution is likely also rapid on the timescale of the cell exposure experiments. The ready dissolution of ZnO NP in the aqueous tissue culture media can be expected to reach >80% of the maximum total dissolved [Zn] within three hours. Thus, when less than maximum [ZnO] is used we expect that the cell cultures to be mainly exposed to aqueous Zn ions but when particle concentrations are used that exceed ZnO solubility cells will be exposed to non-dissolved NP.

Differential toxic effects of metal oxide NP in RAW 264.7 and BEAS-2B cells

RAW 264.7 was chosen as representative of a phagocytic cell line while BEAS-2B cells represent transformed human bronchial epithelial cells. Both cell lines have been used extensively for NP and ambient UFP toxicity studies, including assessment of cellular uptake routes and study of cellular stress responses.39,40 For instance, while toxic cationic polystyrene (PS) nanospheres enter a LAMP 1-positive acidifying lysosomal compartment in RAW 264.7 cells, BEAS-2B cells endocytose albumin-coated gold particles and cationic PS nanospheres via caveolae.40 Toxicity testing by following propidium iodide (PI) uptake in cells revealed that ZnO but not TiO2 or CeO2 NP could induce cytotoxicity in RAW 264.7 and BEAS-2B cells (Fig. 2A). Differences in their toxicity profile were confirmed by the MTS assay (Fig. S2) that was used for the performance of time- (Fig. S2A) and dose-response kinetics (Fig. S2B).

Fig. 2
Cell death detection by PI staining and the kinetics of ZnO dissolution in solution

In order to determine whether the ZnO particle effect differs from the toxicity of equimolar amounts of ionized Zn2+, RAW 264.7 and BEAS-2B cells were treated with ZnO NP as well as a ZnSO4 solution. Fig. 2B shows that fully dissolved ZnSO4 induced a significant higher rate of cell death than an equivalent amount of Zn in the form of ZnO. This is compatible with the ZnO dissolution data discussed above in which we estimated that the maximum aqueous [Zn] of 190 μM (BEGM) and 225 μM (CDMEM) is significantly lower than what can be attained with the sulfate salt. However, even when ZnSO4 and ZnO are added at concentrations lower than these levels, so that only dissolved Zn2+ is expected to be present, ZnSO4 is still more toxic than ZnO (Fig. 2B). These findings indicate that the mechanism or rate of aqueous Zn introduction into the cellular environment can have a significant effect on the toxicity of the soluble species. Since at higher [ZnO] only partial NP dissolution is possible, the more rapid rise in the rate of cell death that is observed must be attributed to the presence and uptake of solid ZnO NP.

Assessment of ROS generation under abiotic and biotic conditions

We have previously shown that ROS generation and the induction of oxidative stress is a major toxicological paradigm for ambient and engineered NP.7 ROS generation can originate at the particle surface as a result of the material semiconductor or electronic properties as well as the capability of some materials to perturb electronic transfer processes in the cell such as in the mitochondrial inner membrane. Thus, while the toxicity of ambient ultrafine and quartz particles is dependent on particle composition or adsorbed chemicals that generate ROS abiotically, cationic PS nanospheres are electronically inert but can generate ROS through mitochondrial damage.7 We have previously shown that spontaneous ROS production by NP can be assessed under abiotic conditions with a carbon nanotube (CNT)-NADH peroxidase (Npx)-bioelectrode array.7 This nanobiosensor platform exploits the efficiency and specificity of Npx to detect H2O2 production when a particle suspension is added to the electrode.7 Thus, the amplitude of the deflection during recording of the cyclic voltammogram closely reflects the H2O2 concentration in the solution; the change in the [H2O2] can be calculated by the addition of standardized amounts of H2O2 (Fig. S3). Introduction of NP to this bioelectrode array demonstrates weak H2O2 generation by TiO2, which is in accordance with the UV photoactivation requirements of this material to generate ROS (Fig. 3A). In contrast, both CeO2 and ZnO induced sizable H2O2 production, while cationic PS (NH2-PS) showed a weak deflection that is equivalent to background (Fig. 3A).

Fig. 3
Assessment of H2O2 generation under abiotic and biotic conditions

In order to study cellular ROS generation, dichlorofluorescein acetate (DCF) and MitoSOX Red™ were used to assess the magnitude and kinetics, respectively, for H2O2 and O2.- production under biotic conditions.7 The data show that while ZnO induced the production of both radicals in RAW 264.7 cells, CeO2 and TiO2 were weak inducers of DCF fluorescence and incapable of generating O2.-production (Fig. 3B). Similar results were seen for MitoSOX Red™ fluorescence in BEAS-2B cells, while DCF fluorescence was essentially negative (Fig. 3B). Thus, while both ZnO and CeO2 particles are capable of robust spontaneous ROS generation only the former particle type is capable of biological ROS generation. This suggests differences in the mechanism of biological response generation by these particles. We will demonstrate in a later section that CeO2 exerts an antioxidant effect after cellular uptake.

ZnO induces Tier 1 oxidative stress responses

Particle-induced ROS production can lead to a range of biological responses, depending on the relative abundance of ROS production and the type of cellular pathways that are engaged by oxidative stress.1,7,41 According to the hierarchical oxidative stress hypothesis, the first tier of oxidative stress includes activation of the antioxidant response element in the promoters of phase II genes by the transcription factor, Nrf2.41-43 This leads to the expression of cytoprotective enzymes such as HO-1, NAD(P)H:quinone oxidoreductase 1 (NQO1), superoxide dismutase, catalase, etc..7,41-43 Only ZnO could generate robust HO-1 message and protein expression in RAW 264.7 and BEAS-2B cells (Figs. 4A, ,5A,5A, respectively). ZnO could also induce increased Nrf2 and NQO-1 mRNA expression in these cells (Fig. S4). TiO2 and CeO2 were inert.7

Fig. 4
Assessment of the three tiers of oxidative stress in RAW 274.7 cells
Fig. 5
Assessment of the three tiers of oxidative stress in BEAS-2B cells

ZnO induces Tier 2 oxidative stress responses

If the Tier 1 protection fails to restore cellular redox equilibrium, escalation of the level of oxidative stress is capable of activating pro-inflammatory signaling pathways such as the Jun kinase (JNK) and NF-κB cascades.11,12 While ZnO particles could effectively induce JNK activation in RAW 264.7 (Fig. 4B, left panel) and BEAS-2B (Fig. 5B, left panel) cells as determined by phosphopeptide immunoblotting, TiO2 and CeO2 were ineffective. Moreover, JNK activation occurred in parallel with increased TNF-α and IL-8 production in RAW 264.7 and BEAS-2B cells, respectively (Figs. 4B, ,5B,5B, right panels).

ZnO induces Tier 3 oxidative stress responses

Intracellular calcium release is a major oxidative stress response that can lead to mitochondrial perturbation and cell death.7 This increase in the [Ca2+]i originates from intracellular storage sites such as the endoplasmic reticulum.44,45 ZnO exposure induced a significant and sustained [Ca2+]i increase in both cell types as determined by Fluo-4 fluorescence (Figs. 4C, ,5C).5C). Although this rise in [Ca2+]i is countered by mitochondrial sequestration to keep the [Ca2+]i constant, this protection only succeeds if the organellar concentration remains below a safe threshold. When this threshold is exceeded, it is capable of triggering the mitochondrial permeability transition pore (PTP). Large-scale PTP opening leads to a dissipation of the mitochondrial membrane potential (ΔΨm) and the possible release of proapoptotic factors. Assessment of cellular ΔΨm by JC-1 fluorescence showed that ZnO could effectively increase the % cells with a lowered ΔΨm in RAW 264.7 (Fig. 4C) and BEAS-2B cultures (Fig. 5C). CeO2 and TiO2 did not affect cellular Ca2+ or the ΔΨm.

CeO2 induces cellular protection against an exogenous source of ROS

It has previously been suggested that CeO2 exerts cytoprotective and antioxidant effects based on the electronic properties of the material.30-32 In order to test this hypothesis, both cell types were pretreated with CeO2 before subsequent challenge with a pro-oxidative organic DEP extract; this extract contains a high content of redox cycling organic chemicals that are capable of inducing cellular ROS production and toxic oxidative stress.12,46,47 Our results demonstrate that prior CeO2 exposure could interfere in the PI uptake and superoxide production in RAW 264.7 and BEAS-2B cells (Fig. 6). This result was confirmed by using the MTS assay (not shown). No protective effect was seen if the CeO2 NP were added simultaneous with the oxidant stimulus, suggesting the need for prior cellular processing (not shown).

Fig. 6
Protective effect of CeO2 on DEP induced apoptosis

Electron microscopy and confocal studies show selective cellular processing and impact on cellular ultrastructure

In addition to functional mitochondrial effects, a number of NP (e.g. UFP, fullerenes and micellar nanocontainers) lead to structural mitochondrial changes.7,48,49 Electron microscopy (EM) was used to investigate cellular processing of the metal oxide NP. Although TiO2 and CeO2 particles are internalized by membrane-lined vesicles, this occurred without noticeable structural mitochondrial changes (Figs. S5, S6). By contrast, ZnO particles could not be visualized but the treated cells did exhibit nuclear fragmentation, appearance of apoptotic bodies (Ap) and mitochondrial disappearance (Figs. S5, S6C).

In order to follow more precisely particle processing in the cell, it was necessary to generate fluorescent-labeled NP. We have previously demonstrated how this can be accomplished using polystyrene nanospheres.40 Fluorescent labeling of metal oxide NP was achieved by first grafting the particle surface with aminopropyltriethoxysilane,50,51 followed by the addition of the amine-reactive dye fluorescein isothiocyanate (FITC), as illustrated in Fig. S7. Utilizing the fluorescent-labeled metal oxide NP at concentrations of 25 μg/mL, it was possible to demonstrate distinct routes of particle uptake and processing in BEAS-2B and RAW 264.7 cells (Figs. 7, ,8).8). While it could be shown that all metal oxides localize in a caveolin-1 positive compartment in BEAS-2B cells (Fig. 7), no fluorescence overlap was observed with the late endosomal (LAMP-1+) compartment (not shown). The possibility that the FITC staining profile in Fig. 7A could be due to the release of the label from the particle surface was excluded by the performance of dialysis experiments as well as demonstrating that spiking of the culture medium with FITC molecules alone does not result in a cellular staining profile. Please notice that fewer ZnO particles were taken up in the cell and yielded smaller fluorescent spots (Fig. 7). This likely represents partial particle dissolution in the medium and in the cells following uptake. Also notice that ZnO toxicity was associated with clumping of the caveolae in the rounded up BEAS-2B cell (Fig. 7, bottom panel). In contrast to a caveolar uptake route in BEAS-2B cells, the FITC labeled TiO2 and CeO2 nanoparticles associated with the late endosomal (LAMP-1+) compartment in RAW 264.7 cells (Fig. 8). RAW 264.7 cells exposed to FITC labeled ZnO at concentrations exceeding the culture medium solubility limit exhibited lysosomal clumping even though no fluorescent ZnO particles could be visualized in these cells at all (Fig. 8, bottom panel). We infer that the ZnO particle remnants taken up by these cells are sequestered in lysosomes in which their acidification leads to rapid dissolution and loss of the fluorescent label.

Fig. 7
Confocal microscopy to study the subcellular localization of FITC-labeled metal oxide NP in BEAS-2B cells
Fig. 8
Confocal microscopy to study the subcellular localization of FITC-labeled metal oxide NP in RAW 264.7 cells

Use of confocal microscopy to study cellular Zn2+ release by Newport Green DCF

In order to test whether the toxicity of ZnO NP is associated with Zn2+ release in the cell, we performed cellular staining with the Zn2+-specific fluorescent dye, Newport Green DCF.52 This dye is insensitive to Ca2+ and Mg2+.52 Compared to the diffuse and low intensity staining of untreated RAW 264.7 cells (Fig. 9A upper panel), the ZnO treated cells showed a generalized increase in Newport Green fluorescence with prominent staining of the cellular region that is also stained by the labeled LAMP-1 antibody (Fig. 9A, lower panel). This resulted in a composite orange fluorescence profile, suggesting that Zn2+ derived from the particle remnants concentrate in the lysosomal compartment. The fact that already dissolved ZnSO4 could yield the same staining profile (Fig. S8A) is compatible with the data in Fig. 2 that suggests that dissolved Zn2+ from the cell culture medium contributes significantly to the toxicity possibly by uptake and sequestration in lysosomes.

Fig. 9
Confocal microscopy to study the cellular distribution of Zn2+ in RAW 264.7 and BEAS-2B cells

In BEAS-2B cells, the Newport Green staining overlapped with the caveolin-1 stained compartment in ZnO treated cells (Fig. 9B, lower panel). This likely represents intracellular dissolution of partially dissolved NP is taken up from the culture medium. While ZnSO4 resulted in a generalized increase in cellular fluorescence, this was diffuse and not confined to the caveolar compartment (Fig. S8B).

Discussion

This paper shows that three metal oxide NP under study induced different cellular responses depending on particle composition, route of cellular uptake, subcellular localization and engagement of oxidative stress pathways. Thus, while TiO2 was localized in late endosomal and caveolar compartments in RAW 264.7 and BEAS-2B cells, the particles did not elicit cytotoxic effects under dark conditions. In contrast, cerium oxide NP induced a cytoprotective response to cellular challenge with an exogenous stress stimulus, while ZnO induced an injury response characterized by the production of proinflammatory cytokines (Tier 2) and mitochondrial injury (Tier 3). This toxicity was directly related to particle dissolution and release of toxic Zn2+ in the cell culture medium (both cell types) as well as the uptake of the particle remnants by specific endosomal compartments in the cells. Utilizing FITC-labeled ZnO particles, the uptake in BEAS-2B cells occurs in caveolae. Instead, the toxic Zn2+ accumulated in the lysosomal compartment of RAW 264.7 cells, without any evidence of labeled particles in this compartment. While this represents Zn2+ uptake from the culture medium, it is likely that some contribution come from particle remnants that have shed their FITC label. The Zn2+ accumulation in lysosomes and caveolae was associated with organellar clumping, oxidative cell injury, intracellular Ca2+ release, mitochondrial depolarization, cytokine release, and cytotoxicity. By contrast, CeO2 exhibited no cytotoxic effects in spite of similar endosomal processing as ZnO and TiO2. Moreover, CeO2 protected both cell types against a secondary oxidative stress stimulus, suggesting that the electronic properties of this material may lead to antioxidant protective effects.

This is the first comprehensive study looking at understanding the role of ZnO dissolution in the generation of mammalian toxicity and is in good agreement with similar attempts in bacteria and algae.21 NP dissolution adds an additional layer of complexity to the toxicological mechanisms by which NM could induce biological injury and may also be relevant to the effects of silica, ZnS, and CdSe. ZnO dissolution is particularly relevant to pulmonary toxicity and the development of metal fume fever in welders. This occupational hazard is caused by the inhalation of ZnO or MgO in the welding fumes and is characterized by a sudden onset of fever with X-ray negative respiratory inflammation.23 The injury response is characterized by a significant increase in polymorphonuclear cells in the bronchoalveolar lavage fluid along with an increase in TNF-α, IL-1 and IL-8. A small particle size facilitates the rate of dissolution, with additional enhancement by acidic pH or the presence of biological components such as proteins in the dissolving medium. In this regard, we have observed that the ZnO agglomerates shrink from 413 to 36 nm when transferred from water to tissue culture medium (Table 1). This could reflect the effect of serum components on particle dispersion or dissolution. Serum proteins and lipids have been shown to improve NP dispersion38 and may also assist in dissolution as suggested by the data in Fig. 2C. This size reduction of ZnO NP could facilitate cellular uptake and intracellular dissolution, particularly in the size-limited endosomal compartments. This could constitute a new nano-bio interface where acidification in the endosome or the presence of organic acids could speed up the rate of dissolution. This provides an alternative explanation for why no labeled particles could be seen in the lysosomal compartment of RAW 264.7 cells while fluorescent particles could be observed in a less acidic caveolar compartment in BEAS-2B cells. It is important to mention that particle dissolution does have a potential upside in that it decreases the duration of the tissue exposure and could explain why the symptoms of metal fume fever dissipate within 24-48 hours without any structural lung damage.23,26,27

From a physiological perspective, very little free Zn2+ is present in the cytosol of healthy cells. This divalent cation is mostly bound to proteins such as metallothioneins or sequestered in organelles such as lysosomes and mitochondria.53 The Zn content of mammalian cells is tightly controlled by two integral membrane protein families, namely the Zrt/Irt-like proteins (ZIPs) and zinc transporters (ZnTs).54 The ZIPs facilitate zinc uptake to the cytosol, while the ZnTs promote Zn2+ removal from the cytosol, either by extruding the cation from the cell or sequestering it in cytoplasmic vesicles and organelles. In this regard, ZnT 2, 3, and 4 have been shown to co-localize with LAMP-1 in the lysosomal compartment, while ZnT 2 over-expression leads to significant Zn2+ accumulation within mature lysosomes.54 This suggests that lysosomes play an important role in Zn homeostasis in the cell, which could explain the fluorescence overlap of the Newport Green with the LAMP-1 probe in RAW 264.7 cells (Fig. 9A).

Although a number of in vivo and in vitro studies have addressed the toxicity of ZnO, the mechanism of toxicity in mammalian cells remains unclear. Ultrafine ZnO particles are capable of reaching the alveoli and inducing pulmonary inflammation and metal fume fever.23,25 It is also known that ZnO particles are toxic to several cell types under tissue culture conditions.28,29 We further demonstrate that this toxicity includes the production of pro-inflammatory mediators and cell death, which represent Tiers 2 and 3 in the hierarchical oxidative stress model.1 ZnO dissolution raises the intracellular [Zn2+] and is associated with high levels of ROS production. These oxygen radicals could originate at the particle surface as well as from biological substrates such as damaged mitochondria. By contrast, CeO2 do not generate cellular ROS although the material itself is capable of spontaneous H2O2 production (Fig. 3A). The mitochondrial contribution to ZnO-induced ROS production is suggested by the ultrastructural changes in this organelle (Figs. 4, ,5,5, S5, S6), the increase in MitoSOX fluorescence and change in the membrane potential. Several studies in isolated mitochondria have shown that Zn2+ inhibits cellular respiration by interfering in cytochrome bc1 in complex III as well as with α-ketoglutarate dehydrogenase in complex I.52,53,55 It is also possible that Zn2+ may exert extra-mitochondrial effects contributing to ROS generation, including activation of NADPH oxidase via protein kinase C as well as NO production and generation of peroxynitrite (ONOO-) via the induction of nitric oxide synthase activity.52,53 Not only does Zn2+ trigger ROS generation but cellular oxidant injury could promote further Zn2+release, e.g. from metallothioneins.52,53 Zinc could also inhibit key enzymes in the glycolytic pathway, leading to ATP depletion in cells.56,57 Finally, high levels of Zn2+ could contribute directly to mitochondrial PT pore opening and cytochrome c release, resulting in apoptosis/necrosis in target cells.58,59

An unexpected finding in this study was that CeO2 could induce a protective cellular response in spite of the ability of these NP to generate ROS abiotically (Figs. 4, ,5).5). This observation is compatible with the previous demonstration that this material could prevent increased cellular ROS generation in tissue culture cells.30 This was demonstrated by showing that prior incubation of RAW 264.7 and BEAS-2B cells with CeO2 could protect against the cytotoxic effects of a redox cycling organic DEP extract (Fig. 6). This finding may explain the previous demonstration of cytoprotective CeO2 effects.30-32 Intrinsic material properties could contribute to the antioxidants effects, including the mixed valence state of CeO2 (Ce3+ and Ce4+), allowing this material to act as a free radical scavenger.32 Moreover, the electron defects in nano-ceria are relatively resistant to the radical damage, thereby allowing an autoregenerative reaction cycle (Ce3+→Ce4+→Ce3+) that perpetuates the scavenging activity.60 Finally, it has also been demonstrated that CeO2 is capable of mimicking superoxide dismutase (SOD) activity with a catalytic rate constant that exceeds that of the biological enzyme.61

In summary, our data show differential toxic effects of metal oxide NP of roughly the same size. The route of cellular uptake as well as the material characteristics that lead to engagement of oxidative stress pathways determines the toxicological outcome. Although both ZnO and CeO2 particles could induce spontaneous ROS production, the former induced an injurious while the latter induced a protective response. These data confirm the utility of our tiered oxidative stress screening paradigm that can be adapted to test for paradigms of toxicity that do not directly relate to material oxidant activity. This is likely due to the fact that cellular responses such as inflammation, mitochondrial damage, lysosomal damage, [Ca2+]i flux and cytoxicity also act as injury response pathways to insults other than oxidative stress We are currently using and upgrading this system to develop high-throughput screening methods to study NM toxicity.

Conclusion

The physicochemical and structural properties of NM affect their biological and toxicological effects. We demonstrate that dissolution plays an important role in ZnO induced cytotoxicity. ZnO dissociation disrupts cellular zinc homeostasis, leading to lysosomal and mitochondria damage and ultimately cell death. In contrast, the antioxidant properties of CeO2 protect cells from oxidant injury.

Materials and Methods

Chemicals and Nanoparticles

Propidium iodide (PI), fluorescein isothiocynate (FITC), succinic anhydride, and fluorescamine were purchased from Sigma (St. Louis, MO). 4′,6-diamidino-2-phenylindole (DAPI), 5,5′,6,6′-tetrachloro-1,1′,3,3′ tetraethylbenzimidazolylcarbocyanine iodide/chloride (JC-1), 1-amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2′-amino-5′-methylphenoxy) ethane-N,N,N',N'-tetraacetic acid, pentaacetoxy-methyl ester (Fluo-4), Alexa 594-labeled secondary antibody, Dulbecco's Modified Eagle's medium (DMEM), penicillin/streptomycin, and L-glutamine were purchased from Invitrogen (Carlsbad, CA). The MTS assay kit was obtained from Promega (Madison, WI). Fetal calf serum (FCS) was from Atlanta Biologicals, Inc (Lawrenceville, GA). Rat anti-mouse monoclonal to lysosomal membrane glycoprotein 1 (LAMP-1, 1D4B) and mouse anti-human monoclonal (H4A3) were purchased from Abcam (Cambridge, MA). Rabbit anti-caveolin-1 polyclonal antibody was purchased from BD Biosciences Pharmingen (San Jose, CA). De-ionized water was filtered through a 0.45 μm pore polycarbonate syringe filter (Millipore, Billerica, MA). All chemicals were reagent grade and used without further purification or modification.

Synthesis of metal oxide NP by flame spray pyrolysis

The metal oxide NP were synthesized using a flame spray pyrolysis (FSP) reactor.62 A glass syringe supplied 3 ml/min precursor solution into the oxygen-assisted atomizer nozzle. The precursor solution was dispersed into droplets by a 3.0 L/min oxygen co-flow. The atomizer is surrounded by a ring of eighteen premixed (methane+oxygen) supporting flames placed at 6-mm radius from the center of the nozzle. The flow rate of the methane and oxygen supporting flames were 1.50 and 1.50 L/min, respectively. The maximum flame temperature was estimated to be 2200–2600K.35 The particulate product was collected with the aid of a vacuum pump (Marathon electric type AQC) on two parallel 47 mm membrane filters (Cole Parmer, 1 micron PTFE). The particles were recovered from the filter by scraping without damaging the PTFE coating.

The precursor solvent was m-xylene (anhydrous, 99+ %, Aldrich, CAS Number 108-38-3) in all cases. All precursor solutions were prepared in a nitrogen environment to prevent hydrolysis by ambient humidity. The TiO2 precursor was prepared by mixing titanium (IV) isopropoxide (Aldrich, 97%, CAS Number 546-68-9) with xylene to obtain a 0.5 M metal solution.36,37 The ZnO precursor was prepared by mixing zinc naphthenate <8% (Zn by wt) (Aldrich, CAS Number 12001-85-3) with xylene at appropriate ratios to achieve a 0.5 M metal solution.63 The CeO2 precursor Ce (III) 2-ethylhexanoate, 49% in 2-ethylhexanoic acid (Alfa Aesar, Ce 12% CAS Number 56797-01-4) was mixed with xylene to obtain a 0.2 M metal solution.64 Please notice that FSP is performed at such high temperatures that the final product is devoid of and toxic solvents.

Physicochemical characterization

All NPs were characterized for size, size distribution, shape, and charge. NP shape and structure was assessed by transmission electron microscopy (JEOL JEM 2010, JEOL USA, Inc., Peabody, MA). Microfilms were prepared by placing a drop of the respective NP suspension onto a 200-mesh copper TEM grid (Electron Microscopy Sciences, Washington, PA) and drying at room-temperature overnight. To obtain a representative view, a minimum of 5 images were collected for each sample. Particle size distribution (PSD) was assessed in a ZetaPALS (Brookhaven Instruments Corporation, Holtsville, NY) or ZetaSizer Nano (Malvern Instruments, Westborough, MA). The ZetaPALS measures the dynamic light scattering (DLS) in a suspension at 90° angle and is capable of detecting particles in the size range 2 nm -2 μm. D. ZetaSizer Nano measures DLS at 173° (backscattering) in the range is 0.6 – 6 μm. The ZetaPALS or ZetaSizer Nano was also used to measure the electrophoretic mobility of the NP while suspended in solution. Electrophoretic mobility is used as an approximation of particle surface charge is transformed into zeta potential using the Helmholtz-Smoluchowski equation. Size measurements and electrophoretic mobility of NP were performed in a dilute aqueous NP suspension at pH 7.0 as well as in complete cell culture media at pH 7.4.7

Carbon nanotube (CNT)-NADH peroxidase (Npx)-bioelectrode

We used the CNT-Npx bioelectrode to quantify H2O2 generation by metal oxide NP. The apparatus is comprised of a Npx-bioassembly CNT array as the working electrode and an Ag/AgCl reference electrode with a platinum wire as the counter electrode.7 Electrochemical measurements were performed on an Epsilon system (BASi) at 22°C. The bioelectrodes were equilibrated in the reaction buffer (acetate buffer, pH 6) or water, then scanned to obtain initial cyclic voltammograms (CV) of the enzyme assembly prior to sample measurements. Calibration curves were generated using standard H2O2 solutions at low (< 100 nM) and high (100-4000 nM) concentration ranges. Samples of TiO2, CeO2, and ZnO NP were prepared through serial dilution in water to a final concentration of 10 μg/ml. Scans were taken at 1 hr at a scan rate of 100 mV/sec. Between sample analyses, all samples were tightly sealed and stored in the dark at 4°C. Prior to each measurement, the samples were slowly equilibrated to reach room temperature.

Cell culture and co-incubation with NP

All the NP solutions were prepared fresh from stock solutions (10 mg/ml) and sonicated in culture medium for 10 sec before addition to the tissue culture plates. All cell cultures were maintained in 25 cm2 cell culture flasks, in which the cells were passaged at 70-80% confluency every 2-4 days. RAW 264.7 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Carlsbad, CA) containing 10% FCS, 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM L-glutamine (complete medium). BEAS-2B cells were cultured in BEGM (San Diego, CA) in type I rat tail collagen-coated flasks or plates. To assess the cytoprotective effects of CeO2, cells were pre-incubated with the particles for 24 hours at 25 μg/ml, prior to the addition of 25 μg/ml of a well-characterized diesel exhaust particle (DEP) extract for 16 hours 8. This extract has been previously determined to deliver a toxic insult due to the presence of redox cycling organic chemicals.65

MTS cell viability assay

Cellular viability was determined by the MTS assay, which looks at the reduction of (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) to formazan in viable cells.40 Briefly, 25 × 103 cells were plated onto 96 multi-well plates (Costar; Corning, NY). After incubation with the indicated doses of NP for various lengths of time at 37°C, formazan absorbance was measured at 490 nm. The amount of colored product formed was proportional to the number of live cells in culture. Mean absorbance of non-exposed cells served as the reference value for calculating 100% cellular viability.

RNA isolation

Total RNA was extracted from the cells by using the RNeasy Mini Kit (Qiagen Inc, Valencia, CA) according to the manufacturer's recommendations. Contaminating DNA was removed using a DNA-free kit (Ambion Inc, Austin, Tex). Total RNA was spectrophotometrically quantitated. A 1-μg RNA sample was reverse transcribed using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). The cDNA templates were stored at –40°C.

Real-time RT-PCR

PCR was performed with an iQ SYBR Green Supermix (Bio-Rad Laboratories) using an iCycler (Bio-Rad Laboratories) according to the manufacturer's instructions. The primers for mouse β-actin and human β2-microglobulin, mouse and human HO-1, NQO-1, and Nrf2 were designed according to the Primer3 website (http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi). The mouse sequences for the primers are: (1) β-actin forward: AGCCATGTACGTAGCCATCC and reverse: CTCTCAGCTGTGGTGGTGAA; (2) HO-1 forward: CACGCATATACCCGCTACCT and reverse: CCAGAGTGTTCATTCGAGCA; (3) NQO-1 forward: TTCTCTGGCCGATTCAGAGT and reverse: GGCTGCTTGGAGCAAAATAG; (4) Nrf2 forward: CTCGCTGGAAAAAGAAGTGG and reverse: CCGTCCAGGAGTTCAGAGAG. The human sequences for the primers are: (1) β2-microglobulin forward: ACTGAATTCACCCCCACTGA and reverse: CCTCCATGATGCTGCTTACA; (2) HO-1 forward: TCCGATGGGTCCTTACACTC and reverse: TAAGGAAGCCAGCCAAGAA; (3) NQO-1 forward: TTCTCTGGCCGATTCAGAGT and reverse: GGCTGCTTGGAGCAAAATAG; (4) Nrf2 forward: GCGACGGAAAGAGTATGAC and reverse: GTTGGCAGATCCACTGGTTT. The final PCR mixture contained 1 μL cDNA template and 400 nmol/L of the forward and reverse primers in a final volume of 20 μL. Samples were run concurrently with a standard curve prepared from the PCR products. PCR conditions were three 3-min steps of 94°C and 40 cycles of 94°C for 15 sec, 60°C for 30 sec, and 72°C for 30 sec. Melting curve analysis was used to confirm specific replicon formation. Expression levels were determined from cycle thresholds using a standard curve, normalized to human β2-microglobulin or mouse β-actin expression levels and expressed as fold.

Inductively-Coupled Plasma – Mass Spectrometer (ICP-MS) Analysis of Dissolved Zinc Concentrations in Cell Culture Media

All analyses were done on a Perkin-Elmer SCIEX Elan DRCII ICP-MS. Samples were diluted 1:20 (gravimetrically) in 2% ultra high purity HNO3 and spiked with Ga as an internal standard. Ammonium (NH3) was used as a reaction gas. The zinc concentration was determined from the Zn isotope with a mass:charge ratio (m/z) of 66; the values were monitored at m/z 68 and with Ga as internal standard at m/z 69. Selected samples were spiked with a Zn standard for which recoveries were within 90-110% and better. A preliminary study tested the ability of the ICP-MS instrument to accurately detect dissolved Zn in cell culture media. These solutions were found to be higher viscosity than water, affecting the flow rate to the instrument and the total Zn signal. However, this effect was corrected for by using an internal standard. It is presently estimated that this approach yields Zn concentration measurements in the 1 – 500 uM range that is accurate to better than 5%.

Maximum Zinc Ion Concentrations due to ZnO Dissolution in Cell Culture Media

We estimated the equilibrium zinc concentrations in water, BEGM and DMEM by dissolving excess commercial ZnO nanopowder (Sigma <100 nm) in 25 mL volumes of these aqueous media for four days at room temperature with shaking. 1.5 mL aliquots were centrifuged for one hour with cooling, then 0.1 mL of supernatant was added to 0.9 mL of water and the resulting zinc solution measured by ICP-MS as described above.

Tests of the Separation of ZnO Nanoparticles from Cell Culture Media using Centrifugation

We added ZnO nanoparticles to 25 mL of BEGM and DMEM media at a concentration in excess of the maximum dissolved zinc concentrations measured as described above. The solutions were stirred for two hours, and six 1.5 mL aliquots were removed for tests of solid ZnO separation as a function of centrifugation time in a in a Beckman Coulter Allegra X-22R centrifuge with cooling. As shown in Fig S2, centrifugation at 22,000 × g for 5 minutes successfully removes solid phase ZnO from BEGM, but is unable to remove all the excess ZnO in DMEM solutions.

Assessment of the kinetics of ZnO Nanoparticle Dissolution

Stock solutions were made of commercial 100-nm ZnO particles and of ZnO nanoparticles synthesized by Dr. Lutz Mädler (University of Bremen, Germany and CNSI, USA) at a concentration of 10 mg/mL. Experiments to measure the ZnO dissolution rate in water, high ionic strength solution, and cell culture media were performed as follows. Glass bottles containing 50 ml of solution (one part of water and three parts of solution samples) were allowed to equilibrate to room temperature overnight (22.2°C) with moderate stirring. Each liquid was sampled once, and then 50 uL of a stock ZnO suspension was added to each. The stock suspension was thoroughly mixed by sonication for one minute initially, then by vortexing for 30 seconds before taking material to spike each sample. The ZnO addition procedure took approximately 2 minutes to complete from the first addition to the water sample. Each liquid was sampled by extracting 1.5 mL into a plastic vial, then all four samples were centrifuged for 5 ½ minutes at 20,000 × g in an Eppendorf tube. The vials were carefully removed and 1 mL of supernatant was transferred to a fresh vial for ICP-MS analysis. No visible sediment was observed at any stage.

Cellular staining with fluorescent probes and flow cytometry

Fluorescent probes were diluted in DMEM before the addition to cells for 15-30 min at 37 °C in the dark: (i) 47.5 μg/ml propidium iodide (PI) in 200 μl DMEM (assessment of cell death); (ii) 5 μM JC-1 (assessment of ΔΨm); (iii) 5 μM Fluo-3 (assessment of cytoplasmic free calcium); and (iv) 5 μM Newport Green DCF (assessment of cellular zinc). Flow cytometry was performed using a FACScan or LSR (Becton Dickinson, Mountain View, CA) equipped with a single 488 nm argon laser. Fluo-3 and Newport Green DCF fluorescence was analyzed in the FL-1 channel, while PI was analyzed in FL-2; JC-1 was analyzed in both FL-1 and FL-2. Forward and side scatter were used to gate out cellular fragments.46,66

Determination of TNF-α and IL-8 levels

TNF-α and IL-8 levels after particle treatments were measured by ELISA, as previously described.39,65

Transmission electron microscopy of cells

Harvested cells were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer (PBS) and washed. After post-fixation in 1% OsO4 in PBS for 1 hr, the cells were dehydrated in a graded series of ethanol, treated with propylene oxide and embedded in Epon. Approximated 60-70 nm thick sections were cut on a Reichert-Jung Ultracut E ultramicrotome and transferred to formvar-coated copper grids. The sections were stained with uranyl acetate and Reynolds lead citrate and examined on a JEOL 100CX electron microscope at 80 kV in UCLA BRI Electron Microscopy Core.

Fluorescent labeling of metal oxides

The metal oxide particle surfaces were functionalized by the addition of primary amines, using aminopropyltriethoxysilane (APTS) to attach alkoxy silane groups to the surface hydroxiles. These amines are available for the attachment of fluorescein isothiocyanate (FITC) molecules. Four mg of the metal-oxide nanoparticles were dispersed in 3 mL anhydrous dimethylformamide (DMF). A solution of 0.5 μL APTS diluted in 25 μL DMF was added to the particle suspensions, sonicated and stirred under nitrogen at room temperature for 20 hours. The modified NP were collected by centrifuging and removing the supernatant. After washing, the modified NP were resuspended in 0.5 mL DMF and mixed with a solution of 1 mg FITC and 0.5 mL DMF. The suspension was stirred for 4 hours and the FITC-labeled NP were collected by centrifugation. After thoroughly washing of the labeled materials with DMF, the particles were dried under vacuum to remove the organic solvent and stored as dry powders.

Immunocytochemistry and confocal microscopy

Cultured cells on chamber slides were fixed, permeabilized, and labeled with a standard immunocytochemistry protocol.40 LAMP-1 staining was performed by using a 1:500 dilution of a rat anti-mouse (1D4B) or mouse anti-human monoclonal antibody (H4A3), followed by an Alexa 594-labeled secondary antibody. Caveolin-1 was labeled with a primary rabbit anti-caveolin polyclonal antibody for 1 hr at room temperature, followed by the addition of a 1:500 dilution of secondary Alexa-594-conjugated goat anti-rabbit IgG for 1 hr at room temperature. Slides were counterstained with DAPI in PBS and visualized under a confocal microscope (Leica Confocal 1P/FCS). Confocal images were obtained with a 63× objective. Optical sections were averaged 2-4 times to reduce noise. Images were processed using Leica Confocal Software. Co-localization of labeled nanoparticles with LAMP-1 and caveolin-1 was quantified by the Image J from the NIH.40

Supplementary Material

Acknowledgments

Funding for this study was provided by NSF-funded University of California Center for the Environmental Impact of Nanotechnology (UC CEIN), US Public Health Service Grants U19 AI070453, RO1 ES10553, and RO1 ES015498, as well as the U.S. EPA STAR award (RD-83241301) to the Southern California Particle Center. This work is also supported by the UC Lead Campus for Nanotoxicology Training and Research, funded by UC TSR&TP. L.M. would like to thank the Deutsche Forschungsgemeinschaft DFG (German Research Foundation, Forschungsstipendium MA3333/1-1) for support and the department of Chemical and Biomolecular Engineering in University of California, Los Angeles, CA 90095 for hosting. Funding to JIY was provided by AFSOR (F49620-03-1-0365) and NIH (GM066466). Funding for JIZ was provided by NSF grant CHE0507929. Fluorescent microscopy was performed at the CNSI Advanced Light Microscopy/Spectroscopy Shared Facility at UCLA. BG acknowledges funding from DOE BES DE-AC02-05CH11231 and thanks Joern Larsen for the ICP-MS analyses performed in the Carl Steefel laboratory at LBNL. This work has not been subjected to the EPA for peer and policy review.

Footnotes

Supporting Information Available: Separation of non-dissolved ZnO nanoparticles, calibration of CNT-NADH peroxidase (Npx)-bioelectrode, relative mRNA levels in cells, TEM of cells, surface modification and fluorescent labeling of metal oxides, Confocal microscopy to study the cellular distribution of Zn2+. This material is available free of charge via the Internet at http://pubs.acs.org.

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