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To examine the association between pesticide use during pregnancy and gestational diabetes mellitus (GDM) among wives of licensed pesticide applicators.
Using data from the Agricultural Health Study (AHS), we estimated the association between self-reported pesticide-related activities during the first trimester of the most recent pregnancy and GDM among 11,273 women whose pregnancy occurred within 25 years of enrollment.
A total of 506 (4.5%) women reported having had GDM. Women who reported agricultural pesticide exposure (mixing or applying pesticides to crops or repairing pesticide application equipment) during pregnancy were more likely to report GDM (odds ratio [OR] 2.2 [95% CI 1.5–3.3]). We saw no association between residential pesticide exposure (applying pesticides in the home and garden during pregnancy) and GDM (1.0 [0.8–1.3]). Among women who reported agricultural exposure during pregnancy, risk of GDM was associated with ever-use of four herbicides (2,4,5-T; 2,4,5-TP; atrazine; or butylate) and three insecticides (diazinon, phorate, or carbofuran).
These findings suggest that activities involving exposure to agricultural pesticides during the first trimester of pregnancy may increase the risk of GDM.
Gestational diabetes mellitus (GDM) is a common complication of pregnancy, affecting ~4% of pregnancies in the U.S. (1). Occurrence in the U.S. and worldwide differs by ethnicity, ranging between 1 and 14%, reflecting population-specific patterns seen for type 2 diabetes (2). GDM is regarded as an early stage in the progression to type 2 diabetes and has similar risk factors (3). Known risk factors for GDM include obesity, older maternal age, higher parity, having had GDM or a macrosomic infant in a previous pregnancy, and family history of diabetes (4,5).
Exposure to pesticides may result in abnormal glucose metabolism, increasing the risk of diabetes. In a cohort study of Australian outdoor workers, mortality from diabetes was elevated among those with high pesticide exposures compared with the general population (6). Studies of dioxin exposure (a contaminant of pesticides) are also suggestive of increased risk for type 2 diabetes, hyperglycemia, and hyperinsulinemia (7-9). In addition, there have been several case reports of glycosuria and transient hyperglycemia associated with herbicide or insecticide poisoning (10-12). Furthermore, glucose metabolism disturbances have been observed with exposure to both organo-chlorine and organophosphate insecticides in animal and in vitro studies (13-15). These disturbances include an increase in insulin and blood glucose concentrations, as well as changes in the activity of glucose metabolism enzymes (16-18).
While studies have examined the relationship between pesticides and diabetes (16-18), none have focused on GDM. In the diabetogenic state of pregnancy, women may be particularly susceptible to environmental triggers affecting glucose metabolism. In this article we investigated the risk of developing GDM in relation to pesticide exposures among wives of farmers enrolled in the Agricultural Health Study.
The Agricultural Health Study (AHS) is a large study of licensed pesticide applicators and their families in Iowa and North Carolina. Farmers and commercial applicators using restricted-use pesticides must be licensed every 3 years. Between 1993 and 1997, 52,395 of those applying for a Private Pesticide License in Iowa and North Carolina enrolled in the AHS by completing a brief questionnaire. Seventy-five percent (32,171) of spouses also enrolled in the study by completing a different questionnaire (89.5% responded by mail and 10.5% by telephone). Of these, 61% (19,587) returned a Female and Family Health (FFH) questionnaire, with 18,335 reporting at least one pregnancy. The study was approved by the institutional review boards of the National Institutes of Health, the University of Iowa, and the Battelle Centers for Public Health Research and Evaluation. Additional details of the study are provided elsewhere (19).
Data for this study were obtained at the time of enrollment from both the applicators’ and spouses’ questionnaires (available at http://www.aghealth.org). The FFH questionnaire provided information on reproductive health, including pregnancies that occurred before enrollment in AHS. Detailed information on pregnancy complications and exposure history was collected on the most recent pregnancy. We excluded women whose pregnancy had occurred >25 years before enrollment in the study (n = 5,272), women whose age at the most recent pregnancy was missing (n =677), and women recorded as aged <16 or >49 years (n = 17) at the time of the pregnancy. We also excluded women whose pregnancy ended in a miscarriage, induced abortion, molar, or ectopic pregnancy (n =724) or if the outcome was not reported and the pregnancy did not reach 37 weeks of gestation (n = 104). Further exclusions included women reporting diabetes diagnosed before the age of 20 years (n = 46) and those with missing data on either GDM, pesticide-related activities during pregnancy, or other covariates of interest (n = 222). After these exclusions, 11,273 pregnancies remained for analysis.
Information on pregnancy complications was only obtained for the most recent pregnancy. We categorized women as having had GDM if they answered “yes” to the question, “Did you have gestational diabetes (diabetes just during pregnancy) during this pregnancy?”
To examine pesticide use, we used self-reported information from the FFH questionnaire about pesticide-related activities during the first trimester of the most recent (index) pregnancy. Exposures during the second and third trimesters were not ascertained. We defined four ordered pesticide exposure categories by combining activities with similar potential for pesticide exposure. The resulting categories, from lowest to highest, were: 1) no exposure, 2) indirect exposure (planting, pruning, weeding, picking, or harvesting), 3) residential exposure (applying pesticides to garden or inside house), and 4) agricultural exposure (mixing, applying pesticides to crops, or repairing pesticide application equipment). Women who reported activities pertaining to more than one category were classified according to the category reflecting the highest exposure potential.
Data on specific pesticide use during pregnancy were not collected. To explore the possible role of specific pesticides, we examined the association between GDM and the women’s self-reported ever-use of 50 individual pesticides. Our sample for these analyses was restricted to women who were classified in the agricultural exposure category during the index pregnancy. We report only those pesticides of a priori interest (2,4,5 T; 2,4,5 TP/silvex; and malathion), based on the existing studies (7-9) and those with at least five exposed cases.
We used unconditional logistic regression models to estimate the odds ratios (ORs) of GDM as a function of pesticide exposure. We adjusted for BMI at enrollment (prepregnancy BMI was not available), mother’s age at delivery, parity at the beginning of the pregnancy (previous live births and stillbirths), and race. These covariates were decided a priori based on their being known predictors of GDM. We also adjusted for study center (Iowa and North Carolina). Additionally, for the models including women’s ever-use of pesticides, where appropriate, we adjusted for the five most common pesticides reported by women in the AHS (glyphosate; carbaryl; malathion; 2,4-D; and diazinon) (20). For our analyses, we used the AHS Phase 1 Release P1REL0310.01 data. All statistical analyses were done using SAS version 9.1 (21).
A total of 506 of 11,273 women (4.5%) reported having GDM in their most recent pregnancy. Of the study participants, >97% were white and >50% had greater than high school education (Table 1). Women aged >30 years at the time of the index pregnancy were more likely to report GDM. Women with GDM were more likely to report a BMI >25.0 kg/m2 at the time of enrollment (47%) compared with women without GDM (40%). There were no differences in parity or smoking status between women with and without GDM. Over half the women reported that they had mixed or applied pesticides at some time in their life (57%), and the proportion was similar for those with and without GDM. GDM was more common among women from North Carolina than among women from Iowa. The mean interval between enrollment and the most recent (index) pregnancy was 11.7 years.
Women whose activities during the first trimester of pregnancy involved agricultural exposures (mixing or applying pesticides or repairing pesticide-related equipment) had a twofold increased risk of developing GDM (adjusted OR 2.2 [95% CI 1.5–3.3]) (P <0.0001) (Fig. 1). We saw no increased risk of GDM among women with residential exposures (applying to home or garden) (1.0 [0.8–1.3]) or with indirect exposures (planting, pruning, weeding, picking, or harvesting) (0.9 [0.7–1.1]) during the first trimester of pregnancy (Fig. 1). Women who reported having mixed or applied pesticides at any time before enrollment in the AHS were not at increased risk of GDM compared with those who did not (data not shown).
To reduce the potential for inaccurate recall, we restricted the analysis to women whose pregnancies occurred within 12 years of enrollment (n = 6,004). The adjusted OR for the association between agricultural exposure and GDM was 2.0 (95% CI 1.3–3.4). In addition, when we stratified the analysis by state, results were also unchanged (data not shown).
Pesticide-specific ORs for ever-use of 15 pesticides among women categorized with agricultural exposures during the first trimester of pregnancy (n = 337) are shown in Fig. 2. Risk of GDM was significantly associated with reporting of ever-use of the herbicides 2,4,5-T; 2,4,5-TP; atrazine; and butylate and the insecticides diazinon, phorate, and carbofuran.
Women enrolled in the AHS who reported activities involving agricultural pesticide exposures during the first trimester of their most recent pregnancy had a twofold increased risk of developing GDM. GDM risk was not increased among women who reported using pesticides only in the home and garden or who reported working in the fields. Prior analysis has shown that women in the AHS are involved in many aspects of farm work (22). Only mixing and applying pesticides during the first trimester of pregnancy was associated with GDM. Thus, the association we observed is unlikely to be due to some uncontrolled correlate of participation in general farm activities. We had no information on specific pesticide use during pregnancy. For completeness, however, we examined the association between having ever mixed or applied individual pesticides at some time before enrollment and GDM only among women who reported agricultural exposures during the index pregnancy. These analyses are, however, limited by the small sample sizes. An elevated risk of GDM was associated with ever-use of four herbicides, two organophosphate insecticides, and one carbamate insecticide. Since we do not have information on the timing of exposure relative to pregnancy, any resulting misclassification is likely to have biased these estimates toward null values.
Although our findings of an association between 2,4,5-T and 2,4,5-TP and GDM are based on small numbers, they are of particular interest given the potential for contamination with dioxin in these pesticides (23). Several biologic mechanisms have been proposed for the effects of dioxin-like compounds on glucose metabolism (24). In general, most effects are thought to be mediated through interactions between aryl hydrocarbon receptors and peroxisome proliferator-activated receptor-γ–mediated signaling pathways (24), resulting in an increase in insulin resistance (14) and reduction in glucose transporter activity (15). Epidemiologic studies have also indicated an association between dioxin-like compounds and glucose metabolism (7-9,25). However, because these studies measured serum levels of TCDD (2,3,7,8 tetrachlorodibenzo-p-dioxin) after disease onset, it is possible that the disease process influenced TCDD metabolism. An advantage of questionnaire data in this instance is that our measure of pesticide exposure is unlikely to be influenced by disease processes.
Laboratory data have suggested a possible effect of malathion (an organophosphate) on glucose metabolism, including increases in insulin and blood glucose concentration and changes in key enzymes involved in gluconeogenesis, glycogenolysis, and glycolysis. Similar mechanisms may be involved for other organophosphates (16,17). In our study we did not find an increased risk of GDM among women who reported agricultural exposures during pregnancy and ever-use of malathion (Fig. 2). Malathion is widely used for home and garden as well as for crop applications, and its purchase does not require a pesticide license (20). It is possible that we missed an association with malathion by focusing solely on women who reported mixing or applying pesticides to crops during the first trimester of the pregnancy. However, when we examined women reporting home and garden use of pesticides during the first trimester we still failed to see an association.
We saw an increased risk of GDM associated with two other organophosphate insecticides (diazinon and phorate) and one carbamate insecticide (carbofuran). Interestingly, there have been several case reports of glycosuria with and without hyperglycemia following pesticide poisoning with organophosphate insecticides and carbamates (10-12).
This study provides data on a large number of women who reported performing specific tasks during the first trimester of their most recent pregnancy. Even though GDM in this study was self-reported, the reported frequency of 4.5% is in the expected range for the U.S. (3–5%) (4). Although our analysis excluded women with diabetes diagnosed aged <20 years, we were unable to exclude preexisting type 2 diabetes for all women, since age at diagnosis of type 2 diabetes was asked in broad categories. Our GDM question asked specifically about “diabetes only during pregnancy” to facilitate more accurate reporting. Even so, bias from self-reported GDM may be a concern. Several studies in diverse populations have shown that maternal recall of rare obstetric complications (including GDM) is relatively accurate when compared with medical records (26-28). These studies report high sensitivity, which suggests that there are not likely to be unreported cases among the control subjects. While some women who report GDM may not have it, this would tend to bias our results toward the null.
The data on reported pesticide exposure during pregnancy span 25 years, which may affect accuracy of exposure reporting. However, we have previously found that farmers and their families tend to reliably report pesticide exposure history (29-31). Furthermore, our estimates were unchanged when we restricted the analysis to pregnancies that occurred within 12 years of enrollment. Unfortunately, the sample size was too small to focus on a shorter interval.
Our estimates may be affected by reporting bias if women who had GDM tended to over-report agricultural pesticide use during pregnancy or if women who had been exposed were more likely to report GDM. This is unlikely since GDM was not the primary focus of the questionnaire, and we characterized the exposure by aggregating responses to several individual questions. Furthermore, the increased risk observed in our estimates was associated only with activities with a greater potential for exposure to farm pesticides, while we saw no increase in risk with other activities.
Although adjusting for BMI had little effect on risk estimates, our ability to control for it was limited by the fact that we relied on weight reported at enrollment rather than before the index pregnancy. Even though BMI tends to track over time, BMI at enrollment will be a better surrogate for recent pregnancies than for those more distant in time. It was, however, reassuring that restricting the analysis to more recent pregnancies did not change the results. Furthermore, although BMI was not a confounder in our analysis, it was significantly associated with GDM. Due to small sample size, we were unable to explore whether race modified the association between agricultural exposure and GDM.
Pregnancy is a known diabetogenic state resulting from decreased insulin sensitivity (32,33). The inability to compensate for the decreased insulin sensitivity results in hyperglycemia above the normal pregnancy ranges. Although much is known about common risk factors for GDM, our understanding of whether and how environmental exposures may affect risk is still limited. Research shows that 20–50% of women with GDM will develop type 2 diabetes within 5–10 years (34). Thus, understanding any potential effect of environmental exposures on glucose tolerance during pregnancy may have substantial public health importance beyond the direct effects on GDM.
This research was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Environmental Health Sciences, and National Cancer Institute.
The authors thank Stuart Long (Westat), the staff from the Iowa and North Carolina Field Stations, and the pesticide applicators and their families for their work and participation in Agricultural Health Study.