High fat/cholesterol-induced expression of BACE1. A. Total RNA was isolated from the corteces of C57BL/6 and LDLR−/− mice fed the two diet regimen and analysed by qPCR for BACE1 mRNA. * p < 0.05 versus the normally fed control group. # represents significant (p < 0.05) difference of normally fed LDLR−/− group from normally fed C57BL/6 (control) group. B. Corresponding tissue extracts were analysed by immunoblot for BACE1 protein levels using BACE1-specific antibodies. The line graphs below show the relative BACE1 protein levels.