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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Recept Signal Transduct Res. Author manuscript; available in PMC 2013 December 23.
Published in final edited form as:
PMCID: PMC3870196
NIHMSID: NIHMS533442

Ric-8 regulation of heterotrimeric G proteins

Abstract

Resistance to inhibitors of cholinesterase 8 proteins (Ric-8A and Ric-8B) collectively bind the four classes of heterotrimeric G protein α subunits. Ric-8A and Ric-8B act as non-receptor guanine nucleotide exchange factors (GEFs) toward the Gα subunits that each binds in vitro and seemingly regulate diverse G protein signaling systems in cells. Combined evidence from worm, fly and mammalian systems has shown that Ric-8 proteins are required to maintain proper cellular abundances of G proteins. Ric-8 proteins support G protein levels by serving as molecular chaperones that promote Gα subunit biosynthesis. In this review, the evidence that Ric-8 proteins act as non-receptor GEF activators of G proteins in signal transduction contexts will be weighed against the evidence supporting the molecular chaperoning function of Ric-8 in promoting G protein abundance. I will conclude by suggesting that Ric-8 proteins may act in either capacity in specific contexts. The field awaits additional experimentation to delineate the putative multi-functionality of Ric-8 towards G proteins in cells.

Keywords: Chaperone, G protein α, guanine nucleotide, exchange factor (GEF)

Resistance to inhibitors of cholinesterase 8 (Ric-8) proteins are unique regulators of heterotrimeric G protein α subunits. The RIC-8 gene was discovered in Caenorhabditis elegans from a mutagenesis screen that sought mutations of genes required for neurotransmission (1). Complementation groups in the RIC screen included components of the synaptic vesicle priming process such as the SNARE machinery. Heterotrimeric G protein q signaling components were also identified as RIC mutants and included egl-30 (Gαq), egl-10 (RGS) and unc-13 (encodes a diacylglycerol binding sensor). In subsequent studies, Ric-8 mutants were shown to be epistatic to mutations in genes that encoded three different classes of G protein α subunits. Genetically, Ric-8 was predicted to act upstream of, or parallel to, Gαq and Gαs to provide diacylglycerol and cAMP second messenger regulation of synaptic vesicle priming (24). Ric-8 worked with Gαo to control spindle pole movements that regulated mitotic spindle positioning in C. elegans embryos (5).

The C. elegans genetic studies were suggestive that Ric-8 interacted with G proteins. Demonstration of physical binding was first made with mammalian versions of the proteins. Two mammalian homologs of C. elegans Ric-8 were identified in yeast two-hybrid screens conducted to identify novel interactors of Gαo and Gαs baits (6,7). Our 2003 work demonstrated the enzymological basis of the interaction; Ric-8A is a non-receptor guanine nucleotide exchange factor (GEF) for Gα subunits. Purified Ric-8A enhanced the rates of GTPγS binding to purified Gαi, Gαq and Gα13 subunits, but not Gαs. This work was followed up by demonstration that mammalian Ric-8B and Xenopus Ric-8 acted as GEFs for the Gαs/Gαolf-class (8,9). A key feature of Ric-8-catalyzed Gα subunit in vitro nucleotide exchange stimulatory activity that extends to understanding the physiological function of Ric-8 in cells comes from the Michaelis–Menten enzymatic study, in which the GTP substrate dependence of Ric-8-catalyzed nucleotide exchange was examined. Measurement of Gαs steady state GTP hydrolysis showed that Gαs had an intrinsic Vmax of 0.28 mol GTP hydrolyzed mol Gαs−1 min−1, and an intrinsic GTP binding Km of ~385 nM. The full-length Ric-8B isoform dramatically elevated both these parameters nearly 10- and 100-fold, respectively (Figure 1A). Mechanistically, Ric-8B bound to Gαs-GDP (guanosine diphosphate) and initiated rapid GDP release. Ric-8B stabilized the nucleotide-free Gαs intermediate. The high affinity that Ric-8B has toward nucleotide-free Gαs was reflected by the higher concentrations of GTP (elevated Km) that were required to dissociate the complex to produce free Gαs-GTP and Ric-8B (Figure 1B). The important point that extends to understand the function of Ric-8 in cells is that Ric-8 proteins have highest affinity for open, nucleotide-free conformations of Gα proteins.

Figure 1
Ric-8 proteins stabilize the nucleotide-free form of Gα subunits to stimulate GTP-dependent Gα nucleotide exchange. (A) The GTP substrate dependence of Gαs steady state GTP hydrolytic activity was measured in the presence or absence ...

Since the original finding that Ric-8 proteins act as GEFs towards Gα subunits in vitro, there have been many investigations of the effects of Ric-8 gene perturbation on G protein function in cells. The evidence from studies in model organisms and mammalian cells has shown that Ric-8 homologs collectively influence the signaling properties of all four classes of G protein α subunits. Ancestral Ric-8 and mammalian Ric-8A influence Gαi/o-class regulation of spindle pole movements to position the mitotic spindle during symmetric and asymmetric cell division (1016). Caenorhabditis elegans Ric-8 and mammalian Ric-8A affected Gαq-dependent neurotransmission (2,17). Ric-8B expression enabled reconstitution of Golf-dependent odorant receptor signaling in a heterologous system and positively influenced Gαs/Gαolf signaling and protein stability (1821). Mouse Ric-8A and Ric-8B were required for efficient basal and hormone-stimulated adenylyl cyclase activity in embryonic stem cells (22). Ric-8A was recently purported to act as the GEF that propagated signals derived from the platelet-derived growth factor receptor (PDGFR) to directly activate Gα13 subunits and consequently regulate dorsal ruffling and cell migration (23). In most of these studies, data were also provided to show that Ric-8 proteins positively influenced Gα subunit plasma membrane localization and G protein abundance. We favor the hypothesis that the function of Ric-8 proteins in cells is to promote G protein abundance and that Ric-8 GEF activity per se is not necessarily for the purpose of producing activated Gα-GTP to engage downstream effectors. Most observations of Ric-8 pleotropic regulation of varied G protein signaling pathways can be explained because Ric-8 proteins positively regulate G protein abundance in cells.

In support of this idea, we found that co-expression of recombinant GST-tagged Ric-8A or Ric-8B with recombinant Gα subunits in Sf9 or High-Five™ (invitrogen) insect cells led to a dramatic up-regulation of the levels of expressed Gα subunits by 25-to-50-fold. The elevated abundances of the recombinant Gα subunits and the finding that much of the over-produced G protein was bound to GST-Ric-8A permitted the development of a new purification procedure for Gα subunits. GST-Ric-8:Gα complexes from insect cell lysates were trapped on a glutathione Sepharose matrix. G proteins were eluted from the matrix with equation M1 buffer that dissociates Ric-8 and Gα. The eluted Gα subunits were highly pure, devoid of contaminating Ric-8 protein and produced in an unprecedented yield. Gαq and Gα13 subunits could formerly be produced only in low quantity (24,25). The new technique using GST-Ric-8 allows the production of ~5–20 mg of Gα subunit per liter of insect cell culture (26). This technique has proven useful to the field in terms of the increased availability of purified G protein subunit reagents. From the perspective of Ric-8 biology, this study prompted us to investigate potential roles of Ric-8 proteins in the control of G protein biosynthesis and/or resistance to protein turnover.

To this end, we worked with collaborators at Regeneron Pharmaceuticals to produce Ric-8A or Ric-8B knockout mice. Both strains were embryonic lethal and died early during embryogenesis. Attempts to ascertain the timing and cause of embryonic death were difficult, as no knockout embryos of either Ric-8 strain could be recovered as early as Day E8.5. An independently derived Ric-8A knockout mouse was shown to exhibit embryonic death between Days E6–E8 (27). These Ric-8A null embryos had severe gastrulation defects of unknown etiology. To circumvent the inability to use Ric-8 knockout mice as models, we harvested live blastocysts from Ric-8A+/− or Ric-8B+/− intercrosses and cultured Ric-8A−/− and Ric-8B−/− embryonic stem cell lines from them (22). Ric-8 null mES cells exhibited dramatic G protein signaling defects. Hormone and forskolin-stimulated cAMP production was blunted in both the Ric-8A and Ric-8B-null cell lines. GPCR-dependent activation of Rho GTP signaling was blunted in the Ric-8A knockouts. These signaling defects were attributed to the dramatic reductions in steady state G protein membrane abundances that were observed. Ric-8A−/− cells had ~95% reductions in the levels of Gαi/q/13, and Ric-8B−/− cells had a dramatic reduction in the level of Gαs. This pattern matches the pattern of Ric-8A or Ric-8B nucleotide exchange stimulatory activity towards Gα subsets. Interestingly, the total Gβ level (Gβ1–4) was reduced by ~50% in the Ric-8A−/− cells when compared to wild type or Ric-8B−/− cells. This was attributed to a bulk reduction in the amount of Gα subunits in the Ric-8A null cells, with the Gαi-class contributing the most to the total pool. The extent of the G protein abundance defects in a crude membrane fraction was greater than the overall reductions observed in whole cells, suggesting the probability of a Gα membrane-targeting defect that was manifested by the absence of Ric-8A.

Pulse-chase labeling studies were initiated to investigate the timing and extent of nascent Gα subunit membrane association. G protein subunits are produced on soluble ribosomes and assembled as heterotrimers on the outer leaflet of the endoplasmic reticulum membrane prior to trafficking of the intact heterotrimer to the cell surface (28,29). mES cells were briefly pulse labeled with radiolabeled amino acids and then chased with unlabeled amino acids. During the chase, cells were fractionated into crude membranes and cytosol. Gαi1/2 or Gαq were immunoprecipitated from the fractions and visualized by autoradiography. Virtually all of the nascent Gαq remained soluble in the absence of Ric-8A, whereas most of the Gαq accumulated on a membrane in cells expressing Ric-8A (Figure 2). This defect was observed for Gαi as well, although the extent of the defect was not as great. We reasoned that Gαi is the only class of G protein that becomes lipidated (myristoylation) during translation in the cytosol and this may confer an advantage in membrane association over other Gα subunits that become lipidated only upon achieving membrane association (e.g. Gαq). An extended pulse-chase study was then conducted to measure the rates of G protein turnover in Ric-8A−/− and wild type cells. Gαi, Gαq and Gβ were turned over nearly 10-fold faster in Ric-8A−/− cells. We reasoned that the inability of Gα subunits to attain membrane residence in the absence of Ric-8A function resulted in enhanced G protein degradation. That is, the primary Gα subunit biosynthetic defect imparted by Ric-8A absence was realized as a secondary consequence of enhanced protein turnover of the mis-targeted, cytosolic G proteins.

Figure 2
Nascent Gα q is defective in association with cellular membranes in mES cells lacking Ric-8A. Ric-8A−/− mES cells stably expressing Ric-8A cDNA or pcDNA were pulse labeled with [35S]-methionine and -cysteine for 10 min and chased ...

The sum of these studies concluded that Ric-8A acted upon Gα subunits at an early point during biosynthesis. We proposed that Ric-8A could work with either the TriC/CCT and/or HSC70/90 chaperone systems to promote Gα subunit folding, or could act after folding upon soluble Gα subunits to escort them to the proper endomembrane for G protein heterotrimer assembly (Figure 3). We have begun investigations using cell-free translation systems to address these possibilities and have found preliminarily that Ric-8A is required for Gα subunit folding (30).

Figure 3
Model of Ric-8 action during G protein biosynthesis. G protein α subunits are translated by cytosolic ribosomes. The CCT and/or HSC70/HOP/HSC90 chaperone systems were suggested to participate in the folding nascent Gα subunits (35 ...

Perspective

Most research describing roles for Ric-8 in G protein regulation has intimated that Ric-8 acts akin to the action of a GPCR. In this role, Ric-8 is proposed to control Gα subunit activation status in cells as a GEF, producing Gα-GTP and the consequent activation of traditional and non-traditional G protein effector enzyme systems. Most of the observations that contributed to this theory, however, are explained equally as well by Ric-8 controlling the abundance of the signaling G protein subunits. Going forward, the question of how Ric-8 regulates G protein function is probably not limited to a single answer. Ric-8 may have dual function(s) within the cell towards G proteins as a GEF activator and molecular chaperone.

Independent studies have provided some evidence of Ric-8 features that are not intuitively consistent with Ric-8 acting solely as a chaperone during Gα subunit biosynthesis. In asymmetric cell division, biochemical work demonstrated that Ric-8A can act upon Gαi:GPR/GoLoco-domain protein complexes to dissociate them and produce Gαi-GTP (3032). Caenorhabditis elegans Ric-8 and HeLa cell Ric-8A are recruited to mitotic spindles and spindle pole regions during mitosis (14,16). Studies in mammalian cells have shown recruitment of fluorescent protein tagged Ric-8 proteins to the cell periphery in response to chronic GPCR agonist treatments (6). Ric-8A was found to bind to neural cell adhesion molecule (NCAM) and appeared to co-localize at the cell surface with NCAM (33). Recently Blumer and Lanier et al. provided the first experimental evidence that distinguished two separable functions of Ric-8A in cells. Ric-8A regulation of Gαi abundance was insensitive to pertussis toxin (PTX), whereas Ric-8A regulation of bioluminescence resonance energy transfer (BRET) interactions between Gαi and AGS3 or AGS4 at the plasma membrane were PTX sensitive (34). ADP-ribosylated Gαi is not a substrate for Ric-8A-catalyzed nucleotide exchange (16). The substrate of PTX is the Gi heterotrimer. Therefore, it is logical that Ric-8 action as a chaperone of nascent Gαi that has not yet bound Gβγ is PTX insensitive, and that Ric-8 action on maturated Gαi at the cell surface (in complex with AGS3 or AGS4) is PTX sensitive. Future work will provide insight into the multiple roles that Ric-8 proteins have in cells including mechanistic detail of Ric-8 chaperoning and/or GEF functions.

Acknowledgments

This work is supported by NIH grant GM08824 to G.G.T.

Footnotes

Declaration of interest

The author reports no declaration of interest.

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