Reconstituted GltPh remains active after 3L4 cleavage.A, Na+-driven initial transport rates of l-[3H]Asp into proteoliposomes containing GltPhWT and GltPhXa reconstituted after incubation at 37 °C for 24 h in the presence (+) and absence (−) of Factor X. Data are normalized to untreated GltPhWT data and are the average of three datasets. Error bars indicate S.E. B, nonreducing SDS-polyacrylamide gel of the treated protein reconstituted into the proteoliposomes used in A. Arrows indicate the positions of Factor X (Fxa), full-length protein (FL), and the proteolytic C-terminal (CTF) and N-terminal fragment (NTF).