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J Neurosci. 2010 October 27; 30(43): 14430–14439.
PMCID: PMC3842486

Abl Family Tyrosine Kinases Are Essential for Basement Membrane Integrity and Cortical Lamination in the Cerebellum

Abstract

The Abl family nonreceptor tyrosine kinases, consisting of closely related Abl and Arg (Abl-related gene), play essential roles in mouse neurulation, but their functions in the subsequent development of CNS are poorly understood. Here, we show that conditional deletion of Abl in precursors of neurons and glia on an Arg knock-out background leads to striking cerebellar malformations, including defects in anterior cerebellar morphogenesis, granule cell ectopia, and hypoplasia. Time course analyses reveal that the abnormal anterior cerebellar foliation results from local disruptions of the basement membrane (BM) located between radial glial endfeet and the meninges during embryonic cerebellar development. Granule cell ectopia and hypoplasia are also associated with the breaches in the BM and abnormal Bergmann glial networks during postnatal cerebellar development. In vitro culture experiments indicate that Abl/Arg-deficient granule cells can interact with glial processes and proliferate normally in response to sonic hedgehog compared to cells isolated from control mice. Consistent with these findings, selective ablation of Abl family kinases in cerebellar granule cells alone does not cause any abnormality, suggesting that deletion of Abl/Arg from glia is likely required for the mutant phenotype. Together, these results provide compelling evidence that Abl and Arg play key redundant roles in BM maintenance and cortical lamination in the cerebellum.

Introduction

Cortical structures in the mature mammalian CNS consist of defined cell layers that are formed by a series of well orchestrated cell specification and migration events. The cerebellum has long provided an excellent model system to study the lamination process (Hatten, 1999; Sillitoe and Joyner, 2007). In the cerebellar cortex, Purkinje cells arise from the ventricular zone and migrate radially toward the cerebellar surface. Granule cell precursors (GCPs) are generated by the rhombic lip and move tangentially across the surface of the cerebellar primordium to form a secondary germinal zone, the external granular layer (EGL). After birth, granule cells that are produced in the EGL migrate radially inward through the Purkinje cell layer (PCL) to form the internal granular layer (IGL), and this occurs simultaneously with the formation and growth of the folia.

The pial basement membrane (BM) is a network of extracellular matrix (ECM) proteins secreted by meningeal fibroblasts (Sievers et al., 1994), and its integrity is critical for corticogenesis. Mutant mice with deletions in some members of BM components (perlecan and laminin α5 and γ1), their cellular receptors (α-dystroglycan, integrins β1 and α6), or downstream associates of integrins such as FAK (focal adhesion kinase) and ILK (integrin-linked kinase) show BM disruptions and cortical and cerebellar abnormalities (Georges-Labouesse et al., 1998; Miner et al., 1998; Costell et al., 1999; Graus-Porta et al., 2001; Halfter et al., 2002; Moore et al., 2002; Beggs et al., 2003; Niewmierzycka et al., 2005; Belvindrah et al., 2006, 2007; Mills et al., 2006; Satz et al., 2008). These defects resemble the cobblestone lissencephaly found in a subclass of congenital muscular dystrophy, and most of them are caused by aberrant glycosylation of α-dystroglycan, a laminin receptor (Olson and Walsh, 2002; Barresi and Campbell, 2006).

The Abl family nonreceptor tyrosine kinases contain Src homology 3 (SH3), SH2, tyrosine kinase domains, and unique carboxyl-terminal halves with many functional domains (Pendergast, 2002). A prominent function of Abl kinases is to transduce signals from cell surface receptors into cytoskeletal reorganizations that have dramatic effects on cell morphology and motility (Woodring et al., 2003; Bradley and Koleske, 2009). Mouse embryos deficient in both Abl and Arg suffer from a defect in neurulation and die at embryonic day (E) 11, although disruption of either gene alone causes no obvious brain malformation (Schwartzberg et al., 1991; Tybulewicz et al., 1991; Koleske et al., 1998). Here, we show that CNS-specific deletion of Abl in the Arg−/− mice results in severe cerebellar dysplasia and hypoplasia associated with the loss of the BM, which resemble cerebellar abnormalities seen in cobblestone lissencephaly. The absence of any morphological defect following granule cell-specific deletion of Abl on the same background indicates that the cerebellar phenotype is likely caused by the failure of radial glia and Bergmann glia to maintain the BM rather than a cell-autonomous defect in granule cell migration and proliferation. These findings establish Abl family kinases as essential signaling components in the regulation of BM integrity during cerebellum development.

Materials and Methods

Generation of AblF mice.

The 3′ arm of the targeting vector for generation of the floxed Abl allele contained 2.1 kb genomic sequences covering exon 7 (see Fig. 1A). The 5′ arm of the vector harbored exons 4–6 of the Abl gene, with one loxP site inserted at the end of intron 4. The neomycin resistance cassette (neo) flanked by two loxP sites was inserted into the middle of intron 6. Following homologous recombination in E14 embryonic stem (ES) cells, an expression vector of Cre recombinase (Gu et al., 1993) was transiently transfected to delete the neo cassette. Clones that retained two loxP sites flanking exons 5 and 6 were identified by Southern analysis. Two independent heterozygous ES cell clones were used to generate chimeric mice by blastocyst injection. AblF mice were mated with various mouse lines, including Arg−/− (Koleske et al., 1998), a kind gift from Tony Koleske (Yale University, New Haven, CT), EIIa-Cre (JAX #3724), Nestin-Cre (JAX #3771), Emx1-Cre (JAX #5628), and Math1-Cre (Matei et al., 2005), a kind gift from Alex Joyner (Sloan-Kettering Institute, New York, NY). ROSA26lacZ reporter line (Soriano, 1999) was obtained from The Jackson Laboratory (JAX #3474). All mutant animals were bred on a mixed 129 × C57BL/6J background. All animal procedures were approved by the Columbia University Institutional Animal Care and Use Committee (New York, NY).

Figure 1.
Generation of CNS-specific Abl knock-out mice. A, Diagram of the Abl genomic locus, the targeting construct, and the targeted alleles. The exons (open rectangles), loxP sites (red triangles), neomycin resistance cassette (neo, blue rectangle), Southern ...

Antibodies.

Mouse anti-Abl (1:1000; Calbiochem), mouse anti-α-tubulin (1:5000; Sigma), rabbit anti-Pax6 (1:500, Millipore), mouse anti-calbindin (1:5000; Swant), rabbit anti-RORα (1:50; Santa Cruz Biotechnology), rabbit anti-laminin (1:200; Sigma), rabbit anti-BLBP (1:400; Abcam), mouse anti-NeuN (1:500; Millipore), mouse anti-GFAP (1:800; Millipore), rat anti-BrdU (1:200; Abcam), rat anti-L1 (1:200; Millipore), mouse anti-TAG1 (1:4; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA), mouse anti-Crk (1:4000; BD Biosciences), rabbit anti-phospho-CrkII (Tyr221) (1:1000; Cell Signaling Technology), and mouse anti-p130Cas (1:1000; BD Biosciences).

Histological analysis and immunohistochemistry.

Embryos and postnatal mice were fixed or perfused with neutral buffered 10% formalin. Brains were either cryoprotected by 20% sucrose in PBS and embedded in OCT for frozen sections (15 μm) or embedded in paraffin for paraffin sections (7 μm). Hematoxylin and eosin (H&E) and lacZ stains were performed following standard procedures. Immunohistochemistry was performed as described previously (Qiu et al., 2010). Briefly, paraffin sections were subjected to heat-induced antigen retrieval in 10 mm sodium citrate buffer. After blocking in 10% normal donkey serum, frozen and paraffin sections were incubated with primary antibodies and then with Alexa Fluor-conjugated secondary antibodies (Invitrogen). Slides were finally mounted with Vectashield mounting medium with 4′,6-diamidino-2-phenylindole (Vector Laboratories).

BrdU labeling.

Pups were intraperitoneally injected with bromodeoxyuridine (BrdU; 100 mg/kg body weight; Sigma) and their brains were dissected after either 1 h or 4 d. For staining, sections were incubated with 2 n HCl for 30 min at 37°C before the application of antibodies.

Immunoprecipitation, active Rac1 pull-down, and Western blot.

Tissues from newborn mice were homogenized in lysis buffer (20 mm Tris, pH 7.5, 150 mm NaCl, 2 mm EDTA, 1% Nonidet P-40) supplemented with protease inhibitors (Roche). After centrifuging at 15,000 × g for 15 min, supernatant was recovered and protein concentration was determined by the Bio-Rad protein assay. Equal amounts were resolved on 8% SDS-PAGE gels and analyzed by Western blot using a standard protocol. For immunoprecipitaion, 2 mg of brain extracts were incubated with anti-Crk antibody-conjugated protein G Dynabeads (Invitrogen) and washed three times with PBS before resolution on 10% SDS-PAGE gels. Active Rac1 GTPase levels were determined using a GST-Pak1-PBD pull-down assay (Thermo Scientific) according to the manufacturer's instruction.

Cerebellar cell culture.

Granule cells and glia were purified from postnatal day (P) 4–5 cerebella according to a previously described procedure (Lee et al., 2009). Briefly, after the removal of meninges, a single-cell suspension from cerebella was obtained using the Papain Dissociation System Kit (Worthington). For granule cell proliferation assay, the cell suspension was applied to a Percoll gradient and separated by centrifugation. Enriched granule cells at the interface between the 35 and 60% Percoll gradients were cultured on 100 μg/ml poly-d-lysine (Sigma)-precoated coverslips for 2 d in serum-free medium with or without 3 μg/ml sonic hedgehog (Shh), a kind gift from Hae Young Lee, Columbia University. Four hours before fixation, cultures were treated with 10 μm BrdU. For glia-guided granule cell migration (Hatten et al., 1986), the single-cell suspension was passed through a 40 μm cell strainer and preplated on an uncoated dish to remove residual contaminating fibroblasts. The resulting granule cell and glia mixtures were cultured on 100 μg/ml poly-d-lysine/10 μg/ml laminin (Sigma)-precoated coverslips for 24 h.

Balance beam assay.

The balance beam assay of motor coordination was performed as described previously (Stanley et al., 2005). One to two-month-old mice of both genders were first trained to walk on a wide (20 mm width × 60 cm length) and then a narrow (8 mm width × 60 cm length) elevated balance beam to reach a goal platform. At the end of training trials, the mice would start to walk within a few seconds of being placed on the beam. Each test trial on the narrow beam consisted of five walks per mouse. Time to cross the beam and number of foot slips were recorded for each trial.

Statistical analysis.

Data are expressed as mean ± SEM. and were evaluated by unpaired Student's 2-tailed t test. Values were considered statistically significant at p < 0.05.

Results

Generation of CNS-specific Abl knock-out mice on the Arg−/− background

To investigate the role of Abl in CNS development, a floxed allele of Abl (AblF) was generated using Cre/loxP technology. LoxP sites upstream of exon 5 and downstream from exon 6 were introduced into ES cells by homologous recombination (Fig. 1A). Upon Cre-mediated recombination, excision of the fifth exon and sixth exon would delete part of the tyrosine kinase domain and fully inactivate the gene as determined by Western blot (supplemental Fig. S1, available at www.jneurosci.org as supplemental material, Fig. 1E), creating an Abl-null allele (AblΔ). Splicing of the primary transcript to join the flanking exons, if it occurred, would produce a frameshift mutation. AblF/F mice appeared healthy and indistinguishable from their wild-type littermates. AblF mice were first bred to males expressing an EIIa-Cre transgene (Agah et al., 1997), which deletes loxP-targeted DNAs in the germline, generating the AblΔ allele. Mice with combinations of different Abl alleles (wild-type, AblF and AblΔ) were genotyped by Southern blot (Fig. 1B) and PCR analyses (Fig. 1C) of tail DNA. Intercrosses to generate homozygotes of the AblΔ allele resulted in partial neonatal lethality with various developmental defects, such as runting, splenic, and thymic atrophy (supplemental Fig. S1, available at www.jneurosci.org as supplemental material), consistent with the previous descriptions of Abl-null mice (Schwartzberg et al., 1991; Tybulewicz et al., 1991).

CNS-specific inactivation of Abl was accomplished by crossing AblF mice to a transgenic line expressing Cre under the control of the rat nestin promoter and enhancer (Nestin-Cre) (Tronche et al., 1999). Nestin-Cre was shown to induce potent and widespread recombination in precursors of neurons and glia starting around E10 (Graus-Porta et al., 2001). Genomic DNA from multiple tissues dissected from AblF/F;Nestin-Cre or control newborns were analyzed by Southern blot analysis, confirming efficient recombination within the floxed Abl allele specifically in the brain (Fig. 1D). Furthermore, Western blot analysis of extracts from AblF/F;Nestin-Cre newborns showed nearly complete loss of Abl protein in the brain, but not in the adjacent meninges (Fig. 1E). AblF/F;Nestin-Cre mice were grossly normal and histological analysis of the brain revealed no obvious morphological defects. This was not a surprise, since no brain malformation was detected in Abl knock-out mice (Schwartzberg et al., 1991; Tybulewicz et al., 1991). We then crossed AblF/F;Nestin-Cre mice onto an Arg−/− background (Koleske et al., 1998) and generated AblF/F;Arg−/−;Nestin-Cre mice, which we refer as Abl/Arg CNS-specific double conditional knock-out (CNS-DKO) mice hereafter.

Abl/Arg deficiency in the brain results in severe cerebellar lamination defects and motor coordination deficits

Abl/Arg CNS-DKO mice were viable and recovered at a frequency (20.5%) slightly less than the expected Mendelian ratio (supplemental Table 1, available at www.jneurosci.org as supplemental material). Whole-mount analysis of the CNS-DKO young adult brain revealed profound irregularities in the cerebellum, while the rest of brain appeared normal (Fig. 2A). The phenotype was observed in all DKO mice examined, but not in their control littermates. The brains from those mice that still retained at least one functional copy of either Abl or Arg gene (e. g. AblF/+;Arg−/−;Nestin-Cre) were normal upon histological examination, indicating that the functions of the two genes are largely redundant. The DKO cerebellum was smaller in size compared with control. The anterior–posterior extent of the mutant cerebellum, with the disappearance of lobules IV and V in the anterior vermis (Fig. 2A, arrows), was markedly reduced. Furthermore, both the cerebellar vermis and the two lateral hemispheres lacked clear fissures on the surface.

Figure 2.
Abl/Arg deficiency in the brain results in cerebellar malformations and motor coordination deficits. A, Pictures of whole brains dissected from adult Abl/Arg CNS-DKO mouse and its control littermate (CTR) showing the gross abnormalities in the DKO cerebellum. ...

Histological analysis of the DKO brain revealed proper lamination of the cerebral cortex and hippocampus (supplemental Fig. S2, available at www.jneurosci.org as supplemental material). However, sagittal and coronal sections of the DKO cerebellum not only confirmed that its length along the anterior–posterior axis was dramatically reduced, but also revealed several other striking abnormalities (Fig. 2B–G). First, the basic laminar structure of the anterior cerebellum, encompassing lobules I–V, was completely lost in the DKO mice. As a result, patches of granule cells were randomly scattered throughout the white matter. Second, although the basic foliation pattern of the hemispheres and the posterior cerebellum seemed to be preserved (supplemental Fig. S3, available at www.jneurosci.org as supplemental material), adjacent lobules, such as lobules VIII and IX, were fused and the depth of the fissures was also significantly decreased (Fig. 2F,G). Moreover, while a rudimentary IGL formed, many granule cells failed to migrate and formed ectopias at the cerebellar surface and along the fusion lines of adjacent lobules (Fig. 2F,G, arrows). Together, the histological analysis demonstrates that the development of cerebellar cortex is specifically compromised in the absence of Abl and Arg, leading to anterior cerebellar morphogenesis defects and cortical lamination abnormalities.

Considering the profound morphological defects in the cerebella of Abl/Arg CNS-DKO mice, we examined motor coordination function in these mutants. The DKO mice did not display obvious ataxia in an open field test. However, when placed on an elevated balance beam, differences between the DKO mice and their control littermates were evident (supplemental movies S1, S2, available at www.jneurosci.org as supplemental material): DKO mice took approximately twice as long to cross the beam (Fig. 2H) and also demonstrated a dramatic increase in the number of foot slips (Fig. 2I). Thus, the DKO mice exhibit substantial motor coordination deficits that do not affect normal locomotor function but becomes apparent upon challenge with more complicated tasks. Like the cerebellar malformation, poor motor coordination was also observed only in the DKO mice, suggesting that the impairment of motor function is probably caused by cerebellar defects.

Disruption of the BM during embryonic development leads to morphogenesis defects in the anterior cerebellum

To determine the developmental stage at which the malformation of the anterior cerebellum arises, we examined Abl/Arg CNS-DKO mice at younger ages (Figs. 3, ,4).4). Sagittal sections of P7 and P1 cerebella showed obvious defects in the organization of anterior lobules I–V in the DKO mice (Fig. 3A–D). Immunostaining of P1 sections with markers for granule cells (Pax6) and Purkinje cells (calbindin) revealed that both cell types were mislocalized: many GCPs were ectopically embedded within the cortex and Purkinje cells were loosely aligned, with some abnormally distributing at the cerebellar surface (Fig. 3E,F). At E17, while RORα-positive Purkinje cells formed a normal multilayer below the EGL (Fig. 3G), GCPs appeared more dispersed in the anterior DKO cerebellar primordium when compared with control (Fig. 3G). A close examination revealed that laminin-labeled BM was fragmented, and scores of GCPs protruded into the adjacent subarachnoid space between the midbrain and cerebellum in the DKO embryos (Fig. 3H, arrows). These observations suggest that the mislocalization of Purkinje cells (supplemental Fig. S4, available at www.jneurosci.org as supplemental material, Fig. 3F) in postnatal DKO mice is likely a consequence of the anterior cerebellar malformation involving defective BM and abnormally distributed granule cells.

Figure 3.
Morphogenesis defects in the anterior cerebellum of Abl/Arg CNS-DKO mice. AF, Abl/Arg CNS-DKO mice showed foliation defects in the anterior cerebellum at early postnatal stages. AD, H&E staining of P7 and P1 control (CTR) and ...
Figure 4.
Disruption of the BM during embryonic development in Abl/Arg CNS-DKO mice leads to morphogenesis defects in the anterior cerebellum. AD, Abnormal radial glial endfeet and breaches of the BM precede the arrival of granule cell precursors in the ...

To probe whether loss of the BM integrity is caused by aberrant tangential migration of GCPs, we analyzed sections from earlier embryos. At E15, while the migrating GCPs had not yet arrived at the anterior cerebellum, there were already multiple small discontinuities in the BM in the DKO mice (Fig. 4B,D′, arrows), suggesting that the BM is not disrupted by migrating GCPs. During early CNS development, radial glial processes extend from the ventricular zone to the cortical surface, where their endfeet are the preferred site of BM assembly (Halfter et al., 2000). Therefore, the failure of radial glia to maintain the BM may potentially cause its breach. Indeed, we found that the BM in the anterior cerebellum at E15 serves as the primary anchorage site for dense brain lipid-binding protein (BLBP)-positive radial glial processes (Fig. 4C,C′). In the DKO cerebellum, radial glial processes were highly disorganized, with some of their endfeet protruding into the meninges (Fig. 4D,D′, asterisk). Since the Nestin-Cre transgenic mice did not induce recombination in the ECM-secreting meningeal fibroblasts (Fig. 1E), these data provide strong evidence that Abl family kinases in radial glia is likely required for BM integrity. In conclusion, our time course analyses demonstrate that the abnormalities in the BM in Abl/Arg CNS-DKO embryos, likely caused by altered radial glia-BM interactions, leads to mislocalization of tangentially migrating GCPs and subsequent foliation defects in the anterior cerebellum.

Abnormal Bergmann glial network and defective BM maintenance in the postnatal cerebellum

During postnatal cerebellar development, GCPs in the EGL proliferate and give rise to differentiated granule cells that migrate radially along the Bergmann glial fibers and then through the PCL to form the IGL (Hatten, 1999; Sillitoe and Joyner, 2007). By P21, the migration of granule cells is complete and the EGL is no longer present, leaving behind the molecular layer (ML). Staining of a postmitotic neuronal marker NeuN showed frequent granule cell ectopias in the posterior regions of adult Abl/Arg CNS-DKO cerebella, where the basic foliation pattern was generally intact (Fig. 5A–D). Ectopic granule cells were predominately located at the cerebellar surface and along the fused fissures (Fig. 5B, arrow). This distribution correlated with regions in which the loss of the pial BM starting from around P8 (Fig. 5F, arrows). In control cerebellum, GFAP-labeled Bergmann glia extended processes across the ML, and their endfeet were anchored along the BM (Fig. 5G,I). In contrast, the glial fibers in the DKO mice were highly disorganized, with many random protrusions at the cerebellar surface (Fig. 5H,J). Since the interaction with the endfeet of Bergmann glial fibers has been suggested to be critical for maintenance of the BM in the postnatal cerebellum (Graus-Porta et al., 2001; Belvindrah et al., 2006), our results indicates that, similar to the defect in the DKO cerebellar primordium, the disruption of the BM in the postnatal cerebellum may likely also result from the abnormal Bergmann glial network.

Figure 5.
Abnormal Bergmann glial network and defective BM maintenance in the postnatal cerebellum of Abl/Arg CNS-DKO mice. AD, NeuN staining of adult control (CTR) and DKO cerebellar sections showing granule cell ectopias (B, arrow) at the cerebellar ...

GCP proliferation and differentiation in Abl/Arg CNS-DKO mice

With the formation and growth of the lobules, the size of the postnatal mouse cerebellum increases dramatically, which is largely caused by the proliferation of GCPs. The smaller cerebella with reduced foliation in the posterior cerebellum in the DKO mice (Fig. 2C) indicate a deficiency in GCP proliferation. To directly assess proliferative defects, we performed 1 h BrdU pulse-labeling experiments at P8 during the peak stage of GCP proliferation (supplemental Fig. S5, available at www.jneurosci.org as supplemental material, Fig. 6A,B). Because the secondary fissure is the major fissure in the posterior cerebellum, the initial formation of which is normal in the DKO mice (supplemental Fig. S5, available at www.jneurosci.org as supplemental material and Fig. 6B), we quantified the number of proliferating GCPs in it and found a significant reduction in the DKO mice (311 ± 17 BrdU-positive cells/mm; n = 3 mice for each genotype) compared with controls (405 ± 25 BrdU-positive cells/mm). Furthermore, loss of proliferating GCPs seemed to be concentrated in areas where the BM was disrupted (Fig. 6B, arrows). Since an intact BM is important for maintaining the proliferative capacity of GCPs (Blaess et al., 2004; Belvindrah et al., 2006), these data suggest that impaired granule cell proliferation may not be a cell-autonomous defect. To test this hypothesis, we isolated granule cells from control and DKO pups and cultured them for 48 h in medium with or without 3 μg/ml Shh, which is a potent mitogen for GCPs and regulates the growth and elaboration of the cerebellar lobules (Corrales et al., 2006). Before fixation, cells were labeled with BrdU and the prevalence of BrdU-positive cells was determined (Fig. 6C–G). Consistent with previous studies (Wechsler-Reya and Scott, 1999), the vast majority of GCPs derived from control mice stopped proliferating in the absence of Shh, and the addition of Shh strongly promoted GCP proliferation. A similar response in proliferation of GCPs from the DKO mice was also observed (Fig. 6G), indicating that Abl kinases are not required for the proliferation of GCPs in response to Shh in vitro. This result further suggests that the decreased granule cell proliferation in the DKO mice is likely secondary to defects in the BM.

Figure 6.
GCP proliferation and differentiation in Abl/Arg CNS-DKO mice. A, B, Decreased GCP proliferation in Abl/Arg CNS-DKO mice. P8 mice were injected with BrdU and killed 1 h later, and BrdU and laminin costaining (A, B) of their cerebellar secondary fissure ...

During normal differentiation, granule cells exit the cell cycle, enter the inner EGL, and spontaneously express differentiation markers such as the adhesion molecules L1 and TAG1 (Fig. 6H,J). In the DKO mice, however, with focal GCPs losing contact with the BM, they exited the cell cycle (Fig. 6B) and ectopically expressed L1 and TAG1 at the cerebellar surface (Fig. 6I,K). This might result in a depletion of the precursor pool within the EGL and likely contribute to the cerebellar hypoplasia in the DKO mice.

Granule cell migration in Abl/Arg CNS-DKO mice

The observation that granule cell ectopias formed at sites of broken BM in the postnatal DKO cerebellum (Fig. 5) raises the possibility that the phenotype is secondary to abnormalities in the BM and is not directly caused by intrinsic migration defects of Abl/Arg-deficient granule cells. To evaluate the rate of granule cell migration, we labeled proliferating GCPs with a single BrdU injection at P6 and analyzed their extent of migration at P10 before the extensive loss of the BM in the DKO mice (Fig. 7A,B). As expected, the majority of BrdU-labeled cells (90.9 ± 2.7%; n = 3 mice for each genotype) were found in the IGL in lobule VIII in control mice. A similar percentage (85.3 ± 4.0%) was also observed in the same lobule in the DKO cerebella, except in few regions where the integrity of the BM was already destroyed and clusters of BrdU-labeled granule cells remained at the cerebellar surface (data not shown). This provides strong evidence that the intrinsic program for cerebellar granule cell migration is not perturbed in the DKO mice.

Figure 7.
Granule cell migration in Abl/Arg CNS-DKO mice. A, B, Normal granule cell migration before the extensive loss of the BM in Abl/Arg CNS-DKO mice. P6 mice were injected with BrdU and killed 4 d later, and their cerebellar sections were then stained with ...

To investigate this further, we examined granule cell–glia interactions both in vivo and in vitro. The attachment of migrating granule cells to Bergmann glial fibers was readily detected in both control and DKO cerebellar sections (Fig. 7C,D). The close interaction was also evident in cultures derived from both control and DKO mice in which many of the granule cell bodies assumed an elongated shape, indicating that they were migrating (Fig. 7E,F). Together, these results demonstrate that Abl family kinases are not essential in either granule cell or glia for their interactions.

Granule cell-specific deletion of Abl family kinases is not sufficient to induce a cerebellar lamination defect

To further determine whether Abl family kinases in granule cells are essential for cerebellar lamination and their proliferation, we generated conditional knock-out mice in which Abl was specifically deleted from granule cells using a Math1-Cre transgenic line (Matei et al., 2005). Math1-Cre was characterized by crossing with a ROSA26lacZ reporter line (Soriano, 1999). As shown by lacZ staining, Math1-Cre drives efficient recombination in all GCPs except those in the most posterior part of the cerebellum (Fig. 8A,B). Western blot analysis of cerebellar extracts from AblF/F;Math1-Cre pups showed that the level of Abl protein was markedly decreased when compared with that in control mice (Fig. 8C). The residual Abl protein detected probably reflects the protein present in other Cre-negative cells such as Purkinje cells and glia in the cerebellar tissue. When AblF/F;Math1-Cre mice were crossed into the Arg knock-out background, the cerebella of the resulting mutant mice were strikingly normal, with no malformation or granule cell ectopia observed (Fig. 8D,E). Although we cannot rule out the possibility that Abl protein is not completely deleted in granule cells by Math1-Cre, this result provides further genetic evidence that granule cells are not the primary cause of the cerebellar lamination defects observed in Abl/Arg CNS-DKO mice.

Figure 8.
Granule cell-specific deletion of Abl family kinases is not sufficient to cause cerebellar lamination defect. A, B, LacZ staining of E18 (A) and P21 (B) cerebellar sections from the ROSA26lacZ reporter mice showing granule cell-specific recombination ...

Analysis of signaling pathways regulated by Abl family kinases

In fibroblasts, integrin-mediated attachment on the ECM triggers the tyrosine phosphorylation of intracellular signaling proteins, including Abl family kinases and the adaptor protein CrkII, and leads to the rearrangement of the actin cytoskeleton (Bradley and Koleske, 2009). Interestingly, among other defects, mice lacking Crk family members in the brain also showed similar granule cell ectopias as observed in Abl/Arg CNS-DKO mice (Park and Curran, 2008). CrkII binds to the PxxP motifs of Abl family kinases through its SH3 domain (Antoku et al., 2008) and is phosphorylated by them at residue Y221 (Feller et al., 1994). When Y221 is phosphorylated, CrkII forms an intramolecular interaction with its SH2 domain, which blocks its activity as an adaptor protein and prevents its interaction with a key scaffold molecule, p130Cas (Kain and Klemke, 2001). While it was unchanged in Arg−/− newborn brain extracts, phosphorylation of CrkII Y221 was significantly reduced in AblF/F;Nestin-Cre extracts and almost completely abolished in Abl/Arg CNS-DKO extracts (Fig. 9A). This indicates that CrkII phosphorylation at Y221 in the brain is primarily dependent on Abl and, to a lesser extent, on Arg. The diminished phosphorylation of CrkII Y221 also correlated with the increased association of p130Cas with Crk in the DKO extracts (Fig. 9B). Although how Abl-mediated CrkII phosphorylation affects Rac1 activity is dependent on different biological contexts (Bradley and Koleske, 2009), the overall level of active Rac1 GTPase was elevated in the DKO brain extracts (Fig. 9B).

Figure 9.
Crk signaling is altered in Abl/Arg-deficient brain. A, Immunoprecipitation (IP) of brain extracts from newborns with the indicated genotypes using anti-Crk antibody followed by Western blot with antibodies to phospho-Y221 (p-Y221), CrkII, and Crk. Western ...

Discussion

In this study, we examined the function of Abl family kinases in brain development by generation of a new conditional Abl knock-out allele. Analysis of mutant mice lacking the two kinases in the CNS reveals that Abl and Arg are both critically required for the maintenance of a normal BM in the cerebellum. Abl/Arg deficiency results in abnormal radial glial and Bergmann glial networks and local BM disruptions that have profound impacts on cerebellar development.

Our results demonstrate that Abl/Arg deficiency in the brain results in local breaches in the cerebellar BM at different developmental stages, including at E15 in the anterior cerebellar primordium (Fig. 4) and after P8 in the rest of the cerebellum (Fig. 5). The loss of BM leads to severe anterior cerebellar morphogenesis defects and correlates with abnormal postnatal granule cell proliferation, migration, and differentiation. A similar phenotype in brain development was reported in mice lacking laminin receptors α-dystroglycan and β1 integrin as well as two integrin-associated signaling proteins, FAK and ILK (Graus-Porta et al., 2001; Moore et al., 2002; Beggs et al., 2003; Niewmierzycka et al., 2005; Belvindrah et al., 2006; Mills et al., 2006; Satz et al., 2008). It has been proposed that the normal anchorage of radial glial endfeet and laminin binding-triggered signaling cascades in glia are important for organizing extracellular laminin and thereby maintaining the BM (Fig. 9C) (Colognato et al., 1999; Henry et al., 2001; Beggs et al., 2003). In Abl/Arg CNS-DKO mice, the fiber networks of cerebellar radial glia and Bergmann glia are both disorganized and their endfeet protruded beyond the pial surface, coinciding with regions of discontinuous BM. These observations suggest that Abl family kinases are probably required in radial glia and Bergmann glia to stabilize the BM through laminin binding-triggered signaling pathways (Fig. 9C). Consistently, time course analyses indicate that the abnormal tangential migration of GCPs into the adjacent subarachnoid space between the midbrain and cerebellum is secondary to disruptions in the BM in the DKO embryos. Moreover, we did not observe obvious defects in radial migration of postnatal granule cells before the loss of the BM or abnormal granule cell-glia interactions either in sections or in vitro culture (Fig. 7). Genetic evidence that lacking Abl kinases in cerebellar granule cells alone does not cause any lamination defect further supports a noncell-autonomous role for Abl family kinases in granule cell migration and proliferation (Fig. 8). Although we still cannot exclude the possible contributions from other cerebellar neurons, such as Purkinje cells, deletion of Abl/Arg from glia is likely required for the DKO phenotype.

Unlike α-dystroglycan or β1 integrin mutants in which the development of both cerebral cortex and cerebellum is affected, the lamination abnormalities of Abl/Arg-deficient mice are restricted to the cerebellum even though Abl kinases are ubiquitously expressed (Koleske et al., 1998) and the deletion of Abl by Nestin-Cre is throughout the brain (Fig. 1E). This variation could be explained by the utilization of Cre lines with different expression timing or efficiency. For example, Nestin-Cre-mediated inactivation of ILK results in only cerebellar malformations (Belvindrah et al., 2006; Mills et al., 2006), while deletion of the same gene by dorsal forebrain-specific Emx1-Cre leads to cerebral cortical defects (Niewmierzycka et al., 2005). The difference may be attributable to the slightly earlier activation of Emx1-Cre (around E9) in precursors of neurons and glia compared with that of Nestin-Cre (around E10) (Graus-Porta et al., 2001; Gorski et al., 2002). But this seems not to be the case for Abl kinases, because AblF/F;Arg−/−;Emx1-Cre mice still develop a normal forebrain cortex (data not shown). This argues that other kinases, such as FAK and ILK, can substitute for the function of Abl and Arg in stabilizing the BM in the brain, with the exception of the cerebellum. Interestingly, during development the cerebellum undergoes a dramatic expansion in its volume along with a unique postnatal foliation process (Sillitoe and Joyner, 2007), which might cause a prolonged and consistent mechanical pressure on its BM such that it becomes more susceptible to the loss of Abl family kinases than other brain regions.

The morphogenesis defect in the anterior cerebellum is striking and has 100% penetrance. The small disruptions of the BM in the anterior cerebellar primordium are the first detectable defects that precede the mislocalization of GCPs and failed foliation. The BM in the anterior cerebellum at E15 seems to be the major site for the attachment of radial glial endfeet (Fig. 4). Why it is particularly fragile during development in Abl/Arg CNS-DKO mice is still not clear. The defect is unique to Abl/Arg-deficient mice and is not observed in mutants lacking α-dystroglycan or β1 integrin in the brain (Graus-Porta et al., 2001; Moore et al., 2002). One possible explanation is that Abl family kinases are required for mediating signaling downstream of the engagement of both α-dystroglycan and β1 integrins in cerebellar radial glia to promote the ECM stability. Thus loss of Abl and Arg could result in a more severe phenotype in the cerebellar primordium than mutation in either α-dystroglycan or β1 integrin alone. Alternatively, Abl family kinases may be involved in signaling mediated by other unknown ECM receptors during early cerebellar development. Recently, it was reported that a mouse mutation in an orphan G protein-coupled receptor, GPR56, whose loss-of-function causes human bilateral frontoparietal polymicrogyria, results in a very similar anterior cerebellar malformation as seen in Abl/Arg CNS-DKO mice (Koirala et al., 2009). Although it has been proposed that GPR56 regulates BM integrity as a putative ECM receptor in radial glial endfeet (Li et al., 2008), GPR56 appears to specifically express in granule cells in the anterior cerebellum and controls their adhesion to the ECM (Koirala et al., 2009). The question of whether Abl family kinases are implicated in GPR56-mediated signaling merits further investigation.

How do Abl family kinases regulate BM integrity in radial glia and Bergmann glia? Since Abl family kinases are important regulators of the actin cytoskeleton downstream of β1 integrins (Bradley and Koleske, 2009), deletion of Abl and Arg may prohibit the cytoskeletal rearrangement and signaling pathways that are required to transduce the bidirectional signals necessary for laminin polymerization and overall BM stability (Fig. 9C). Abl-mediated Crk Y221 phosphorylation has been implicated in the regulation of multiple biological processes, including cell migration (Kain and Klemke, 2001; Noren et al., 2006), cell–cell adhesion (Zandy et al., 2007), and bacterial invasion (Burton et al., 2003). Although how Crk phosphorylation modulates the actin cytoskeleton is not fully understood (Bradley and Koleske, 2009), perturbation of the adaptor protein function could play a significant role in the DKO mutant phenotype (Fig. 9A,B). However, because loss of Crk Y221 phosphorylation is found throughout the brain in the DKO mice, but the mutant phenotype is confined to the cerebellum, this suggests that other Abl/Arg-dependent mechanisms are required in the cerebellum for BM maintenance.

The severe cerebellar displasia and hypoplasia of Abl/Arg CNS-DKO mice resemble cerebellar defects seen in some forms of congenital muscular dystrophy, particularly Walker-Warburg Syndrome, the majority of which have an unknown genetic defect (Muntoni and Voit, 2004). This suggests that Abl family kinase-mediated signaling may be perturbed in these diseases and, consequently, could play a role in their pathogenesis, although the expression levels of glycosylated α-dystroglycan seemed to be unaffected in Abl/Arg-deficient brain (data not shown). Future studies will clarify the role of Abl family kinases in this group of diseases.

Footnotes

This work was supported by National Cancer Institute Grant P01 CA023767 (S.P.G.). We thank Tony Koleske for Arg−/− mice, Alex Joyner for Math1-Cre mice, Hae Young Lee for recombinant Shh, Carol Mason, Alex Joyner, Hynek Wichterle, and Mary Hatten for advice, Shahin Aeinehband, Hae Young Lee, Yu Shi, Alex Rebsam, and Turgay Akay for technical assistance, and members of the laboratory for helpful discussions.

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