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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Hum Genet. Author manuscript; available in PMC 2013 November 21.
Published in final edited form as:
PMCID: PMC3836273
NIHMSID: NIHMS525275

The SCARB1 gene is associated with lipid response to dietary and pharmacological interventions

Abstract

The scavenger receptor class B type 1 (SCARB1) gene is a key component in the reverse cholesterol transport pathway and thus plays an important role in lipid metabolism. Studies suggested that the SCARB1 gene may contribute to variation in plasma lipid levels at the fasting; however, the results have been inconsistent and it is unclear if SCARB1 may also influence lipid response to dietary and pharmacologic interventions. In this study, we examined genetic variation in the SCARB1 gene in participants of the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study for associations with basal lipid levels, changes in lipid measures after dietary fat intake and fenofibrate treatment. We found that the exon 1 variant SCARB1_G2S was significantly associated with post-fenofibrate change for triglyceride (TG) (P = 0.004). Subjects bearing SCARB1_G2S minor allele A tend to have higher responsiveness to fenofibrate in lowering TG. In summary, our study suggested that the SCARB1 gene may serve as a useful marker that predicts variation in baseline lipid levels, postprandial lipid response as well as response to fenofibrate intervention.

Keywords: Scavenger receptor class B type 1, lipid, genetics, fenofibrate, postprandial

INTRODUCTION

Plasma triglyceride (TG) and cholesterol levels are major risk factors of coronary heart disease (CHD) (Cullen et al. 1997). High-density lipoprotein cholesterol (HDL-C) levels inversely correlated with CHD risk, suggesting HDL-C protects against atherosclerosis (Gordon and Rifkind 1989; Uiterwaal et al. 1994). TGs, LDL and HDL particles and particle size are highly reproducible, and are sensitive to dietary manipulation and lipid-lowering drugs (Tsai et al. 1992; Yuan et al. 1994). Studies showed that both the fasting lipid levels and the postprandial lipid response are modulated by genetic factors (Mero et al. 1998; Ordovas 2001). In addition, several lines of evidence suggested that lipid response to pharmacological interventions is highly variable and has genetic control (Suter et al. 2001; Durstine et al. 2001; Kuller et al. 2001). However, these underlying genetic factors are largely unknown.

The scavenger receptor class B type 1 (SCARB1) gene is the first reported HDL receptor (Acton et al. 1996). SCARB1 mediates selective uptake of HDL-C without degradation of entire HDL particles (Acton et al. 1996). Over-expression of SCARB1 in mice resulted in dramatic reductions in plasma HDL-C concentrations (Kozarsky et al. 1997). Conversely, suppression of SCARB1 expression were related to a marked increase in plasma HDL-C, characterized by enlarged cholesterol-rich HDL particles and impaired HDL-C clearance (Rigotti et al. 1997). As a multilipoprotein receptor, SCARB1 also regulates the concentrations of LDL-C and very low density lipoprotein (VLDL) (Acton et al. 1996; Ueda et al. 1999). Interestingly, SCARB1 is likely to be involved in intestinal absorption of TGs (Hauser et al. 1998). Importantly, the SCARB1 gene is located on chromosome 12q24, a region showing significant linkage to plasma HDL-C and TG levels (Feitosa et al. 2005). Several previous studies also reported associations between the SCARB1 gene polymorphisms and plasma cholesterol levels in humans; however, the results have been inconsistent (Acton et al. 1999) and it is unclear whether the SCARB1 gene is involved in modulating postprandial lipid response and response to pharmacological interventions.

Stimulated by the importance of SCARB1 in lipid metabolism, in this study we systemically investigated whether the SCARB1 gene contributes to baseline lipid levels, lipid response to dietary fat challenge and, for the first time, TG lowering drug therapy in participants of the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study.

MATERIALS AND METHODS

Study population

The study population came from the GOLDN study (http://www.biostat.wustl.edu/goldn/). The participants of GOLDN study were mainly re-recruited from two NHLBI Family Heart Study (FHS) field centers: Minneapolis, MN, and Salt Lake City, UT. Nearly all subjects were of European ancestry. Eligibility criteria were: 1) ≥ 18 years of age; 2) fasting TGs < 1500 mg/dL; 3) willing to participate in the study and attend the scheduled clinic exams; 4) member of a family with at least two members in a sibship; 5) AST and ALT results within normal range; and 6) creatinine ≤ 2.0 mg/dL. Exclusion criteria were: 1) history of liver, kidney, pancreas, gall bladder disease, or malabsorption; 2) current pregnancy; 3) insulin use; 4) use of lipid lower drugs (including prescription, OTC and nutraceuticals; volunteers taking these agents were withdrawn from them at least four weeks prior to the study with physician’s approval; 5) use of warfarin; 6) women of childbearing potential not using an acceptable form of contraception; 7) known hypersensitivity to fenofibrate; and 8) history of pancreatitis within 12 months prior to enrollment.

Our previous studies demonstrated that Caucasians in UT and MN were homogeneous and thus pooling data across centers will not threaten the validity of this study (Pankow et al. 2002).

Dietary and pharmacological Interventions

All family members underwent a baseline screening visit (Visit 0). The eligible subjects were asked to participate in a clinical examination with dietary fat intake and the fenofibrate trial. The dietary fat challenge was conducted to assess baseline and postprandial TGs and related phenotypes. The fat challenge consisted of eating a test meal during a 15-minute period and returning to the clinic 3.5 and 6 hrs later to collect blood samples. The caloric intake of the intervention meal was determined by body surface area. The meal derived 83% of calories from fat and contains 700 calories per m2 of body surface area. We measured the total TGs, TG in chylomicrons, VLDL and TG remnant particles, HDL and LDL particle sizes, total cholesterol, LDL-C, and HDL-C at baseline and at 3.5 and 6 hrs after consumption of the meal.

This fat challenge was done twice, once while participants were taking no lipid-lowering medications, and once while taking fenofibrate. The fenofibrate intervention consisted of a three-week treatment period, in which participants took oral fenofibrate 160 mg tablets once daily. We measured lipids twice after a minimum fast of 10 hours on the last two days of the treatment period (Visits 3 and 4). The intervention procedure is displayed in Supplementary data (supplementary Figure 1).

Plasma lipid and lipoprotein measurements

The fasting, 3.5, and 6 hr postprandial lipoprotein samples were centrifuged within 20 min of collection at 2000g for 15 min at 4 °C. Plasma samples of each participant were stored at 4°C until completion of the treatment period. All samples from a single participant were analyzed within the same batch. TGs were measured using a glycerol blanked enzymatic method (Trig/GB, Roche Diagnostics Corporation, Indianapolis, IN) on the Roche/Hitachi 911 Automatic Analyzer (Roche Diagnostics Corporation). Cholesterol was measured on the Hitachi 911 using a cholesterol esterase, cholesterol oxidase reaction (Chol R1, Roche Diagnostics Corporation). The same reaction was also used to measure HDL-C after precipitation of non-HDL-C with magnesium/dextran. LDL-C was measured by a homogeneous direct method (LDL Direct Liquid Select Cholesterol Reagent, Equal Diagnostics, Exton, PA) on the Hitachi 911. TGs were measured at all time points, i.e., 0, 3.5, and 6 hrs before/after fenofibrate trial. HDL-C and LDL-C were only measured at baseline before and after fenofibrate exposure.

Plasma lipoprotein particles and subclass distributions were determined by proton nuclear magnetic resonance (NMR) spectroscopy as previously described (Ordovas et al. 2000; Otvos et al. 1992). Each profile displays the concentrations of 3 VLDL, 1 IDL, 3 LDL, and 3 HDL subclasses and the weighted-average particle sizes of VLDL, LDL, and HDL. The lipoprotein subclass categories used were the following: large LDL (21.3 to 27.0 nm), intermediate LDL (19.8 to 21.2), small LDL (18.3 to 19.7 nm), large HDL (8.8 to 13.0 nm), intermediate HDL (7.8 to 8.8 nm), and small HDL (7.3 to 7.7 nm). Levels of LDL and HDL subclasses are expressed in nmol units of cholesterol. LDL and HDL subclass distributions determined by gradient gel electrophoresis and NMR have been shown to be closely correlated (Otvos et al. 1992). HDL, LDL, VLDL particles and subclass were measured at all time points (0, 3.5 and 6 hrs) before/after fenofibrate trial.

SNP Selection and Genotyping

SNPs were identified by searching public databases such as dbSNP (http://www.ncbi.nlm.nih.gov/SNP/). We selected 7 SNPs at the SCARB1 gene locus based on the following criteria, in order of importance in our selection scheme: 1) validation status; 2) functional relevance and importance; 3) degree of heterozygosity, i.e., minor allele frequencies (MAF) > 0.0; and 4) previous evidence of association with lipid measurements.

SNPs were genotyped by using the 5′ nuclease allelic discrimination Taqman assay with allelic specific probes on the ABI Prism 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA) according to standard laboratory protocols. We used GRR software (Abecasis et al. 2001) to detect pedigree errors via graphically inspecting the distribution for marker allele sharing among pairs of family members or all pairs of individuals. The SNP allele frequencies were estimated via a maximum-likelihood method (Boehnke 1991). For each SNP, a χ2 test was used to examine deviation of SNP genotypes from Hardy-Weinberg equilibrium (HWE) (Fisher RA 1934). The overall genotyping error and missing rate was ~1%.

Statistical analyses

Pairwise linkage disequilibrium (LD) between SNPs was calculated using the normalized measure, Lewontin’s D, (LEWONTIN 1964), using the publicly available HAPLOVIEW version 3.3 software (Barrett et al. 2005). Normality for continuous variables was checked. After logarithmic transformations for TGs, HDL-C and HDL particle size, they approximately follow normal distribution.

Associations were tested between the SCARB1 SNPs and a number of lipid profiles, which include: 1) baseline lipid levels (including TGs, HDL-C, LDL-C, HDL and LDL particles and particle sizes); 2) changes of lipid profile between pre- and post-fenofibrate treatments; 3) changes of lipid profile at 0–3.5 hr (which, biologically, represents the absorption phase of TGs after fat meal); and 4) changes of lipid profile at 3.5–6 hr (which, biologically, represents the clearance phase of TGs after fat meal).

Genotype-phenotype association analyses were performed using a linear mixed model that is implemented in SAS (version 9.1, SAS Institute, Cary, NC). In the model, genotypes were treated as fixed effects and the dependencies among members within each family were treated as random effects. Age, age2, age3, sex, and field center were included as covariates. The model can be defined as:

equation M1

where yit is the phenotype of individual i measured at time point t. α and β are the intercept and slope for the population level effects (fixed effects) respectively, while γ and γ are for the individual level effects (random effects) respectively. εit is the residual. The model was implemented in the PROC MIXED procedure in SAS. Since the growth curve slope (β +γ) eliminates the noise εit, it represents an accurate and stable estimate of lipid changes.

Haplotypes were reconstructed based on the 7 SNPs. The software MERLIN version 1.0.1 (Abecasis et al. 2002) was used for haplotype inference. MERLIN can accommodate LD between SNPs in haplotype inference (Abecasis and Wigginton 2005). Association analysis for haplotypes was conducted using a mixed model similar to that for single SNPs. In addition, a global test was performed to examine whether phenotype differences existed among all haplotypes simultaneously.

RESULTS

Study population

Characteristics of the study subjects are shown in Table 1. A total of 1,327 subjects (639 men and 688 women) from 148 families were genotyped. Of these 1,327 subjects, 861 subjects (427 men and 434 women) went through the fenofibrate trial and had complete lipid phenotypes (TGs, HDL-C and LDL-C) and genotype data. Men ranged in age from 18.0 to 87.6 (50.6 ± 15.9) yr, and women ranged from 18.0 to 87.2 (51.1 ± 15.8) yr. For both men and women, at all the time points, TG, LDL-C, and LDL particles after fenofibrate treatment were significantly lower than those before fenofibrate treatment (P <0.001). Conversely, HDL-C and HDL particles after fenofibrate treatment were significantly higher than those before fenofibrate treatment (P < 0.001). Post-fenofibrate HDL sizes generally increased, compared to those before fenofibrate treatment at each corresponding time point for both men and women. The changes of VLDL and LDL particle sizes before/after treatment were not in a consistent pattern for both men and women.

Table 1
Characteristics of study subjects

SNP genotyping and linkage disequilibrium

Information of the 7 tested SNPs is summarized in Supplementary Table 1. All the SNPs were in Hardy-Weinberg equilibrium. The allele frequencies of all the SNPs were greater than 0.10. LD coefficients (Lewontin’s D′) between each pair of the SNPs are shown in Supplementary Table 2. We found substantial LD (D′ ≥ 0.50) among several pairs of SNPs. The strongest LD (D′ = 0.904) was found between SCARB1_I51973 (intron 5) and SCARB1_A350A (exon 8).

Association analyses for single SNPs

Table 2 summarizes the association results between SNPs and lipid response to fenofibrate treatment in the total sample. Overall, fenofibrate was associated with a 27.2 mg/dl reduction in TG level. The SNP SCARB1_G2S was associated with fenofibrate response for both TG (P = 0.004) and HDL-C (P = 0.01). The SNP SCARB1_I82699 was associated with fenofibrate response for TG (P = 0.02). Figure 1 displays the changes of HDL-C and TG for SCARB1_G2S genotypes. It can be seen that subjects bearing the allele A had larger changes for both HDL-C and TG, suggesting higher response to fenofibrate treatment by increasing plasma HDL-C level and lowering TG level.

Figure 1
Changes of HDL-C and TG with SCARB1_G2S genotypes in response to fenofibrate treatment. Changes (mean ± SE) of HDL-C and TG (Y axis) were growth curve slopes estimated from the mixed models.
Table 2
Association of SCARB1 SNPs with lipid response to fenofibrate treatment *

Table 3 summarizes the association results of the lipid measures at the baseline (0 hr) and the lipid changes across time points of 0–3.5 hr (0/3.5) and 3.5–6 hr (3.5/6). The most pronounced association was found for the SNP SCARB1_G2S. In the pre-fenofibrate phase, a significant association was found between SCARB1_G2S and baseline HDL (P = 0.001), HDL0–3.5 (P = 0.04), and changes of HDL particle size at time period 0–3.5 hr (P = 0.03). SCARB1_G2S also showed associations with baseline TG (P = 0.004), TG0–3.5 (P = 0.005), and baseline LDL (P = 0.03) (see Table 3).

Table 3
Association tests of SCARB1 SNPs with baseline lipid measures and response to dietary fat *

For post-fenofibrate lipid measures, significant associations were observed for SCARB1_G2S with baseline HDL particles (P = 0.002), HDL3.5–6, and HDL size at time period of 3.5–6 hr (P = 0.0002). However, no association was found between SCARB1_G2S and baseline TG or TG response to fat loading.

Association analyses for haplotypes

A total of 51 haplotypes were observed in our family data. To maximize the statistical power and to minimize potential bias caused by small number of subjects bearing a specific haplotype, we only tested associations between common haplotypes (allele frequencies ≥ 0.05) and lipid measures. Table 4 illustrates the frequencies and association information of the 6 common haplotypes (H1–H6). No significant association was observed between any single haplotype with response to fenofibrate treatment. However, the haplotype H1 showed significantly association with pre-fenofibrate baseline HDL (P = 0.002) and marginally association with HDL3.5–6 (P = 0.07). H1 was also association with pre-fenofibrate TG0–3.5 (P = 0.02). The haplotype H2 was associated with post-fenofibrate HDL3.5–6 and HDL size3.5–6. Other haplotype not specified here showed no significantly association with any lipid phenotypes.

Table 4
Association tests of SCARB1 haplotypes with lipid phenotypes

DISCUSSION

In this study we systemically investigated the relationship between the SCARB1 gene and lipid profiles at the baseline and postprandial states, as well as lipid lowering treatment. Dietary fat challenges and pharmacologic intervention are two potent stimuli that produce large but highly variable changes in lipid profile (Suter et al. 2001; Durstine et al. 2001; Kuller et al. 2001). We undertook two interventions (i.e., dietary fat challenge and pharmacologic intervention), because by studying contexts that affect both axes (i.e., increasing and decreasing TGs) we may gain novel insight into underlying mechanisms that the SCARB1 gene may regulate the lipids. The most interesting finding was with the SNP SCARB1_G2S. The allele A of this SNP, compared to the alternative G, was associated with a higher response to fenofibrate by lowering TG levels and increasing plasma HDL-C levels (but the significance with HDL-C disappeared after adjusting for multiple testing).

Several previous studies examined association between the SCARB1 gene polymorphisms and lipid measures in different human populations. In a white population, male carriers of the 2 allele (1/2) of SCARB1_G2S had increased HDL-C and reduced LDL-C and of TGs concentrations at baseline (Acton et al. 1999). In a dietary intervention study, carriers of this allele appeared to be more responsive to changes in dietary saturated fat intake, as they exhibited a greater increase in LDL-C compared with 1/1 individuals (Perez-Martinez et al. 2003). In the Framingham Study, diabetic subjects carrying the SCARB1_G2S A allele had significantly lower LDL-C and HDL-C concentrations (Osgood et al. 2003). Our results reported herein are in agreement with these earlier findings. An important extension of the present study is that we for the first time found the SCARB1 gene is also related to lipid response to pharmacological (fenofibrate) treatment.

Mice studies showed that the genetic expression of SCARB1 modifies the metabolism of HDL-C. Overexpression of SCARB1 in the liver lowers plasma values for HDL-C and increases the concentration and biliary secretion of cholesterol (Kozarsky et al. 1997; Wang et al. 1998). In contrast, the total suppression of its expression increases plasma HDL-C and lowers the cholesterol content in bile and the suprarenal gland. After feeding a meal containing [14C] cholesterol and [3H] triolein, mice of overexpression of SCARB1 primarily in the intestine presented a rise in intestinal absorption of both lipids that was not due to a defect in chylomicron clearance nor to a change in the bile flow or the bile acid content (Bietrix et al. 2006), suggesting that SCARB1 acts as a multi-ligand transporter in the small intestine and is responsible for the accelerated uptake of cholesterol and TG hydrolysis products. The mechanism by which SCARB1 delivers cholesterol esters to cells is not well understood, but it appears to be different from the well characterized LDL receptor endocytic pathway (Brown and Goldstein 1986). It is likely that SCARB1 mediates uptake of lipids from both LDL and HDL, and this uptake is associated with an increase in cholesterol esterification (Stangl et al. 1998).

The physiological mechanism of SCARB1 in the postprandial lipemic response and pharmacologic intervention awaits further molecular/physiological studies to elucidate. Nonetheless, our findings from this study, along with those of others, suggest a potential mechanism that the SCARB1 gene polymorphisms may be related to a modified hepatic and/or intestinal expression of the SCARB1 gene, through which SCARB1 influences lipid absorption and clearance. In particular, the exon 1 polymorphism of SCARB1_G2S results in an amino acid change in the protein, which may modify receptor activity. Based on the current understanding of this exon 1variant, it is unlikely that it directly impairs selective cholesterol uptake. This is because selective cholesterol uptake resides primarily in the extracellular domain of the receptor, but the exon 1 amino acid change occurs in its intracellular N-terminus. Further in vitro and in vivo studies are needed to elucidate the exact functional effects of this SCARB1 exon 1 variant.

A major strength of this study lies in that the participants underwent rigorous interventions of both fat loading dietary challenge and fenofibrate trial, which provided a valuable resource to investigate the SCARB1 gene regarding its relationship with baseline lipid levels, postprandial lipid response and response to pharmacological treatment. Despite this asset, our study may have some limitations. First, although multiple SNPs were investigated across the gene, the marker density is not sufficient to cover the whole gene. To examine the gene more comprehensively, further studies by testing more SNPs, especially tagSNPs that cover all LDs in SCARB1, should be pursued. Second, a number of tests were performed due to the multiple lipid measures and SNPs involved. After correction for multiple testing, some identified associations only reached nominal significance or even disappeared. For example, the association between SCARB1_G2S and HDL-C in response to fenofibrate treatment turned out to be non-significant after adjustment for multiple testing. However, since the tested lipid measures are highly correlated and the tested SNPs are in LD, Bonferroni correction may be too conservative. Third, since our analyses were exploratory, the results await replication studies and eventually functional studies to validate. Due to the associated high costs and difficulties in compliance with the strict dietary and pharmacological interventions, we currently lack powerful independent datasets (including those from other groups) to replicate our study findings. We will pursue replication/confirmation in our future endeavors.

In summary, the present study suggests that the SCARB1 gene polymorphisms may serve as a useful marker to predict baseline lipid measures, postprandial lipid response as well as fenofibrate response. Our results may have significant clinical implications if confirmed by future studies.

Supplementary Material

Acknowledgments

We thank the families for their participation in this research. This work was supported by NIH Heart, Lung and Blood Institute grant U 01 HL72524, Genetic and Environmental Determinants of Triglycerides. We wish to acknowledge Abbott Laboratories (Abbott Park, IL) for their supply of study medication for this project. Dr. David B. Allison is supported by NIH grant 3P30DK056336. Dr. Kui Zhang is supported by NIH grant R01GM74913.

Footnotes

DUALITY OF INTEREST

By the present, we declare that we have no duality or conflict of interest.

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