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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Lancet. Author manuscript; available in PMC 2014 April 1.
Published in final edited form as:
PMCID: PMC3816716
NIHMSID: NIHMS517584

Acute lymphoblastic leukaemia

Summary

Acute lymphoblastic leukaemia (ALL) is seen in both children and adults, but its incidence peaks between ages 2 and 5 years. The causation of ALL is considered to be multi-factorial, including exogenous or endogenous exposures, genetic susceptibility, and chance. The survival rate of paediatric ALL has improved to approximately 90% in recent trials with risk stratification by biologic features of leukaemic cells and response to therapy, therapy modification based on patient pharmacodynamics and pharmacogenomics, and improved supportive care. However, innovative approaches are needed to further improve survival while reducing adverse effects. While most children can be cured, the prognosis of infants and adults with ALL remains poor. Recent genome-wide profiling of germline and leukaemic cell DNA has identified novel submicroscopic structural genetic alterations and sequence mutations that contribute to leukaemogenesis, define new ALL subtypes, influence responsiveness to treatment, and may provide novel prognostic markers and therapeutic targets for personalized medicine.

Introduction

An estimated 6000 new cases (3400 male and 2600 female) of acute lymphoblastic leukaemia (ALL) are diagnosed annually in the US.1 Patients are predominantly children; approximately 60% of cases occur at age <20 years.25 The survival rate of childhood ALL is approaching 90% (appendix, figure 1),4,6 although the treatment of infants and adults needs improvement.5,7 Here we review recent advances in the epidemiology, pathobiology, and clinical management of ALL.

Epidemiology

ALL, like cancer in general, is likely to arise from interactions between exogenous or endogenous exposures, genetic (inherited) susceptibility, and chance (figure 1). These factors account for the approximately 1 in 2000 risk of childhood (0–15 years) ALL. The challenge is to identify the relevant exposures and inherited genetic variants and to decipher how and when they contribute to the multi-step natural history of ALL from its initiation (usually in utero) through its largely covert evolution to overt disease.8 The task is complicated by the relative rarity of ALL and the existence of biologically distinct subtypes that may not share common aetiological mechanisms.9 For example, ALL in infants (<12 months) is usually associated with MLL gene rearrangement, and the remarkably high concordance rate in monozygotic twins (approaching 100% in those with a single or monochorionic placenta) suggests that leukaemogenesis is largely complete at birth.10 In contrast, non-MLL-rearranged B-ALL has a peak incidence between 2 and 5 years and a concordance rate of 10–15%, suggesting that, although initiation in utero is common, other “promotional” exposures are probably required for the later emergence of disease.10

Figure 1
Composite causality of childhood ALL. 1) Exogenous (eg, infection) and endogenous (eg, inflammation, oxidative stress) exposures, 2) normal allelic variation in inherited genes, and 3) chance all play roles in 4) the covert natural history of childhood ...

Exposures contributing to ALL

The topic of exposures and their role remains contentious. Epidemiological and case-control studies have found more than twenty candidate exposures that contribute to childhood ALL,11 but very few are based on reproducibly significant data or are biologically plausible. Some are of public concern, especially ionising and non-ionising (eg, electromagnetic field [EMF]) radiation. Ionising radiation is an established causal exposure for childhood ALL, as evidenced by the impact of the 1945 atomic bombs in Japan12 and by the modestly but significantly elevated risk caused by X-ray pelvimetry during pregnancy.13 These exposures are not currently relevant, although some argue that background or natural radiation could be significant.14 EMF exposures (eg, power lines) have been particularly controversial, and concern or confusion may be exacerbated by uncritical news reporting. Meta-analysis suggests a modestly elevated risk at high levels (>0.2μT),15 but the reliability of this finding is uncertain. It is impossible to prove that EMF never causes ALL, but at most, it might be involved in only a small minority of cases. Further, its credibility as a causal (promotional, late stage?) exposure is weakened by the lack of any known biological mechanism or credible modelling in vitro or in vivo.

Infection was the first suggested causal exposure for childhood ALL16 and remains the strongest candidate. Two specific hypotheses have been proposed, often referred to by their eponymous titles, and both are supported by epidemiological data (table 1).11,17,18 Both postulate that ALL results from an abnormal response to a common infection. The hypotheses differ in detail but are not mutually exclusive as explanations of rare time/space clusters of leukaemia19,20 or of ALL in the general community.11 There is no evidence to date of a unique or single transforming virus in ALL,11 as is the case of leukaemia in some animal species.21 Instead, it is likely that ALL is promoted indirectly by an abnormal or dysregulated immune response to one or more common infections (viral or bacterial) in a susceptible individual. Influenza viruses are plausible candidates.22 Susceptible children would be defined as having minimal prior exposure to infection during infancy and have a persistent in utero-generated pre-leukaemic clone11 plus a variable degree of genetic susceptibility, as described below. It is important to continue exploring the possible biological mechanisms of infectious promotion of ALL,23 as they could eventually lead to prophylactic interventions.

Table 1
Infectious hypotheses for childhood ALL

Inherited susceptibility

There is very little evidence of inherited predisposition to ALL via highly penetrant mutations in children or adults.24 The high concordance in identical twin children has a non-genetic explanation (blood cell chimaerism).10 Infants born with constitutive trisomy 21 or Down’s syndrome are, however, at substantially elevated risk of ALL (~40-fold at age 0–4 years) and acute myeloid leukaemia (AML).25 The apparent absence of familial clustering of ALL or of greatly elevated sibling risk (maximum 2-fold) does not, however, argue against inherited susceptibility. Previous attempts to identify inherited genetic susceptibility to ALL have used a candidate gene approach. These studies found interesting potential candidates in, for example, folate metabolism and the immune response, but most were statistically underpowered or not consistently reproducible.26 More recent genome-wide association studies (GWAS) of childhood ALL compare the whole genome (usually remission blood-derived DNA) in a large series of patients to that in an ethnically matched control group, focusing on single-nucleotide polymorphisms in DNA sequences (with ~80% genome coverage).2729 These studies demand hundreds or thousands of patients and controls, and, given the thousands of pan-genome markers being compared, a robust result is generally regarded as requiring a P value <10−7. They also require validation in a second, independent series of patients and independent confirmation by another group of researchers. To date, common allelic variants in four genes (IKZF1, ARID5B, CEBPE, and CDKN2A) have been significantly and consistently associated with childhood ALL (appendix, table 1).2729 It is likely that variants in other genes have odds ratios (or impact) of <1.2, but these can be identified only in very large cooperative studies (3000–5000 cases). The gene variants have additive effects, so that an individual inheriting one copy of a variant will have risk elevated at ~50% above the norm while one who inherits all four variants in homozygous form (or double dose) would have an approximately10-fold increase in the risk of ALL. The overall conclusion from these studies is that the risk of childhood ALL is influenced by co-inheritance of multiple low-risk variants. The lower incidence of B-ALL with high hyperdiploidy in African-American children in the US may at least partially reflect lower prevalence of the ARID5B risk allele (appendix, table 1).30 Inherited allelic variation may also affect response to treatment.31

A striking finding in these GWAS is the nature of the four genes implicated (appendix, table 1). Their products do not impinge directly on potential exposure pathways, such as immune or liver detoxification pathways, but rather are key regulators of blood cell development, proliferation, and differentiation. Further, acquired or somatic mutants of each of these genes have been detected in cases of ALL. This fact suggests that the inherited gene variants contribute to the intrinsic vulnerability of stem or precursor blood cells to transforming events either in utero at initiation and/or with subsequent post-natal promotion and clonal evolution (figure 1). There is suggestive evidence that the risk-conferring gene variants have lowered expression of their product,27 but their functional aspects remain to be explored. These data provide valuable new insights into the aetiology of childhood ALL, but, at the moment, they do not carry strong enough predictive value to merit screening of the whole population of children.

Pathobiology

Genetic basis of ALL

High-resolution profiling of genetic alterations has transformed our understanding of the genetic basis of ALL. It has been known for several decades that the majority of childhood ALL cases harbour gross chromosomal alterations (figure 2).32 In B-ALL, these include high hyperdiploidy with non-random gain of at least five chromosomes (including X, 4, 6, 10, 14, 17, 18, and 21), hypodiploidy with fewer than 44 chromosomes, and recurring translocations, including t(12;21)(p13;q22), encoding ETV6-RUNX1 (TEL-AML1); t(1;19)(q23;p13), encoding TCF3-PBX1 (E2A-PBX1); t(9;22)(q34;q11), encoding BCR-ABL1; MLL rearrangement involving 11q23 with a wide range of partner genes; and rearrangement of MYC into antigen receptor gene loci. Dysregulation of TAL1, TLX1, TLX3, and LYL1, particularly by rearrangement into T cell antigen receptor loci, is common in T-ALL. These alterations are of key importance in both the pathogenesis and clinical management of ALL (figure 3). Many chromosomal rearrangements disrupt genes that regulate normal hematopoiesis and lymphoid development (eg, RUNX1 and ETV6), activate oncogenes (eg, MYC), or constitutively activate tyrosine kinases (eg, ABL1). Several of these alterations are significantly associated with outcome, particularly in B-ALL, and are used in risk stratification. Notably, high hyperdiploidy and ETV6-RUNX1 are associated with favorable outcome, whereas low hypodiploidy, and MLL rearrangement (especially in infants and adults) are associated with a dismal prognosis, in both children and adults.

Figure 2
Cytogenetic and molecular genetic abnormalities in childhood ALL. ALL with rearrangement of CRLF2 but without the BCR-ABL1-like transcriptional profile rarely presents with other classifying karyotypic alterations but may be seen with high hyperdiploidy. ...
Figure 3
Schema of genetic pathogenesis of B-ALL at diagnosis and relapse. HSC, hematopoietic stem cell; RAG, recombinase activating gene.

However, many of these alterations alone do not induce leukaemia in experimental models, and many cases of ALL lack a gross chromosomal alteration, indicating that additional submicroscopic genetic alterations contribute to leukaemogenesis. High-resolution microarray profiling of DNA copy number alterations (deletions and gains) and sequencing have identified several novel structural genetic alterations and sequence mutations that define new subtypes of ALL, contribute to leukaemogenesis, and influence treatment responsiveness; in several cases, these are being explored as novel prognostic markers and therapeutic targets.3338 Importantly, many ALL subtypes are characterized by distinct constellations of structural genetic alterations that together drive establishment of the leukaemic clone.

Submicroscopic genetic alterations in ALL

More than 50 regions of recurring DNA copy number alteration have been identified in ALL.33,34,3943 Deletions are more common than amplification and typically are focal, often involving only a single gene. The nature and frequency of these alterations are associated with cell lineage (B or T) and cytogenetic ALL subtype. Notably, MLL-rearranged ALL, which is typically aggressive and arises early in life, harbors <1 additional genetic alteration per case, whereas ETV6-RUNX1 and BCR-ABL1 leukaemias manifest later in childhood and typically have at least 6–8 additional genetic alterations. Many of the involved genes encode regulators of lymphoid development, cell cycle, tumor suppressors, and lymphoid signaling molecules (figure 3, table 2). Most commonly altered are transcriptional regulators of B lymphoid development (eg, PAX5, IZKF1, and EBF1) in more than two-thirds of B-ALL cases and the CDKN2A/CDKN2B loci encoding the INK4/ARF tumor suppressors in more than 80% of T-ALL cases. While our understanding of sequence mutations in ALL is incomplete, existing data show that several genes are altered by multiple mechanisms, including deletion or amplification, sequence mutation, and translocation. The nature of genetic alteration is highly gene-dependent; for example, structural alterations are more common than sequence mutations in PAX5 and IKZF1 in B-ALL, whereas several genes (eg, WT1, PHF6, and NOTCH1 in T-ALL) are more commonly targeted by sequence alteration.

Table 2
Key genetic alterations in B-ALL

Several genetic alterations have well-established roles in leukaemogenesis, such as activating mutations in NOTCH1; however, the roles of many other genes remain unknown, in part because of the paucity of mouse models that faithfully recapitulate human ALL. However, several alterations are known to disrupt the activity of the encoded proteins in vitro or to result in dominant negative activity, and recent studies have shown that loss-of-function mutations in Pax544 and Ikzf145 accelerate the onset of B-ALL in mouse models.

Prognostic genetic alterations in ALL

As cytogenetic alterations alone do not accurately predict the risk of relapse, there is great interest in the relation between novel genetic alterations and ALL outcome. The most consistent association is that of IKZF1 alterations and poor outcome (table 2). IKZF1 encodes IKAROS, the founding member of a family of pleiotropic zinc finger-containing transcription factors, and is required for lymphoid lineage development (figure 3). IKZF1 is altered in 15% of B-ALL cases and is a hallmark of two high-risk ALL subtypes: BCR-ABL1 lymphoid leukaemia (either de novo or chronic myeloid leukaemia in lymphoid blast crisis)34,46 and a new subtype termed “BCR-ABL1-like ALL”, described below.47,48

IKZF1 alterations include loss-of-function deletions of the entire IKZF1 locus, focal deletions resulting in expression of dominant negative IKZF1 alleles, and less commonly, sequence mutations. Alteration of IKZF1 is associated with an increased risk of treatment failure and relapse in both BCR-ABL1-positive and -negative ALL; hence, there is considerable interest in testing for IKZF1 alterations at diagnosis, although current treatment protocols do not consider IKZF1 status in risk stratification.

Novel subtypes of ALL

Rearrangement of CRLF2

As many as 8% of childhood ALL cases have CRLF2 rearrangement at the pseudoautosomal region 1 (PAR1) of Xp/Yp (figure 2 and table 2). CRLF2 encodes cytokine receptor-like factor 2, the receptor for thymic stromal lymphopoietin (TSLP).36,37 The arrangement occurs either as a rearrangement of CRLF2 into the immunoglobulin heavy chain locus at 14q32, or as a focal deletion immediately upstream of CRLF2 that results in expression of a novel fusion, P2RY8-CRLF2. Both events dysregulate CRLF2 expression, resulting in increased expression by lymphoblasts that may be detected by diagnostic immunophenotyping. Less common is a p.Phe232Cys mutation that results in receptor dimerization.49 CRLF2 rearrangement is particularly common (>50% of cases) in ALL associated with Down syndrome (DS-ALL). In both DS and non-DS ALL, approximately 50% of cases with CRLF2-rearrangement harbor concomitant activating mutations in the Janus kinase genes JAK1 or JAK2 (table 2).36,37,50 Particularly in non-DS-ALL, CRLF2 and JAK alterations are also associated with deleterious IKZF1 alterations and poor outcome.51 The JAK mutations are located either at or near the active site of the JAK kinase domain or in the pseudokinase domains of JAK1/2, most commonly at p.Arg683 of JAK2. Importantly, JAK2 mutations common in ALL are distinct from those observed in myeloproliferative neoplasms.52 Co-expression of CRLF2 and JAK mutant alleles transforms model cell lines and activates downstream Jak-Stat signaling, suggesting that these alterations are co-transforming in B-ALL.37 In DS-ALL, alterations of IKZF1, but not of CRLF2 or JAK2, were associated with a worse prognosis.53

BCR-ABL1-like ALL

Ten to 12 percent of B-ALL cases exhibit a gene expression profile similar to that of BCR-ABL1 ALL but are BCR-ABL1-negative, commonly show IKZF1 alteration, and have a poor outcome (figure 2).47,48 As many as 50% of BCR-ABL1-like cases have CRLF2 rearrangements and JAK mutations. Next-generation sequencing, including transcriptome and whole-genome sequencing, has shown that the remaining cases harbour a diverse range of rearrangements, deletions, and sequence mutations that activate cytokine receptor and kinase signaling (eg, those involving ABL1, EPOR, IL7R, JAK2, and PDGFRB). Several of these were shown to be transforming in vitro and to activate kinase signaling in primary leukaemic cells.54

Intrachromosomal amplification of chromosome 21 (iAMP21) occurs in approximately 2% of BALL cases (figure 2).55 This entity was originally defined by fluorescence in-situ hybridization (FISH) as gain of at least three copies of RUNX1.56 Subsequent FISH and microarray studies showed that the region of amplification is typically large and complex and is often accompanied by deletion of the subtelomeric regions of chromosome 21.57 The functional consequences of iAMP21 are largely unknown, but identification is important as it is associated with poor outcome on standard-risk regimens.55,58

Genetics of relapse and clonal heterogeneity in ALL

Cytogenetic59 and genomic profiling of samples collected at diagnosis and relapse has shown that the majority of ALL cases exhibit substantial changes in the nature of genetic alterations during the disease course and that relapse often arises from the emergence of a minor subclone with genetic alterations distinct from those of the predominant clone at diagnosis (figure 3).6062 In most cases, the relapse clone shares lesions with the predominant clone at diagnosis, indicating a common “ancestral” or pre-leukaemic origin, but other lesions are discordant. Sensitive assays specific for individual alterations demonstrate that the relapse clone is often present at a low level at diagnosis, suggesting that the alterations that emerge at relapse confer resistance to therapy. Moreover, several of the alterations that most commonly emerge at relapse are those also associated with poor treatment outcome when present at diagnosis, such as deletions of IKZF1 and CDKN2A/CDKN2B. Less commonly, the relapse clone appears identical to or completely dissimilar to that at diagnosis. Similar findings were observed in candidate gene sequencing studies of relapsed ALL samples, which identified enrichment of loss-of-function mutations of the transcriptional coactivator and acetyltransferase CREBBP (encoding CREB-binding protein) and of TP53 (table 2, figure 3).38,63,64

Genome sequencing of ALL

Recent studies have shown that next-generation sequencing approaches are required to comprehensively identify the genetic alterations in leukaemia. Simultaneous sequencing of hundreds of thousands of nucleic acids (“massively parallel” sequencing) may be used to identify sequence mutations and structural variants in the encoding portion of the genome (“exome” sequencing), the transcriptome (mRNA sequencing), or the entire genome. A recent study sequenced 120 candidate genes and pathways targeted by DNA copy number alterations in 187 high-risk B-ALL cases and identified a high frequency of alterations targeting B lymphoid development (68%), the TP53/RB1 tumour suppressor pathway (54%), Ras signalling (50%), and Janus kinases (11%), as well as recurring mutations in genes including ETV6, TBL1XR1, CREBBP, MUC4, ASMTL, and ADARB2.65

Early T-cell precursor ALL (ETP-ALL) is an aggressive leukaemia characterized by an immature immunophenotype reminiscent of the murine thymic early T cell precursor,66 aberrant expression of myeloid and stem cell markers, a distinct gene expression profile, and dismal outcome.67 Whole genome sequencing of 12 ETP-ALL cases and mutational recurrence testing in an additional 94 ETP and non-ETP T-ALL cases showed that three pathways commonly mutated in AML were mutated at high frequency in ETP-ALL.68 These were inactivating mutations targeting haematopoietic and lymphoid development (including GATA3, ETV6, RUNX1 and IKZF1), mutations driving aberrant cytokine receptor and Ras signaling (NRAS, KRAS, FLT3, JAK1, JAK3 and IL7R), and deleterious mutations in chromatin-modifying genes, most notably components of the polycomb repressor complex 2 (PRC2) (EZH2, EED, and SUZ12). PRC2 normally mediates trimethylation of lysine 27 of histone 3 (H3K27), resulting in transcriptional repression; thus, these mutations are predicted to de-repress transcription. These results extend recent findings of PHF6 mutations in T-ALL which were obtained by sequencing X chromosome genes and explained increased incidence of T-ALL in males.69

These findings provide compelling evidence that whole-genome sequencing of the entire spectrum of ALL subtypes is required to identify all genetic alterations contributing to leukaemogenesis. In addition, studies investigating the nature of non-coding genetic mutations and the interaction of genetic, epigenetic, and transcriptomic factors in ALL will be of great interest.

Diagnosis

Morphological identification of lymphoblasts by microscopy and immunophenotypic determination of lineage commitment and developmental stage by flow cytometry are essential for correct diagnosis of ALL.2 Chromosomal analysis still plays an important role in the initial cytogenetic work-up. RT-PCR, FISH/multiplex ligation-dependent probe amplification, and flow cytometry are used to identify leukaemia-specific translocations, submicroscopic chromosomal abnormalities, and cellular DNA content, respectively. After genome-wide analysis becomes time- and cost-effective, it may replace many current diagnostic techniques. Appendix table 2 lists the tests that have prognostic and therapeutic implications.

Risk assignment

Clinical and biological factors

Age (infant or ≥10 years old), presenting leukocyte count (≥50×109/L), race (Hispanic or black), male sex, and T-cell immunophenotype have been considered adverse clinical prognostic factors in children, although their effect is diminished by contemporary risk-adapted therapy and improved supportive care.26 Infants with MLL rearrangement, especially those < 6 months old with a leukocyte count >300×109/L at diagnosis, still have a dismal prognosis.7 Cytogenetic and molecular risk factors have been discussed above.

Racial/ethnic differences in prognosis have been linked not only to socioeconomic factors but also to differences in genomic alterations.51,70,71 For example, germline single-nucleotide polymorphisms of PDE4B70 and ARID5B71 were shown to be associated with Native American genetic ancestry, and somatic CRLF2 rearrangements in ALL blasts51 were overrepresented in children from a Hispanic ethnic background; these alterations were found to contribute to inferior outcomes in Hispanics. Adverse prognosis conferred by genetic ancestry was mitigated by adding a course of delayed intensification therapy.70

Adolescents and adults have a greater prevalence of biologically high-risk leukaemia (eg, BCR-ABL1 and MLL rearrangement), a low incidence of favorable subtypes (eg, ETV6-RUNX1 and hyperdiploidy), and poorer adherence and tolerance to therapy.72 Older age (especially ≥60 years) and high presenting leukocyte count are also poor prognostic factors in this population. Recent studies showed that they had better outcomes when treated on paediatric rather than adult regimens.7276 Typically, paediatric regimens provide higher doses of non-myelosuppressive drugs, early and frequent intrathecal therapy, reinduction and long maintenance phases, and strict oversight of adherence. Appendix figure 2 shows ALL survival according to age group.

Response to therapy

Early treatment response is predictive of the risk of relapse and is used to assign patients to subsequent risk-adapted therapy.77 Methods that track residual leukaemic cells by flow cytometry (detecting aberrant immunophenotypes) and by PCR amplification (detecting leukaemia-specific immunoglobulin and T-cell receptor genes or fusion transcripts) allow the recognition of ALL cells present at levels well below those detectable by microscopic morphologic assessment, ie, minimal residual disease (MRD). MRD is currently the most powerful prognostic indicator in childhood and adult ALL, even in patients with low-risk features at presentation.6,7882 The kinetics of MRD clearance in response to identical remission-induction chemotherapy differed between B- and T-ALL; negative MRD on day 33 (after administration of 4 drugs) was the strongest prognostic factor in B-ALL,79 while negative MRD on day 78 (after 7 drugs) was also predictive in T-ALL, regardless of positive MRD on day 33.80

PCR is typically more sensitive than flow cytometry for measurement of MRD (~0.001% vs ~0.01%), and PCR-measurable low levels of MRD (0.001 to < 0.01%) after remission-induction therapy showed prognostic significance in childhood ALL.83 However, flow cytometry is faster, generally less expensive, and applicable to a larger proportion of patients,77 allowing early tailoring of therapy.81 The sensitivity of flow cytometry can be improved by using multi-color combinations of additional leukaemia-associated markers identified from differently expressed genes in ALL cells, yielding a detection threshold of ~0.001%.84

Treatment

Treatment of ALL typically spans 2–2.5 years, comprising 3 phases: remission-induction, intensification (or consolidation), and continuation (or maintenance).2 Most of the drugs used were developed before 1970. However, their dosage and schedule of administration in combination chemotherapy have been optimized on the basis of leukaemic-cell biological features, response to therapy (MRD), and patient pharmacodynamic and pharmacogenomic findings, resulting in the current high survival rate. Central nervous system (CNS)-directed therapy is administered to prevent relapse caused by leukaemia cells sequestered in this sanctuary site. Allogeneic haematopoietic stem-cell transplantation is considered for patients at very high risk. This section will focus on the most important advances in ALL treatment over the past 5 years.

Remission-induction therapy

Four to 6 weeks of remission-induction treatment eradicates the initial leukaemic cell burden and restores normal haematopoiesis in 96–99% of children and 78–92% of adults.25 The chemotherapy agents typically include a glucocorticoid (prednisone or dexamethasone), vincristine, and asparaginase, with or without anthracycline. This regimen appears to be sufficient for standard-risk ALL if intensified post-remission treatment is given. Patients at high or very high risk receive four or more drugs.

Prednisone (or prednisolone) has traditionally been used in ALL treatment, but dexamethasone is increasingly considered.85 However, the optimal doses and bioequivalence of these drugs are unclear. In prospective randomized trials, dexamethasone provided better control of CNS leukaemia and, at a prednisone-to-dexamethasone dose ratio <7, yielded better event-free survival, especially in children with T-ALL who responded well to prednisone pre-phase treatment and children age <10 years with B-ALL.8689 However, when a higher dose of prednisolone (dose ratio >7) was used, the two drugs showed no difference in efficacy.90,91 Glucocorticoid treatment is associated with adverse effects, including infection, osteonecrosis, fracture, psychosis, and myopathy, whose incidence is generally higher with dexamethasone than with prednisone. Thus, high-dose dexamethasone (eg, 10 mg/m2/day) is not recommended for adolescent B-ALL.92

Three preparations of asparaginase are currently available: Escherichia coli-derived, Erwinia caratovora-derived, and a monoethoxypolyethylene glycol succinimidyl conjugate of E. coli L-asparaginase (PEG-asparaginase).93 As these formulations have different half-lives (PEG-asparaginase > E. coli > Erwinia), it is crucial to maintain asparagine depletion by optimizing the dose intensity and schedule of the asparaginase used. PEG-asparaginase has largely replaced the native product, as it provides at least 2 weeks of therapeutic activity after a single dose and less frequently induces antibodies.94,95 Native E. coli and PEG-asparaginase activity were reported to be inversely related to anti-E. coli asparaginase antibody titers, although PEG-asparaginase was inhibited only at high antibody titers.96 Therefore, PEG-asparaginase may be considered when antibody titers are low to intermediate, and Erwinia asparaginase should be considered when titers are high. A significant pharmacokinetic interaction has been observed between glucocorticoid and asparaginase.97,98 Higher systemic exposure to asparaginase was found to be associated with higher exposure to dexamethasone, presumably because of impaired hepatic synthesis of proteins involved in dexamethasone clearance. Thus, anti-asparaginase antibodies can reduce exposure to both drugs and may increase the risk of relapse.98

Patients with BCR-ABL1-positive ALL have been considered to have a poor prognosis but benefit from early administration of a tyrosine kinase inhibitor (eg, imatinib, dasatinib). When this agent is added to multiagent chemotherapy, complete remission rates are >90% and event-free survival is superior to that of historical controls.99,100 Unlike imatinib, dasatinib targets both ABL1 and Src kinases; it also has more potent activity against BCR-ABL1, is active against imatinib-resistant BCR-ABL1 (except for T315I mutation), and has better CNS penetration.100102

Intensification (consolidation) therapy

Intensification (consolidation) therapy is administered after remission-induction to eradicate residual leukaemic cells.2,3 This phase commonly uses high-dose (ie, 1–8g/m2) methotrexate (MTX) with mercaptopurine, frequent pulses of vincristine and glucocorticoid, uninterrupted asparaginase for 20–30 weeks, and reinduction therapy with agents similar to those used during remission-induction.

The accumulation of the active MTX metabolites, MTX polyglutamates (MTXPG1–7), in leukaemic cells is associated with anti-leukaemic activity, the results of which can be affected by somatic and germline genetic factors, dose and duration of MTX administration, and leucovorin rescue. Functional enzyme and somatic genetic studies show that MTXPG1–7 accumulation varies widely among ALL subtypes, being low in TCF3-PBX1 ALL, T-ALL, and ETV6-RUNX1 ALL and high in hyperdiploid B-ALL, especially with gain of chromosome 18 or 10; therefore, the former group may benefit from higher MTX doses.103105 Germline single-nucleotide polymorphisms of the organic anion transporter polypeptide SLCO1B1 were found to be associated with high MTX clearance.106,107 In patients with high-risk ALL, high-dose MTX (5 g/m2, 4 doses, every 14 days) plus mercaptopurine was more effective than escalating-dose MTX (initial dose 100 mg/m2, increasing by 50 mg/m2, 5 doses, every 10 days) plus PEG-asparaginase, without increased acute toxicity.108 The duration of an effective serum MTX level is also important; accumulation of MTXPG1–7 was less with 4-hour infusions of high-dose MTX than with 24-hour infusions.109 Leucovorin rescue is required after high-dose MTX administration; however, its excessive use can counteract the anti-leukaemic effects of MTX and increase the risk of relapse.110

Reinduction therapy has proven to be a crucial element of ALL protocols. Intensified reinduction therapy with vincristine and asparaginase improved the outcome of patients with high-risk ALL.111 However, an identical second reinduction cycle did not improve the outcome of patients with high-risk ALL and a rapid marrow response to 7 days of induction therapy or of those with standard-risk ALL, suggesting that residual leukaemia clones after a course of reinduction therapy may represent intrinsic drug resistance.111,112 It is not clear whether this second reinduction cycle offers a benefit to patients with high-risk ALL and a slow early response, in the context of contemporary therapy. Osteonecrosis frequently occurs after reinduction therapy, especially in children 10 years and older. Alternative-week (10 mg/m2/day on days 0–6 and 14–20, 2 courses) rather than continuous (on days 0–20, 1 course) administration of dexamethasone significantly reduced osteonecrosis despite a higher cumulative dose.113

Hematopoietic stem cell transplantation and cellular therapy

Allogeneic haematopoietic stem cell transplantation (HSCT) is considered for children with very high-risk ALL and/or persistent disease.114 Contemporary HSCT protocols with high-resolution HLA typing, case-based conditioning, and improved supportive care have reduced relapse-related mortality, regimen-related toxicity, and infection.115,116 Further, survival is comparable regardless of stem cell source (matched related, matched unrelated, cord blood, or haploidentical donor).116118

In view of the ongoing development of disease detection and frontline therapies, the indications for allogeneic HSCT should be reassessed continuously. A level of MRD ≥10−4 before HSCT is strongly associated with relapse, and new strategies are needed to reduce the disease burden before and/or after HSCT.119,120 Patients with BCR-ABL1-positive ALL who obtain remission after multi-agent chemotherapy with ABL1 kinase inhibitors and young children (age <6 years) with B-ALL in delayed remission after induction failure can be treated without HSCT.99,100,121 The benefit of HSCT for infants with ALL is controversial; the role of HSCT, if any, is limited to a small high-risk group.122,123 Although many adult centers have considered HSCT during first complete remission a key element of therapy, treatment with paediatric-based regimens will decrease its use.5,72

Continuation therapy

Continuation therapy typically lasts 2 years or longer and comprises mainly daily mercaptopurine and weekly methotrexate with or without pulses of vincristine and dexamethasone.

Mercaptopurine and thioguanine are structural analogs of hypoxanthine and guanine, respectively, and inhibit de novo purine synthesis. Although thioguanine requires fewer steps to form the active metabolite thioguanine nucleotides and has greater in vitro cytotoxicity to lymphoblasts, randomized studies have not consistently shown a benefit of thioguanine in event-free survival124,125 or overall survival,126 and protracted doses •40 mg/m2/day were associated with death during remission, veno-occlusive disease, portal hypertension, and thrombocytopenia.124126 Thus, mercaptopurine is preferred for continuation therapy. Thiopurine methyltransferase (TPMT) catalyzes S-methylation of thiopurines to inactive methylated metabolites. Patients with homozygous or heterozygous TPMT deficiency experience moderate to profound myelosuppression when treated with thiopurines.127 They may also develop secondary malignancy, especially at higher doses (eg, mercaptopurine 75 mg/m2/day).128 Further, an adherence rate <95% to planned mercaptopurine doses is associated with relapse.129 Therefore, uninterrupted, pharmacogenetics-based mercaptopurine dosing is important.2 After thioguanine nucleotides are incorporated into DNA, DNA mismatch repair enzymes exert cytotoxicity. Deficiency of such enzymes (eg, MSH2) renders leukaemic cells thiopurine-resistant.130 MSH2 expression was low or undetectable in approximately 11% of children with newly diagnosed ALL due to partial or complete somatic deletion of genes that regulate MSH2 degradation (FRAP1, HERC1, PRKCZ, and PIK3C2B). These children experienced a high incidence of relapse.130

CNS-directed therapy

Control of CNS disease is a key component of ALL therapy. Prophylactic cranial irradiation (12–18 Gy) effectively controls disease, but its use has recently been reduced or eliminated to prevent acute neurotoxicity, neurocognitive deficits, endocrinopathies, secondary malignancies, and excess late mortality.131 The St. Jude Total XV6 and Dutch Childhood Oncology Group ALL-9132 protocols replaced cranial irradiation with triple intrathecal chemotherapy (methotrexate, hydrocortisone, and cytarabine) for all newly diagnosed patients; the 5-year cumulative risk of isolated CNS relapse was 2.7% and 2.6%, respectively, within the range achieved by prophylactic cranial irradiation (1.5–4.5%). Patients at high-risk of CNS relapse, comprising those with any CNS involvement (including leukemic-cell contamination by traumatic spinal tap) and/or T-ALL,6 should be treated with intensified intrathecal therapy during early remission-induction. Cranial irradiation can be reserved only for salvage treatment, as the retrieval rate is high for patients with an isolated CNS relapse who did not receive irradiation with initial treatment.6

In a randomized study in standard-risk ALL, triple intrathecal treatment reduced the frequency of CNS relapse compared with single-agent intrathecal methotrexate, but was associated with increased risk of bone marrow and testicular relapse, possibly due to less intensive systemic therapy.133 In St. Jude Total XV, not only excellent CNS outcomes but also excellent overall outcomes (5-year event-free survival, 85.6%; overall survival, 93.5%) were achieved.6 As CNS and hematologic relapses are competing events, systemic chemotherapy with high-dose methotrexate, intensive asparaginase, and dexamethasone, plus risk-based early intensive intrathecal chemotherapy, play a substantial role in preventing CNS relapse.131

Remaining questions and future directions

There remain subsets of ALL that carry an adverse prognosis. Further intensification of current regimens is unlikely to dramatically improve survival but is certain to increase short- and long-term adverse effects. Reduction of treatment intensity should be sought for patients at low risk. Studies of chronic health complications in the growing number of long-term adult survivors will help to refine therapy to reduce the toxicity of treatment, while functional genomics and proteomics will provide a deeper understanding of the epidemiology and pathogenesis of individual cases, allowing targeted “personalized medicine” (table 3). Although the majority of epidemiology and pathobiology studies have been developed for paediatric ALL, these should be expanded to adult patients.

Table 3
Targeted antileukaemic drugs in current clinical trials

Pharmacologic inhibitors of Janus kinases, such as ruxolitinib, are being explored in cases of childhood ALL harboring CRLF2 and JAK alterations.35 Detailed preclinical studies in BCR-ABL1-like ALL showed that the ABL1/PDGFRB inhibitors imatinib and dasatinib are effective against ALL cells with NUP214-ABL1 and that JAK inhibitors are effective against those with BCR-JAK2 or mutated IL7R; therefore, cases harboring these alterations may be candidates for targeted therapy.54 DNA and histone methyltransferase inhibitors and histone deacetylase inhibitors may reactivate silenced tumor-suppressor genes or augment sensitivity to concomitant chemotherapy. These epigenetic agents may be considered for infants with MLL-rearranged ALL, in whom aberrant DNA and histone methylation is frequently observed,134,135 and for patients with CREBBP mutations that encode histone acetyltransferase CREB-binding protein.38 Mutations in ETP-ALL are also observed in AML, suggesting that AML-directed therapies or agents that target JAK signaling may be beneficial.68 Monoclonal antibodies to surface antigens such as CD19, CD20, CD22, and CD52 have been used in unconjugated form (eg, rituximab and epratuzumab), conjugated to immunotoxins or chemotherapeutic agents (moxetumomab and inotuzumab ozogamicin), or in the form of a bispecific antibody (blinatumomab).136 The incorporation of rituximab into the hyper-CVAD regimen (fractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone) appears to improve outcome for younger adults (<60 years) with CD20-positive, BCR-ABL1-negative B-ALL.137 Blinatumomab, a bispecific, single-chain antibody to CD19 and CD3[sm epsilon], recruits and activates CD3 effector cytotoxic T cells and is cytotoxic to CD19-expressing target cells bound to the other arm of the antibody; it has shown activity against relapsed/refractory ALL in an adult phase II study.138

Emerging evidence shows that newly diagnosed ALL comprises multiple subclones and that chemoresistance is frequently driven by subpopulations harboring genetic alterations that confer resistance.6062 Thus, efforts should be made to identify patients at high risk of relapse by using highly sensitive methods to detect these subpopulations at diagnosis, and therapy should target these subpopulations to augment the efficacy of the current therapeutic regimen while reducing the intensity.

Search strategy and selection criteria

We searched Medline and PubMed for articles published between January 2007 and November 2012, using the keywords “acute lymphoblastic leukaemia”, “acute lymphocytic leukaemia”, and “acute lymphoid leukaemia”. Additional information was obtained from abstracts presented to the American Society of Hematology and the American Society of Clinical Oncology. We focused on publications from the past 5 years, but did not exclude commonly referenced and highly regarded older publications. We also searched the reference lists of articles identified by this search strategy and selected those we judged relevant. Review articles and book chapters are cited to provide readers with more details and more references than this Seminar can provide. Our reference list was modified on the basis of comments from peer reviewers.

Supplementary Material

01

Acknowledgments

Supported in part by grants from the National Institutes of Health (CA21765) (HI and CGM) and Leukaemia and Lymphoma Research UK (MG), and by the American Lebanese Syrian Associated Charities (ALSAC) (HI and CGM). CGM is a Pew Scholar in the Biomedical Sciences and a St Baldrick’s Scholar. These sponsors had no role in writing the Seminar. The authors thank Prof. Ching-Hon Pui, MD, for critical review and Sharon Naron, ELS, for editing the manuscript.

Footnotes

Contributors

All authors contributed to the writing of this paper and have seen and approved the final submitted version.

Conflicts of interest statement

HI receives unrelated research support from Bayer/Onyx.

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