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Appl Microbiol. May 1973; 25(5): 811–818.
PMCID: PMC380917
Metabolism of Nitrilotriacetate by Cells of Pseudomonas Species1
J. M. Tiedje, B. B. Mason, C. B. Warren, and E. J. Malec
Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48823
Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823
Monsanto Chemical Co., St. Louis, Missouri 63166
1 Published as Journal Article no. 6214 of the Michigan Agricultural Experiment Station.
A Pseudomonas species was isolated from soil which could degrade nitrilotriacetate (NTA) to CO2, H2O, NH3, and cellular constituents without the accumulation of significant quantities of intermediates either in the presence or absence of several inhibitors. After extensive gas chromatography analysis, small quantities of aspartate, glycine, and aconitate were the only detectable compounds to accumulate during NTA degradation, and these compounds were not excreted from the cells. Manometric studies indicated that iminodiacetate, glycine, and glyoxylate are possible intermediates, whereas N-methyliminodiacetate, sarcosine, and acetate are not. The data are consistent with an oxidative cleavage of the C—N bond of NTA as the initial degradation step.
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