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Logo of jbcThe Journal of Biological Chemistry
 
J Biol Chem. 2013 October 18; 288(42): 30607–30613.
Published online 2013 September 11. doi:  10.1074/jbc.C113.506097
PMCID: PMC3798531

Structural Insights on the Bacteriolytic and Self-protection Mechanism of Muramidase Effector Tse3 in Pseudomonas aeruginosa*

Abstract

The warfare among microbial species as well as between pathogens and hosts is fierce, complicated, and continuous. In Pseudomonas aeruginosa, the muramidase effector Tse3 (Type VI secretion exported 3) can be injected into the periplasm of neighboring bacterial competitors by a Type VI secretion apparatus, eventually leading to cell lysis and death. However, P. aeruginosa protects itself from lysis by expressing immune protein Tsi3 (Type six secretion immunity 3). Here, we report the crystal structure of the Tse3-Tsi3 complex at 1.8 Å resolution, revealing that Tse3 possesses one open accessible, goose-type lysozyme-like domain with peptidoglycan hydrolysis activity. Calcium ions bind specifically in the Tse3 active site and are identified to be crucial for its bacteriolytic activity. In combination with biochemical studies, the structural basis of self-protection mechanism of Tsi3 is also elucidated, thus providing an understanding and new insights into the effectors of Type VI secretion system.

Keywords: Crystal Structure, Peptidoglycan, Protein Complexes, Pseudomonas aeruginosa, Toxins, Antitoxins, Immune Protein Tsi3, Muramidase Effector Tse3, Type VI Secretion System

Introduction

Secretion systems are used by bacteria to deliver toxins/effectors evoking cell lysis, thus providing benefits for bacterial survival (1, 2). The recently reported Type VI secretion systems (T6SSs)4 in Pseudomonas aeruginosa, which facilitate the colonization of P. aeruginosa for its growth advantage, are also widely distributed in many bacterial species (38). P. aeruginosa kills neighboring bacterial cells by delivering toxic effectors through its tail-like T6SS apparatus directly into target cells, disrupting the processes of life cycles of target bacteria and expressing immune proteins for self-protection (815). For these T6SS effectors and their immune partners, several crystal structures were recently reported: Tse1 (13, 1619), Tae3 (20), and Tae4 (21, 22), possessing cell lysis activity by cleaving the γ-d-glutamyl-l-meso-diaminopimelic acid of peptidoglycan in the Gram-negative bacteria, were structurally characterized as endopeptidases with classical NlpC/P60 fold. Tse2 has been characterized as a toxin but with unknown mechanism, and its cognate partner Tsi2 is rationalized to be a cytoplasm neutralizer by physical interaction with Tse2 (2325). Tse3 is delivered to the periplasm of recipient bacteria and promotes cell lysis, and is characterized with muramidase activity that cleaves the β-1,4 bond between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan. However, P. aeruginosa itself could express periplasmic immune protein Tsi3 that neutralizes the lethal action of this toxin (11).

Here we report the first structural study of the Tse3-Tsi3 toxin/antitoxin complex at 1.8 Å resolution. Together with mutagenesis studies and a biochemical analysis, we provide structural insights into the bacteriolytic activity of Tse3 and further investigate how Tsi3 neutralizes Tse3 toxicity. Tse3 consists of two domains; the C-terminal catalytic domain adopts an extended goose-type lysozyme-like structure where Ca2+ ions play essential roles for its cell lysis activity. The immune protein Tsi3 interacts with Tse3 via a hydrogen-bond network and occupies the catalytic site of Tse3 to block the substrate binding. These studies are significant by not only contributing to a better understanding of T6SS secretion system in P. aeruginosa, but also by providing new insights into niche competition among pathogenic bacteria.

EXPERIMENTAL PROCEDURES

Cloning, Expression, and Purification of Proteins

Full-length Tse3 (residues 1–408) and the N-terminal signal peptide-deleted Tsi3 (residues 23–145, Tsi3-ΔN), respectively, were inserted into pET-15b between the restriction enzyme sites of NdeI/BamHI with a His6 tag and a thrombin cleavage site. Transformed BL21 (DE3) competent cells were cultured in LB medium at 37 °C and induced by 0.5 mm isopropyl-1-thio-β-d-galactopyranoside at 30 °C for 6 h. Bacterial cells were resuspended in buffer A (25 mm Tris-HCl (pH 8.2), 500 mm NaCl, 10 mm imidazole) and lysed by passing through a French press twice. Cell debris was removed by centrifugation at 16,000 × g for 60 min at 4 °C. The fusion protein was purified by nickel-nitrilotriacetic acid cartridge (Qiagen), and the His6 tag was removed by thrombin cleavage. Target proteins were further purified by size exclusion chromatography (Superdex-200, GE Healthcare) with buffer B (20 mm Tris-HCl (pH 8.2), 150 mm NaCl, 1 mm DTT). For Tse3-Tsi3-ΔN complex formation, Tse3 was mixed with excessive Tsi3-ΔN followed by gel filtration to remove the unbounded Tsi3-ΔN.

Crystallization, Data Collection, and Structure Determination

Hanging-drop vapor diffusion crystallization trials were set up at 16 °C. 2 μl of purified Tse3-Tsi3-ΔN (~10 mg/ml) was mixed with well solution (100 mm HEPES, 2 m NH4COOH, 50 μm CaCl2, pH 7.2) in a 1:1 ratio. The micro-seeding method was used to initiate crystal formation followed by macro-seeding two times to generate plate crystals with good diffraction. Crystals were flash-frozen with 25% glycerol in mother liquor for screening and data collection or derivatized in mother liquor with 1 m potassium iodide (KI) for 30 s before flash freezing. Back soaking was also applied to improve clarity.

Two crystal forms (C2 and P21) of the Tse3-Tsi3-ΔN complex were initially obtained, but only the P21 crystals diffracted very well and were used for final structure determination. Most crystals were anisotropic, so hundreds of crystals were screened on an in-house Rigaku x-ray source. High-resolution native datasets were collected at 100 K on the BL17U beamline of the Shanghai Synchrotron Radiation Facility (SSRF). The datasets were integrated and processed using crystalclear-1.44 (26) or HKL2000 (27) for Synchrotron. Phasing and auto model building were undertaken by SHELXC/D/E (28) and PHENIX suite (29). Model improvement and refinements were finalized in COOT (30) and REFMAC in CCP4 (31) (see Table 1).

TABLE 1
Data collection and refinement statistics of Tse3-Tsi3-ΔN complex

Isothermal Titration Calorimetry (ITC) Measurement

Experiments were performed using the iTC200 system (GE Healthcare) at 20 °C with titration buffer C (20 mm HEPES (pH 7.4), 100 mm NaCl). 80 μm His6 tag-removed Tse3 in 400 μl of titration buffer was placed in the sample cell, and 680 μm His6 tag-removed Tsi3-ΔN in 40 μl of titration buffer C was loaded into the injection syringe. A 120-s delay at the start of the experiment was followed by 20 injections containing 40 μl of the solution with 240-s intervals. All measured samples were stirred at 500 rpm. Blank injections of the Tsi3 protein into buffer C were subtracted from the experimental titration, and the data were analyzed in Origin7.0 (MicroCal®).

Whole-cell Lysis and Tse3 Activity Rescue Experiment

Overnight culture of Escherichia coli B strain was sub-inoculated into fresh LB medium and grown to the late logarithmic phase (A600 = 1.5). Harvested cells were washed and resuspended in permeabilization buffer E (0.5 m sucrose, 0.2% v/v Tween 20, and 40 mm HEPES (pH 6.8)). To prepare a Ca2+-free Tse3 sample, 0.8 mg/ml fresh purified Tse3 was incubated with 150 μm EDTA overnight in buffer F (10 mm HEPES (pH 7.2), 100 mm NaCl (99.999%)). Multiple steps of ultrafiltration using buffer F were applied to remove EDTA and metal ions. To prepare Ca2+-rescued Tse3, CaCl2 was added to the Ca2+-free Tse3 sample with a final concentration of 500 μm and incubated on ice for 3 h to let Ca2+ incorporate back into the protein.

For activity rescue experiments, the concentrations of wild type Tse3, Ca2+-free Tse3, and Ca2+-rescued Tse3 were adjusted to 1.5 mg/ml. 150 μl of each sample was added into 3 ml of E. coli B strain cell in permeabilization buffer E (A600 = 1.1), resulting in 1.7 μm Tse3, 7.5 μm EDTA, or 25 μm CaCl2 for rescue. The cell lysis rate was reflected by the decrease of A600 at 27 °C.

RESULTS

Crystal Structure of the Tse3-Tsi3 Complex

The crystal structure of Tse3-Tsi3-ΔN was determined by single isomorphous replacement with anomalous scattering (SIRAS) of the iodide derivative and refined to 1.8 Å without residues located in the outlier region by Ramachandran plot (Table 1). It contains full-length Tse3-(1–408) and Tsi3-ΔN-(23–145) with clear and continuous electron density map. The final model contains two Tse3-Tsi3-ΔN heterodimers per asymmetric unit. Therefore, only one of the Tse3-Tsi3-ΔN complexes is displayed and discussed in this study.

Tse3 is mainly composed of α-helices and short loops, which consist of a small N-terminal domain with a C-terminal catalytic domain (Fig. 1A). The N-terminal domain (residues 1–125) contains seven α-helices (α1-α7), which are packed in a rod-like helical repeat motif and tightly interacting with the C-terminal domain (Fig. 1B). The C-terminal catalytic domain (residues 147–408) contains two major lobes (Lobe1, Lobe2) forming an open, accessible catalytic groove with three Ca2+ binding cations (Fig. 1A).

FIGURE 1.
Overall structure of Tse3-Tsi3-ΔN complex. A, ribbon and surface representations of the Tse3-Tsi3-ΔN complex structure. Three loops of Tsi3 protruded to Tse3 and fully occupied the enzymatic groove (yellow, Tsi3 β-sheet; green ...

Tsi3 is mainly folded as a seven-stranded (β3–β9), highly curved antiparallel β-sheet, with a small β-sheet (residues 23–39, β1–β2) and α-helix (residues 133–142, α1-Tsi3) back-flanked on it (Fig. 1A). DALI search results show that Tsi3 shares structural similarities with Mog1p (Z score = 8.8, Protein Data Bank (PDB): 1EQ6) (32) and CyanoP (Z score = 7.4, PDB: 2LNJ) (33) but bears no functional relevance. In the Tse3-Tsi3-ΔN complex structure, three loops of Tsi3 protrude to Tse3 and fully occupy the enzymatic groove, and Arg-60 is located in the center of the loop and buried into the Tse3 enzymatic pocket (Fig. 1A).

The C-terminal domain of Tse3 interacts with N-terminal domain mainly via extensive hydrophobic interactions and hydrogen bonds. A long loop (residues 128–146), mainly composed of Ala and Gly (128AAAGATGVASQA139), links the N-terminal domain to the C-terminal domain. Phe-127 and Phe-144 anchor to the pocket of the core C-terminal domain and act as a hinge, providing the flexibilities to the N- and C-terminal domains (Fig. 1, A and B). The α7 helix (residues 108–125) is buried into the hydrophobic groove of the C-terminal domain and interacts with the N-terminal domain by forming hydrophobic interactions (Fig. 1, C and D). Two pairs of salt bonds (Asp-120 with Arg-80, Asp-113 with Arg-84) reinforce the interaction between α7 and the N-terminal domain (Fig. 1E). All of these interactions suggest that the α7 helix plays a central role for the structural integrity of the protein by linking the two domains in a position necessary for enzymatic activity.

DALI search results show that the overall fold of the N-terminal domain of Tse3 is similar to the eukaryotic ARM repeat and the HEAT repeat proteins (34). Sequence alignments of the N-domain with HEAT repeat proteins show that the N-terminal domain contains a set of conserved hydrophobic residues for interhelix interactions. Specifically, α1–α6 constitute the conserved ARM and HEAT-like repeat domain, but α7 in Tse3 is less conserved.

Tse3 Features a Goose-type Lysozyme-like Catalytic C Domain

The C-terminal domain of Tse3 is mainly composed of two lobes in a V-shape (Lobe1, residues 225–253 and 315–408; Lobe2, residues 147–221 and 255–314) (Fig. 2A). The enzymatic groove is in the center part of C-terminal domain, which is mainly formed by hydrophobic residues and hydrophilic residues with Ca2+-I and -II binding, thus forming a hydrophobic environment with negatively charged catalytic key residue Glu-250 (11) (Fig. 2B).

FIGURE 2.
Tse3 features a two-lobe catalytic C domain and Tsi3 obstructs the catalytic pocket. A, surface representations of the enzymatic groove from top and side views (yellow, Lobe1; green, Lobe2; red, conserved residues Glu-250 and Gln-280). B, top view of ...

In Tse3, only Lobe1 of the C-terminal domain shares high similarities to homologues, whereas Lobe2 is less conserved and specific. DALI results show that lytic transglycosylase MltE (Z score = 10.7, PDB: 2Y8P) (35), goose-type lysozyme (Z score = 10.0, PDB: 3GXK) (36) and lytic transglycosylase Slt70 (Z score = 9.8, PDB: 1QTE) (37) share structural similarities with the C-terminal domain. Sequence alignments and structure superimpositions (data not shown) indicate that only Lobe1 of the C-terminal domain adopts a goose-type lysozyme-like structure, confirming the known bacteriolytic muramidase activity of Tse3 (11).

There are mainly three types of lysozymes: chicken-type, goose-type, and lysozyme from T4 bacteriophage (36, 38, 39). The goose-type lysozymes adopt a α+β fold consisting of seven α-helices and three β-strands (36). In Tse3, the central helix α14 contains the highly conserved glutamic acid Glu-250 (Glu-73 in goose-type lysozyme, Glu-478 in Slt70, and Glu-64 in MltE), which is proposed to act as a general-acid catalyst (36). Another β-hairpin also exhibits high conservation, the conserved glutamine Gln-280 of Tse3 (Gln-99 in goose-type lysozyme, Gln-496 in Slt70, and Gln-82 in MltE) is located on this β-hairpin and coordinates with Ca2+ cations (Fig. 2B), although no metal ion binding was reported in these homologues (3537).

Tsi3 Interacts with Tse3 and Obstructs the Catalytic Pocket

An inspection of the electrostatic potential mapped onto the molecular surfaces reveals that the contact surfaces of Tse3-Tsi3-ΔN are complementary not only in shape, but also in electrostatic surface charge (Fig. 2B). Three loops of Tsi3 (Glu-55 to Gln-64 between β4 and β5, Asp-95 to Ser-102 between β7 and β8, and Ser-125 to Glu-129 between α1-Tsi3 and β9) bind in the enzymatic groove and interact with Tse3 by an extensive network of hydrogen bonds, with additional intramolecular hydrogen bonds between Gln-124, Asn-61, and Gly-101, fully occupying the enzymatic groove of Tse3 (Figs. 1A and and22B). More interestingly, the side chain of Arg-60 in Tsi3 forms a strong hydrogen-bond network with negatively charged residues (Glu-250, Asp-253, and Thr-377 from Tse3 and Ser-99 from Tsi3) and water molecules (H2O-I and H2O-II) and thus blocks the catalytic site of Tse3 (Fig. 2C). Single site mutation (R60A or R60E) of Tsi3 can completely abolish the interaction of Tse3-Tsi3-ΔN, which is verified by gel filtration (data not shown), confirming that Arg-60 plays a dominant role for Tsi3 immune function. The ITC results also show that wild type Tsi3-ΔN binds to Tse3 moderately with an equilibrium dissociation constant of 33 μm, hinting that the interaction between Tse3 and Tsi3 is weak (Fig. 2D).

Calcium Is Essential for Enzymatic Activity of Tse3

The most striking discovery in the Tse3-Tsi3-ΔN complex structure is that three calcium ions bind with Tse3; two calcium ions (Ca2+-I and Ca2+-II) bind adjacent to Glu-250 in the middle part of catalytic groove, and the third calcium ion (Ca2+-III) binds in the loop between helixes α21 and α22 (Figs. 1A and and22B). Ca2+-I coordinates with two water molecules (H2O-I, H2O-II) and four negatively charged residues from Tse3 (side chain of Asn-181, Gln-254, and Glu-258; main chain of Asp-253) (Figs. 2B and and33A). Ca2+-II also coordinates with four residues from Tse3 (side chain of Glu-258, Asp-262, and Gln-280; side chain and main chain of Ser-275) and the side chain of Glu-126 from Tsi3, with a distance of 4.3 Å to Ca2+-I (Figs. 2B and and33A). The third calcium ion (Ca2+-III) is coordinated with one water molecule (H2O-III) and 5 residues located in the 12-residue loop between α21 and α22 (side chain of Glu-375, Ser-378, and Asp-382; main chain of Arg-379 and Asn-384) (Figs. 2B and and33A).

FIGURE 3.
Ca2+ ions bind with Tse3 and play crucial role for the cell lysis activity of Tse3. A, hydrogen-bond network of Ca2+ binding in Tse3. H2O is in red; Ca2+ is in purple; residues of Tse3 are shown in cyan; Arg-60 and Glu-126 from Tsi3 are shown in yellow ...

The presence of calcium ions in the catalytic site led us to question whether they are enzymatic factors. Gel filtration results show that the Tse3-Tsi3-ΔN heterodimer could be completely disrupted in the presence of 7.5 μm EDTA, but adding 25 μm CaCl2 could rescue the formation of complex (Fig. 3B). The presence of calcium ions in the Tse3-Tsi3-ΔN complex was also experimentally confirmed by ICP-OES; the results show that calcium ions bind to the Tse3-Tsi3-ΔN complex with a molar ratio of 2:1 with 10 μm CaCl2 in solvent, but only 1:1 without 10 μm CaCl2 present (data not shown). In vitro whole-cell lysis assays also indicate that calcium ions are necessary for Tse3 enzymatic activity; wild type Tse3 could easily hydrolyze the peptidoglycan of bacteria and cause cell lysis, which is indicated by the fast dropping of the A600 absorption. In contrast, EDTA-treated Tse3 (Ca2+ free) lost this ability, but the addition of excess CaCl2 could rescue its cell lysis activity (Fig. 3C).

DISCUSSION

The structures of two T6SS toxins (Tse1 and Tae4) were recently reported. Both belong to the cysteine peptidase NlpC/P60 superfamily (16, 18, 19, 21, 22), whereas Tse3 contains an ARM/HEAT-like N-terminal domain and a goose-type lysozyme-like C-terminal catalytic domain (Fig. 1A). In the Tse3-Tsi3-ΔN complex structure described here, the interactions between the N- and C-terminal domains of Tse3 are mainly mediated by the α7 helix and Lobe2 (Fig. 1, B–E). The significant differences in the N-terminal domain and Lobe2 may introduce unique features of Tse3 and contribute to the regulation of enzymatic activity of the C-terminal domain. Considering that ARM- and HEAT-like motifs are commonly found to mediate protein-protein interactions by their modular recognition of extended peptide regions (34), it is reasonable to speculate that the N-terminal domain of Tse3 is crucial for the proper folding and conformational stabilities of the Tse3.

Although some enzymes that catalyze a hydrolytic reaction, such as phospholipase A2 and staphylococcal nuclease, bind with Ca2+ in their active site as a catalytic factor (40, 41), Ca2+ binding and involvement of enzymatic activity are not observed in the reported T6SS toxins (15, 16, 1822) or the goose-type lysozyme homologues (3537). Based on the high-resolution structure and biochemical studies presented here, we identified three Ca2+ ions that bind with Tse3; two Ca2+ ions bind directly in the catalytic site and adjacent to the catalytic residue Glu-250 (Figs. 2C and and33A), and the third Ca2+ ion binds in the loop between helixes α21 and α22. Structural superimpositions with homologues indicate that the structural features of Lobe2 in Tse3 mainly contribute to this unique Ca2+ binding ability. Enzymatic studies also show that Tse3 requires Ca2+ for cell lysis activity (Fig. 3C), demonstrating that muramidase effector Tse3 is different from other T6SS toxins. Furthermore, the ICP-OES results show that the binding molar ratio of calcium to Tse3-Tsi3-ΔN could vary from 2:1 to 1:1 (data not shown), strongly suggesting that the binding of Ca2+ ions to Tse3 is weak and dynamic. Although the Ca2+ ions are found to be essential for the enzymatic activity of Tse3, the details of peptidoglycan substrate binding and hydrolysis reaction mechanism still remain unclear and await to be addressed by further investigations.

Acknowledgments

We thank the staff of beamline BL17U at the SSRF for the technical assistance during data collection; we thank Prof. Olaf G. Wiest for proofreading; and we thank Yankui Lin, Feng Xiao, and Zhi Yan in the Shenzhen Entry-Exit Inspection and Quarantine Bureau for the instrumental assistance of ICP-OES elemental analysis.

*This work was supported by Ministry of Science and Technology (MOST) of China 2013CB911501 and NSFC31300600, Shenzhen Science and Technology Innovation (SZSTI) Program ZDSY20120614144410389 and JCYJ20120614150904060, and a startup fund from PKUSZ (to T. W.); NSFC21102007, SZSTI Program SW201110060 and SW201110018, and Peacock Program KQTD201103 (to Z. L.); and MOST of China 2012CB722602, NSFC20872004, NSFC20972008, and NSFC21290180 and SZSTI “Shuang Bai Project” (to D. Z. W.).

The atomic coordinates and structure factors (code 4LUQ) have been deposited in the Protein Data Bank (http://wwpdb.org/).

4The abbreviations used are:

T6SS
Type VI secretion system
ARM
armadillo
ICP-OES
inductively coupled plasma-optical emission spectrometer
ITC
isothermal titration calorimetry.

REFERENCES

1. Holland I. B. (2010) The extraordinary diversity of bacterial protein secretion mechanisms. Methods Mol. Biol. 619, 1–20 [PubMed]
2. Bleves S., Viarre V., Salacha R., Michel G. P., Filloux A., Voulhoux R. (2010) Protein secretion systems in Pseudomonas aeruginosa: A wealth of pathogenic weapons. Int. J. Med. Microbiol. 300, 534–543 [PubMed]
3. Cascales E. (2008) The type VI secretion toolkit. Embo. Rep. 9, 735–741 [PubMed]
4. Boyer F., Fichant G., Berthod J., Vandenbrouck Y., Attree I. (2009) Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics 10, 104. [PMC free article] [PubMed]
5. Jani A. J., Cotter P. A. (2010) Type VI secretion: not just for pathogenesis anymore. Cell Host Microbe 8, 2–6 [PMC free article] [PubMed]
6. Schwarz S., Hood R. D., Mougous J. D. (2010) What is type VI secretion doing in all those bugs? Trends Microbiol. 18, 531–537 [PMC free article] [PubMed]
7. Schwarz S., West T. E., Boyer F., Chiang W. C., Carl M. A., Hood R. D., Rohmer L., Tolker-Nielsen T., Skerrett S. J., Mougous J. D. (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6, e1001068. [PMC free article] [PubMed]
8. Mougous J. D., Cuff M. E., Raunser S., Shen A., Zhou M., Gifford C. A., Goodman A. L., Joachimiak G., Ordoñez C. L., Lory S., Walz T., Joachimiak A., Mekalanos J. J. (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312, 1526–1530 [PMC free article] [PubMed]
9. Leiman P. G., Basler M., Ramagopal U. A., Bonanno J. B., Sauder J. M., Pukatzki S., Burley S. K., Almo S. C., Mekalanos J. J. (2009) Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc. Natl. Acad. Sci. U.S.A. 106, 4154–4159 [PubMed]
10. Pell L. G., Kanelis V., Donaldson L. W., Howell P. L., Davidson A. R. (2009) The phage λ major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system. Proc. Natl. Acad. Sci. U.S.A. 106, 4160–4165 [PubMed]
11. Russell A. B., Hood R. D., Bui N. K., LeRoux M., Vollmer W., Mougous J. D. (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475, 343–347 [PMC free article] [PubMed]
12. Basler M., Pilhofer M., Henderson G. P., Jensen G. J., Mekalanos J. J. (2012) Type VI secretion requires a dynamic contractile phage tail-like structure. Nature 483, 182–186 [PMC free article] [PubMed]
13. Benz J., Sendlmeier C., Barends T. R., Meinhart A. (2012) Structural insights into the effector-immunity system Tse1/Tsi1 from Pseudomonas aeruginosa. PLoS One 7, e40453. [PMC free article] [PubMed]
14. Russell A. B., Singh P., Brittnacher M., Bui N. K., Hood R. D., Carl M. A., Agnello D. M., Schwarz S., Goodlett D. R., Vollmer W., Mougous J. D. (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe 11, 538–549 [PMC free article] [PubMed]
15. Dong T. G., Ho B. T., Yoder-Himes D. R., Mekalanos J. J. (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc. Natl. Acad. Sci. U.S.A. 110, 2623–2628 [PubMed]
16. Chou S., Bui N. K., Russell A. B., Lexa K. W., Gardiner T. E., LeRoux M., Vollmer W., Mougous J. D. (2012) Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system. Cell Rep. 1, 656–664 [PMC free article] [PubMed]
17. Shang G., Liu X., Lu D., Zhang J., Li N., Zhu C., Liu S., Yu Q., Zhao Y., Zhang H., Hu J., Cang H., Xu S., Gu L. (2012) Structural insight into how Pseudomonas aeruginosa peptidoglycanhydrolase Tse1 and its immunity protein Tsi1 function. Biochem. J. 448, 201–211 [PubMed]
18. Ding J., Wang W., Feng H., Zhang Y., Wang D. C. (2012) Structural insights into the Pseudomonas aeruginosa type VI virulence effector Tse1 bacteriolysis and self-protection mechanisms. J. Biol. Chem. 287, 26911–26920 [PMC free article] [PubMed]
19. Zhang H., Gao Z. Q., Su X. D., Dong Y. H. (2012) Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa. FEBS Lett. 586, 3193–3199 [PubMed]
20. Dong C., Zhang H., Gao Z. Q., Wang W. J., She Z., Liu G. F., Shen Y. Q., Su X. D., Dong Y. H. (2013) Structural insights into the inhibition of type VI effector Tae3 by its immunity protein Tai3. Biochem. J. 454, 59–68 [PubMed]
21. Zhang H., Zhang H., Gao Z. Q., Wang W. J., Liu G. F., Xu J. H., Su X. D., Dong Y. H. (2013) Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J. Biol. Chem. 288, 5928–5939 [PMC free article] [PubMed]
22. Benz J., Reinstein J., Meinhart A. (2013) Structural insights into the effector-immunity system Tae4/Tai4 from Salmonella typhimurium. PLoS One 8, e67362. [PMC free article] [PubMed]
23. Hood R. D., Singh P., Hsu F., Güvener T., Carl M. A., Trinidad R. R., Silverman J. M., Ohlson B. B., Hicks K. G., Plemel R. L., Li M., Schwarz S., Wang W. Y., Merz A. J., Goodlett D. R., Mougous J. D. (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe 7, 25–37 [PMC free article] [PubMed]
24. Zou T., Yao X., Qin B., Zhang M., Cai L., Shang W., Svergun D. I., Wang M., Cui S., Jin Q. (2012) Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded superhelical antitoxin. J. Mol. Biol. 417, 351–361 [PubMed]
25. Li M., Le Trong I., Carl M. A., Larson E. T., Chou S., De Leon J. A., Dove S. L., Stenkamp R. E., Mougous J. D. (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathog. 8, e1002613. [PMC free article] [PubMed]
26. Pflugrath J. W. (1999) The finer things in X-ray diffraction data collection. Acta Crystallogr D Biol. Crystallogr. 55, 1718–1725 [PubMed]
27. Otwinowski Z., Minor W. (1997) Processing of x-ray diffraction data collected in oscillation mode. Method Enzymol. 276, 307–326
28. Sheldrick G. M. (2008) A short history of SHELX. Acta Crystallogr. A 64, 112–122 [PubMed]
29. Adams P. D., Afonine P. V., Bunkóczi G., Chen V. B., Davis I. W., Echols N., Headd J. J., Hung L. W., Kapral G. J., Grosse-Kunstleve R. W., McCoy A. J., Moriarty N. W., Oeffner R., Read R. J., Richardson D. C., Richardson J. S., Terwilliger T. C., Zwart P. H. (2010) PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213–221 [PMC free article] [PubMed]
30. Emsley P., Lohkamp B., Scott W. G., Cowtan K. (2010) Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 [PMC free article] [PubMed]
31. Potterton E., McNicholas S., Krissinel E., Cowtan K., Noble M. (2002) The CCP4 molecular-graphics project. Acta Crystallogr. D Biol. Crystallogr. 58, 1955–1957 [PubMed]
32. Stewart M., Baker R. P. (2000) 1.9 Ä resolution crystal structure of the Saccharomyces cerevisiae Ran-binding protein Mog1p. J. Mol. Biol. 299, 213–223 [PubMed]
33. Jackson S. A., Hinds M. G., Eaton-Rye J. J. (2012) Solution structure of CyanoP from Synechocystis sp. PCC 6803: new insights on the structural basis for functional specialization amongst PsbP family proteins. Biochim. Biophys. Acta 1817, 1331–1338 [PubMed]
34. Andrade M. A., Petosa C., O'Donoghue S. I., Müller C. W., Bork P. (2001) Comparison of ARM and HEAT protein repeats. J. Mol. Biol. 309, 1–18 [PubMed]
35. Artola-Recolons C., Carrasco-López C., Llarrull L. I., Kumarasiri M., Lastochkin E., Martínez de Ilarduya I., Meindl K., Usón I., Mobashery S., Hermoso J. A. (2011) High-resolution crystal structure of MltE, an outer membrane-anchored endolytic peptidoglycan lytic transglycosylase from Escherichia coli. Biochemistry 50, 2384–2386 [PMC free article] [PubMed]
36. Helland R., Larsen R. L., Finstad S., Kyomuhendo P., Larsen A. N. (2009) Crystal structures of g-type lysozyme from Atlantic cod shed new light on substrate binding and the catalytic mechanism. Cell. Mol. Life Sci. 66, 2585–2598 [PubMed]
37. van Asselt E. J., Thunnissen A. M., Dijkstra B. W. (1999) High resolution crystal structures of the Escherichia coli lytic transglycosylase Slt70 and its complex with a peptidoglycan fragment. J. Mol. Biol. 291, 877–898 [PubMed]
38. Weaver L. H., Grütter M. G., Remington S. J., Gray T. M., Isaacs N. W., Matthews B. W. (1984) Comparison of goose-type, chicken-type, and phage-type lysozymes illustrates the changes that occur in both amino acid sequence and three-dimensional structure during evolution. J. Mol. Evol. 21, 97–111 [PubMed]
39. Strynadka N. C., James M. N. (1996) Lysozyme: a model enzyme in protein crystallography. EXS 75, 185–222 [PubMed]
40. Loll P. J., Lattman E. E. (1989) The crystal structure of the ternary complex of staphylococcal nuclease, Ca2+, and the inhibitor pdTp, refined at 1.65 Ä. Proteins 5, 183–201 [PubMed]
41. Steiner R. A., Rozeboom H. J., de Vries A., Kalk K. H., Murshudov G. N., Wilson K. S., Dijkstra B. W. (2001) X-ray structure of bovine pancreatic phospholipase A2 at atomic resolution. Acta Crystallogr. D Biol. Crystallogr. 57, 516–526 [PubMed]

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