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ACS Chemical Biology
 
From:
Published online 2013 July 8. doi: 10.1021/cb400411x

Table 1

Surface Plasmon Resonance Parametersa
ligand(s)enzymeka (M–1 s–1)kd (s–1)KD (μM)Rmax (RU)
UTPTbUAPN/AN/A83.1 ± 0.515.20 ± 0.02
GlcNAc-1-PTbUAPNDNDNDND
1TbUAP4.13 ± 0.100.11 ± 0.102.58 ± 0.0730.67 ± 0.20
1 + 500 μM UTPTbUAP2.24 ± 0.040.21 ± 0.039.30 ± 0.0910.21 ± 0.05
1 + 100 μM GlcNAc-1-PTbUAP5.71 ± 0.020.13 ± 0.042.35 ± 0.0326.16 ± 0.02
UTPHsUAPNDNDNDND
GlcNAc-1-PHsUAPbbb~35
1HsUAPNDNDNDND
aN/A: kinetic parameters are not available due to fast on-rate and off-rate; affinity was determined using equilibrium fit. ND: binding not detected.
bNo fit was possible due to complex binding kinetics.