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ACS Chemical Biology
Published online 2013 July 8. doi: 10.1021/cb400411x

Figure 1

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Surface plasmon resonance sensorgrams of TbUAP and HsUAP binding to substrates. (A) TbUAP binds UTP with a KD of 83.1 ± 0.5 μM, UTP was injected in duplicates at concentrations from 3.9 to 500 μM. The inset shows an equilibrium affinity fit to a 1:1 binding model. (B) HsUAP does not bind UTP. (C) TbUAP does not bind GlcNAc-1-P. (D) HsUAP binds GlcNAc-1-P with a complex binding profile, GlcNAc-1-P was injected in duplicates at concentrations from 9 nM to 20 μM. (E) The ordered sequential binding of substrates to UAP is reversed between TbUAP (solid arrows) and HsUAP (dashed arrows).

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