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Appl Microbiol. 1969 August; 18(2): 240–244.
PMCID: PMC377950

Response of White Mice to Cells and Culture Constituents of Clostridium perfringens1


Broth cultures of Clostridium perfringens (ATCC 10543) were fractionated by ammonium sulfate precipitation and Sephadex G-150 chromatography. Components isolated, as well as some enzymes present in the culture, were assayed for toxicity by feeding to white mice. Early work indicated that when a meat-fat-starch slurry, infected with C. perfringens, was fed to mice, the intestinal passage time was reduced. By using large numbers of mice as test animals and analyzing the data statistically, we found that C. perfringens and several fractions from the culture supernatant significantly affected the mice. A toxic material present in the supernatant was not identifiable as phospholipase C. Phospholipase C and physphorylcholine affected the intestinal passage time of the mice only when large amounts were given. The enzyme, neuraminidase, and another unidentified compound present in the supernatant affected the passage time when very small amounts were fed to mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • DISCHE FE, ELEK SD. Experimental food-poisoning by Clostridium welchii. Lancet. 1957 Jul 13;273(6985):71–74. [PubMed]
  • Duncan CL, Sugiyama H, Strong DH. Rabbit ileal loop response to strains of Clostridium perfringens. J Bacteriol. 1968 May;95(5):1560–1566. [PMC free article] [PubMed]
  • Hauschild AH, Niilo L, Dorward WJ. Clostridium perfringens type A infection of ligated intestinal loops in lambs. Appl Microbiol. 1968 Aug;16(8):1235–1239. [PMC free article] [PubMed]
  • Macchia V, Pastan I. Action of phospholipase C on the thyroid. Abolition of the response to thyroid-stimulating hormone. J Biol Chem. 1967 Apr 25;242(8):1864–1869. [PubMed]
  • Macfarlane MG, Knight BC. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins. Biochem J. 1941 Sep;35(8-9):884–902. [PubMed]
  • SMITH LD, GARDNER MV. The anomalous heat inactivation of Clostridium perfringens lecithinase. Arch Biochem. 1950 Jan;25(1):54–60. [PubMed]
  • VYSHEPAN ED, KRASNOVA TV. O roli proteoliticheskikh fermentov Bac. perfringens (tip A) v inaktivatsii obrazuemogo etimi bakteriiami alpha-toksina (letsitinazy C). Biokhimiia. 1954 Mar-Apr;19(2):202–207. [PubMed]
  • Weiss KF, Strong DH, Groom RA. Mice and monkeys as assay animals for Clostridium perfringens food poisoning. Appl Microbiol. 1966 Jul;14(4):479–485. [PMC free article] [PubMed]

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