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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell. Author manuscript; available in PMC 2013 September 15.
Published in final edited form as:
PMCID: PMC3773330
NIHMSID: NIHMS509222

Control of Developmental Regulators by Polycomb in Human Embryonic Stem Cells

SUMMARY

Polycomb group proteins are essential for early development in metazoans, but their contributions to human development are not well understood. We have mapped the Polycomb Repressive Complex 2 (PRC2) subunit SUZ12 across the entire nonrepeat portion of the genome in human embryonic stem (ES) cells. We found that SUZ12 is distributed across large portions of over two hundred genes encoding key developmental regulators. These genes are occupied by nucleosomes trimethylated athistoneH3K27, are transcriptionally repressed, and contain some of the most highly conserved noncoding elements in the genome. We found that PRC2 target genes are preferentially activated during ES cell differentiation and that the ES cell regulators OCT4, SOX2, and NANOG cooccupy a significant subset of these genes. These results indicate that PRC2 occupies a special set of developmental genes in ES cells that must be repressed to maintain pluripotency and that are poised for activation during ES cell differentiation.

INTRODUCTION

Embryonic stem (ES) cells are a unique self-renewing cell type that can give rise to the ectodermal, endodermal, and mesodermal germ layers during embryogenesis. Human ES cells, which can be propagated in culture in an undifferentiated state but selectively induced to differentiate into many specialized cell types, are thought to hold great promise for regenerative medicine (Thomson et al., 1998; Reubinoff et al., 2000; Mayhall et al., 2004; Pera and Trounson, 2004). The gene expression program of ES cells must allow these cells to maintain a pluripotent state but also allow for differentiation into more specialized states when signaled to do so. Learning how this is accomplished may be key to realizing the therapeutic potential of ES cells and further understanding early development.

Among regulators of development, the Polycomb group proteins (PcG) are of special interest. These regulators were first described in Drosophila, where they repress the homeotic genes controlling segment identity in the developing embryo (Lewis, 1978; Denell and Frederick, 1983; Simon et al., 1992; Orlando and Paro, 1995; Pirrotta, 1998; Kennison, 2004). The initial repression of these genes is carried out by DNA binding transcriptional repressors, and PcG proteins modify chromatin to maintain these genes in a repressed state (Duncan, 1986; Bender et al., 1987; Strutt et al., 1997; Horard et al., 2000; Hodgson et al., 2001; Mulholland et al., 2003).

The PcG proteins form multiple Polycomb Repressive Complexes (PRCs), the components of which are conserved from Drosophila to humans (Franke et al., 1992; Shao et al., 1999; Birve et al., 2001; Tie et al., 2001; Cao et al., 2002; Czermin et al., 2002; Kuzmichev et al., 2002; Levine et al., 2002). The PRCs are brought to the site of initial repression and act through epigenetic modification of chromatin structure to promote gene silencing (Pirrotta, 1998; Levine et al., 2004; Lund and van Lohuizen, 2004; Ringrose and Paro, 2004). PRC2 catalyzes histone H3 lysine-27 (H3K27) methylation, and this enzymatic activity is required for PRC2-mediated gene silencing (Cao et al., 2002; Czermin et al., 2002; Kuzmichev et al., 2002; Muller et al., 2002; Kirmizis et al., 2004). H3K27 methylation is thought to provide a binding surface for PRC1, which facilitates oligomerization, condensation of chromatin structure, and inhibition of chromatin remodeling activity in order to maintain silencing (Shao et al., 1999; Francis et al., 2001; Cao et al., 2002; Czermin et al., 2002).

Components of PRC2 are essential for the earliest stages of vertebrate development (Faust et al., 1998; O’Carroll et al., 2001; Pasini et al., 2004). PRC2 and its related complexes, PRC3 and PRC4, contain the core components EZH2, SUZ12, and EED (Kuzmichev et al., 2004; Kuzmichev et al., 2005). EZH2 is a H3K27 methyltransferase, and SUZ12 (Suppressor of zeste 12) is required for this activity (Cao and Zhang, 2004; Pasini et al., 2004). ES cell lines cannot be established from Ezh2-deficient blastocysts (O’Carroll et al., 2001), suggesting that PRC2 is involved in regulating pluripotency and self-renewal. Although the PRCs are known to repress individual HOX genes (van der Lugt et al., 1996; Akasaka et al., 2001; Wang et al., 2002; Cao and Zhang, 2004), it is not clear how these important PcG regulators contribute to early development in vertebrates.

Because the nature of PRC2 target genes in ES cells might reveal why PRC2 is essential for early embryonic development, pluripotency, and self-renewal, we have mapped the sites occupied by the SUZ12 subunit throughout the genome in human ES cells. This genome-wide map reveals that PRC2 is associated with a remarkable cadre of genes encoding key regulators of developmental processes that are repressed in ES cells. The genes occupied by PRC2 contain nucleosomes that are trimethylated at histone H3 lysine-27 (H3K27me3), a modification catalyzed by PRC2 and associated with the repressed chromatin state. Both PRC2 and nucleosomes with histone H3K27me3 occupy surprisingly large genomic domains around these developmental regulators and are frequently associated with highly conserved non-coding sequence elements previously identified by comparative genomic methods. The transcription factors OCT4, SOX2, and NANOG, which are also key regulators of ES cell pluripotency and self-renewal, occupy a significant subset of these genes. Thus, the model of epigenetic regulation of homeotic genes extends to a large set of developmental regulators whose repression in ES cells appears to be key to pluripotency. We suggest that PRC2 functions in ES cells to repress developmental genes that are preferentially activated during differentiation.

RESULTS AND DISCUSSION

Mapping Genome Occupancy in ES Cells

We mapped the location of both RNA polymerase II and the SUZ12 subunit of PRC2 genome-wide in human ES cells (Figure 1). The initiating form of RNA polymerase II was mapped to test the accuracy of the method and provide a reference for comparison with sites occupied by PRC2. The SUZ12 subunit of PRC2 is critical for the function of the complex and was selected for these genome-wide experiments. Human ES cells (H9, NIH code WA09) were analyzed by immunohistochemistry for characteristic stem cell markers, tested for their ability to generate cell types from all three germ layers upon differentiation into embryoid bodies, and shown to form teratomas in immunocompromised mice (Supplemental Data; Figures S1–S3).

Figure 1
Genome-Wide ChIP-Chip in Human Embryonic Stem Cells

DNA sequences bound by RNA polymerase II were identified in replicate chromatin-immunoprecipitation (ChIP) experiments using DNA microarrays that contain over 4.6 million unique 60-mer oligonucleotide probes spanning the entire nonrepeat portion of the human genome (Figure 1 and Supplemental Data). To obtain a probabilistic assessment of binding events, an algorithm was implemented that incorporates information from multiple probes representing contiguous regions of the genome, and threshold criteria were established to identify a data-set with minimal false positives and false negatives. RNA polymerase II was associated with the promoters of 7,106 of the approximately 22,500 annotated human genes, indicating that one-third of protein-coding genes are prepared to be transcribed in ES cells. Three lines of evidence suggest this dataset is of high quality. Most of the RNA polymerase II sites (87%) occurred at promoters of known or predicted genes. Transcripts were detected for 88% of the genes bound by RNA polymerase II in previous expression experiments in ES cells. Finally, independent analysis using gene-specific PCR (Supplemental Data) indicated that the frequency of false positives was approximately 4% and the frequency of false negatives was approximately 30% in this dataset. A detailed analysis of the RNA polymerase II dataset, including binding to miRNA genes, can be found in Supplemental Data (Tables S1S6 and Figures S4 and S5).

The sites occupied by SUZ12 were then mapped throughout the entire nonrepeat genome in H9 ES cells using the same approach described for RNA polymerase II (Figure 1C). SUZ12 was associated with the promoters of 1,893 of the approximately 22,500 annotated human genes, indicating that −8% of protein-coding genes are occupied by SUZ12 in ES cells (Supplemental Data; Tables S7 and S8). Independent site-specific analysis indicated that the frequency of false positives was approximately 3% and the frequency of false negatives was approximately 27% in this dataset.

Comparison of the genes occupied by SUZ12 with those occupied by RNA polymerase II revealed that the two sets were largely exclusive (Figure 1D; Supplemental Data; Table S8). There were, however, genes where SUZ12 and RNA polymerase II cooccupied promoters. At these genes, PRC complexes may fail to block assembly of the preinitiation complex (Dellino et al., 2004), consistent with the observation that Polycomb group proteins can associate with components of the general transcription apparatus (Breiling et al., 2001; Saurin et al., 2001).

The vast majority of SUZ12 bound sites were found at gene promoters (Figure 1E). Ninety-five percent of the SUZ12 bound regions were found within 1 kb of known or predicted transcription start sites (Supplemental Data and Table S7). This suggests that SUZ12 functions in human ES cells primarily at promoters rather than at distal regulatory elements. It is interesting that 40% of all SUZ12 bound regions are within 1 Kb of CpG islands (Table S7), given the recent discovery of a mechanistic link between PcG proteins and DNA methyltransferoses (Vire et al., 2006).

Global Transcriptional Repression by PRC2

PRC2 is composed of three core subunits, SUZ12, EED, and EZH2, and has been shown to mediate histone H3K27 methylation at specific genes in vivo. To confirm that SUZ12 is associated with active PRC2 at target genes, we used chromatin immunoprecipitation with antibodies against EED and the histone H3K27me3 mark and analyzed the results with promoter microarrays. We found that EED and the histone H3K27me3 mark cooccurred with SUZ12 at most genes using a high-confidence binding threshold (Figure 2). The false negative rates of thresholded data can lead to an underestimate of the similarity between different datasets. Plotting raw enrichment ratios for genes associated with SUZ12, EED, or H3K27me3 demonstrates that SUZ12 binding represents PRC2 binding at almost all target genes (Figure S6).

Figure 2
SUZ12 Is Associated with EED, histone H3K27me3 Modification, and Transcriptional Repression in ES Cells

Genetic and biochemical studies at selected genes indicate that PRC2-mediated H3K27 methylation represses gene expression, but it has not been established if it acts as a repressor genome-wide. If genes occupied by SUZ12 are repressed by PRC2, then transcripts from these genes should generally be present at lower levels in ES cells than in differentiated cell types. To test this prediction, we compared the expression levels of PRC2-occupied genes in four different ES cell lines with the expression level of these genes in 79 differentiated human cell and tissue types (Sato et al., 2003; Abeyta et al., 2004; Su et al., 2004). We found that PRC2 occupied genes were generally underexpressed in ES cells relative to other cell types (Figure 2C). A small fraction of the genes occupied by PRC2 were relatively overexpressed in ES cells (Figure 2C); these tended to show less extensive SUZ12 occupancy and were more likely to be cooccupied by RNA polymerase II (Supplemental Data). These results are consistent with the model that PRC2-mediated histone H3K27 methylation promotes gene silencing at the majority of its target genes throughout the genome in ES cells.

Key Developmental Regulators Are Targets of PRC2

Examination of the targets of SUZ12 revealed that they were remarkably enriched for genes that control development and transcription (Figure 3) and that SUZ12 tended to occupy large domains at these genes (Figure 4). Although only 8% of all annotated genes were occupied by SUZ12, ~50% of those encoding transcription factors associated with developmental processes were occupied by SUZ12. By comparison, RNA polymerase II preferentially occupied genes involved in a broad spectrum of cell proliferation functions such as nucleic acid metabolism, protein synthesis, and cell cycle (Figure 3A and examples in Figure 1B; Supplemental Data; Table S10).

Figure 3
Cellular Functions of Genes Occupied by SUZ12
Figure 4
SUZ12 Occupies Large Portions of Genes Encoding Transcription Factors with Roles in Development

It was striking that SUZ12 occupied many families of genes that control development and transcription (Figures 3B and S7 and Table S11). These included 39 of 40 of the homeotic genes found in the HOX clusters and the majority of homeodomain genes. SUZ12 bound homeodomain genes included almost all members of the DLX, IRX, LHX, and PAX gene families, which regulate early developmental steps in neurogenesis, hematopoiesis, axial patterning, tissue patterning, organogenesis, and cell-fate specification. SUZ12 also occupied promoters for large subsets of the FOX, SOX, and TBX gene families. The forkhead family of FOX genes is involved in axial patterning and tissue development from all three germ layers (Lehmann et al., 2003). Mutations in members of the SOX gene family alter cell-fate specification and differentiation and are linked to several developmental diseases (Schepers et al., 2002). The TBX family of genes regulates a wide variety of developmental processes such as gastrulation, early pattern formation, organogenesis, and limb formation (Showell et al., 2004). Thus, the genes preferentially bound by SUZ12 have functions that, when expressed, promote differentiation. This is likely to explain, at least in part, why PRC2 is essential for early development and ES cell pluripotency.

A remarkable feature of PRC2 binding at most genes encoding developmental regulators was the extensive span over which the regulator occupied the locus (Figures 4, S8, and S9). For the majority (72%) of bound sites across the genome, SUZ12 occupied a small region of the promoter similar in size to regions bound by RNA polymerase II (Figure 1). For the remaining bound regions, SUZ12 occupancy encompassed large domains spanning 2–35 kb and extending from the promoter into the gene. A large portion of genes encoding developmental regulators (72%) exhibited these extended regions of SUZ12 binding. In some cases, binding encompassed multiple contiguous genes. For instance, SUZ12 binding extended ~100 kb across the entire HOXA, HOXB, HOXC, and HOXD clusters but did not bind to adjacent genomic sequences, yielding a highly defined spatial pattern (Figure 4B). In contrast, clusters of unrelated genes, such as the interleukin 1-β cluster, were not similarly bound by SUZ12. Thus, genes encoding developmental regulators showed an unusual tendency to be occupied by PRC2 over much or all of their transcribed regions.

PRC2 and Highly Conserved Elements

Previous studies have noted that many highly conserved noncoding elements of vertebrate genomes are associated with genes encoding developmental regulators (Bejerano et al., 2004; Siepel et al., 2005; Woolfe et al., 2005). Given SUZ12’s strong association with this class of genes, we investigated the possibility that SUZ12 bound regions are associated with these highly conserved elements. Inspection of individual genes suggested that SUZ12 occupancy was associated with regions of sequence conservation (Figure 5A). Eight percent of the approximately 1,400 highly conserved noncoding DNA elements described by Woolfe and colleagues (Woolfe et al., 2005) were found to be associated with the SUZ12 bound developmental regulators (p-value 10−14). Using entries from the PhastCons database of conserved elements (Siepel et al., 2005), we found that SUZ12 occupancy of highly conserved elements was highly significant (using highly conserved elements with a LoD conservation score of 100 or better, the p-value for significances was less than 10−85). Since PRC2 has not been shown to directly bind DNA sequences, we expect that specific DNA binding proteins occupy the highly conserved DNA sequences and may associate with PRC2, which spreads and occupies adjacent chromatin. Thus, the peaks of SUZ12 occupancy might not be expected to precisely colocate with the highly conserved elements, even if these elements are associated with PRC2 recruitment.

Figure 5
SUZ12 Binding Is Associated with Highly Conserved Regions

Remarkably, the degree of the association between SUZ12 binding and conserved sequences increases when considering sequences with an increasing degree of conservation (Figure 5B). By comparison, RNA polymerase II showed no such enrichment. These results suggest that the subset of highly conserved noncoding elements at genes encoding developmental regulators may be associated with PcG-mediated silencing of these regulators.

Signaling Genes Are among PRC2 Targets

The targets of SUZ12 were also enriched for genes that encode components of signaling pathways (Figure 3A and Table S12). There is evidence that transforming growth factor-β (TGFβ), bone morphogenic protein (BMP), wingless-type MMTV integration site (Wnt), and fibro-blast growth factor (FGF) signaling pathways, which are required for gastrulation and lineage differentiation in the embryo, are also essential for self-renewal and differentiation of ES cells in culture (Loebel et al., 2003; Molofsky et al., 2004). SUZ12 generally occupied the promoters of multiple components of these pathways, but it occupied larger domains within a group of signaling genes that contained highly conserved elements. This group contained members of the Wnt family (WNT1, WNT2, WNT6) as well as components of the TGFβ superfamily (BMP2, GDF6). Recent studies have shown that Wnt signaling plays a role in pluripotency and self-renewal in both mouse and human ES cells (Sato et al., 2004), and our results suggest that it is important to maintain specific family members in a repressed state in ES cells.

Activation of PRC2 Target Genes during Differentiation

PRC2 is associated with an important set of developmental regulators that must be silent in ES cells but activated during differentiation. This observation suggests that PRC2 ultimately functions to repress occupied genes in ES cells and that these genes may be especially poised for transcriptional activation during ES cell differentiation. We reasoned that if this model is correct, genes bound by SUZ12 should be preferentially activated upon ES cell differentiation or in cells that lack SUZ12. Furthermore, in differentiated cells, SUZ12 might continue to be observed at silent genes but must be removed from genes whose expression is essential for that cell type.

We first examined gene expression in ES cells stimulated to undergo differentiation (Sato et al., 2003). We found that genes occupied by SUZ12 were more likely to be activated during ES cell differentiation than genes that were not occupied by SUZ12 (Figure 6A; Supplemental Data; Table S13), indicating that SUZ12-occupied genes show preferential activation during differentiation under these conditions. Thirty-six percent of genes bound by SUZ12 showed greater than 2-fold increases in expression during ES cell differentiation, whereas only 16% of genes not bound by SUZ12 showed such an increase. This effect was particularly striking at the set of developmental regulators (Figure 6B). SUZ12 occupied most (83%) of the developmental regulators that were induced more than 10-fold during ES cell differentiation.

Figure 6
Preferential Activation of PRC2 Target Genes during ES Cell Differentiation

We next examined the expression of SUZ12 target genes in Suz12-deficient cell lines derived from homozygous mutant blastocysts (Supplemental Data). We reasoned that genes bound by SUZ12 in human ES cells have orthologs in mice that should be upregulated in Suz12-deficient mouse cells, although we expected the overlap in these sets of genes to be imperfect because of potential differences between human and mouse ES cells, the possible repression of PRC2 target genes by additional mechanisms, and pleiotropic effects of the Suz12 knockout on genes downstream of Suz12-target genes. Differences in gene expression between Suz12 homozygous mutant cells and wild-type ES cells were measured using gene expression microarrays and the human SUZ12 binding data mapped to orthologous mouse genes using HomoloGene (www.ncbi.nlm.nih.gov/HomoloGene). We found that a significant portion of mouse genes whose counterparts were bound by SUZ12 in human ES cells were upregulated in Suz12-deficient mouse cells (70 of 346 genes, p = 6 × 10−4); these genes are listed in Table S14. Orthologs of genes occupied by SUZ12 in human ES cells were more likely to be activated and less likely to be repressed in Suz12-deficient mouse cells than orthologs of genes not occupied by SUZ12 (Figure 6C). Furthermore, we found that orthologs of Suz12 target genes that were induced upon human ES cell differentiation were generally also induced upon loss of Suz12 in mouse cells (Figure 6D). Genes that were activated during ES cell differentiation and in Suz12-deficient cells included those encoding transcriptional regulators (GATA2, GATA3, GATA6, HAND1, MEIS2, and SOX17) signaling proteins (WNT5A, DKK1, DKK2, EFNA1, EFNB1, EPHA4, and EPHB3) and the cell-cycle inhibitor CDKN1A. These data indicate that Suz12 is necessary to fully repress the genes that are occupied by PRC2 in wild-type ES cells and have since been confirmed with binding data and knockout studies of a second PRC subunit in mouse (Boyer et al., 2006).

If PRC2 functions to repress genes in ES cells that are activated during differentiation, then in differentiated tissues SUZ12 occupancy should be diminished at genes encoding developmental regulators that have a role in specifying the identity of that tissue, similar to results seen with Ezh2 at specific genes in mouse (Caretti et al., 2004). To test this, we designed an array focused on the promoters of developmental regulators and used ChIP-Chip to investigate SUZ12 occupancy at these promoters in primary differentiated muscle cells. The results demonstrated that genes encoding key regulators of muscle differentiation, including MYOD1, displayed greatly diminished SUZ12 occupancy when compared to ES cells (Figure 6E). MYOD1 is a master regulator for muscle differentiation (Tapscott, 2005), and the gene encoding this transcription factor displayed no significant SUZ12 occupancy when compared to the levels of SUZ12 occupancy observed in ES cells. Genes encoding other transcriptional regulators that play a central role in muscle development, such as PAX3 and PAX7 (Brand-Saberi, 2005), showed reduced levels of SUZ12 occupancy in muscle cells relative to ES cells (Supplemental Data and Figure S11). In contrast, other developmental regulators important for differentiation of nonmuscle tissues remained occupied by SUZ12 in differentiated muscle cells (Figure 6F and Table S15). These data support a model where PRC2 binding in ES cells represses key developmental regulators that are later expressed during differentiation.

Targets of PRC2 Are Shared with Key ES Cell Regulators

The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated ES cells in culture (Nichols et al., 1998; Avilion et al., 2003; Chambers et al., 2003; Mitsui et al., 2003). We recently reported that these transcription factors occupied promoters for many important developmental regulators in human ES cells (Boyer et al., 2005). This led us to compare the set of genes encoding developmental regulators and occupied by OCT4, SOX2, and NANOG with those occupied by PRC2 (Figure 7 and Supplemental Data). We found that each of the three DNA binding transcription factors occupied approximately one-third of the PRC2-occupied genes that encode developmental transcription factors (Figure 7A; Supplemental Data; Table S11). Remarkably, we found that the subset of genes encoding developmental regulators that were occupied by OCT4, SOX2, and NANOG and repressed in the regulatory circuitry highlighted in Boyer et al. were almost all occupied by PRC2 (Figure 7B). These included genes for transcription factors known to be important for differentiation into extraembryonic, endodermal, mesodermal, and ectodermal lineages (e.g., ESX1L, ONECUT1, HAND1, HOXB1). As expected, active genes encoding ES cell transcription factors (e.g., ZIC3, STAT3, OCT4, NANOG) were occupied by OCT4, SOX2, NANOG, and RNA polymerase II but not by PRC2 (Figure 7B).

Figure 7
SUZ12 Is Localized to Genes also Bound by ES Cell Transcriptional Regulators

The observation that OCT4, SOX2, and NANOG are bound to a significant subset of developmental genes occupied by PRC2 supports a link between repression of developmental regulators and stem cell pluripotency. Like PRC2, OCT4 and NANOG have been shown to be important for early development and ES cell identity. It is possible, therefore, that inappropriate regulation of developmental regulators that are common targets of OCT4, NANOG, and PRC2 contributes to the inability to establish ES cell lines in OCT4, NANOG, and EZH2 mutants (Nichols et al., 1998; O’Carroll et al., 2001; Chambers et al., 2003; Mitsui et al., 2003).

Concluding Remarks

We have mapped the sites occupied by SUZ12 throughout the genome to gain insights into how PRC2 contributes to pluripotency in human embryonic stem cells. ES cells proliferate in an undifferentiated state yet remain poised to respond to development cues. Genes encoding the transcriptional regulators that promote differentiation must therefore be repressed in ES cells but activated upon receiving signals to differentiate. We found that PRC2 occupies large domains at genes encoding a key set of repressed developmental regulators that are preferentially activated upon cellular differentiation, thus implicating this complex directly in the maintenance of the pluripotent state.

Transcription factors and chromatin regulators contribute to the transcriptional regulatory circuitry responsible for pluripotency and self-renewal in human ES cells. Understanding this circuitry is fundamental to understanding human development and realizing the therapeutic potential of these cells. In this context, we find it exciting that the outlines of the core transcriptional regulatory circuitry of human ES cells are emerging. The transcription factors OCT4, SOX2, and NANOG are associated with actively transcribed genes that contribute to growth and self-renewal (Boyer et al., 2005). These factors also occupy genes encoding key developmental regulators that are transcriptionally repressed, due at least in part to their association with PRC2 and nucleosomes modified at histone H3K27me3. Further study of transcription factors and chromatin regulators genome-wide will allow investigators to produce a more comprehensive map of transcriptional regulatory circuitry in ES cells and to test models that emerge from the circuitry. This information may provide insights into approaches by which pluripotent cells can be stimulated to differentiate into different cell types.

EXPERIMENTAL PROCEDURES

Cells and Cell Culture

Human H9 ES cells (WiCell, Madison, WI) were cultured as described (Boyer et al., 2005). Primary human skeletal muscle cells were obtained from Cell Applications (San Diego, CA) and expanded and differentiated into myotubes according to the supplier’s protocols. Suz12 −/− mouse cell lines were derived from blastocysts from crosses between heterozygous Suz12 mutant animals, as described in Supplemental Data.

Chromatin Immunoprecipitation and DNA Microarray Analysis

ChIP was combined with DNA microarray analysis as described (Boyer et al., 2005). The antibodies used here were specific for hypophosphorylated RNA polymerase II (8WG16) (Thompson et al., 1989), SUZ12 (Upstate, 07–379), EED (Hamer et al., 2002), H3K27me3 (Abcam, AB6002), and total histone H3 (Abcam, AB1791). The design of the oligobased arrays, which were manufactured by Agilent Technologies, is described in detail in Supplemental Data. A whole-chip error model was used to calculate confidence values from the enrichment ratio and the signal intensity of each probe (probe p-value) and of each set of three neighboring probes (probe-set p-value). Probe-sets with significant probe-set p-values (p < 0.001) and significant individual probe p-values were judged to be bound (see Supplemental Data for additional information). Bound regions were assigned to genes if they were within 1 kb of the transcription start site from one of five genomic databases; RefSeq, MGC, Ensembl, UCSC Known Gene, or H-Inv. All microarray data is available at ArrayExpress under the accession designation E-WMIT-7.

Gene Expression Analysis

Gene expression data were collated from H1 ES cells (Sato et al., 2003), H9, HSF1, and HSF6 ES cells (Abeyta et al., 2004), and 79 differentiated human cell and tissue types (Su et al., 2004) and analyzed as described in detail in Supplemental Data. Replicate gene expression data was obtained for wild-type mouse ES cells and Suz12-deficient cells using Agilent Mouse Development arrays and were analyzed as described in Supplemental Data.

Supplementary Material

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Acknowledgments

We thank Elizabeth Jacobsen for technical assistance and Robert Brady for help with array design. L.A.B. and H.L.M. were supported by NRSA postdoctoral fellowships. M.G.G. is an Amgen Fellow of LSRF. R.M.K. was supported by a fellowship from the ACS. D.T.O. was supported by NIH award DK070813. This work was supported by NIH grants HG002668 and GM069400. T.L., T.L.V., D.K.G., and R.A.Y. consult for Agilent Technologies.

Footnotes

Supplemental Data

Supplemental Data include fifteen figures, fifteen tables, Experimental Procedures, and References and can be found with this article online at http://www.cell.com/cgi/content/full/125/2/301/DC1/.

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