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Braz J Microbiol. 2011 Oct-Dec; 42(4): 1440–1444.
Published online 2011 December 1. doi:  10.1590/S1517-838220110004000029
PMCID: PMC3768747

Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Abstract

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.

Keywords: Bovine parainfluenza virus type 3, bPIV-3, phylogenetic analysis

Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier