PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of brazjmicroLink to Publisher's site
 
Braz J Microbiol. 2011 Oct-Dec; 42(4): 1485–1494.
Published online 2011 December 1. doi:  10.1590/S1517-838220110004000035
PMCID: PMC3768738

Application of response surface methodology to enhancement of biomass production by Lactobacillus rhamnosus E/N

Abstract

Response surface methodology (RSM) was employed to study the effects of various medium components on biomass production by Lactobacillus rhamnosus E/N. This strain is commonly used in the pharmaceutical and food industries due to its beneficial effect on the human gut and general health. The best medium composition derived from RSM regression was (in g/l) glucose 15.44, sodium pyruvate 3.92, meat extract 8.0, potassium phosphate 1.88, sodium acetate 4.7, and ammonium citrate 1.88. With this medium composition biomass production was 23 g/l of dry cell weight after 18 h of cultivation in bioreactor conditions, whereas on MRS the yield of biomass was 21 g/l of dry cell weight. The cost of 1 g of biomass obtained on MRS broth was calculated at the level of 0.44 € whereas on the new optimal medium it was 25% lower. It may be concluded then, that the new medium, being cheaper than the control MRS allows large scale commercial cultivation of the L. rhamnosus strain. This study is of relevance to food industry because the possibility to obtain high yield of bacterial biomass is necessary step in manufacturing of probiotic food.

Keywords: response surface methodology, Lactobacillus rhamnosus, medium selection, biomass production

Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier