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Braz J Microbiol. 2011 Oct-Dec; 42(4): 1334–1342.
Published online 2011 December 1. doi:  10.1590/S1517-838220110004000014
PMCID: PMC3768732

Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp.

Abstract

Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50°C and 6 respectively. The enzyme was found to be thermostable at 70°C for about 2 h without any salt. It showed stability at pH range 5–7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.

Keywords: Solid State Fermentation, Agro-industrial wastes, Amylase, Enzyme optimization, Stability, Bacillus sp.

Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier